Jiangsu Dafeng Milu National Nature Reserve

Dazhong, China

Jiangsu Dafeng Milu National Nature Reserve

Dazhong, China

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Zhai Y.,Nanjing University | Zhu Z.,Nanjing University | Zhu Y.,Nanjing University | Qian D.,Nanjing University | And 5 more authors.
Molecules | Year: 2017

First documented in Shennong Bencao Jing (about 200 B.C.-200 A.D.), Elaphuri Davidiani Cornu (EDC) has been recorded for its effects in strengthening bones and balancing other aspects of overall health for approximately 2000 years. In the present study, our aim was to investigate which are the components of the active EDC fraction by a peptidomic strategy. We explored the extent to which EDC increases the proliferation of osteoblasts by measuring the elevations in osteonectin and type I collagen mRNA levels and characterized it using nano-flow liquid chromatography in tandem with orbitrap mass spectrometry. In total, 272 peptide sequences from collagens were determined. "Hot regions" in parent proteins determined by peptide heat maps which indicated that amino acid sequences in the regions might undergo proteolysis easily and generate peptides. Among the identified peptides, 90.2% were hydrophilic, and the molecular weight of 97.1% of identified peptides was lower than 2000 Da. According to these results, EDC collagen-derived peptides were easily analyzed and identified. Moreover, this methodology is feasible to characterize the active peptides matrices originated from collagen hydrolysates or some other animal horn- derived TCMs. © 2017 by the authors; licensee MDPI, Basel, Switzerland.


Li F.-T.,Jiangsu Agri Animal Husbandry Vocational College | Li F.-T.,Nanjing University | Duan J.-A.,Nanjing University | Qian D.-W.,Nanjing University | And 5 more authors.
Chinese Traditional and Herbal Drugs | Year: 2015

As a precious Chinese medicinal resource, the antler, bone, flesh, and blood of Elaphurus davidianus (elk) have been used in Chinese medicine for over 2 000 years. However, they had been out of use for more than 100 years since the deer was extinct in the wild in China. Nowadays, as the reintroduction of elk population in China, they have increased rapidly and aroused a great interest in studies on the protection and utilization of biological resources. Based on analysis of the development and conservation of the elk resources, the strategies of resources industrialization of elk biological resources were put forward. Firstly, the combination by development model of wild domestication and artificial breeding should be established. Secondly, basic researches on the rational utilization of the elk resources need to be carried out. Thirdly, a series of products originated from Milu resources should be developed. All these strategies could promote the resources industrialization of elk in agriculture, industry, commerce, health care products, food, cosmetics, and industrial processing equipment and technology of Chinese medicine. They could also guide and promote the comprehensive utilization and protection of elk biological resources for sustainable development. ©, 2015, Editorial Office of Chinese Traditional and. All right reserved.


Li F.,Nanjing University | Li F.,Jiangsu Agri animal Husbandry Vocational College | Duan J.-A.,Nanjing University | Qian D.,Nanjing University | And 7 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2013

A rapid and sensitive hydrophilic interaction liquid chromatography method was developed for the identification and quantitative determination of 17 nucleosides and nucleobases by using ultra-high performance liquid chromatography separation system coupled to triple quadrupole mass spectrometer. Sufficient separation of the target compounds was achieved on an Acquity BEH Amide column (2.1mm×100mm, 1.7μm) in a single run of 9min. The LOD and LOQ were in the range of 0.017-50.00ngml-1 and 0.056-146.00ngml-1, respectively. Then, the method was applied for the analysis of nucleosides and nucleobases in Elaphuri Davidiani Cornu and Cervi Cornu. Multivariate statistical analysis was also used to investigate the differences in the nucleosides and nucleobases profiles among the samples. The present study also provided helpful information for quality control of Elaphuri Davidiani Cornu and Cervi Cornu. © 2013 Elsevier B.V.


PubMed | Jiangsu University, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu Dafeng Milu National Nature Reserve and Nanjing University
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2017

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