Wuxi, China
Wuxi, China

Jiangnan University , JU, literally "Southern Yangtze University") is a university located in Wuxi, Jiangsu, China. It is a key national university of the “Project 211” and is directly administrated by the Chinese Ministry of Education. The distinguished disciplines are food science and technology, fermentation engineering and industrial design. Wikipedia.


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The invention discloses a method and device of dynamically configuring linear density and blending ratio of yarn by four-ingredient asynchronous/synchronous drafted, comprising: a drafting and twisting system, which includes a first stage drafting unit, a successive second stage drafting unit and an integrating and twisting unit. The first stage drafting unit includes a combination of back rollers and a middle roller. The second stage drafting unit includes a front roller and the middle roller. Blending proportion and linear densities of the four ingredients are dynamically adjusted by the first stage asynchronous drafting mechanism, and reference linear density is adjusted by the second stage synchronous drafting mechanism. The invention can not only accurately control a linear density change, but also accurately control color change of the yarn. Further, rotation rate of the middle roller is constant, ensuring a reproducibility of patterns and colors of the yarn with changing linear density.


The invention discloses a method of dynamically configuring linear density and blending ratio of yarn by three-ingredient asynchronous/synchronous drafted, comprising: a drafting and twisting system, which includes a first stage drafting unit, a successive second stage drafting unit and an integrating and twisting unit. The first stage drafting unit includes a combination of back rollers and a middle roller. The second stage drafting unit includes a front roller and the middle roller. Blending proportion and linear densities of three ingredients are dynamically adjusted by the first stage asynchronous drafting mechanism, and reference linear density is adjusted by the second stage synchronous drafting mechanism. The invention can not only accurately control linear density change, but also accurately control a color change of the yarn. Further, the rotation rate of the middle roller is constant, ensuring a reproducibility of the patterns and colors of the yarn with a changing linear density.


The present invention provides a novel method that recovers metal components from sulfide mineral tailings by Microbial Fuel Cell. The traditional bio-hydrometallurgy reaction is split to one oxidization reaction taking place in the anode chamber and one reduction reaction taking place in the cathode chamber. H^(+ )generated during the oxidization reaction is continually transferred to cathode chamber through proton exchange membrane and reacted with O_(2 )to generate H_(2)O, which not only increases the reaction rate in anode chamber but also decreases equipment corrosion caused by excessive H^(+). The method of the present invention, recovering metals as well as electronic power, is environment-friendly.


The invention provides a fuzzy curve analysis based soft sensor modeling method using time difference Gaussian process regression, it is suitable for application in chemical process with time delay characteristics. This method can extract stable delay information from the historical database of process and introduce more relevant modeling data sequence to the dominant variable sequence. First of all, the method of fuzzy curve analysis (FCA) can intuitively judge the importance of the input sequence to the output sequence, estimate the time-delay parameters of process, and such offline time-delay parameter set can be utilized to restructure the modeling data. For the new input data, based on the historical variable value before a certain time, the current dominant value can be predicted by time difference Gaussian Process Regression (TDGPR) model. This method does not encounter the problem of model updating and can effectively track the drift between input and output data. Compared with steady-state modeling methods, this invention can achieve more accurate predictions of the key variable, thus improving product quality and reducing production costs.


The invention discloses a method and device of dynamically configuring a linear density and a blending ratio of a yarn by five-ingredient asynchronous/synchronous drafting, comprising: a drafting and twisting system, which includes a first stage drafting unit, a successive second stage drafting unit and an integrating and twisting unit. The first stage drafting unit includes a combination of back rollers and a middle roller. The second stage drafting unit includes a front roller and the middle roller. The blending proportion and linear densities of the five ingredients are dynamically adjusted by the first stage asynchronous drafting mechanism, and the reference linear density is adjusted by the second stage synchronous drafting mechanism. The invention can not only accurately control a linear density, but also accurately control a color change of the yarn.


The present invention relates to the field of biotechnology engineering. It provides a method of constructing a recombinant Bacillus subtilis that can produce specific-molecular-weight hyaluronic acids. By integranted expression of hasA from Streptococcus zooepidemicus and overexpression of genes of HA synthetic pathway, tuaD, glmU and glmS, high yield HA production was achieved in the recombinant strain. Additionally, introduction and functional expression of the leech hyaluronidase in the recombinant strain substantially increased the yield of HA to 19.38 gL^(1). Moreover, HAs with a broad range of molecular weights (10^(3 )Da to 10^(6 )MDa) were efficiently produced by controlling the expression level of hyaluronidase using RBS mutants with different translational strengths. The method of the present invention can be used to produce low molecular weight HAs at large scale in industrial applications.


Zhao Y.,Jiangnan University | Xu L.,Jiangnan University | Kuang H.,Jiangnan University | Wang L.,Jiangnan University | Xu C.,Jiangnan University
Journal of Materials Chemistry | Year: 2012

Scalable preparation of nanoparticle assemblies and superstructures with control over the size, composition, and geometry represents a challenge for many practical applications of nanomaterials. DNA nanotechnology offers both versatility of particle arrangements and multiple gateways to interesting optical, electronic, biological, and catalytic properties. In this work we used a polymerase chain reaction (PCR) to produce complex superstructures from heterogeneously-sized gold nanoparticles. By controlling the number of PCR cycles and primer density, two distinct superstructures were assembled at asymmetric and symmetric PCR. With the increasing of PCR number, the size of structures was accordingly increased in the range 35-600nm. More importantly, the scaled-up GNP superstructures exhibited tunable chirality, which showed distinct differences between asymmetric and symmetric PCR. The origin of the chirality was analyzed from both an experimental and theoretical point of view. The heterogeneity of GNPs and conformational transition of self-assembly contributed to the occurrence of chirality in the GNP superstructures. PCR could be a very important and potentially useful tool for the controllable preparation of scaled-up superstructures with tunable chirality, and this experimental and mechanical research could significantly promote the technical development of chiral material preparation. © The Royal Society of Chemistry 2012.


Ding S.,Jiangnan University | Ding R.,Jiangnan University | Yang E.,University of Stirling
Journal of the Franklin Institute | Year: 2014

This paper uses the filtering technique, transforms a pseudo-linear auto-regressive system into an identification model and presents a new recursive least squares parameter estimation algorithm pseudo-linear auto-regressive systems. The proposed algorithm has a high computational efficiency because the dimensions of its covariance matrices become small compared with the recursive generalized least squares algorithm. © 2013 The Franklin Institute Published by Elsevier Ltd. All right sreserved.


Liu P.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
International Journal of Food Science and Technology | Year: 2012

Purple-fleshed sweet potato (PFSP) with high anthocyanin level was attractive to health concern. Three drying methods, namely microwave-assisted freeze-drying (MWFD), microwave-assisted vacuum drying (MWVD) and microwave-assisted spouted bed drying (MWSBD), were compared in the drying of PFSP granules. Product's texture, colour, anthocyanin level and energy consumption were investigated. Drying periods varied among drying methods. MWSBD needed the least time to finish the drying process. Different drying methods had a significant effect (P<0.05) on texture and anthocyanin level. Maximum penetration force of MWSBD treated sample was lowest (14.99N). Anthocyanin level of MWSBD products was not as high as that of MWFD products, but colour and appearance were fine. MWFD products owned good crispness through sensory evaluation. Energy consumption of MWFD was about two times higher than that of MWVD. Final results indicated that MWSBD may be an alternative way to MWFD with measurements to maintain the anthocyanin level. © 2011 The Authors. International Journal of Food Science and Technology © 2011 Institute of Food Science and Technology.


Ding J.,Nanjing University of Posts and Telecommunications | Ding J.,Jiangnan University | Ding F.,Jiangnan University
International Journal of Adaptive Control and Signal Processing | Year: 2011

Identification problems of output error models with moving average noises are considered in this paper. The least-squares-based parameter estimation is biased under the colored noises in outputs. Firstly, a bias compensation term is formulated to achieve the bias-eliminated estimates of the system parameters. Secondly, the bias compensation term is determined by the unknown variance of the noise and the unknown noise model, thus based on the hierarchical identification principle, an unbiased parameter estimation is obtained by interactively estimating noise variance and noise parameters. Finally, the estimated bias compensation term is added to the biased parameter estimates. The simulation examples confirm the effectiveness of the proposed algorithm. Copyright © 2011 John Wiley & Sons, Ltd.


Zhu Y.,Jiangnan University | Liu X.,Jiangnan University | Gao J.,Jiangnan University | Zhang Y.,Jiangnan University | Zhao F.,Beijing Normal University
Optics Express | Year: 2014

We develop a novel model of the probability density of the orbital angular momentum (OAM) modes for Hankel-Bessel beams in paraxial turbulence channel based on the Rytov approximation. The results show that there are multi-peaks of the mode probability density along the radial direction. The peak position of the mode probability density moves to beam center with the increasing of non-Kolmogorov turbulence-parameters and the generalized refractive-index structure parameters and with the decreasing of OAM quantum number, propagation distance and wavelength of the beams. Additionally, larger OAM quantum number and smaller non-Kolmogorov turbulence-parameter can be selected in order to obtain larger mode probability density. The probability density of the OAM mode crosstalk is increasing with the decreasing of the quantum number deviation and the wavelength. Because of the focusing properties of Hankel-Bessel beams in turbulence channel, compared with the Laguerre-Gaussian beams, Hankel-Bessel beams are a good light source for weakening turbulence spreading of the beams and mitigating the effects of turbulence on the probability density of the OAM mode. © 2014 Optical Society of America.


Sheng X.,Jiangnan University | Zhang Y.,Jiangnan University | Zhao F.,Beijing Normal University | Zhang L.,Jiangnan University | Zhu Y.,Jiangnan University
Optics Letters | Year: 2012

Based on Zernike-model expansion of turbulence phase aberrations and non-Kolmogorov spectrum model of indexof-refraction fluctuation, we analyze the effects of low-order Zernike turbulence aberrations on orbital angular momentum (OAM) entanglement states in a weak fluctuation region. The signal photon detection probability of OAM entanglement states propagating in a slant turbulence channel with non-Kolmogorov turbulence Z-tilt, defocus, astigmatism, and coma aberrations are modeled, respectively. The results demonstrate that turbulence Z-tilt aberration is the dominant aberration, coma is the second, and astigmatism is the third, but that the defocus aberration has no impact on the detection probability. As the power-law exponent of the non-Kolmogorov spectrum increases from 3 to 4, the detection probability decreases. © 2012 Optical Society of America.


Liang R.,Jiangnan University | Liang R.,University of California at Davis | Huang Q.,Rutgers University | Ma J.,Jiangnan University | And 2 more authors.
Food Hydrocolloids | Year: 2013

In order to overcome the limitations of liquid-base emulsion system, beta-carotene nanoemulsions stabilized by modified starch were spray-dried to powders after the emulsification process. The powders showed a good dissolution in water and the reconstituted emulsions had similar particle sizes with the fresh nanoemulsions. A 30 days storage test was carried out to investigate the effect of relative humility (RH) on the storage stability of beta-carotene powders at 25.0°C. The beta-carotene degradation profiles over time were found to fit well with a Weibull model and also closely related to the film property of the matrix, moisture sorption property and glass transition temperature of the powder. The results showed that modified starches with lower film oxygen permeability had a higher retention of beta-carotene during storage. The glass transition temperature of powder in different RH also affected the rate of beta-carotene degradation. Overall these results provide useful information for choosing wall materials and storage conditions to protect nutraceuticals in delivery systems. © 2013 Elsevier Ltd.


Yin X.,Jiangnan University | Madzak C.,French National Institute for Agricultural Research | Du G.,Jiangnan University | Zhou J.,Jiangnan University | Chen J.,Jiangnan University
Applied Microbiology and Biotechnology | Year: 2012

In previous research, a thiamine-auxotrophic yeast for alpha-ketoglutaric acid (KGA) overproduction was screened in our laboratory and named Yarrowia lipolytica WSH-Z06 (CCTCC no. M207143). However, the high concentration of by-products (mainly pyruvate) limited its application on an industrial scale. To enhance KGA production and reduce pyruvate (PA) accumulation, the pyruvate carboxylation pathway was regulated. By overexpressing the pyruvate carboxylase genes ScPYC1 from Saccharomyces cerevisiae and RoPYC2 from Rhizopus oryzae in Y. lipolytica WSH-Z06, the yields of KGA in Y. lipolytica-ScPYC1 and Y. lipolytica-RoPYC2 increased by 24.5 and 35.3 %, and the yields of PA decreased by 51.9 and 69.8 % in shake flasks, respectively. These changes in the expression levels and activities of key intracellular enzymes showed that enhancing the pyruvate carboxylation pathway had successfully redistributed the carbon flux from PA to KGA. Finally, by controlling the pH in a 3-L fermenter, the maximum concentration of KGA in Y. lipolytica-RoPYC2 reached 62.5 gL -1 with an evident decrease in PAyield from 35.2 to 13.5 gL -1. © Springer-Verlag Berlin Heidelberg 2012.


Wu J.,Jiangnan University | Zhong F.,Jiangnan University | Li Y.,Jiangnan University | Shoemaker C.F.,University of California at Davis | Xia W.,Jiangnan University
Food Hydrocolloids | Year: 2013

The characteristics of pullulan-chitosan and pullulan-carboxymethyl chitosan (CMCH) blended films were investigated. The viscosity of the film-forming solutions, mechanical properties, barrier properties (water and oxygen), water solubility, and color are reported. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) measurements were used to study the interactions and compatibility between the polysaccharides. The addition of the chitosan or CMCH to pullulan effectively modified the mechanical and oxygen barrier property of the film. The improvements in the film properties have been related to strong interactions between chitosan/CMCH and pullulan polymers, which were observed with FTIR spectroscopy. The ratio of pullulan and chitosan to achieve films with optimum mechanical properties, oxygen and water barrier properties was identified to be 1:1. Increased concentrations of chitosan lead to the formation of inter-molecular hydrogen bonds rather than the intra-molecular hydrogen bonds which were suggested from XRD measurements. © 2012 Elsevier Ltd.


