Jiangsu Animal Husbandry and Veterinary College

Taizhou, China

Jiangsu Animal Husbandry and Veterinary College

Taizhou, China
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Zhang J.,Jiangnan University | Xia W.,Jiangnan University | Liu P.,Jiangsu Animal Husbandry and Veterinary College | Cheng Q.,Jiangnan University | And 3 more authors.
Marine Drugs | Year: 2010

Chitosan has received much attention as a functional biopolymer for diverse applications, especially in pharmaceutics and medicine. Our recent efforts focused on the chemical and biological modification of chitosan in order to increase its solubility in aqueous solutions and absorbability in the in vivo system, thus for a better use of chitosan. This review summarizes chitosan modification and its pharmaceutical/biomedical applications based on our achievements as well as the domestic and overseas developments: (1) enzymatic preparation of low molecular weight chitosans/chitooligosaccharides with their hypocholesterolemic and immuno-modulating effects; (2) the effects of chitin, chitosan and their derivatives on blood hemostasis; and (3) synthesis of a non-toxic ion ligand - D-Glucosaminic acid from Oxidation of D-Glucosamine for cancer and diabetes therapy. © 2010 by the authors.

Zhao J.K.,CAS National Astronomical Observatories | Zhao J.K.,Florida Institute of Technology | Oswalt T.D.,Florida Institute of Technology | Zhao G.,CAS National Astronomical Observatories | And 3 more authors.
Astronomical Journal | Year: 2013

We present chromospheric activity index S HK measurements for over 13,000 F, G, and K disk stars with high signal-to-noise ratio (>60) spectra in the Sloan Digital Sky Survey (SDSS) Data Release 7 (DR7) spectroscopic sample. A parameter δS is defined as the difference between S HK and a "zero" emission line fitted by several of the most inactive stars. The S HK indices of subgiant stars tend to be much lower than dwarfs, which provide a way to distinguish dwarfs and giants with relatively low-resolution spectra. Cooler stars are generally more active and display a larger scatter than hotter stars. Stars associated with the thick disk are in general less active than those of the thin disk. The fraction of K dwarfs that are active drops with vertical distance from the Galactic plane. Metallicity affects S HK measurements differently among F, G, and K dwarfs in this sample. Using the open clusters NGC 2420, M67, and NGC 6791 as calibrations, ages of most field stars in this SDSS sample range from 3 to 8 Gyr. © 2013. The American Astronomical Society. All rights reserved..

Zhang H.,Jiangnan University | Zhang H.,Jiangsu Animal Husbandry and Veterinary College | Jin Z.,Jiangnan University
Carbohydrate Polymers | Year: 2011

We prepared resistant starch (RS) from maize starch using a method combining α-amylase and pullulanase. We optimized reaction conditions for α-amylase, including temperature (T), pH, reaction time (t), and amount of α-amylase. The highest formation of RS (58.87%) was obtained under the following conditions: temperature, 90°C; pH, 5.5; time, 15 min; and amount of α-amylase, 4 μ/g. Scanning electron microscopy and differential scanning calorimetric analyses showed that maize starch underwent pasting treatment with α-amylase contained shorter amylase chains, and decreased steric hindrance among molecules compared with native maize starch. It is advantageous for amylopectin to be debranched by pullulanase, and the short amylose chains released from amylopectin can form double helices. Compared with native maize starch, maize RS showed increased crystallinity, and a larger, more compact laminiplantation structure. The increased density of the crystals greatly increases their resistance to starch-degrading enzymes. This is a promising method for preparing RS-rich products. © 2011 Elsevier Ltd. All rights reserved.

Xia W.,Jiangnan University | Liu P.,Jiangsu Animal Husbandry and Veterinary College | Zhang J.,Jiangnan University | Chen J.,Jiangnan University
Food Hydrocolloids | Year: 2011

Chitosan and its oligosaccharides, which are known to possess multiple functional properties, have attracted considerable interest due to their biological activities and potential applications in the food, pharmaceutical, agricultural and environmental industries. Many researchers have focused on chitosan as a potential source of bioactive materials in the past few decades. This review focuses on the biological activities of chitosan and chitooligosaccharides based on our and others' latest research results, including hypocholesterolemic, antimicrobial, immunostimulating, antitumor and anticancer effects, accelerating calcium and iron absorption, anti-inflammatory, antioxidant and Angiotensin-I-converting enzyme (ACE) inhibitory activities and so on, which are all correlated with their structures and physicochemical properties. The bioactivities summarized here may provide novel insights into the functions of chitosan, its derivatives or oligosaccharides and potentially enable their use as functional-food components and additives. © 2010 Elsevier Ltd.

