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Li Y.,Jiangsu Agri Animal Husbandry Vocational College | Li Y.,Yangzhou University | Bu S.,Yangzhou University
Journal of Veterinary Medical Science | Year: 2015

Diaveridine (DVD) is used in combination with sulphachloropyrazine (SPZ) as an effective antibacterial agent and antiprotozoal agent, respectively, in humans and animals. To gain a better understanding of the metabolism of SPZ and DVD in the food-producing animals, a high performance liquid chromatography (HPLC) method to determine and quantify sulphachloropyrazine (SPZ) and diaveridine (DVD) suspension residues from broilers is reported. Thirty healthy chickens were orally administered with sulphachloropyrazine-diaveridine (SPZ-DVD) suspension in water of 300 mg/l (SPZ) per day for seven successive days. Six chickens per day were slaughtered at 0, 1, 3, 5 and 7 days after the last administration. This procedure permitted SPZ and DVD to be separated from muscle tissue, liver, kidneys and skin with fat after extraction with acetonitrile and acetone under slightly acidic conditions. From the detected residuals in different tissues, we found that SPZ was quickly eliminated in liver and muscle, and slowly eliminated in kidney and skin with fat. DVD was quickly eliminated in liver and slowly eliminated in kidney. The withdrawal period of SPZ was 3.26, 3.72, 4.39 and 5.43 days in muscle, liver, kidney and skin with fat, respectively. The withdrawal period of DVD was 4.77, 4.94, 6.74 and 4.58 days in muscle, liver, kidney and skin with fat, respectively. Therefore, the suggested withdrawal period for SPZ-DVD suspension should be 7 days after dosing for seven successive days. © 2015 The Japanese Society of Veterinary Science.

Yuan S.,CAS Wuhan Institute of Hydrobiology | Yuan S.,Jiangsu Agri Animal Husbandry Vocational College | Xi B.W.,Chinese Academy of Fishery Sciences | Wang J.G.,CAS Wuhan Institute of Hydrobiology | And 2 more authors.
Parasitology Research | Year: 2015

We describe here a new myxozoan, Thelohanellus wangi n. sp., infecting the allogynogenetic gibel carp, Carassius auratus gibelio (Bloch), in a fry nursery farm in Jiangsu Province, China. Polysporous gray white round or ovoid plasmodia, 500–1,500 μm in size, were found exclusively in the gill filaments. The diagnostic characteristics of the myxospores are as follows: spore melon seed shaped in frontal view with smooth surface and asymmetrical valves; convex-shaped in sutural view with straight or slightly bent and thick sutural line, averaging 20.2 (16.5–22.3) × 9.9 (9.1–10.8) × 9.3 μm (8.2–10.4) in size; and one elliptical polar capsules with subulate anterior end and round posterior end, averaging 10.1 (8.4–11.2) × 6.5 μm (6.1–7.0) in size. Polar filaments coil six to seven turns and are slightly oblique to the longitudinal axis of the polar capsule. An elongate melon seed-shaped mucus envelope surrounds almost completely the spore, but with largest distance in posterior end between spore valve and mucus envelope. Granular sporoplasm contains two slightly oval nuclei, and no iodinophilous vacuole is observed. Histopathological analysis showed that the plasmodia embedded in the gill filaments and occasionally extended into the connective tissue of the gill arch. No significant inflammatory responses were provoked by the infection. The development of parasite is asynchronous, with mature spores centrally locating and trophozoites and pre-sporogonic stages peripherally locating within the plasmodia. Overall prevalence was 74 % (37/50). Infection intensity was usually high, with about 10–30 cysts per fish. Phylogenetical analysis shows that T. wangi n. sp. is most closely related to several Thelohanellus species infecting cyprinid fish and formed a Thelohanellus clade based on the ribosomal DNA data. The cysts of this myxosporean were only found from fry seed with body length below 7 cm, generally from late April to early June when the fry fish of allogynogenetic gibel carp were nursed in the epizootic areas. © 2014, Springer-Verlag Berlin Heidelberg.

Fang X.,Jiangsu Agri Animal Husbandry Vocational College
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2013

To study the generation and structure of anti-HBV IgY in eggs from hens immunized with recombinant yeast HBV vaccine. From the hens immunized three times with the recombinant yeast HBV vaccine, the eggs were collected to extract anti-HBV IgY. After purified, the anti- HBV IgY was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reverse phase high-performance liquid chromatography (RP-HPLC), and its secondary structure was examined by circular dichroism (CD) chromatography. The relative molecular mass (Mr;) of anti-HBV IgY expressed by the recombinant yeast HBV vaccine on hens was 178 000. CD chromatography showed that the α-helix, β-fold, β-turn and random coil of anti-HBV IgY had 38, 0, 44 and 17 amino acids, respectively. The anti-HBV IgY can be expressed in eggs from hens immunized with recombinant yeast HBV vaccine. The α-helix and β-turn are major secondary structures of anti-HBV IgY.

Wang Q.,Jiangsu Agri Animal Husbandry Vocational College | Chen X.,Jiangsu Agri Animal Husbandry Vocational College
Advance Journal of Food Science and Technology | Year: 2015

Aim of study on biological characteristics and propagative technologies of Rgodeous sinensis is of great significance to rationally utilizing its resource, protecting the species, improving the benefit of aquaculture. Research contents comprise biological characteristics, living habits, propagative habits, the source of its parent fish, rearing of parent fish and spawning via artificial estrus and incubation. It is discovered that the main point for propagating Rhodeus sinensis lies in identification of the parent fish of Rhodeus sinensis, appropriate proportion among males and females, employment of odinagogue, appropriate selection of categories and specifications of freshwater mussels. © Maxwell Scientific Organization, 2015.

Li F.,Nanjing University | Li F.,Jiangsu Agri Animal Husbandry Vocational College | Duan J.-A.,Nanjing University | Qian D.,Nanjing University | And 7 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2013

A rapid and sensitive hydrophilic interaction liquid chromatography method was developed for the identification and quantitative determination of 17 nucleosides and nucleobases by using ultra-high performance liquid chromatography separation system coupled to triple quadrupole mass spectrometer. Sufficient separation of the target compounds was achieved on an Acquity BEH Amide column (2.1mm×100mm, 1.7μm) in a single run of 9min. The LOD and LOQ were in the range of 0.017-50.00ngml-1 and 0.056-146.00ngml-1, respectively. Then, the method was applied for the analysis of nucleosides and nucleobases in Elaphuri Davidiani Cornu and Cervi Cornu. Multivariate statistical analysis was also used to investigate the differences in the nucleosides and nucleobases profiles among the samples. The present study also provided helpful information for quality control of Elaphuri Davidiani Cornu and Cervi Cornu. © 2013 Elsevier B.V.

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