Li Y.,Jiangnan University | Zhong F.,Jiangnan University | Ji W.,Jiangnan University | Yokoyama W.,U.S. Department of Agriculture | And 3 more authors.
Food Hydrocolloids | Year: 2013

The Maillard reaction was carried out under wet reaction conditions using rice protein product from limited hydrolysis to conjugate with various mono-, oligo- and polysaccharides. The Maillard reaction products (MRPs) prepared with rice protein hydrolysates at 5% degree (hydrolysed by Protease N) and dextran T20 (20 min at 100 °C) showed the greatest improvement in functionality. The solubility, emulsification activity (EA), and emulsification stability (ES) of the MRPs increased by factors of 3.5, 5.3 and 7.3 times, respectively as compared to MRPs formed with native rice proteins and dextran T20. Amino acid analysis indicated that lysine and arginine decreased significantly in the MRPs. Formation of MRPs reduced the surface hydrophobicity, which was in agreement with the change of solubility. However, further decreasing of the surface hydrophobicity resulted in lower EA and ES during the late stage of the reaction. Fluorescence analysis suggested that formation of late stage MRPs occurred after 20 min of the Maillard reaction. The molecular weight distributions showed that the functional properties of MRPs and the mechanisms of formation were affected by the peptide chain length. © 2012 Elsevier Ltd.


Wang R.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
Drying Technology | Year: 2010

In this study, the effects of osmotic pretreatment on microwave freeze-drying (MFD) characteristics and quality of potato chips were studied. Potato slices were pretreated by immersion in salt solution (5, 10, 15% w/w) or sucrose solution (30, 40, 50% w/w) at 20°C for 6 h prior to MFD. Results showed that osmotic pretreatment significantly improved the MFD drying rate compared with MFD of fresh samples under the same operating conditions. However, there is an optimal level of osmotic dehydration beyond which it has an adverse effect on product quality. Osmotic pretreatment with salt concentration below 5% and/or sucrose concentration below 30% prior to MFD drying resulted in dried products of good quality with shorter total drying times relative to MFD of untreated samples. © 2010 Taylor & Francis Group, LLC.


Jiang H.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
Drying Technology | Year: 2010

This experimental study investigated the drying characteristics of microwave freeze drying (MFD) of banana slices of varying maturity. Results show that the drying time was shortened with increase of both microwave power and degree of maturity of the banana. In terms of the quality parameters such as hardness, fracturability, color, and rehydration ratio, high maturity samples with high sugar content displayed better performance. The best sensory scores were obtained for medium maturity bananas processed using 2.0 W/g microwave power. © 2010 Taylor & Francis Group, LLC.


Guo X.,Jiangnan University | Huang Y.-G.,China Institute of Technology
Journal of Food and Drug Analysis | Year: 2015

Over recent years, an increase in alcohol-related problems has been noted in China. Taking effective measures against the problem requires clear reviewing and understanding of the evolution of the Chinese alcohol policy. This study is aimed to evaluate the alcohol policy with special focus on reviewing the alcohol production and consumption situation in China and assessing the changes in Chinese alcohol policy along with other related fields. This article finishes with a set of recommended policy changes that could help solve the recent alcohol-related problems and analyze the major impediments. © 2014, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. All rights reserved.


Wang Y.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore | Mothibe K.J.,Jiangnan University
Drying Technology | Year: 2012

Stem lettuces are an important vegetable due to its nutritional characteristics. Stem lettuce was used as the model in this investigation. The ice-melting and freezing point temperature as well as distribution and migration of unfrozen water in stem lettuce cubes during the microwave freeze drying (MFD) step were studied using differential scanning calorimetry (DSC) and low-field pulsed nuclear magnetic resonance (LF-NMR). MFD of stem lettuce cubes was carried out in three circular conduits with different diameters (40, 55, and 70 mm) at a microwave frequency of 2,450 MHz. The total drying time for MFD was almost the same for all circular conduits; that is, approximately 4.5 h. This was less than the 7.0 h required for conventional radiation freeze drying (FD) in a 40-mm-diameter circular conduit and far less than the 20 h required for FD in a flat slab model. The microwave energy supplied and specimen temperature profiles during MFD were strongly influenced by the tissues, unfreezable water content, moisture distribution, and ionic characteristics of the samples. It was also found that the MFD process involving the conduits can be divided into three steps based on the amount and distribution of unfrozen water in the frozen samples and how heat is supplied in order to prevent corona discharge. © 2012 Copyright Taylor and Francis Group, LLC.


Tang C.-D.,Key Laboratory of Industrial Biotechnology | Wang J.-Q.,Key Laboratory of Industrial Biotechnology | Wu M.-C.,Jiangnan University
Journal of Agricultural and Food Chemistry | Year: 2012

A cDNA fragment of the Anman5A, a gene that encodes an acidophilic β-mannanase of Aspergillus niger LW-1 (abbreviated as AnMan5A), was cloned and functionally expressed in Pichia pastoris. Homology alignment of amino acid sequences verified that the AnMan5A belongs to the glycoside hydrolase (GH) family 5. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay demonstrated that the recombinant AnMan5A (reAnMan5A), a N-glycosylated protein with an apparent molecular weight of 52.0 kDa, was secreted into the medium. The highest reAnMan5A activity expressed by one P. pastoris transformant, labeled as GSAnMan4-12, reached 29.0 units/mL. The purified reAnMan5A displayed the highest activity at pH 3.5 and 70 °C. It was stable at a pH range of 3.0-7.0 and at a temperature of 60 °C or below. Its activity was not significantly affected by an array of metal ions and ethylenediaminetetraacetic acid (EDTA). The Km and Vmax of the reAnMan5A, toward locust bean gum, were 1.10 mg/mL and 266.7 units/mg, respectively. © 2011 American Chemical Society.


Wang Y.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore | Mujumdar A.S.,Institute of Chemical Technology
Food Hydrocolloids | Year: 2013

Three types of gels including tapioca starch gel, fish gel and mixed gels of both (cassava starch: fish = 1:1) were prepared under different thermal conditions. Slices (4 mm thick) derived from these the gels were dehydrated to a final moisture content of 7% in a microwave vacuum dryer. The micro/macro structure and selected quality parameters of the dried gel chips were measured e.g. shape, relative volume, bulk density, texture, color, sensory and microstructure. The measured gel characteristics were analyzed and related to structure development and selected quality attributes. Both fish and mixed gel chips expanded in both diameter and thickness while the starch gel chips shrank in diameter, but expanded in thickness; the former two represented a continuous cross-section composed of a cellular structure and expanded uniformly in thickness while the latter developed an open cross-section and the center expanded more than the edge region, indicative of a disc and pillow shape, respectively. The higher severity of thermal treatment favored greater expansion and reduced hardness and bulk density, and increased crispness in the mixed gel chips; they developed a smooth and fine surface and pore wall but decreased lightness and whiteness due to starch gelatinization in the starch and mixed gel chips. Only the partial dried mixed gel chips are acceptable to the panelists. A homogenous co-gel in the mixed gel contributed to the higher uniform expansion and cellular structure. These findings will help developing the restructured microwave vacuum-dried product by blending the starch with the fish. © 2013 Elsevier Ltd.


Tang K.,Jiangxi Agricultural University | Gu X.,Jiangnan University | Luo Q.,Jiangxi Agricultural University | Chen S.,Jiangnan University | And 2 more authors.
Food Chemistry | Year: 2014

A high selective pre-treatment method for the analysis of sulphonylurea herbicides (SUHs) in rice grain samples based on molecularly imprinted solid-phase extraction (MISPE) was developed. The molecularly imprinted polymers (MIPs) were synthesised with high adsorption capacity and suitable particle size using pyrazosulphuron ethyl (PS) as the template and by screening three porogens and porogen volumes. The resulting MIPs showed high selectivity for PS. The MISPE procedure was developed for the purification and enrichment of PS, bensulphuron methyl (BSM), tribenuron methyl (TBM), metsulphuron methyl (MSM) and nicosulphuron (NS) from a rice grain sample prior to reversed-phase high-performance liquid chromatography (HPLC) analysis. Average recoveries of the PS, NS, MSM and BSM were 95.6%, 88.6%, 87.8% and 89.4%, respectively, but the recovery of TBM was lower (48.2%). This pre-treatment methodology for extracting SUHs was simple and cleaner extractions. The limits of detection ranged from 10.1 to 50.0 ng L-1. Five local rice grain samples were analysed by HPLC-MISPE, and PS was detected in all five samples, while BSM was detected in one sample. © 2013 Elsevier Ltd. All rights reserved.


Wang L.,Jiangnan University | Xu L.,Jiangnan University | Kuang H.,Jiangnan University | Xu C.,Jiangnan University | Kotov N.A.,University of Michigan
Accounts of Chemical Research | Year: 2012

Although nanoparticle (NP) assemblies are at the beginning of their development, their unique geometrical shapes and media-responsive optical, electronic, and magnetic properties have attracted significant interest. Nanoscale assembly bridges multiple levels of hierarchy of materials: individual nanoparticles, discrete molecule-like or virus-like nanoscale agglomerates, microscale devices, and macroscale materials. The capacity to self-assemble can greatly facilitate the integration of nanotechnology with other technologies and, in particular, with microscale fabrication. In this Account, we describe developments in the emerging field of dynamic NP assemblies, which are spontaneously form superstructures containing more than two inorganic nanoscale particles that display the ability to change their geometrical, physical, chemical, and other attributes. In many ways, dynamic assemblies can represent a bottleneck in the "bottom-up" fabrication of NP-based devices because they can produce a much greater variety of assemblies, but they also provide a convenient tool for variation of geometries and dimensions of nanoparticle assemblies.Superstructures of NPs (and those held together by similar intrinsic forces)are classified into two groups: Class 1 where media and external fields can alter shape, conformation, and order of stable super structures with a nearly constant number of NPs or Class 2 where the total number of NPs changes, while the organizational motif in the final superstructure remains the same. The future development of successful dynamic assemblies requires understanding the equilibrium in dynamic NP systems. The dynamic nature of Class 1 assemblies is associated with the equilibrium between different conformations of a superstructure and is comparable to the isomerization in classical chemistry. Class 2 assemblies involve the formation or breakage of linkages between the NPs, which is analogous to the classical chemical equilibrium for the formation of a molecule from atoms. Finer classification of NP assemblies in accord with established conventions in the field may include different size dimensionalities: discrete assemblies (artificial molecules) and one-dimensional (spaced chains), two-dimensional (sheets), and three-dimensional (superlattices, twisted structures) assemblies. Notably, these dimensional attributes must be regarded as primarily topological in nature because all of these superstructures can acquire complex three-dimensional shapes.We discuss three primary strategies used to prepare NP superstructures: (1) anisotropy-based assemblies utilizing either intrinsic force field anisotropy around NPs or external anisotropy associated with templates or applied fields, (2) assembly methods utilizing uniform NPs with isotropic interactions, and (3) methods based on mutual recognition of biomolecules, such as DNA and antigen-antibody interactions.We consider optical, electronic, and magnetic properties of dynamic superstructures, focusing primarily on multiparticle effects in NP superstructures as represented by surface plasmon resonance, NP-NP charge transport, and multibody magnetization. Unique properties of NP superstructures are being applied to biosensing, drug delivery, and nanoelectronics. For both Class 1 and Class 2 dynamic assemblies, biosensing is the most dominant and well-developed area of dynamic nanostructures being successfully transitioned into practice. We can foresee the rapid development of dynamic NP assemblies toward applications in harvesting of dissipated energy, photonics, and electronics. The final part of this Account is devoted to the fundamental questions facing dynamic assemblies of NPs in the future. © 2012 American Chemical Society.


Wang Y.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
LWT - Food Science and Technology | Year: 2012

Green banana flour (GBF), as a functional ingredient, was used to partially substitute cassava starch in two types of snacks, viz. fish cracker (FC) and cassava cracker (CC). It is observed that use of GBF increased the nutritional value including enhancement of dietary fiber, essential minerals, polyphenol content, antioxidant capacity and decreased oil content to varying degrees depending on the substitution level in these two crackers. However, its use compromised the textural properties in terms of the linear expansion ratio, crispness, bulk density, porosity and color. Sensory evaluation showed that the products with not more than 40 g substitution level by GBF from 100 g cassava starch in CC and not more than 15 g in FC were acceptable by the panelists. © 2011 Elsevier Ltd.


Daou C.,State Key Laboratory of Food Science and Technology | Zhang H.,Jiangnan University | Zhang H.,Sudan University of Science and Technology
Comprehensive Reviews in Food Science and Food Safety | Year: 2012

This article presents an overview of the recent advances into the health promoting potentials of oat β-glucan. Oat β-glucan (OβG) consists mainly of the linear polysaccharide (1→3), (1→4)-β-D-glucan and is often called β-glucan. This soluble oat fiber is able to attenuate blood postprandial glycemic and insulinemic responses, to lower blood total cholesterol and low-density lipoprotein (LDL) cholesterol, and to improve high-density lipoprotein (HDL) cholesterol and blood lipid profiles as well as to maintain body weight. Thus, OβG intake is beneficial in the prevention, treatment, and control of diabetes and cardiovascular diseases. In addition, OβG can stimulate immune functions by activating monocytes/macrophages and increasing the amounts of immunoglobulin, NK cells, killer T-cells, and so on, which will improve resistance to cancer and infectious and parasitic diseases, as well as increase biological therapies and their prevention. All these health benefits of OβG may be explained by its physicochemical properties (such as viscosity, molecular weight) which can be affected by extraction methods and its behavior in gastrointestinal tract. Articles documenting these health benefits and effects are reviewed. © 2012 Institute of Food Technologists®.


Chen J.,Jiangnan University | Zhang Y.,Wuxi Institute of Technology | Ding R.,Jiangnan University
Mathematical and Computer Modelling | Year: 2010

This paper considers the identification problem for multi-input multi-output nonlinear systems. The difficulty of the parameter identification of such systems is that the information vector in the identification model contains unknown variables. The solution is using the auxiliary model identification idea to overcome the difficulty. An auxiliary model based multi-innovation extended stochastic gradient algorithm is presented by expanding the innovation vector to an innovation matrix. The proposed algorithm uses not only the current innovation but also the past innovations at each recursion and thus the parameter estimation accuracy can be improved. The numerical example shows that the proposed algorithm is effective. © 2010 Elsevier Ltd.


