Jiangsu Engineering Research Center for Biomedical Function Materials

Nanjing, China

Jiangsu Engineering Research Center for Biomedical Function Materials

Nanjing, China

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Zhang M.,Nanjing Normal University | Zhang M.,Jiangsu Engineering Research Center for Biomedical Function Materials | Yuan P.,Nanjing Normal University | Yuan P.,Jiangsu Engineering Research Center for Biomedical Function Materials | And 9 more authors.
RSC Advances | Year: 2017

In this study, doxorubicin (DOX) hydrochloride as a model drug, N-doped carbon dots as a drug carrier, and heparin as an auxiliary medicine were selected to design and prepare a multi-functional drug delivery system with pH-triggered drug release. The CDs were anchored onto heparin via chemical bonds and DOX was then loaded on CDs-Hep by taking advantage of the electrostatic interactions between DOX and CDs-Hep. The structures of all the intermediates and final products were characterized and confirmed by 1H NMR and FT-IR spectroscopies. The CDs-Hep/DOX drug delivery system exhibited good stability. However, in acidic buffer, Hep and DOX release rate was accelerated and it was pH-responsive. In vitro and in vivo studies confirmed the high biocompatibility and low-toxicity of the CDs. An MTT assay showed that inhibition rate of CDs-Hep/DOX for HeLa, MCF-7 and A549 cells was close to that of DOX, indicating that the prepared drug system has a higher toxicity for tumor cells and can achieve an effective therapeutic effect. This systemic evaluation suggests that the introduction of Hep improves blood compatibility. In addition, the internalization of CDs-Hep/DOX by A549 cells was further confirmed using laser scanning confocal microscopy. As a result, a therapy was achieved due to the incorporation of Hep and DOX. © The Royal Society of Chemistry.


Zhang M.,Nanjing Normal University | Zhang M.,Jiangsu Engineering Research Center for Biomedical Function Materials | Zhou N.,Nanjing Normal University | Zhou N.,Jiangsu Engineering Research Center for Biomedical Function Materials | And 9 more authors.
RSC Advances | Year: 2017

A folate-functionalized carbon dot-based nanocarrier system has been successfully synthesized for cancer cell targeted drug delivery. We hydrothermally synthesized blue photoluminescent N,P-CDs using adenosine triphosphate and graphene oxide as the starting materials. The particle size of the N,P-CDs was ca. 3.8 nm. An anticancer drug, doxorubicin (DOX), was grafted onto the carbon dots via electrostatic interactions, and a more specific anticancer agent (DOX/N,P-CDs) was obtained. The DOX/N,P-CDs were characterized by H-NMR, C-NMR and UV-vis analysis. In addition, the DOX/N,P-CDs showed a pH-dependent release and were easily absorbed by cells. When compared to DOX, DOX/N,P-CDs nanoparticles exhibited the same cytotoxicity towards human cervical cancer cells (HeLa cells) and human pulmonary adenocarcinoma cells (A549 cells). Hemolysis test results indicated that DOX/N,P-CDs were safe for blood-contact applications and were suitable for intravenous administration. Owing to their intrinsic biocompatibility, N,P-CDs can be used for cell imaging and drug delivery with excellent targeting property. © The Royal Society of Chemistry.


Zhang M.,Nanjing Normal University | Zhang M.,Jiangsu Engineering Research Center for Biomedical Function Materials | Chi C.,Nanjing Normal University | Chi C.,Jiangsu Engineering Research Center for Biomedical Function Materials | And 9 more authors.
Materials Science and Engineering C | Year: 2017

In this work, phosphor and nitrogen co-doped carbon dots (N-P-doped CDs) were developed for bioimaging. The as-synthesized N-P-doped CDs emit a bright blue coloured fluorescence after exposure to a 365 nm UV-lamp illumination. It is also demonstrated that the fluorescence of CDs is resistant to the interference of metal ions, saline solution, and high ionic strength environments. The bright fluorescence nature of the N-P-doped CDs has proven them to be excellent probes for cellular imaging. And this guess is further confirmed by using a laser scanning confocal microscope (LSCM). The viability study was carried out by MTT assay, suggesting the high biocompatibility of N-P-doped CDs. The results demonstrated that N-P-doped CDs may be considered as a safe material for bio-imaging and drug delivery in cancer cells. © 2017 Elsevier B.V.


Tan J.,Nanjing Normal University | Tan J.,Jiangsu Engineering Research Center for Biomedical Function Materials | Meng N.,Nanjing Normal University | Meng N.,Jiangsu Engineering Research Center for Biomedical Function Materials | And 11 more authors.
Materials Science and Engineering C | Year: 2016

A novel drug carrier based on hydroxypropyl-β-cyclodextrin (HP-β-CD) modified carboxylated graphene oxide (GO-COOH) was designed to incorporate anti-cancer drug paclitaxel (PTX). The formulated nanomedicines were characterized by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Results showed that PTX can be incorporated into GO-COO-HP-β-CD nanospheres successfully, with an average diameter of about 100 nm. The solubility and stability of PTX-loaded GO-COO-HP-β-CD nanospheres in aqueous media were greatly enhanced compared with the untreated PTX. The results of hemolysis test demonstrated that the drug-loaded nanospheres were qualified with good blood compatibility for intravenous use. In vitro anti-tumor activity was measured and results demonstrated that the incorporation of PTX into the newly developed GO-COO-HP-β-CD carrier could confer significantly improved cytotoxicity to the nanosystem against tumor cells than single application of PTX. GO-COO-HP-β-CD nanospheres may represent a promising formulation platform for a broad range of therapeutic agent, especially those with poor solubility. © 2016 Elsevier B.V. All rights reserved.


Tang Y.-D.,Jiangsu Engineering Research Center for Biomedical Function Materials | Tang Y.-D.,Nanjing Normal University | Zhou N.-L.,Jiangsu Engineering Research Center for Biomedical Function Materials | Zhou N.-L.,Nanjing Normal University | And 9 more authors.
Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities | Year: 2012

Carboxyl modifiedgraphene oxide is important in the preparation of functionalized graphene oxide(GeneO). In this work, the free radical initiator agent azobisisobutyronitrile(AIBN) was functioned as a modifier. AIBN could be separated into isobutyronitrile radicals to attack the five-membered ring and seven-membered ring defects in the graphene oxide. Then the cyanogroup-modified graphene oxide intermediates were formed, and the carboxyl graphene oxide[GeneO-C(CH3)2-COOH] could be obtained through the hydrolysis reaction. The structure and properties of GeneO-C(CH3)2-COOH were characterized by Fourier transform infrared spectroscope(FTIR), X-ray diffraction(XRD), thermo gravimetric analyzer(TGA) and atomic force microscope(AFM). And the blood compatibility of GeneO-C(CH3)2-COOH was evaluated by recalcification time test. It was showed that the recalcification time gradually decreased as the increasing concentration of GeneO-C(CH3)2-COOH, indicating that the material had good blood compatibility. Carboxyl group content could be controlled by the mass ratio of AIBN to GeneO. This method not only could increase the carboxyl content of oxidized graphene, but also made the material have a good blood compatibility.

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