Li P.,Nanjing Agricultural University | Han H.,State Key Laboratory of Crop Genetics and Germplasm Enhancement | Zhai X.,Jiangsu Animal Husbandry and Veterinary College | He W.,Jiangsu Animal Husbandry and Veterinary College | And 2 more authors.
Journal of Chromatographic Science | Year: 2012

An isocratic reversed-phase high-performance liquid chromatography method with UV detection is developed and validated for the simultaneous determination of ketamine, xylazine, and midazolam in canine plasma. Analytes are extracted from alkalinized samples into diethyl ethermethylene chloride (7:3, v:v) using single-step liquidliquid extraction. Chromatographic separation is performed on a C18 column using a mobile phase containing an acetonitrilemethanol- 10 mM sodium heptanesulfonate buffer adjusted to pH 3, with glacial acetic acid (44:10:46, v:v) at a detection wavelength of 210 nm, with a total runtime of 10 min. The calibration is linear over the range of 78.125-5000 ng/mL for ketamine and 15.625-1000 ng/mL for xylazine and midazolam. The limits of detection are 17.8, 10.3, and 15.1 ng/mL for ketamine, xylazine, and midazolam, respectively. The extraction recoveries are 76.1 for ketamine, 91.0 for midazolam, and 78.2 for xylazine. The method is successfully used for clinical and pharmacokinetic studies of the three-drug fixed dose combination formulations. © 2012 The Author.

Wang Z.-W.,Shanxi Agricultural University | Sun N.,Shanxi Agricultural University | Wu C.-H.,Jiangsu Animal Husbandry and Veterinary College | Jiang J.-B.,Shanxi Agricultural University | And 2 more authors.
Antiviral Therapy | Year: 2013

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) has caused large economic losses in the swine industry. Currently, there is no effective way to prevent PRRSV infection. In this study, we investigated the inhibitory effect of dipotassium glycyrrhetate (DG), a derivative of glycyrrhetinic acid, on PRRSV infection ability. Methods: The cytotoxicity of DG was measured by MTT assay, and the effects of DG on PRRSV N gene/protein were investigated using real-time PCR, western blot and immunofluorescence assay. In addition, the effect of DG on cell apoptosis was analysed by fluorescence staining. Results: Our results indicated that DG could effectively inhibit virus replication and N gene expression in MARC-145 cells infected with PRRSV. When the infected cells received DG, the numbers of apoptotic cells were decreased, and the cleaved caspase-3 contents were decreased dramatically. Conclusions: Our study demonstrates that DG could effectively inhibit the PRRS virus via multiple pathways including inhibition of virus replication and N gene expression and reduction of apoptotic cells. DG can serve as a potential chemical for PRRSV prevention and control. © 2013 International Medical Press.

Li S.,Yangzhou University | Li S.,Jiangsu Animal Husbandry and Veterinary College | Zhu C.,Yangzhou University | Tang L.,Yangzhou University | Kan J.,Yangzhou University
Materials Chemistry and Physics | Year: 2010

Fe-contained polyaniline (abbreviated as PANI-Fe) was prepared by chemical oxidation of aniline with ammonium peroxydisulfate oxidant in 0.5 mol dm -3 HCl and an adequate content of FeCl3·6H 2O solution in the presence of an applied magnetic field at room temperature. The X-ray photoelectron spectroscopy (XPS) and UV-vis and FTIR spectra suggest that there is an interaction between FeCl3 and PANI chains, but PANI-Fe backbone is essentially identical with that of parent polyaniline. The electron paramagnetic resonance (EPR) spectrum shows that there were unpaired electrons in PANI-Fe synthesized in the presence of an applied magnetic field, the spin density and the conductivity of which are 7.308 × 1020 spins g-1 and 0.891 S cm-1, respectively. The plot of magnetization (M) vs. the applied magnetic field (H) displays that the PANI-Fe possesses soft ferromagnetic behavior at room temperature. The results of cyclic voltammogram show that the PANI-Fe film is of excellent electrochemical activity. © 2010 Elsevier B.V. All rights reserved.