Zhou J.,Jiangnan University | Yin X.,Jiangnan University | Madzak C.,French National Institute for Agricultural Research | Du G.,Jiangnan University | Chen J.,Jiangnan University
Journal of Biotechnology | Year: 2012

α-Ketoglutarate (α-KG) is an important intermediate in the tricarboxylic acid (TCA) cycle and has an important role in the regulation of the balance between carbon and nitrogen metabolism in most microorganisms. In previous research, a thiamine-auxotrophic yeast for α-KG overproduction was screened and named as Yarrowia lipolytica WSH-Z06. To enhance α-KG production and reduce by-product (mainly pyruvate) accumulation, the cofactor metabolism was regulated to redistribute the carbon flux from pyruvate to α-KG. The acetyl-CoA synthetase gene, ACS1, from Saccharomyces cerevisiae and the ATP-citrate lyase gene, ACL, from Mus musculus were expressed to regulate the acetyl-CoA metabolism in Y. lipolytica WSH-Z06. The resultant strains were designated as Y. lipolytica-ACS1 and Y. lipolytica-ACL, respectively. Both of the ACS1 and ACL genes could increase the level of acetyl-CoA and enhance the α-KG production. In a 3-L jar fermenter, the highest yield of α-KG in Y. lipolytica-ACL reached up to 56.5gL-1 with an obvious decrease of pyruvate accumulation from 35.1gL-1 to 20.2gL-1. This study demonstrated that enhancing the acetyl-CoA availability could effectively increase the α-KG production in Y. lipolytica. © 2012 Elsevier B.V.


Yan Q.,Jiangnan University | Zhao M.,Jiangnan University | Miao H.,Jiangnan University | Ruan W.,Jiangnan University | Song R.,Shanghai University
Bioresource Technology | Year: 2010

Coupling bio-production of hydrogen and polyhydroxyalkanoates (PHA) from Taihu blue algae through metabolites circulation was investigated. It was found that the pH adjustment, especially basification was more practical and efficient than other methods for the pretreatment of blue algae before anaerobic digestion. On this occasion, SCOD, biogas accumulation and hydrogen content reached 26 mg/gTS, 500 mL and 37.2%, and which were 4.3, 1.3 and 14.4 times of those of the control group, respectively. Secondly, amounts of both butyric acid and hydrogen could be further increased when blue algae was alkali pretreated at pH 13, as the accumulation of butyric acid, acetic acid and hydrogen reached 1.7, 1.4 and 3.8 times compared to those of the control, respectively. Finally, the coupling bio-production of hydrogen and PHA was conducted through pumping organic residues into PHA fermenter from anaerobic digester. Remarkably, it was found that the larger the pumping rate of carbon and nitrogen sources supply, the higher the yield of DCW and PHA could be expected by Bacillus cereus. © 2010.


Ding J.,Jiangnan University | Shi Y.,University of Victoria | Wang H.,Northwestern Polytechnical University | Ding F.,Jiangnan University
Digital Signal Processing: A Review Journal | Year: 2010

In this paper, we propose a novel identification algorithm for a class of dual-rate sampled-data systems whose input-output data are measured by two different sampling rates. A polynomial transformation technique is employed to derive a mathematical model for such dual-rate systems. The proposed modified stochastic gradient algorithm has faster convergence rate than stochastic gradient algorithms for parameter identification using the dual-rate input-output data. Convergence properties of the algorithm are analyzed. Finally, illustrative and comparison examples are provided to verify the effectiveness and performance improvement of the proposed method. © 2009 Elsevier Inc. All rights reserved.


Ding F.,Jiangnan University
JVC/Journal of Vibration and Control | Year: 2011

The stochastic gradient identification algorithm has slow convergence rates. This paper presents a modified stochastic gradient algorithm by introducing a convergence index in order to improve the convergence rate of the parameter estimation. The parameter estimation accuracy can be enhanced by choosing the convergence index and a numerical example shows that the proposed method is efficient. © The Author(s) 2010.


Ding F.,Jiangnan University | Wang Y.,Jiangnan University | Ding J.,Nanjing University of Posts and Telecommunications
Digital Signal Processing: A Review Journal | Year: 2015

This paper focuses on the parameter estimation problems of output error autoregressive systems and output error autoregressive moving average systems (i.e., the Box-Jenkins systems). Two recursive least squares parameter estimation algorithms are proposed by using the data filtering technique and the auxiliary model identification idea. The key is to use a linear filter to filter the input-output data. The proposed algorithms can identify the parameters of the system models and the noise models interactively and can generate more accurate parameter estimates than the auxiliary model based recursive least squares algorithms. Two examples are given to test the proposed algorithms. © 2014 Elsevier Inc. All rights reserved.


Toure A.,Institute Polytechnic | Xueming X.,Jiangnan University
Comprehensive Reviews in Food Science and Food Safety | Year: 2010

Lignans are compounds found in a variety of plant materials including flaxseed, pumpkin seed, sesame seed, soybean, broccoli, and some berries. The major lignan in flaxseed is called secoisolariciresinol diglucoside (SDG).Once ingested, SDGis converted in the colon into active mammalian lignans, enterodiol, and entero-lactone, which have shown promise in reducing growth of cancerous tumors, especially hormone-sensitive ones such as those of the breast, endometrium, and prostate. Known for their hydrogen-donating antioxidant activity as well as their ability to complex divalent transition metal cations, lignans are propitious to human health. The extraction methods vary fromsimple to complex depending on extraction, separation, fractionation, identification, and detection of the analytes. Flax lignan is also a source of useful biologically active components found in plant foods, such as phytochemicals, and it is considered a functional food. The safety issues in flaxseed are also briefly discussed. © 2010 Institute of Food Technologists®.


Liu Y.,Jiangnan University | Ding F.,Jiangnan University | Shi Y.,University of Victoria
Automatica | Year: 2014

For the lifted input-output representation of general dual-rate sampled-data systems, this paper presents a decomposition based recursive least squares (D-LS) identification algorithm using the hierarchical identification principle. Compared with the recursive least squares (RLS) algorithm, the proposed D-LS algorithm does not require computing the covariance matrices with large sizes and matrix inverses in each recursion step, and thus has a higher computational efficiency than the RLS algorithm. The performance analysis of the D-LS algorithm indicates that the parameter estimates can converge to their true values. A simulation example is given to confirm the convergence results. © 2014 Elsevier Ltd. All rights reserved.


Wang R.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
LWT - Food Science and Technology | Year: 2010

The objective of this work was to study the effect of three different food ingredients viz. NaCl content (1e15 g/100 gwater), sucrose content (5e25 g/100 gwater) and sodium glutamate content (0.5e10 g/100 g water) on microwave freeze drying (MFD) of instant vegetable soup. Response surface methodology (RSM) was employed to optimize their addition levels in instant vegetable soup for MFD. Finally, a comparison of MFD for instant vegetable soup with and without added ingredients was carried out. The results showed that NaCl content and sucrose content had significant influence on drying time and sensory quality, hile sodium glutamate content had insignificant effect. The optimum ingredients of the instant vegetable soup for MFD were: NaCl content of 3.2e5.3 g/100 g water, sucrose content of 2e6.8 g/100 g water and sodium glutamate content of below 4.5 g/100 g water. Comparing with MFD vegetable soup without ingredients, condiments addition could increase drying rate and shorten drying time. © 2010 Elsevier Ltd.


Duan X.,Jiangnan University | Duan X.,Henan University of Science and Technology | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore | Wang S.,Washington State University
Journal of Food Engineering | Year: 2010

Freeze drying (FD) yields the best quality of dried sea cucumber but at the cost of long drying time and also the overall cost. Air drying (AD) gives an unacceptably poor quality product. To achieve faster drying along with a high quality product a microwave freeze drying (MFD) technique was developed to dry sea cucumbers. The relationship between corona discharge and microwave power at various pressures and initial moisture content conditions was studied to avoid the possibility of corona discharge during MFD. According to the drying characteristics of MFD, a control strategy for the MFD process was also developed. MFD reduced the drying time by about half of conventional FD process and provided a similar good product quality. © 2009 Elsevier Ltd. All rights reserved.


Chen J.,Jiangnan University | Zhang Y.,Wuxi Institute of Technology | Ding R.,Jiangnan University
Nonlinear Dynamics | Year: 2013

This paper presents a gradient-based iterative identification algorithm and an auxiliary-model-based multi-innovation generalized extended stochastic gradient algorithm for input nonlinear systems with autoregressive moving average (ARMA) noises, i.e., the input nonlinear Box-Jenkins (IN-BJ) systems. The estimation errors given by the gradient-based iterative algorithm are smaller than the generalized extended stochastic gradient algorithm under same data lengths. A simulation example is provided. © 2013 Springer Science+Business Media Dordrecht.


Chen X.,Jiangnan University | Zhou L.,Jiangnan University | Tian K.,Jiangnan University | Kumar A.,Durban University of Technology | And 3 more authors.
Biotechnology Advances | Year: 2013

In order to decrease carbon emissions and negative environmental impacts of various pollutants, more bulk and/or fine chemicals are produced by bioprocesses, replacing the traditional energy and fossil based intensive route. The Gram-negative rod-shaped bacterium, Escherichia coli has been studied extensively on a fundamental and applied level and has become a predominant host microorganism for industrial applications. Furthermore, metabolic engineering of E. coli for the enhanced biochemical production has been significantly promoted by the integrated use of recent developments in systems biology, synthetic biology and evolutionary engineering. In this review, we focus on recent efforts devoted to the use of genetically engineered E. coli as a sustainable platform for the production of industrially important biochemicals such as biofuels, organic acids, amino acids, sugar alcohols and biopolymers. In addition, representative secondary metabolites produced by E. coli will be systematically discussed and the successful strategies for strain improvements will be highlighted. Moreover, this review presents guidelines for future developments in the bio-based chemical production using E. coli as an industrial platform. © 2013 Elsevier Inc.


Luan X.,Jiangnan University | Shi P.,University of South Wales | Shi P.,Victoria University of Melbourne | Shi P.,University of Adelaide | Liu F.,Jiangnan University
IET Control Theory and Applications | Year: 2013

The problems of finite-time analysis and design for a class of Markov jump systems with Gaussian transition probabilities (TPs) are investigated in this study. Gaussian TP density function is introduced to quantise the stochastic uncertain information of TPs. Mode-dependent and variation-dependent controller is designed to make the resulting closedloop systems finite-time bounded and finite-time stabilisable for all admissible unknown external disturbances and random uncertain TPs. It is shown that the approach proposed in the paper dealing with TPs outperforms available ones in literature to date, which is also confirmed by a numerical example. © The Institution of Engineering and Technology 2013.


Liang R.,Jiangnan University | Liang R.,Rutgers University | Shoemaker C.F.,University of California at Davis | Yang X.,Rutgers University | And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Oil-in-water nanoemulsions stabilized by food-grade biopolymer emulsifiers (modified starches) were fabricated using high-pressure homogenization in an effort to improve the stability and bioaccessibility of β-carotene. Physicochemical and biological properties of β-carotene nanoemulsions were investigated considering the particle size, β-carotene retention, and in vitro digestion. During 30 days of storage at different conditions, the mean diameters of the emulsion systems were increased by 30-85%. The retention of β-carotene in nanoemulsions was significantly higher compared to that of the β-carotene dispersed in bulk oil. After in vitro digestion, the bioaccessibility of β-carotene was increased from 3.1% to 35.6% through nanoencapsulation. The results also indicated that modified starch with high dispersed molecular density led to a higher retention but lower bioaccessibility of β-carotene in nanoemulsions. This could be due to the thick and dense interfacial layer around the oil droplets. This result provides useful information for developing protection and delivery systems for carotenoids. © 2013 American Chemical Society.


Wang Y.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
Drying Technology | Year: 2011

Fresh aquatic products are rich in nutritional compounds and are an important source of good protein for humans. Dehydration processing technologies of aquatic products used in practice are a mix of old and new technologies. With the rapid development of drying technologies and steadily increasing living standards, drying processing techniques as well as types of products produced have changed fundamentally. Two broad types of dried aquatic products are commercially available on a large scale: traditional products and snack foods. In this article, the development of dried aquatic product processing technologies will be expounded along with an overview of the necessary quality attributes and control strategies to produce high-quality dried products of aquatic origin. © 2011 Taylor & Francis Group, LLC.


Zhang F.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
Drying Technology | Year: 2011

The drying characteristics of restructured wild cabbage chips dried using microwave vacuum (MVD), hot air (AD), and microwave freeze drying (MFD) were compared. Some of the key quality parameters of restructured wild cabbage chips such as fracturability and color and sensory characteristics were measured. Results showed that the drying time was reduced with the increase of microwave power (MVD/MFD) and in the case of air drying by the temperature (AD). Drying time was the shortest in the MVD process. Optimal quality of dried chips was obtained with the MFD process at a microwave power level 2.0 W/g. © 2011 Taylor & Francis Group, LLC.


Shi P.,University of Adelaide | Shi P.,Victoria University of Melbourne | Yin Y.,Jiangnan University | Liu F.,Jiangnan University | Zhang J.,North China Electrical Power University
Information Sciences | Year: 2014

In this paper, a robust H∞ controller is designed for saturated Markov jump systems with uncertainties and time varying transition probabilities. The time-varying transition probability uncertainty is described as a polytope set. Stochastic stability is analyzed for the underlying systems by Lyapunov function approach and a sufficient condition is derived to design controllers such that the resulting closed-loop system is stochastically stable and a prescribed H∞ performance is also achieved. Furthermore, the attraction domain of this Markov jump system is estimated and evaluated. A simulation example is given to show the effectiveness of the developed techniques. © 2013 Elsevier Inc. All rights reserved.


Jiang H.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore | Lim R.-X.,Jiangnan University
Food and Bioprocess Technology | Year: 2013

Compared with freeze drying (FD), microwave freeze drying (MFD) using microwave as the heating source can accelerate the drying rate of bananas. This paper presents the results of an experimental study of temperature distribution during the process of MFD banana chips. The results show that the temperature distribution is uniform during the sublimation drying stage, but not at the start of the desorption stage of drying; this phase is prone to generate hot spots, which are brought about by the localized melting of ice crystals not removed in the sublimation stage and the absorption of excess microwave by sorbed water. Comparison of scanning electron microscope micrographs of FD and MFD samples shows that the major dissimilarity between the two samples is in their pore sizes. © 2012 Springer Science+Business Media, LLC.


Wang Y.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore | Mothibe K.J.,Jiangnan University
Food and Bioprocess Technology | Year: 2013

Drying uniformity, microstructure, apparent density, rehydration, and texture properties were measured to evaluate the quality of stem lettuce slices dried in a pulse-spouted bed microwave freeze dryer. Drying was carried out in a 5-cm (o.d.) vacuum chamber at 80 ± 5 Pa, mean microwave power level of 3.2 W g-1 and pulse-spouting time interval of 10 min. Results show that microwave freeze-dried products in the pulse-spouted mode dried more uniformly as compared to those dried in steady spouted bed mode. Pulse-spouted bed mode also resulted in dried stem lettuce slices with lower discoloration, more uniform and compact microstructure, higher rehydration capacity (RC) as well as greater hardness after rehydration over shorter drying time relative to those obtained in a steady spouting condition. © 2012 Springer Science+Business Media New York.


