Jiangnan University , JU, literally "Southern Yangtze University") is a university located in Wuxi, Jiangsu, China. It is a key national university of the “Project 211” and is directly administrated by the Chinese Ministry of Education. The distinguished disciplines are food science and technology, fermentation engineering and industrial design. Wikipedia.
Jiangnan University | Date: 2016-07-08
The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineered B. subtilis Deletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineered B. subtilis for GlcNAc production, which can be further applied to industrial production of GlcNAc.
CAS Institute of Microelectronics and Jiangnan University | Date: 2016-06-06
A method for preparing a TiAl alloy thin film, wherein a reaction chamber is provided, in which at least one substrate is placed; an aluminum precursor and a titanium precursor are introduced into the reaction chamber, wherein the aluminum precursor has a molecular structure of a structural formula (I); and the aluminum precursor and the titanium precursor are brought into contact with the substrate so that a titanium-aluminum alloy thin film is formed on the surface of the substrate by vapor deposition. The method solves the problem of poor step coverage ability and the problem of incomplete filling with regard to the small-size devices by the conventional methods. Meanwhile, the formation of titanium-aluminum alloy thin films with the aid of plasma is avoided so that the substrate is not damaged by plasma.
Jiangnan University and Nanjing Jianggao Drying Equipment Co. | Date: 2016-04-04
A method for preserving and conditioning beef comprises: cleaning and chopping picked fresh beef, dipping in composite essential oil prepared from clove essential oil, cinnamon essential oil and illicium verum essential oil for 30 s and taking out and draining slightly; placing the beef dipped into a modified atmosphere packaging box and fast precooling; inflating gas of mixed proportion into the cooled modified atmosphere packaging box with beef for preservation packaging and then storing at the low temperature of 0-4 C. The shelf life of the beef packaged by the method is up to 24 d. The aerobic bacterial count is not over 6.0 lgcfu/g. A total volatile basic nitrogen TVB-N value is not over 20 mg/100 g and belongs to the range of second freshness. The beef is stable in color, is cherry-red, has the advantage of good preservation effect and is used for cooking preserved cooked beef.
Jiangnan University, Guangdong Jiahao Food Co. and Nanjing Jianggao Drying Equipment Co. | Date: 2016-04-04
A method for enhancing sauce flavor including steps of: collection, dilution, denaturation, enzymolysis, enzyme deactivation, centrifugation, secondary enzymolysis, deodorization, Maillard reaction, seasoning, dosing and flexible sterilization of a raw material, salted duck egg white. The by-product, salted duck egg white, not fully used in the food processing industry is recycled as a raw material, which not only saves the cost but also can protect the environment. Hydrolyzed animal protein is obtained through double-enzyme fractional hydrolysis. The degree of hydrolysis is high. The obtained hydrolyzed protein contains no trichloropropanol and is relatively safe, and flavourzyme decomposes bitter peptide, to make the hydrolysate not taste bitter. Some yeast, cooking wine and essence with unique flavor are chosen. Each ingredient is in a reasonable proportion, and the obtained product is delicious and of mellow flavor.
Jiangnan University | Date: 2015-03-26
An inductive magnetoelectric biochemical reaction system includes a reaction unit which includes a reaction chamber, which includes a reactant container and is disposed in a rotatable perpendicular magnetic field; a primary coil, which is wound around one side of a closed-loop iron core and is connected to a control unit; a secondary coil, which is wound around the other side of the closed-loop iron core and includes an insulating tube which allows a reaction solution acting as a conductor, two ends of the insulating tube being communicated with the reactant container; a rotating magnetic field unit, which is configured to generate the rotatable perpendicular magnetic field; and a control unit, which is at least configured to adjust an excitation voltage and a signal type applied on the primary coil.
Jiangnan University | Date: 2016-02-24
The present invention provides methods for deinking wastepaper by combined use of cutinase and chemical reagents, which relates to the field of enzyme engineering. The method comprises the following steps: pulp preparation, enzymatic hydrolysis, pulp washing and dewatering, and flotation. The enzyme for enzymatic hydrolysis is cutinase at a concentration of 10-20 U/g absolute dry pulp; and 0.5-4% Na_(2)SiO_(3), 0.1-0.8% MgSO_(4), 0.1-0.8% EDTA, 0.1-4% H_(2)O_(2 )are used in the chemical treatment. With combined use of enzymatic and chemical treatment, the present invention has solved the problem of the current enzymatic method for deinking that requires large dosage of enzymes and thus high cost. Through proper choice of the kind and the amount of chemical reagents, synergistic effects of the enzymatic and chemical treatment can be achieved, thus increasing the effectiveness of the deinking process. In addition, this method does not require alkaline chemicals, which alleviates the problem of sewage treatment associated with conventional deinking methods.
Jiangnan University | Date: 2016-06-22
The present invention provides a mutant with enhanced secretion of L-asparaginase and its application, which relates to the field of enzyme engineering. This invention successfully constructed a mutant with enhanced secretion of L-asparaginase through N-terminal deletion on amino acid sequence of L-asparaginase, and then expressed the mutant via a recombinant strain. Compared with the wild L-asparaginase, the secretion ability of L-asparaginase mutant in present invention significantly improves by 3.14 times compared to that of wild type. The recombinant strain that constructed in this prevention has an L-asparaginase yield of 407.6 U/mL and a production efficiency of 9.26 U/(mL/h), which reaches to the highest yield been reported.
Jiangnan University | Date: 2016-11-11
The invention discloses a promoter which can be induced to express in acidic conditions, and relates to the field of bioengineering technology. The promoters of the invention are separated from A. niger and can actuate and/or regulate the expression of the effectively connected nucleic acids in A. niger. In the invention the expression of the promoters is studied in A. niger, and it is indicated that some promoters show weak expression, and some show strong activity. The invention provides an effective method and new thought for organic acids production by fungi or other products produced by fermentation under acidic conditions.
Jiangnan University | Date: 2016-09-19
The present invention provides a method for vegetable oil deacidification by enzymatic amidation, which relates to the field of oil refining technology. The present invention is carried out through mixing high acid value vegetable oil with ethanolamine at a certain molar ratio in a solvent or solvent-free system, adding with a certain amount of lipase, and reacting at certain temperature for a period of time. The monoethanolamine has been used as an acyl donor for the first time to react with free fatty acid, which avoids the increasing amount of by-products and great loss of neutral oil in reaction that involved with triglycerides. The method of the present invention has the advantages of high selectivity, high catalytic efficiency, and environment friendly in the reaction. From enzymes recycling, it greatly reduces costs, which shows tremendous potential in the application.
Jiangnan University | Date: 2016-11-09
The present invention provides a method for scarless in vitro DNA assembly using thermostable exonucleases and ligase, which relates to the field of genetic engineering. The present invention provides a fast method for assembling DNA subfragments with homologous ends, which employs thermostable polymerases and ligase in a thermal cycle of denaturation, annealing, digestion and ligation. After denaturation, DNA subfragments are assembled together via annealing of the homologous end sequences, the unpaired single-stranded overhangs are digested by polymerases, and the resulting nicked gaps are sealed by a ligase. Using this method, 2-6 DNA subfragments were successfully assembled within two hours. This method can be used in conventional DNA recombination and be adapted to high throughput assembly operations. In addition, combinatorial mutations can be easily introduced into the assembled sequence by use of primers with mutated bases. It is particularly suitable for making enzyme and synthetic pathways mutation libraries with high diversity, which can be used in directed evolution to screen for enzymes and synthetic pathways with desirable properties.