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Liu T.,Nanjing University of Technology | Jiang L.-L.,Nanjing University of Technology | He M.-F.,Nanjing University of Technology | Zhu Z.,Nanjing University of Technology | And 10 more authors.
RSC Advances | Year: 2015

Low toxic and highly biocompatible graphene-based nanomaterials are in high demand within the biomedical fields. In this study, a highly biocompatible bacterially reduced graphene oxide (BRGO) was prepared by a "Generally Recognized As Safe" (GRAS) strain Bacillus subtilis 168 mediated with Vitamin K3 (VK3). The hypothesis of VK3 mediating electron transfer between succinate:quinine oxidoreductase, from B. subtilis 168, and graphene oxide (GO) was proposed. BRGO was characterized by Raman spectroscopy, XPS and XRD and showed excellent properties. Furthermore, BRGO illustrates greater biocompatibility, with less toxicity, than GO and chemically reduced graphene oxide (CRGO) by zebrafish toxicity assessment, which demonstrates its great potential in various biomedical applications. © The Royal Society of Chemistry 2015.


Song T.-S.,Nanjing University of Technology | Song T.-S.,Jiangsu Branch of China Academy of Science and Technology Development | Jin Y.,Nanjing University of Technology | Bao J.,Nanjing University of Technology | And 5 more authors.
Journal of Hazardous Materials | Year: 2016

In this study, a simple method of biocathode fabrication in a Cr(VI)-reducing microbial fuel cell (MFC) is demonstrated. A self-assembling graphene was decorated onto the biocathode microbially, constructing a graphene/biofilm, in situ. The maximum power density of the MFC with a graphene biocathode is 5.7 times that of the MFC with a graphite felt biocathode. Cr(VI) reduction was also enhanced, resulting in 100% removal of Cr(VI) within 48 h, at 40 mg/L Cr(VI), compared with only 58.3% removal of Cr(VI) in the MFC with a graphite felt biocathode. Cyclic voltammogram analyses showed that the graphene biocathode had faster electron transfer kinetics than the graphite felt version. Energy dispersive spectrometer (EDS) and X-ray photoelectron spectra (XPS) analysis revealed a possible adsorption-reduction mechanism for Cr(VI) reduction via the graphene biocathode. This study attempts to improve the efficiency of the biocathode in the Cr(VI)-reducing MFC, and provides a useful candidate method for the treatment of Cr(VI) contaminated wastewater, under neutral conditions. © 2016 .


Zhu Z.,State Key Laboratory of Materials Oriented Chemical Engineering | Zhu Z.,Nanjing University of Technology | Guo T.,State Key Laboratory of Materials Oriented Chemical Engineering | Guo T.,National Engineering Technique Research Center for Biotechnology | And 12 more authors.
Journal of Biotechnology | Year: 2015

Clostridium scatologenes ATCC 25775T is an acetogenic anaerobic bacteria known to be capable of synthesizing volatile fatty acids and solvents from CO2 or CO on its autotrophic mode and producing 3-methylindole and 4-methylphenol on its heterotrophic mode. Here, we report the complete genome sequence of this strain, which might provide a lot of valuable information for developing metabolic engineering strategies to produce biofuels or chemicals from greenhouse gases. © 2015.


Zhang H.,Nanjing University of Technology | Zhu D.,Nanjing University of Technology | Song T.-S.,Nanjing University of Technology | Song T.-S.,Jiangsu Branch of China Academy of Science and Technology Development | And 5 more authors.
International Journal of Hydrogen Energy | Year: 2015

In this study, we demonstrate the effect of iron sheet on the output power of sediment microbial fuel cells (SMFCs). An SMFC with iron sheet present, but not in the circuit (SMFC-GF-iron) displayed a maximum power density of 63 mW m-2, whereas we find 37 mW m-2 for that SMFC with the iron sheet not present (SMFC-GF). Furthermore, the SMFC with an iron sheet in the circuit (SMFC-iron) had a maximum power density of 170 mW m-2. The effect of sheet iron, out of the circuit, was to improve the iron reduction microbial activity, while, within the circuit, it produced a large number of electrons from the electrochemical corrosion yielding higher power production. The study suggests that the addition of iron sheet to an SMFC is an easy and effective method for enhancing the output power of SMFCs. © 2015 Hydrogen Energy Publications, LLC.

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