Yin Y.,Jiangnan University | Yin Y.,Curtin University Australia | Shi P.,University of Adelaide | Shi P.,Victoria University of Melbourne | And 2 more authors.
Information Sciences | Year: 2014

This paper studies the problem of robust HO filtering for a class of uncertain discrete-time nonhomogeneous Markov jump systems. The time-varying jump transition probability matrix is described by a polytope. By Lyapunov function approach, mode-dependent and variation-dependent HO filter is designed such that the resulting error dynamic system is stochastically stable and has a prescribed HO performance index. A numerical example is given to illustrate the effectiveness of the developed techniques. © 2013 Elsevier Inc. All rights reserved.


Liu Y.,Jiangnan University | Ding F.,Jiangnan University | Shi Y.,University of Victoria
Circuits, Systems, and Signal Processing | Year: 2012

This paper presents a novel hierarchical least squares algorithm for a class of non-uniformly sampled systems. Based on the hierarchical identification principle, the identification model with a high dimensional parameter vector is decomposed into a group of submodels with lower dimensional parameter vectors. By using the least squares method to identify the submodels and taking a coordinated measure to address the associated items between the submodels, all the system parameters can be estimated. The proposed algorithm can save the computation cost. The performance analysis indicates that parameter estimates converge to their true values. The simulation tests confirm the convergence results. © Springer Science+Business Media, LLC 2012.


Hao C.,Jiangnan University | Xua L.,Jiangnan University | Xing C.,Jiangnan University | Kuang H.,Jiangnan University | Xu C.,Jiangnan University
Biosensors and Bioelectronics | Year: 2012

In this study, we report a new fluorogenic sensor based on fluorescence resonance energy transfer (FRET) for detection of heavy metal ions in aqueous solution. The method showed the advantage of being simple, highly sensitive and selective, and rapid. The donor (CdTe QDs) and acceptor (TAMRA or Cy5) are brought into close proximity to one another due to Hg 2+ and Ag + form strong and stable T-Hg 2+-T complexes and C-Ag +-C complexes, which quenches the fluorescent intensity of CdTe QDs and enables the energy transfer from donor to acceptor. This sensor showed high sensitivity and selectivity when only one kind of ion (Ag + or Hg 2+) exists. Furthermore, the assay can also simultaneously detect Ag + and Hg 2+ in water media with the limit of detection (LOD) of 2.5 and 1.8nM, separately, which satisfactorily meets the sensitivity demands of Environmental Protection Agency (EPA) and World Health Organization (WHO). This assay also exhibits excellent selectivity toward Ag + and Hg 2+. Therefore, this method is of great practical and theoretical importance for detecting heavy metal ions in aqueous solution. © 2012 Elsevier B.V.


Chen J.,Jiangnan University | Ding R.,Jiangnan University
Circuits, Systems, and Signal Processing | Year: 2013

This paper presents an identification algorithm for Box-Jenkins systems by combining the auxiliary model identification idea and the gradient search principle. The proposed algorithm can estimate all unknown parameters of the Box-Jenkins systems. Furthermore, to improve the convergence rate of the stochastic gradient algorithm, a modified stochastic gradient algorithm is given. The simulation results indicate that the proposed algorithm can work well. © 2013 Springer Science+Business Media New York.


Wang R.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
Journal of Food Engineering | Year: 2010

Potato slices immersed in 0.5% CaCl2 solution for 10 min were examined using light microscopy for their microstructure in frozen state before drying, during the sublimation drying stage, in the desorption drying stage and in the final dried form. Additionally, the final dried product was also tested for vitamin C content, color, starch content, texture and sugar content. Experiments were carried out using conventional vacuum freeze dryer as well as a microwave freeze dryer. Results for both unblanched and blanched potato tissues, crystal growth during sublimation drying stage was observed to cause structural damage to the cell walls. Blanched tissue suffered more damage during the freezing process. Interestingly, microwave freeze drying yielded product similar in quality to that obtained in vacuum freeze drying with conductive heating. © 2010 Elsevier Ltd. All rights reserved.


Jiang H.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,National University of Singapore
Journal of Food Engineering | Year: 2010

Microwave freeze drying (MFD) normally contains three stages, i.e., pre-freeze stage, primary drying stage and secondary drying stage, just as in conventional freeze drying. This research examines the variations of structure, starch content, reducing sugar content, color change as well as expansion ratio during these stages of microwave freeze drying banana chips. Results show that biggest changes are found at the primary drying stage in the banana slices' starch content, reduced sugar content, their structure and their changed colors while the biggest change of expansion ratio occurs at the secondary drying stage. Similar results can also be obtained for FD samples. Thus it is concluded that the primary stage can do most damage to the banana chips. © 2010 Elsevier Ltd. All rights reserved.


Wearable, textiles-based electronics present new possibilities for flexible circuits, healthcare and environment monitoring, energy conversion, and many others. Now, researchers at the Cambridge Graphene Centre (CGC) at the University of Cambridge, working in collaboration with scientists at Jiangnan University, China, have devised a method for depositing graphene-based inks onto cotton to produce a conductive textile. The work, published in the journal Carbon, demonstrates a wearable motion sensor based on the conductive cotton. Cotton fabric is among the most widespread for use in clothing and textiles, as it is breathable and comfortable to wear, as well as being durable to washing. These properties also make it an excellent choice for textile electronics. A new process, developed by Dr Felice Torrisi at the CGC, and his collaborators, is a low-cost, sustainable and environmentally-friendly method for making conductive cotton textiles by impregnating them with a graphene-based conductive ink. Based on Dr Torrisi's work on the formulation of printable graphene inks for flexible electronics, the team created inks of chemically modified graphene flakes that are more adhesive to cotton fibres than unmodified graphene. Heat treatment after depositing the ink on the fabric improves the conductivity of the modified graphene. The adhesion of the modified graphene to the cotton fibre is similar to the way cotton holds coloured dyes, and allows the fabric to remain conductive after several washes. Although numerous researchers around the world have developed wearable sensors, most of the current wearable technologies rely on rigid electronic components mounted on flexible materials such as plastic films or textiles. These offer limited compatibility with the skin in many circumstances, are damaged when washed and are uncomfortable to wear because they are not breathable. "Other conductive inks are made from precious metals such as silver, which makes them very expensive to produce and not sustainable, whereas graphene is both cheap, environmentally-friendly, and chemically compatible with cotton," explains Dr Torrisi. Co-author Professor Chaoxia Wang of Jiangnan University adds: "This method will allow us to put electronic systems directly into clothes. It's an incredible enabling technology for smart textiles." The work done by Dr Torrisi and Prof Wang, together with students Tian Carey and Jiesheng Ren, opens a number of commercial opportunities for graphene-based inks, ranging from personal health technology, high-performance sportswear, military garments, wearable technology/computing and fashion. "Turning cotton fibres into functional electronic components can open to an entirely new set of applications from healthcare and wellbeing to the Internet of Things," says Dr Torrisi "Thanks to nanotechnology, in the future our clothes could incorporate these textile-based electronics and become interactive." Graphene is carbon in the form of single-atom-thick membranes, and is highly conductive. The group's work is based on the dispersion of tiny graphene sheets, each less than one nanometre thick, in a water-based dispersion. The individual graphene sheets in suspension are chemically modified to adhere well to the cotton fibres during printing and deposition on the fabric, leading to a thin and uniform conducting network of many graphene sheets. This network of nanometre flakes is the secret to the high sensitivity to strain induced by motion. A simple graphene-coated smart cotton textile used as a wearable strain sensor has been shown to reliably detect up to 500 motion cycles, even after more than 10 washing cycles in normal washing machine. The use of graphene and other related 2D materials (GRMs) inks to create electronic components and devices integrated into fabrics and innovative textiles is at the centre of new technical advances in the smart textiles industry. Dr Torrisi and colleagues at the CGC are also involved in the Graphene Flagship, an EC-funded, pan-European project dedicated to bringing graphene and GRM technologies to commercial applications. Graphene and GRMs are changing the science and technology landscape with attractive physical properties for electronics, photonics, sensing, catalysis and energy storage. Graphene's atomic thickness and excellent electrical and mechanical properties give excellent advantages, allowing deposition of extremely thin, flexible and conductive films on surfaces and – with this new method – also on textiles. This combined with the environmental compatibility of graphene and its strong adhesion to cotton make the graphene-cotton strain sensor ideal for wearable applications. The research was supported by grants from the European Research Council's Synergy Grant, the International Research Fellowship of the National Natural Science Foundation of China and the Ministry of Science and Technology of China. The technology is being commercialised by Cambridge Enterprise, the University's commercialisation arm. Explore further: New study shows nickel graphene can be tuned for optimal fracture strength More information: Jiesheng Ren et al. Environmentally-friendly conductive cotton fabric as flexible strain sensor based on hot press reduced graphene oxide, Carbon (2017). DOI: 10.1016/j.carbon.2016.10.045


News Article | November 28, 2016
Site: www.cemag.us

A new method for producing conductive cotton fabrics using graphene-based inks opens up new possibilities for flexible and wearable electronics, without the use of expensive and toxic processing steps. Wearable, textiles-based electronics present new possibilities for flexible circuits, healthcare and environment monitoring, energy conversion, and many others. Now, researchers at the Cambridge Graphene Centre (CGC) at the University of Cambridge, working in collaboration with scientists at Jiangnan University, China, have devised a method for depositing graphene-based inks onto cotton to produce a conductive textile. The work, published in the journal Carbon, demonstrates a wearable motion sensor based on the conductive cotton. Cotton fabric is among the most widespread for use in clothing and textiles, as it is breathable and comfortable to wear, as well as being durable to washing. These properties also make it an excellent choice for textile electronics. A new process, developed by Dr. Felice Torrisi at the CGC, and his collaborators, is a low-cost, sustainable and environmentally-friendly method for making conductive cotton textiles by impregnating them with a graphene-based conductive ink. Based on Torrisi’s work on the formulation of printable graphene inks for flexible electronics, the team created inks of chemically modified graphene flakes that are more adhesive to cotton fibers than unmodified graphene. Heat treatment after depositing the ink on the fabric improves the conductivity of the modified graphene.  The adhesion of the modified graphene to the cotton fiber is similar to the way cotton holds colored dyes, and allows the fabric to remain conductive after several washes. Although numerous researchers around the world have developed wearable sensors, most of the current wearable technologies rely on rigid electronic components mounted on flexible materials such as plastic films or textiles. These offer limited compatibility with the skin in many circumstances, are damaged when washed and are uncomfortable to wear because they are not breathable. “Other conductive inks are made from precious metals such as silver, which makes them very expensive to produce and not sustainable, whereas graphene is both cheap, environmentally-friendly, and chemically compatible with cotton,” explains Torrisi. Co-author Professor Chaoxia Wang of Jiangnan University adds, “This method will allow us to put electronic systems directly into clothes. It’s an incredible enabling technology for smart textiles.” The work done by Torrisi and Wang, together with students Tian Carey and Jiesheng Ren, opens a number of commercial opportunities for graphene-based inks, ranging from personal health technology, high-performance sportswear, military garments, wearable technology/computing, and fashion. “Turning cotton fibers into functional electronic components can open to an entirely new set of applications from healthcare and wellbeing to the Internet of Things,” says Torrisi “Thanks to nanotechnology, in the future our clothes could incorporate these textile-based electronics and become interactive.” Graphene is carbon in the form of single-atom-thick membranes, and is highly conductive. The group’s work is based on the dispersion of tiny graphene sheets, each less than one nanometer thick, in a water-based dispersion. The individual graphene sheets in suspension are chemically modified to adhere well to the cotton fibers during printing and deposition on the fabric, leading to a thin and uniform conducting network of many graphene sheets. This network of nanometer flakes is the secret to the high sensitivity to strain induced by motion. A simple graphene-coated smart cotton textile used as a wearable strain sensor has been shown to reliably detect up to 500 motion cycles, even after more than 10 washing cycles in normal washing machine. The use of graphene and other related 2D materials (GRMs) inks to create electronic components and devices integrated into fabrics and innovative textiles is at the center of new technical advances in the smart textiles industry. Torrisi and colleagues at the CGC are also involved in the Graphene Flagship, an EC-funded, pan-European project dedicated to bringing graphene and GRM technologies to commercial applications. Graphene and GRMs are changing the science and technology landscape with attractive physical properties for electronics, photonics, sensing, catalysis and energy storage. Graphene’s atomic thickness and excellent electrical and mechanical properties give excellent advantages, allowing deposition of extremely thin, flexible and conductive films on surfaces and — with this new method — also on textiles. This combined with the environmental compatibility of graphene and its strong adhesion to cotton make the graphene-cotton strain sensor ideal for wearable applications. The research was supported by grants from the European Research Council’s Synergy Grant, the International Research Fellowship of the National Natural Science Foundation of China and the Ministry of Science and Technology of China. The technology is being commercialized by Cambridge Enterprise, the University’s commercialization arm.


Rao S.,Yangzhou University | Sun J.,Jiangnan University | Liu Y.,Jiangnan University | Zeng H.,Angel Yeast Co. | And 2 more authors.
Food Chemistry | Year: 2012

Lysozyme from hen egg white is a well-known antimicrobial protein with high ratio of hydrophobic and positively charged amino acid residues. In order to explore functional bioactivities of enzymatic hydrolysates of lysozyme, the protein was subjected to a simulated gastrointestinal digestion and the resulting hydrolysate (LPH2) showed a strong competitive angiotensin I-converting enzyme (ACE) inhibitory activity (IC50 = 12.6 μg/ml) and a remarkable antioxidant activity. The LPH2 was fractionated using a 3 kDa cut-off membrane and the obtained permeate LPH2-3 kDa was analysed by MALDI-TOF-TOF MS. Using this technology, 38 different peptides were identified and some of these peptides were well fit with structure requirements of ACE inhibitory peptides and/or antioxidant peptides. The findings from this study suggest that the protein containing high proportion of hydrophobic and positively charged residues have the potential to generate multifunctional peptides, and these peptides would be beneficial ingredient to be used in functional foods. © 2012 Elsevier Ltd. All rights reserved.