Chen H.,Yangzhou University | Li S.,Jiangsu Animal Husbandry and Veterinary College | Wang S.,Yangzhou University | Tan Y.,Yangzhou University | Kan J.,Yangzhou University
International Journal of Polymeric Materials and Polymeric Biomaterials | Year: 2013

A highly stable and effective catechol biosensor was prepared by immobilizing polyphenol oxidase (PPO) into polyaniline (PANI) film by using the direct electropolymerization (one-step) process in conjunction with cross-linking with glutaraldehyde. The results of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) confirmed that the immobilization of PPO was successful. The biosensor has a fast response to catechol (10 s) with a linear range of 1.0-100 μM and a detection limit of 0.01 μM. The maximum response current (Imax) and the Michaelis-Menten constant (k' m) were found to be 7.94 μA and 81.32 μM, respectively. The activation energy (E a) of the PPO catalytic reaction was 16.5 kJ/mol. The biosensor exhibited good stability by retaining 80% of its original activity when stored in a dry state at 4°C for up to 5 months. © 2013 Taylor & Francis Group, LLC.

Tan Y.,Yangzhou University | Kan J.,Yangzhou University | Li S.,Jiangsu Animal Husbandry and Veterinary College
Sensors and Actuators, B: Chemical | Year: 2011

A novel amperometric biosensor for the determination of catechol was developed accordingly to the electrochemical template procedure. The optimum fabricating conditions of the biosensor were studied. The resulting biosensor with the limit of less than 0.05 μM can be used for detection of catechol in the linear range of 2.5-140 μM. The maximum response current (I max) and the Michaelis-Menten constant (k′m) are 3.08 μA and 77.52 μM, respectively. The activation energy (Ea) of the polyphenol oxidase (PPO) catalytic reaction is 25.56 kJ mol-1 in the B-R buffer. The stability of the PANI-CA biosensor fabricated with the electrochemical template process (retains 86% of the original activity after four months) is much higher than that fabricated with one-step and two-step processes (retains 75% of the original activity after four months). The effects of potential and pH on the response current of the biosensor are also described. © 2010 Elsevier B.V. All rights reserved.

Xiong L.-W.,Shanghai Ocean University | Xiong L.-W.,Jiangsu Animal Husbandry and Veterinary College | Wang Q.,East China Normal University | Qiu G.-F.,Shanghai Ocean University
International Journal of Molecular Sciences | Year: 2012

Microsatellites are simple sequence repeats with a high degree of polymorphism in the genome; they are used as DNA markers in many molecular genetic studies. Using traditional methods such as the magnetic beads enrichment method, only a few microsatellite markers have been isolated from the Chinese mitten crab Eriocheir sinensis, as the crab genome sequence information is unavailable. Here, we have identified a large number of microsatellites from the Chinese mitten crab by taking advantage of Solexa genomic surveying. A total of 141,737 SSR (simple sequence repeats) motifs were identified via analysis of 883 Mb of the crab genomic DNA information, including mono-, di-, tri-, tetra-, penta- and hexa-nucleotide repeat motifs. The number of di-nucleotide repeat motifs was 82,979, making this the most abundant type of repeat motif (58.54%); the second most abundant were the tri-nucleotide repeats (42,657, 30.11%). Among di-nucleotide repeats, the most frequent repeats were AC motifs, accounting for 67.55% of the total number. AGG motifs were the most frequent (59.32%) of the tri-nucleotide motifs. A total of 15,125 microsatellite loci had a flanking sequence suitable for setting the primer of a polymerase chain reaction (PCR). To verify the identified SSRs, a subset of 100 primer pairs was randomly selected for PCR. Eighty two primer sets (82%) produced strong PCR products matching expected sizes, and 78% were polymorphic. In an analysis of 30 wild individuals from the Yangtze River with 20 primer sets, the number of alleles per locus ranged from 2-14 and the mean allelic richness was 7.4. No linkage disequilibrium was found between any pair of loci, indicating that the markers were independent. The Hardy-Weinberg equilibrium test showed significant deviation in four of the 20 microsatellite loci after sequential Bonferroni corrections. This method is cost- and time-effective in comparison to traditional approaches for the isolation of microsatellites. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

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