Xu J.,Jiangnan University | Zhang J.,OriGene Biotechnology Co. | Guo Y.,Jiangnan University | Zai Y.,Jiangnan University | Zhang W.,Jiangnan University
Journal of Industrial Microbiology and Biotechnology | Year: 2013

Fructose-1,6-bisphosphatase (FBPase) and fructokinase (ScrK) have important roles in regenerating glucose-6-phosphate in the pentose phosphate pathway (PPP), and thus increasing L-lysine production. This article focuses on the development of L-lysine high-producing strains by heterologous expression of FBPase gene fbp and ScrK gene scrK in C. glutamicum lysCfbr with molasses as the sole carbon source. Heterologous expression of fbp and scrK lead to a decrease of residual sugar in fermentation broth, and heterologous expression of scrK prevents the fructose efflux. Heterologous expression of fbp and scrK not only increases significantly the activity of corresponding enzymes but also improves cell growth during growth on molasses. FBPase activities are increased tenfold by heterologous expression of fbp, whereas the FBPase activity is only increase fourfold during coexpression of scrK and fbp. Compared with glucose, the DCW of heterologous expression strains are higher on molasses except co-expression of fbp and scrK strain. In addition, heterologous expression of fbp and scrK can strongly increase the L-lysine production with molasses as the sole carbon source. The highest increase (88.4 %) was observed for C. glutamicum lysCfbr pDXW-8-fbp-scrK, but the increase was also significant for C. glutamicum lysCfbr pDXW-8-fbp (47.2 %) and C. glutamicum lysCfbr pDXW- 8-scrK (36.8 %). By-products, such as glycerol and dihydroxyacetone, are decreased by heterologous expression of fbp and scrK, whereas trehalose is only slightly increased. The strategy for enhancing L-lysine production by regeneration of glucose-6-phosphate in PPP may provide a reference to enhance the production of other amino acids during growth on molasses or starch.


Ding F.,Jiangnan University | Liu G.,Ryerson University | Liu X.P.,Carleton University
IEEE Transactions on Automatic Control | Year: 2010

This technical note addresses identification problems of non-uniformly sampled systems. For the input-output representation of non-uniform discrete-time systems, a partially coupled stochastic gradient (C-SG) algorithm is proposed to estimate the model parameters with high computational efficiency compared with the standard stochastic gradient (SG) algorithm. The analysis indicates that the partially C-SG algorithm can give more accurate parameter estimates than the SG algorithm. The parameter estimates obtained using the partially C-SG algorithm converge to their true values as the data length approaches infinity. © 2010 IEEE.


Ding F.,Jiangnan University | Liu P.X.,Carleton University | Liu G.,Ryerson University
Digital Signal Processing: A Review Journal | Year: 2010

Gradient based and least-squares based iterative identification algorithms are developed for output error (OE) and output error moving average (OEMA) systems. Compared with recursive approaches, the proposed iterative algorithms use all the measured input-output data at each iterative computation (at each iteration), and thus can produce highly accurate parameter estimation. The basic idea of the iterative methods is to adopt the interactive estimation theory: the parameter estimates relying on unknown variables are computed by using the estimates of these unknown variables which are obtained from the preceding parameter estimates. The simulation results confirm theoretical findings. © 2009 Elsevier Inc. All rights reserved.


Ni X.,Jiangnan University | Xing X.,Yancheng Institute of Technology | Cao Y.,Jiangnan University | Cao G.,Jiangnan University
Journal of Chromatography A | Year: 2014

A novel polymeric micelle, formed by random copolymer poly (stearyl methacrylate- co-methacrylic acid) (P(SMA- co-MAA)) has been used as pseudostationary phase (PSP) in electrokinetic chromatography (EKC) for simultaneous and rapid determination of 11 kinds of water- and fat-soluble vitamins in this work. The running buffer consisting of 1% (w/v) P(SMA- co-MAA), 10% (v/v) 1-butanol, 20% (v/v) acetonitrile, and 30. mM Palitzsch buffer solution (pH 9.2) was applied to improve the selectivity and efficiency, as well as to shorten analysis time. 1-Butanol and acetonitrile as the organic solvent modifiers played the most important roles for rapid separation of these vitamins. The effects of organic solvents on microstructure of the polymeric micelle were investigated. The organic solvents swell the polymeric micelle by three folds, lower down the surface charge density and enhance the microenviromental polarity of the polymeric micelle. The 11 kinds of water- and fat-soluble vitamins could be baseline separated within 13. min. The method was applied to determine water- and fat-soluble vitamins in commercial vitamin sample; the recoveries were between 93% and 111% with the relative standard derivations (RSDs) less than 5%. The determination results matched the label claim. © 2014 Elsevier B.V.


Ding F.,Jiangnan University | Liu P.X.,Carleton University | Liu G.,Ryerson University
IEEE Transactions on Systems, Man, and Cybernetics, Part B: Cybernetics | Year: 2010

A multiinnovation least-squares (MILS) identification algorithm is presented for linear regression models with unknown parameter vectors by expanding the innovation length in the traditional recursive least-squares (RLS) algorithm from the viewpoint of innovation modification. Because the proposed MILS algorithm uses ρ innovations (not only the current innovation but also past innovations) at each iteration (with the integer ρ>1 being an innovation length), the accuracy of parameter estimation is improved, compared with that of the RLS algorithm. Performance analysis and simulation results show that the proposed MILS algorithm is consistently convergent. Moreover, a new interval-varying MILS algorithm is proposed, for which the key is to dynamically change the interval in order to deal with cases where some measurement data are missing. Furthermore, an auxiliary-model-based MILS algorithm is derived for pseudolinear models corresponding to output error moving average systems with colored noises. Finally, the proposed algorithms are applied to model an experimental water level control system. © 2006 IEEE.


Huang L.-l.,Jiangnan University | Huang L.-l.,Shenzhen Polytechnic | Zhang M.,Jiangnan University
Drying Technology | Year: 2012

Diverse types of vegetable snacks made using different drying methods are becoming increasingly commercially important for the food processing industry worldwide because they are also recognized as healthy for human consumption. Here we consider four aspects of drying snacks: unit operations currently used in the industry, novel or emerging methods with nontraditional means, combined (or hybrid) drying methods, and the quality changes during storage. Each drying method has its own advantages and limitations. Hybrid drying techniques are being developed to maximize the benefits of different drying techniques to produce better quality vegetable snacks that are attractive to the consumer. The merits and limitations of more than 10 drying techniques used for making dried vegetable snacks are discussed. Moreover, several new vegetables snacks dried using new drying methods are presented and discussed. A comprehensive review of the recent progress in production of dried vegetable snacks is presented and recommendations are made for future research. © 2012 Taylor and Francis Group, LLC.


Ding J.,Jiangnan University | Ding F.,Jiangnan University | Liu X.P.,Carleton University | Liu G.,Ryerson University
IEEE Transactions on Automatic Control | Year: 2011

This technical note studies identification problems for dual-rate sampled-data linear systems with noises. A hierarchical least squares (HLS) identification algorithm is presented to estimate the parameters of the dual-rate ARMAX models. The basic idea is to decompose the identification model of a dual-rate system into several sub-identification models with smaller dimensions and fewer parameters. The proposed algorithm is more computationally efficient than the recursive least squares (RLS) algorithm since the RLS algorithm requires computing the covariance matrix of large sizes, while the HLS algorithm deals with the covariance matrix of small sizes. Compared with our previous work, a detailed study of the HLS algorithm is conducted in this technical note. The performance analysis and simulation results confirm that the estimation accuracy of the proposed algorithm are close to that of the RLS algorithm, but the proposed algorithm retains much less computational burden. © 2011 IEEE.


Xu Y.X.,Jiangnan University | Zhang M.,Jiangnan University | Fang Z.X.,Curtin University Australia | Sun J.C.,Food Research Institute of Haitong Food Group Corporation Ltd | Wang Y.Q.,Jiangnan University
Food Chemistry | Year: 2014

To improve the flavour quality of bayberry juice, effects of different raw materials, heat treatment and storage time on the flavour variations were investigated. Changes of total sugar and titratable acids were also monitored. Identification and quantitation of volatile flavour compounds were performed by headspace solid-phase microextraction couped with gas chromatography-mass spectrometry (HS-SPME-GC-MS). The contents of esters, alcohols, total sugar and titratable acidity of the rancid flavour raw juice were 6.03%, 8.24%, 5.56 g/L, 0.18 g/L less than those of pleasing flavour raw juice (PF), while the content of aldehydes was 4.19% higher than that of PF. After 9 months storage, the bayberry juice produced fermentation-like flavours with alcohols increases (11.45%) while esters (14.91%) and total sugar (3.27 g/L) decreases. The results suggested that proper juice processing and storage techniques are critical to the flavour quality of bayberry juice. © 2013 Elsevier Ltd. All rights reserved.


Ding F.,Jiangnan University | Liu X.P.,Carleton University | Liu G.,Ryerson University
Digital Signal Processing: A Review Journal | Year: 2011

This paper considers the identification problems of the Hammerstein nonlinear systems. A projection and a stochastic gradient (SG) identification algorithms are presented for the Hammerstein nonlinear systems by using the gradient search method. Since the projection algorithm is sensitive to noise and the SG algorithm has a slow convergence rate, a Newton recursive and a Newton iterative identification algorithms are derived by using the Newton method (Newton-Raphson method), in order to reduce the sensitivity of the projection algorithm to noise, and to improve convergence rates of the SG algorithm. Furthermore, the performances of these approaches are analyzed and compared using a numerical example, including the parameter estimation errors, the stationarity and convergence rates of parameter estimates and the computational efficiency. © 2010 Elsevier Inc. All rights reserved.


Ding F.,Jiangnan University | Liu G.,Ryerson University | Liu X.P.,Carleton University
Automatica | Year: 2011

In this paper, the problems of parameter estimation are addressed for systems with scarce measurements. A gradient-based algorithm is derived to estimate the parameters of the inputoutput representation with scarce measurements, and the convergence properties of the parameter estimation and unavailable output estimation are established using the Kronecker lemma and the deterministic version of the martingale convergence theorem. Finally, an example is provided to demonstrate the effectiveness of the proposed algorithm. © 2011 Elsevier Ltd. All rights reserved.


Ziauddin M.F.,Georgia Regents University | Hua D.,Jiangnan University | Tang S.-C.,Georgia Regents University
Cancer and Metastasis Reviews | Year: 2014

Endocrine therapy of breast cancer is perhaps the oldest form of effective and well-tolerated targeted cancer systemic treatment, in both the adjuvant and metastatic disease settings. The most commonly used endocrine therapy agents are selective estrogen receptor modulators, aromatase inhibitors, and selective estrogen receptor downregulators. De novo or acquired resistance to these agents is a significant clinical problem. Preclinical and clinical investigations to understand this resistance have yielded significant advances in understanding cell signaling and the possible mechanisms of resistance. These mechanisms of resistance are as diverse as the biology of breast cancer and can arise from alterations in any of the cell signaling pathway components. A growing understanding of these mechanisms has provided rationale for development of strategies to overcome the resistance. Many of these mechanisms of resistance involve adaptive upregulation of alternate signaling pathways, such as growth factor signaling, and cross talk between estrogen receptor and growth factor signaling. Clinical trials are focusing on cotargeting these alternate pathways along with estrogen receptor signaling. It is becoming evident that, as with all cancer therapy, strategies to overcome resistance need to be individualized, and it is important to identify biomarkers to guide the use of these strategies. This manuscript systemically reviews the recent preclinical and clinical trials on the novel and pathway-driven agents that have shown significant promise in enhancing the efficacy and overcoming the resistance in the hormonal treatment of breast cancer. Future directions including biomarker selection and the role of next generation sequencing will be discussed. © 2014 Springer Science+Business Media.


Wang X.,Jiangnan University | Quinn P.J.,King's College London | Yan A.,University of Hong Kong
Biological Reviews | Year: 2015

3-deoxy-d-manno-octulosonic acid-lipid A (Kdo2-lipid A) is the essential component of lipopolysaccharide in most Gram-negative bacteria and the minimal structural component to sustain bacterial viability. It serves as the active component of lipopolysaccharide to stimulate potent host immune responses through the complex of Toll-like-receptor 4 (TLR4) and myeloid differentiation protein 2. The entire biosynthetic pathway of Escherichia coli Kdo2-lipid A has been elucidated and the nine enzymes of the pathway are shared by most Gram-negative bacteria, indicating conserved Kdo2-lipid A structure across different species. Yet many bacteria can modify the structure of their Kdo2-lipid A which serves as a strategy to modulate bacterial virulence and adapt to different growth environments as well as to avoid recognition by the mammalian innate immune systems. Key enzymes and receptors involved in Kdo2-lipid A biosynthesis, structural modification and its interaction with the TLR4 pathway represent a clear opportunity for immunopharmacological exploitation. These include the development of novel antibiotics targeting key biosynthetic enzymes and utilization of structurally modified Kdo2-lipid A or correspondingly engineered live bacteria as vaccines and adjuvants. Kdo2-lipid A/TLR4 antagonists can also be applied in anti-inflammatory interventions. This review summarizes recent knowledge on both the fundamental processes of Kdo2-lipid A biosynthesis, structural modification and immune stimulation, and applied research on pharmacological exploitations of these processes for therapeutic development. © 2014 The Authors. Biological Reviews published by John Wiley & Sons Ltd on behalf of Cambridge Philosophical Society.


Wang X.,Jiangnan University | Quinn P.J.,King's College London
Progress in Lipid Research | Year: 2010

Lipopolysaccharide that constitutes the outer leaflet of the outer membrane of most Gram-negative bacteria is referred to as an endotoxin. It is comprised of a hydrophilic polysaccharide and a hydrophobic component referred to as lipid A. Lipid A is responsible for the major bioactivity of endotoxin, and is recognized by immune cells as a pathogen-associated molecule. Most enzymes and genes coding for proteins responsible for the biosynthesis and export of lipopolysaccharide in Escherichia coli have been identified, and they are shared by most Gram-negative bacteria based on genetic information. The detailed structure of lipopolysaccharide differs from one bacterium to another, consistent with the recent discovery of additional enzymes and gene products that can modify the basic structure of lipopolysaccharide in some bacteria, especially pathogens. These modifications are not required for survival, but are tightly regulated in the cell and closely related to the virulence of bacteria. In this review we discuss recent studies of the biosynthesis and export of lipopolysaccharide, and the relationship between the structure of lipopolysaccharide and the virulence of bacteria. © 2009 Elsevier Ltd. All rights reserved.


Xu L.,Applied Technology Internet | Xu L.,Jiangnan University
Signal Processing | Year: 2016

The sine signal is used widely in the signal processing, communication, system analysis and system identification. This paper proposes a damping parameter estimation algorithm for dynamical systems based on the sine frequency response. The measured data are collected by taking the sine signals as the input. Analyzing the system's output sine response function, we can construct a nonlinear objective function. By using the nonlinear optimization techniques, we propose an iterative algorithm to estimate the system parameters. In order to overcome the singular or ill-conditioned matrix during the iterative process, we introduce a damping factor in the proposed iterative algorithm. At the same time the gradient iterative parameter estimation algorithm and the Gauss-Newton iterative parameter estimation algorithm are derived for comparing the performance of the presented methods. Moreover, the simulation results given by an example indicate that the proposed method works well. © 2015 Elsevier B.V.


Xu S.,Jiangnan University | Zhao J.,Nanjing Southeast University
Diangong Jishu Xuebao/Transactions of China Electrotechnical Society | Year: 2011

Based on the irregular sampling method, the harmonic characteristics of the CPS-SPWM output voltage of cascaded H-bridge multilevel converters are analyzed in this paper. Simulation and experimental results show that, on condition that the carrier wave frequency defined as ωc is much larger than the sinusoidal modulation wave frequency, the fundamental component of the CPS-SPWM output voltage will lag the modulation wave a quarter of carrier wave period. Additionally, the odd harmonic components mainly distribute near the even times the number of Nωc, while the even harmonic components are eliminated.


Zhang M.,Leibniz Institute for Catalysis at the University of Rostock | Zhang M.,Jiangnan University | Neumann H.,Leibniz Institute for Catalysis at the University of Rostock | Beller M.,Leibniz Institute for Catalysis at the University of Rostock
Angewandte Chemie - International Edition | Year: 2013

It's a snap: A novel catalytic three-component coupling reaction using simple and easily available substrates leads to a wide range of substituted pyrroles with high regioselectively (see scheme; Xantphos=9,9-dimethyl-4,5- bis(diphenylphosphino)xanthene). Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Roknul A.S.M.,Jiangnan University | Zhang M.,Jiangnan University | Mujumdar A.S.,Hong Kong University of Science and Technology | Wang Y.,Jiangnan University
Drying Technology | Year: 2014

This article presents experimental results and analysis of four drying methods, viz. hot air drying (AD), hot air-assisted radio frequency drying (ARFD), infrared drying (IRD), and microwave-assisted hot air drying (MAD), on color, microstructure, density, rehydration capacity, and texture after rehydration of stem lettuce slices (Lactuca sativa L.). The drying time required for these drying protocols was also compared. These four drying tests were conducted at fixed air temperature (60°C) and velocity (1 m/s), as well as identical sample load (300 g), bed depth (20 mm), and the power level for ARFD, IRD, and MAD, which was fixed at 4 W/g. The results showed that the drying time required for stem lettuce slices using ARFD was the shortest (120 min), followed by MAD (140 min) and IRD (180 min); AD required the longest time (360 min). Notably, ARFD yielded uniform drying and the quality of the dried samples using ARFD was also the best among these four drying methods. © 2014 Copyright Taylor & Francis Group, LLC.


This paper presents a system of projection neural networks (SPNNs) for solving the system of generalized variational inequalities (SGVIs). The SPNNs includes the projection neural network as its special case. Based on the Lyapunov method, the global exponential stability of the SPNNs is derived and proved under some mild conditions. In addition, simulation results on numerical examples show the effectiveness and performance of the proposed SPNNs. © 2010.


Zhang M.,Leibniz Institute for Catalysis at the University of Rostock | Zhang M.,Jiangnan University | Imm S.,Leibniz Institute for Catalysis at the University of Rostock | Bahn S.,Leibniz Institute for Catalysis at the University of Rostock | And 3 more authors.
Angewandte Chemie - International Edition | Year: 2012

Amid(e) them all: Primary carboxamides and ureas react with aromatic and aliphatic amines in the presence of a copper catalyst to give a wide range of functionalized amides (see scheme). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Zhang C.,Jiangnan University | Zhang C.,Nanjing Southeast University | Zhang Z.,Nanjing Southeast University | Shi J.,Nanjing Institute of Technology
Journal of Constructional Steel Research | Year: 2012

Low yield strength steel 100 is widely applied to design shear panel damper for its high ductility. The practical shear strain of low yield strength steel shear panel damper at present is around 12% while the elongation of the LYS100 tends to go around 60%, which indicates that the ductility of LYS100 has not been fully utilized. To develop function separate damper with LYS100, links of the frame fixture, rib shape and panel shape that affect the deformation capacity of the damper were investigated experimentally. The test results show that the deformation capacity can be improved greatly by alleviating the stress concentration locating at the panel corners. The largest shear strain of 70% is achieved by the optimization on the parameters. The large deformation capacity provides a compact, low-cost alternative to structural designers. © 2012 Elsevier Ltd. All rights reserved.


Zhang M.,Leibniz Institute for Catalysis at the University of Rostock | Zhang M.,Jiangnan University | Wu X.-F.,Leibniz Institute for Catalysis at the University of Rostock
Tetrahedron Letters | Year: 2013

The first zinc-catalyzed oxidative amidation of arylaldehydes has been developed. Various amides were prepared in good yields under solvent-free and mild reaction conditions. © 2012 Elsevier Ltd. All rights reserved.


Xu J.,Nanjing Southeast University | Zhang H.,Jiangnan University
Advanced Materials Research | Year: 2013

Appling the design principles and methods of Ergonomics into the modern office furniture design, analysis of the design elements of the modern office furniture in Ergonomics, for the concept of ensuring that human health, safe, comfortable and efficient, provided scientific and rational design method of Ergonomics for the modern office furniture design. © (2013) Trans Tech Publications, Switzerland.


Ji X.-S.,Nanjing Southeast University | Ji X.-S.,Jiangnan University
Dianli Xitong Baohu yu Kongzhi/Power System Protection and Control | Year: 2010

Partial least square (PLS) can decrease the correlation among the power load information. The least square support vector machine (LS-SVM) can get the global optional forecasting result of the power load and decrease the computation burden. Principles of the PLS, LS-SVM and PLS-LS-SVM are introduced. PLS-LS-SVM is used to establish short-term daily power load prediction forecast model and then the model is applied to the daily power load forecast in a certain area in 2008. Mean relative forecasting error and maximal relative forecasting error are 0.685 percent and 8.8599 percent respectively. Compared with the AR(1) model, excellent forecasting accuracy of the PLS-LS-SVM model can supply the short-term power load forecasting an effective way.


Hu A.,Nanjing Southeast University | Hu A.,Jiangnan University | Cao J.,Nanjing Southeast University | Cao J.,King Abdulaziz University | And 2 more authors.
Physica A: Statistical Mechanics and its Applications | Year: 2014

This paper is concerned with the issue of mean square cluster synchronization in directed networks, which consist of non-identical nodes infected by communication noises. The pinning control method is employed in designing controllers for guaranteeing cluster synchronization, meanwhile, all the controllers are supposed to occur with different probabilities by introducing the Bernoulli stochastic variables. Based on the Lyapunov stability theory and the stochastic theory, the sufficient synchronization conditions are derived and proved theoretically, which are mainly for the controllers to be designed and the noise intensities. Finally, some numerical examples are presented to demonstrate the effectiveness of the results. © 2013 Elsevier B.V. All rights reserved.


Yang Y.,Jiangnan University | Yang Y.,Nanjing Southeast University | Cao J.,Nanjing Southeast University
Nonlinear Analysis: Real World Applications | Year: 2010

Based on the stability analysis of the impulsive functional differential equation, the exponential synchronization of the complex dynamical network with a coupling delay and impulses is investigated in the paper. The criteria for the exponential synchronization are derived by the geometrical decomposition of network states and linear matrix inequality method. Two examples are given to show the effectiveness of the proposed criteria. © 2009 Elsevier Ltd. All rights reserved.


Yang Y.,Jiangnan University | Yang Y.,Nanjing Southeast University | Cao J.,Nanjing Southeast University
Nonlinear Analysis: Real World Applications | Year: 2010

In this paper, the optimization techniques for solving a class of non-differentiable optimization problems are investigated. The non-differentiable programming is transformed into an equivalent or approximating differentiable programming. Based on Karush-Kuhn-Tucker optimality conditions and projection method, a neural network model is constructed. The proposed neural network is proved to be globally stable in the sense of Lyapunov and can obtain an exact or approximating optimal solution of the original optimization problem. An example shows the effectiveness of the proposed optimization techniques. © 2009.


Zhang C.,Jiangnan University | Zhang Z.,Nanjing Southeast University | Zhang Q.,Jiangnan University
Journal of Constructional Steel Research | Year: 2012

Low-yield-strength steel 100 (LYS100) is widely applied to design a metallic shear panel damper for its high ductility. A low-yield-strength steel shear panel damper (LYSPD) with the maximum shear strain of 70% is developed and verified by static incremental cyclic loading in previous research. In this paper, further research on the performances of the developed LYSPD including the fatigue characteristic is carried out by static and dynamic constant cyclic tests. Four different shear strain amplitudes (20%, 30%, 40% and 50%) are selected in both static and dynamic tests. Two frequencies, 0.5 Hz and 1 Hz, are adopted for each of the four amplitudes in dynamic tests. Large differences such as stress softening, fatigue cycle deterioration, and temperature increase caused by high strain rate and internal friction are observed in dynamic tests. The test results suggest that the seismic performance of the LYSPD may be overestimated by static tests and the dynamic tests are essential to guarantee the reliability of the LYSPD. © 2012 Elsevier Ltd. All rights reserved.


Luan X.,Jiangnan University | Shi P.,University of South Wales | Shi P.,Victoria University of Melbourne | Liu F.,Jiangnan University
International Journal of Robust and Nonlinear Control | Year: 2011

This paper presents a general framework for robust adaptive neural network (NN)-based feedback linearization controller design for greenhouse climate system. The controller is based on the well-known feedback linearization, combined with radial basis functions NNs, which allows the feedback linearization technique to be used in an adaptive way. In addition, a robust sliding mode control is incorporated to deal with the bounded disturbances and the approximation errors of NNs. As a result, an inherently nonlinear robust adaptive control law is obtained, which not only provides fast and accurate tracking of varying set-points, but also guarantees asymptotic tracking even if there are inherent approximation errors. Copyright © 2010 John Wiley & Sons, Ltd.


Gao H.,Tsinghua University | Xu W.,Jiangnan University
IEEE Transactions on Systems, Man, and Cybernetics, Part B: Cybernetics | Year: 2011

Particle swarm optimization (PSO) is a population-based optimization technique that can be applied to a wide range of problems. Here, we first investigate the behavior of particles in the PSO using a Monte Carlo method. The results reveal the essence of the trajectory of particles during iterations and the reasons why the PSO lacks a global search ability in the last stage of iterations. Then, we report a novel PSO with a moderate-random-search strategy (MRPSO), which enhances the ability of particles to explore the solution spaces more effectively and increases their convergence rates. Furthermore, a new mutation strategy is used, which makes it easier for particles in hybrid MRPSO (HMRPSO) to find the global optimum and which also seeks a balance between the exploration of new regions and the exploitation of the already sampled regions in the solution spaces. Thirteen benchmark functions are employed to test the performance of the HMRPSO. The results show that the new PSO algorithm performs much better than other PSO algorithms for each multimodal and unimodal function. Furthermore, compared with recent evolutionary algorithms, experimental results empirically demonstrate that the proposed framework yields promising search performance. © 2011 IEEE.


Gao H.,Tsinghua University | Gao H.,Jiangnan University | Xu W.,Jiangnan University
Applied Soft Computing Journal | Year: 2011

A new particle swarm optimization (PSO) that incorporates a hybrid mutation strategy is proposed. In this paper we first use the Monte Carlo method to investigate the behavior of the particle in PSO. The results reveal the essence of the particle's trajectory during executions and the reasons why PSO has relative poor global searching ability especially in the last stage of evolution. Then we present a new hybrid particle swarm optimization which incorporates Henon map mutation operation (HPSO) so as to enhance the achievement of PSO. The new mutation strategy divides the mutation operator into global and local mutation operators, then it enables the particles to have stronger exploration ability and fast convergence rate. Sixteen benchmark functions are used to test the performance of HPSO. The results show that the new PSO algorithm performs better than the other hybrid PSO algorithms for each of the test functions. Meanwhile, HPSO is applied to a practical problem (i.e., the economic dispatch problem in a power system) with a satisfying result. © 2011 Elsevier B.V. All rights reserved.


Ji W.,Guangdong Ocean University | Yao W.,Jiangnan University
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2015

Chloramphenicol (CAP) is a widely used amide alcohol antibiotics, which has been banned from using in food producing animals in many countries. In this study, surface enhanced Raman scattering (SERS) coupled with gold colloidal nanoparticles was used for the rapid analysis of CAP. Density functional theory (DFT) calculations were conducted with Gaussian 03 at the B3LYP level using the 3-21G(d) and 6-31G(d) basis sets to analyze the assignment of vibrations. Affirmatively, the theoretical Raman spectrum of CAP was in complete agreement with the experimental spectrum. They both exhibited three strong peaks characteristic of CAP at 1104 cm-1, 1344 cm-1, 1596 cm-1, which were used for rapid qualitative analysis of CAP residues in food samples. The use of SERS as a method for the measurements of CAP was explored by comparing use of different solvents, gold colloidal nanoparticles concentration and absorption time. The method of the detection limit was determined as 0.1 μg/mL using optimum conditions. The Raman peak at 1344 cm-1 was used as the index for quantitative analysis of CAP in food samples, with a linear correlation of R2 = 0.9802. Quantitative analysis of CAP residues in foods revealed that the SERS technique with gold colloidal nanoparticles was sensitive and of a good stability and linear correlation, and suited for rapid analysis of CAP residue in a variety of food samples. © 2015 Elsevier B.V.


Wang Z.-W.,University of Jinan | Jun Wang,Jiangnan University
JVC/Journal of Vibration and Control | Year: 2011

An inverse substructure method is developed for the analysis of the dynamical characteristics of a three-substructures coupled system based on the inverse substructure method of a two-substructures coupled system. The unknown substructure-level frequency response functions (FRFs) and coupling stiffness can be predicted, and the system-level response of key components can be obtained by using the method when the system-level FRFs needed are all measured. To verify the validity of the inverse substructure method of a three-substructures coupled system, a lumped mass-spring-damper model with three substructures coupled is taken as an example for numerical validation. The prediction is exactly the same as the ''Given''. To further check out the accuracy of the method, the experiment of a physical prototype is carried out, and the predicted substructure-level FRFs are in good agreement with those that are measured. The method is applied to the analysis of a product-transport- system, which is treated as a coupled system composed of a key component, a product and a vehicle. The effect of the coupling stiffness on the dynamic response of key components is investigated. The result indicates that reducing the coupling stiffness of the product-vehicle interface can largely lower the response of key components. © The Author(s) 2010.


Wang C.,Jiangnan University | Wang C.,Nanjing University of Technology | Xu L.,Jilin University | Cheng H.,Jiangnan University | And 2 more authors.
Nanoscale | Year: 2014

The development of functional copper nanoclusters (Cu NCs) is becoming increasingly widespread in consumer technologies due to their applications in cellular imaging and catalysis. Herein, we report a simple protein-directed synthesis of stable, water-soluble and fluorescent Cu NCs, using BSA as the stabilising agent. Meanwhile, in this study, hydrazine hydrate (N 2H4·2H2O) was used as the reducing agent. N2H4·2H2O was a mild reducing agent suggesting that all processes could be operated at room temperature. The as-prepared Cu NCs showed red fluorescence with a peaking center at 620 nm (quantum yield 4.1%). The fluorescence of the as-prepared BSA-Cu NCs was responsive to pH in that the intensity of fluorescence increased rapidly by decreasing the pH from 12 to 6. Besides, with an arresting set of features including water-dispersibility, red fluorescence, good biocompatibility, surface-bioactivity and small size, the resultant BSA-Cu NCs could be used as probes for cellular imaging and catalysis. In this study, CAL-27 cells and the reaction of oxidation of styrene are used as models to achieve fluorescence imaging and elucidate the catalytic activity of the as-prepared BSA-Cu NCs. © 2013 The Royal Society of Chemistry.


Ni C.,Jiangnan University | Wu G.,Jiangnan University | Zhu C.,Hohai University | Yao B.,Jiangnan University
Journal of Physical Chemistry C | Year: 2010

Amphiphilic star-shaped block copolymers of poly(ε-caprolactone-star- N-isopropylacrylamide) were synthesized by using hydroxyl-functionalized polyhedral oligomeric silsesquioxanes (POSS) as a core to initiate ring-opening polymerization of ε-caprolactone, followed by atom transfer radical polymerization of N-isopropylacrylamide. The sizes of the core and shell could be controlled through adjusting feed ratios of the reactants. The structure of the star block copolymers were confirmed by FTIR, 1HNMR, 29SiNMR, and DSC. Nano micelles were formed by adding water to the copolymer solution (DMF). The critical water concentration for the formation of the micelles were determined by UV. The results of TEM and DLS showed that the micelles were well-defined three-dimensional spherical particles with an average diameter of 155 nm. A hydrophobic drug ibuprofen could be loaded on the micelles effectively, and the release behavior was temperature dependent. © 2010 American Chemical Society.


Zheng H.,Dalian Polytechnic University | Zheng L.,Jiangnan University
Fibers and Polymers | Year: 2014

Dyeing characteristics of meta-aramid fibers were investigated in supercritical carbon dioxide by employing three disperse dyes and a carrier. The effects of dyeing temperature, pressure, time, dye concentration, CO2 flow, and carrier concentration on dyeing properties were investigated. The results showed that meta-aramid fiber could be dyed in supercritical carbon dioxide. Its color depth was improved with increasing dyeing temperature, pressure, time, dye concentration, CO2 flow, and carrier concentration. Moreover, the color depth could be significantly improved by adding the carrier. The dyeing procedure of supercritical carbon dioxide fluid did not influence the chemical structure and antistatic properties of the meta-aramid fiber. The maximum decomposition temperature and breaking strength of the dyed meta-aramid fiber are slightly increased. The dyed meta-aramid fiber in supercritical carbon dioxide had good fastness, which was rated at 4-5. © 2014 The Korean Fiber Society and Springer Science+Business Media Dordrecht.


Dai X.,Jiangnan University | Chen A.,Jiangnan University | Chen A.,Anhui Polytechnic University | Bai Z.,Jiangnan University
Scientific Reports | Year: 2014

Exploring the molecular difference among breast cancer subtypes is of crucial importance in understanding its heterogeneity and seeking its effective clinical treatment. For this, several layers of information including immunohistochemical markers and a variety of high-throughput genomics approaches have been intensively used. Here we have explored the intrinsic differences among breast cancer subgroups defined by immunohistochemical expression (IHC) of hormone receptors ER and PR as well as human epidermal growth factor receptor 2 (HER2) using the mRNA and miRNA expression profiles of 115 tumors. A core basal group was further defined by epidermal growth factor receptor and cytokeratin 5/6 IHC expression and compared to triple negative group. A set of differentially expressed genes including 1015 mRNAs and 69 miRNAs was found to distinguish tumor subtypes whose generality was demonstrated using two independent data sets. The network was explored for each subtype and biomass synthesis signaling was found to play an important role in the core basal subgroup. This study contributes to elucidating the intrinsic relations among breast cancer subgroups defined by ER, PR and HER2 expression via integrating mRNA and miRNA expression. The results can avail functional studies of breast cancer with translational potential for clinical use.


Luan X.,Jiangnan University | Liu F.,Jiangnan University | Shi P.,University of South Wales | Shi P.,Victoria University of Melbourne
International Journal of Innovative Computing, Information and Control | Year: 2010

This paper deals with the problem of stochastic optimal control for a class of nonlinear systems subject to Markovian jump parameters. The nonlinearities in the different jump modes are initially parameterized by multilayer neural networks (MNNs), which lead to neural Markovian jump systems. A stochastic neural Lyapunov function (NLF) is used to analyze the stability of the resulting neural control MJSs. Then, based on this stochastic NLF and the neural model, a linear state feedback controller is designed to stabilize the closed-loop nonlinear system and guaranteed an upper bound of the system performance for all admissible approximation errors of the MNNs. The control gains can be derived by solving a set of linear matrix inequalities. Finally, a single link robot arm is demonstrated to show the effectiveness of the proposed design techniques. ICIC International © 2010.


Luan X.,Jiangnan University | Shi P.,University of South Wales | Shi P.,Victoria University of Melbourne | Shi P.,University of South Australia | Liu F.,Jiangnan University
IEEE Transactions on Industrial Electronics | Year: 2011

In this paper, an observer-based stabilizing controller has been designed for networked systems involving both random measurement and actuation delays. The developed control algorithm is suitable for networked systems with any type of delays. By the simultaneous presence of binary random delays and making full use of the delay information in the measurement model and controller design, new and less conservative stabilization conditions for networked control systems are derived. The criterion is formulated in the form of a nonconvex matrix inequality of which a feasible solution can be obtained by solving a minimization problem in terms of linear matrix inequalities. An illustrative example is presented to show the applicability of the proposed design technique. © 2010 IEEE.


Yin Y.,Jiangnan University | Shi P.,University of South Wales | Shi P.,Victoria University of Melbourne | Shi P.,University of South Australia | Liu F.,Jiangnan University
IEEE Transactions on Industrial Electronics | Year: 2011

This paper studies the problem of continuous gain-scheduled robust fault detection (RFD) on a class of time-delay stochastic nonlinear systems with partially known jump rates. By means of gradient linearization procedure, stochastic linear models and filter-based residual signal generators are constructed in the vicinity of selected operating states. Furthermore, in order to guarantee the sensitivity to faults and robustness against unknown inputs, an RFD filter (RFDF) is designed for such linear models by first designing H ∞ filters that minimize the influences of the disturbances and modeling uncertainties and then a new performance index that increases the sensitivity to faults. Subsequently, a sufficient condition on the existence of RFDF is established in terms of linear matrix inequality techniques. Finally, a continuous gain-scheduled approach is employed to design continuous RFDFs on the entire nonlinear jump system. A simulation example is given to illustrate that the proposed RFDF can detect the faults correctly and shortly after the occurrences. © 2011 IEEE.


Yin Y.,Jiangnan University | Shi P.,Victoria University of Melbourne | Shi P.,University of South Australia | Shi P.,University of South Wales | Liu F.,Jiangnan University
Journal of the Franklin Institute | Year: 2011

This paper studies the problem of continuous gain-scheduled PI tracking control on a class of stochastic nonlinear systems subject to partially known jump probabilities and time-varying delays. First, gradient linearization procedure is used to construct model-based linear stochastic systems in the vicinity of selected operating states. Next, based on stochastic Lyapunov stabilization analysis, sufficient conditions for the existence of a PI tracking control are established for each linear model in terms of linear matrix inequalities. Finally, continuous gain-scheduled approach is employed to design continuous nonlinear PI tracking controllers on the entire nonlinear jump system. Simulation example is given to illustrate the effectiveness of the developed design techniques. © 2011 The Franklin Institute © 2011 Published by Elsevier Ltd. on behalf of The Franklin Institute.


Luan X.,Jiangnan University | Liu F.,Jiangnan University | Shi P.,University of South Wales | Shi P.,Victoria University of Melbourne
International Journal of Systems Science | Year: 2011

This article proposes a new design approach for robust finite-time H ∞ control of a class of Markov jump systems with partially known information on the transition jump rates. The system under consideration involves norm-bounded parameter uncertainties and external disturbance. The problems of robust finite-time boundedness and finite-time stabilisation of the underlying systems are considered. Then, a H∞ state feedback controller is designed. Sufficient conditions that consider only the known bounds on the transition jump rates are developed in the form of linear matrix inequalities. A numerical example is included to show the usefulness of the theoretic results obtained. © 2011 Taylor & Francis.


Huang H.,Shanghai Maritime University | Zhu D.,Shanghai Maritime University | Ding F.,Jiangnan University
Journal of Intelligent and Robotic Systems: Theory and Applications | Year: 2014

An integrated multiple autonomous underwater vehicle (multi-AUV) dynamic task assignment and path planning algorithm is proposed by combing the improved self-organizing map (SOM) neural network and a novel velocity synthesis approach. Each target is to be visited by one and only one AUV, and a shortest path between a starting point and the destination is found in the presence of the variable current environment and dynamic targets. Firstly, the SOM neuron network is developed to assign a team of AUVs to achieve multiple target locations in dynamic ocean environment. The working process involves special definition of the rule to select the winner, the computation of the neighborhood function, and the method to update weights. Then, the velocity synthesis approach is applied to plan a shortest path for each AUV to visit the corresponding target in dynamic environment subject to the ocean current being variable and targets being movable.Lastly, to demonstrate the effectiveness of the proposed approach, simulation results are given in this paper. © Springer Science+Business Media Dordrecht 2013.


Liu D.,Jiangnan University | Liang L.,Jiangnan University | Xia W.,Jiangnan University | Regenstein J.M.,Cornell University | Zhou P.,Jiangnan University
Food Chemistry | Year: 2013

The objective of this study was to investigate the effect of superchilling at -3 °C compared with ice storage at 0 °C on the biochemical and physical properties of grass carp fillets. Fillets stored at -3 °C showed significant changes in whiteness, drip loss and textural hardness, while changes in pH, total volatile basic nitrogen and TCA-soluble peptides were slowed down. Partial denaturation of myosin as demonstrated by differential scanning calorimetry differed between fillets stored at -3 and 0 °C in that the transition peak showed a left shoulder at -3 °C and sharpened at 0 °C. Detachments between muscle cells and formation of cracks within cells were accelerated during storage at -3 °C, and from 10 days on, clear spaces between and within cells were observed with the concurrent appearance of white spots on the surface of fillets, suggesting the formation of both extra- and intracellular large ice crystals. © 2013 Elsevier Ltd. All rights reserved.


Liu L.,Jiangnan University | Li J.,Jilin University
Science China: Physics, Mechanics and Astronomy | Year: 2014

The magnetic moment of 2+ 1 state for 10Be are calculated and investigated in terms of single particle orbits for protons and neutrons under the framework of ab initio Monte Carlo shell model method in an emax = 3 model space. The reduced matrix elements of orbital and spin angular momentum are evaluated. It is found that the orientations of orbital angular momentum in different single particle orbits are consistent. Conversely, the orientations of spin in different single particle orbits tend to be chaotic. The nuclear magnetic moment of 2+ 1 state for 10Be is obtained as 1.006 μN and is discussed in regards to the contribution of orbital and spin angular momentum both for protons and neutrons. The corresponding g-factor is also given. © Science China Press and Springer-Verlag Berlin Heidelberg 2014.


Liu H.,Tsinghua University | Zhao F.,Jiangnan University | Mao B.,Jiangnan University | Wen X.,Jiangnan University
Bioresource Technology | Year: 2012

An innovative adsorption/nitrification/denitrification/sludge-hydrolysis wastewater treatment process (ENRS) characterized by carbon source manipulation with a biological adsorption unit and a sludge hydrolysis unit was developed to enhance nitrogen removal and reduce sludge production for municipal wastewater treatment. The system presented good performance in pollutants removal, yielding the effluent with average COD, NH4+-N, TN and TP of 48.5, 0.6, 13.2 and 1.0mg/L, respectively. Sixty percent of the total carbon source in the influent was concentrated and separated by the quick adsorption of activated sludge, providing the possibilities of reusing waste carbon source in the denitrification tank and accumulating nitrobacteria in the nitrification tank. Low temperature of 6-15°C and high hydraulic loading rate of 3.0-15.0m 3/d did not affect NH4+-N removal performance, yielding the NH4+-N of lower 1.0mg/L in the effluent. Furthermore, 50% of the residual sludge in the ENRS system could be transformed into soluble COD (SCOD) by alkaline thermal hydrolysis with temperature of 60°C and pH of 11, and the hydrolyzed carbon could completely substitute methanol as a good quality carbon to support high efficient denitrification. © 2012 Elsevier Ltd.


Ruberu T.P.A.,University of Rochester | Dong Y.,Jiangnan University | Das A.,University of Rochester | Eisenberg R.,University of Rochester
ACS Catalysis | Year: 2015

The present study reports photelectrochemical H2 evolution using a water-solubilized S3-cap-CdSe quantum dot-sensitized NiO as the photocathode and either [Co(bdt)2]- (bdt =1,2-benzenedithiolate) or Ni(DHLA)x (DHLA= the anion of dihydrolipoic acid) complex as the H2-forming catalyst. The NiO-S3-cap-CdSe/[Co(bdt)2]- system produces H2 with a turnover frequency of 3000 per CdSe mol·h. Faradaic efficiency for this system is essentially quantitative. Both systems are stable for more than 16 h. (Chemical Presented). © 2015 American Chemical Society.


Nikoo M.,Jiangnan University | Benjakul S.,Prince of Songkla University | Xu X.,Jiangnan University
Food Chemistry | Year: 2015

Antioxidant and cryoprotective effects of Amur sturgeon skin gelatin hydrolysates prepared using different commercial proteases in unwashed fish mince were investigated. Gelatin hydrolysates prepared using either Alcalase or Flavourzyme, were effective in preventing lipid oxidation as evidenced by the lower thiobarbituric acid-reactive substances formation. Gelatin hydrolysates were able to retard protein oxidation as indicated by the retarded protein carbonyl formation and lower loss in sulfhydryl content. In the presence of gelatin hydrolysates, unwashed mince had higher transition temperature of myosin and higher enthalpy of myosin and actin as determined by differential scanning calorimetry. Based on low field proton nuclear magnetic resonance analysis, gelatin hydrolysates prevented the displacement of water molecules between the different compartments, thus stabilizing the water associated with myofibrils in unwashed mince induced by repeated freeze-thawing. Oligopeptides in gelatin hydrolysates more likely contributed to the cryoprotective effect. Thus, gelatin hydrolysate could act as both antioxidant and cryoprotectant in unwashed fish mince. © 2015 Elsevier Ltd. All rights reserved.


Luan X.,Jiangnan University | Liu F.,Jiangnan University | Shi P.,University of South Wales | Shi P.,Victoria University of Melbourne
IET Control Theory and Applications | Year: 2010

This study is concerned with the problem of robust finite-time filtering for a class of non-linear Markov jump systems (MJSs) with partially known information on the transition jump rates. The non-linearities in the system are parameterised by multilayer neural networks. Our attention is focused on the design of a mode-dependent full-order H∞ filter to ensure the finite-time boundedness of the filtering error system and a prescribed H ∞ attenuation level for all admissible uncertainties and approximation errors of the networks. Sufficient conditions of filtering design are developed in terms of solvability of a set of linear matrix inequalities. A tunnel diode circuit is used to show the effectiveness and potentials of the proposed techniques. © 2010 The Institution of Engineering and Technology.


Gu G.,Jiangnan University | Gu G.,Louisiana State University | Marinovici L.,Louisiana State University | Lewis F.L.,University of Texas at Arlington
IEEE Transactions on Automatic Control | Year: 2012

This technical note investigates the problems when the discrete-time multiagent dynamic systems are consensusable, and how to synthesize the consensus feedback control law when the consensusability condition holds. We focus on multiagent systems (MASs) over the undirected graph of the communication topology and derive the consensusability condition for single-input dynamic agent systems under state feedback. By introducing a properly designed dynamic filter into the local control protocols, the consensusability condition can be relaxed. A design procedure is given for the filter and an example shows that it allows consensus whereas standard state feedback protocols do not. © 2012 IEEE.


Ding Z.,Northumbria University | Xu M.,Tsinghua University | Lu J.,Tsinghua University | Liu F.,Jiangnan University
IEEE Transactions on Vehicular Technology | Year: 2012

In this paper, we study how to improve wireless security for bidirectional communications. For the scenario with single-antenna nodes, effective criteria for relay and jamming node selection are developed to ensure that the probability to have nonzero secrecy rates is approaching one, given sufficient relays. For the scenario with multiple-antenna nodes, effective precoding is proposed to ensure that the mutual information obtained at the eavesdropper decreases to zero when there is a sufficient number of relays. © 1967-2012 IEEE.


Liu D.,Jiangnan University | Liang L.,Jiangnan University | Regenstein J.M.,Cornell University | Zhou P.,Jiangnan University
Food Chemistry | Year: 2012

The objective of this study was to extract and characterise pepsin-solubilised collagens (PSC) from the fins, scales, skins, bones and swim bladders of bighead carp and to provide a simultaneous comparison of five different sources from one species. The PSC were mainly characterised as type I collagen, containing two α-chains, and each maintained their triple helical structure well. The thermostability of PSC from the internal tissues (swim bladders and bones) was slightly higher than that of PSC from the external tissues (fins, scales and skins). The peptide hydrolysis patterns of all PSC digests using the V8 protease were similar. All PSC were soluble at acidic pH (1-6) and lost their solubility at NaCl concentrations above 30 g/l. The resulting PSC from the five tissues would all be potentially useful commercially. © 2012 Elsevier Ltd. All rights reserved.


Wang D.-Q.,Qingdao University | Ding F.,Jiangnan University | Zhu D.-Q.,Shanghai Maritime University
International Journal of Control, Automation and Systems | Year: 2013

This paper focuses on the identification problem of multivariable controlled autoregressive autoregressive (CARAR-like) systems. The corresponding identification model contains a parameter vector and a parameter matrix, and thus the conventional least squares methods cannot be applied to directly estimate the parameters of the systems. By using the hierarchical identification principle, this paper presents a hierarchical generalized least squares algorithm and a filtering based hierarchical least squares algorithm for the multivariable CARAR-like systems. The simulation results show that the two hierarchical least squares algorithms are effective. © 2013 Institute of Control, Robotics and Systems and The Korean Institute of Electrical Engineers and Springer-Verlag Berlin Heidelberg.


Wang Z.-W.,University of Jinan | E Y.P.,University of Jinan | E Y.P.,Jiangnan University
Materials and Design | Year: 2010

A mathematical model was developed to describe the relationship between the energy absorption properties of paper honeycombs and ambient humidity, as well as the structural parameters thereof. The model is a piecewise function modelling the energy absorption of four deformation stages of paper honeycomb (linear-elastic stage, yield stage, plateau stage and densification stage) separately. Function of each stage is a simple formula relating the energy absorption capacity to the thickness-to-length ratio of honeycomb cell, the mechanical property of a cell-wall material tested under a controlled atmosphere [23°C and 50% relative humidity (RH)] and the RH. Energy absorption curves were thereby obtained for paper honeycombs with a wide range of thickness-to-length ratios in arbitrary humidity environments. The created model was then verified by comparing the predicted energy absorption curves with the experimental ones. A good accordance between the predictions and the observations was achieved, indicating that the energy absorption models developed here could be used to practical application for the designing optimisation and material selection of paper honeycombs. © 2010 Elsevier Ltd.


News Article | November 10, 2016
Site: www.gizmag.com

The scientists obtained melanin from cuttlefish, which manipulate the pigment to alter their appearance(Credit: londondeposit/ Depositphotos ) If you want longer-lasting plastic foams or textiles, the answer may lie in your skin, hair and eyes. All of those body parts get their color from a naturally-occurring pigment known as melanin, which is also found in other animals. Now, scientists from China's Jiangnan University have determined that even a small amount of added melanin makes polyurethane much stronger. In lab tests, a team led by Mingqing Chen and Weifu Dong started by extracting melanin from ink sacs of cuttlefish and blending it into ordinary liquid polyurethane. Even when the resulting cured foam contained as little as two percent melanin, it still exhibited about a 10-fold increase in both toughness (impact-resistance) and tensile strength (stretchability). Whereas unaltered polyurethane could stretch by 770 percent before breaking, the foam with added melanin was able to get to 1,880 percent. According to the scientists, this increase was at least partly due to the melanin nanoparticles' tendency to link with the polymer chains that make up the polyurethane. A paper on the research was recently published in the journal Biomacromolecules.


Chen Y.,University of Hohenheim | Chen Y.,Jiangnan University | Schwack W.,University of Hohenheim
Journal of Chromatography A | Year: 2014

The world-wide usage and partly abuse of veterinary antibiotics resulted in a pressing need to control residues in animal-derived foods. Large-scale screening for residues of antibiotics is typically performed by microbial agar diffusion tests. This work employing high-performance thin-layer chromatography (HPTLC) combined with bioautography and electrospray ionization mass spectrometry introduces a rapid and efficient method for a multi-class screening of antibiotic residues. The viability of the bioluminescent bacterium Aliivibrio fischeri to the studied antibiotics (16 species of 5 groups) was optimized on amino plates, enabling detection sensitivity down to the strictest maximum residue limits. The HPTLC method was developed not to separate the individual antibiotics, but for cleanup of sample extracts. The studied antibiotics either remained at the start zones (tetracyclines, aminoglycosides, fluoroquinolones, and macrolides) or migrated into the front (amphenicols), while interfering co-extracted matrix compounds were dispersed at hRf 20-80. Only after a few hours, the multi-sample plate image clearly revealed the presence or absence of antibiotic residues. Moreover, molecular information as to the suspected findings was rapidly achieved by HPTLC-mass spectrometry. Showing remarkable sensitivity and matrix-tolerance, the established method was successfully applied to milk and kidney samples. © 2014 Elsevier B.V.


Xiao Y.,Jiangnan University
Chinese Control Conference, CCC | Year: 2013

For a class of discrete time dynamical systems, an adaptive control scheme is proposed based on neural networks and multi-model. By designing a reasonable switching law among the models, the merits of linear robust adaptive controller and a neural networks based nonlinear adaptive controller can be well integrated, such that the best controller can be selected for the system at anytime. The control of stability and performance improving can achieve respectively, which not only guarantees the stability, but also improves the adaptive control performance by using neural network controller. Finally, it is demonstrated that improved performance and stability can be simultaneously achieved by simulation examples. © 2013 TCCT, CAA.


Wang Z.,Jiangnan University | Xu H.,Jiangnan University | Wu J.,Jiangnan University | Ye J.,Jiangnan University | Yang Z.,Jiangnan University
Food Chemistry | Year: 2011

A sensitive and specific method for detecting foodborne pathogen, with Salmonella as model analyte, has been developed basing upon DNA hybridization and fluorescent bioconjugated nanoparticles probe. The indicator probe was designed through bioconjugating the Salmonella sequence-specific oligonucleotide with the fluorescent Ru(bpy)32+-doped silica nanoparticles that were prepared by a microemulsion method. Through a sandwich-type DNA hybridization procedure, the target Salmonella DNA was captured and the indicator probe was assembled onto solid matrix so that the Salmonella DNA can be measured by the fluorescent signals of assembled indicator probes. Under optimal conditions, the calibration graph for detection of target Salmonella DNA is linear over the range of 10-10,000. fmol/L with a detection limit of 3. fmol/L. The proposed method was also applied to detect Salmonella in mixed bacteria sample. It gives the detection limit of 30. cfu/mL. The method offers a potential resolution for simple, rapid and sensitive detection of foodborne pathogens in food, clinical and environmental samples. © 2010 Elsevier Ltd.


Ruan Q.,Jiangnan University | Chen Y.,Jiangnan University | Kong X.,Jiangnan University | Hua Y.,Jiangnan University
Journal of Agricultural and Food Chemistry | Year: 2013

In this study, the sulfhydryl (SH) contents of unheated and heated (90 C, 5 min) soy protein were detected under different conditions (pH, reagent addition order, SDS/GuHCl concentration, EDTA) using two aromatic disulfide reagents: 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) and 4,4′-dithiodipyridine (DPS). Two fluorescent alkylating reagents, monobromobimane (mBBr) and N-(1-pyrenyl)maleimide (NPM), were chosen due to their high sensitivity and were also used. Amino acid analysis was used to detect the SH (cysteine) contents of unheated (7.51 ± 0.45 μmol SH/g protein) and heated (1.47 ± 0.10 μmol SH/g protein) soy protein, and similar results were obtained using enzymatic hydrolysis-assisted DPS. The SH content detected by DTNB was affected by pH, denaturant species, and denaturant concentration, and the best results were obtained at pH 7.0 when 6 M GuHCl was added after DTNB. These results were lower than that of the amino acid analysis, however. The SH detected by DPS was not as affected as that of DTNB by pH, denaturant species, and denaturant concentration. Additionally, the results of the amino acid analysis were similar to that of DPS at pH 7.0 in 2% SDS and 4-6 M GuHCl when SDS and GuHCl were added after DPS. EDTA did not have a significant effect on SH detection when DTNB and DPS were added before SDS and GuHCl. Finally, although mBBr and NPM can detect SH in low protein concentrations (1/10 of that required for DTNB and DPS), mBBr and NPM overestimated the SH content of soy protein. Therefore, using DPS at pH 7.0 when it is added before SDS and GuHCl is the most reliable method for detecting the SH content of soy protein. © 2013 American Chemical Society.


Duan F.,Jiangnan University | Zheng Y.,Jiangnan University | Chen M.,Jiangnan University
Applied Surface Science | Year: 2011

Flowerlike PtCl4/Bi2WO6 composite photocatalyst was successfully synthesized through a simple two-step method involving a template-free hydrothermal process and the following impregnation treatment. The samples were fully characterized by the study of X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), X-ray photoelectron spectroscopy (XPS), and UV-Vis absorption spectra. The results indicated that the doping of Pt species did not affect the crystal structure and the morphology of Bi2WO6 photocatalyst, but it had great influences on the photocatalytic activity of Bi2WO6 towards rhodamine-B (RhB) degradation. Besides, the Pt species was found to be present as PtCl4 in the composite samples, and also an optimal Pt species content on the surface of Bi2WO6 photocatalyst was discovered with the highest photocatalytic ability. The improved photocatalytic performance could be ascribed to the enhanced interfacial charge transfer and the inhibited recombination of electron-hole pairs. Meanwhile, a possible mechanism for RhB photocatalytic degradation over PtCl4/Bi 2WO6 catalyst was also proposed. © 2010 Elsevier B.V. All rights reserved.


Fan L.,Jiangnan University | Ding S.,Jiangnan University | Deng K.,Jiangnan University
Carbohydrate Polymers | Year: 2012

The medicinal mushroom Inonotus obliquus has been used as a folk remedy for a long time in Russia and East-European countries to treat gastrointestinal cancer, cardiovascular disease and diabetes. In our study, a water-soluble polysaccharide (ISP2a) was successfully purified from I. obliquus by DEAE-Sepharose CL-6B and Sepharose CL-6B column chromatography. In vivo ISP2a had not only shown antitumor activity, but also could significantly enhance the immune response of tumor-bearing mice. In addition, ISP2a significantly enhanced the lymphocyte proliferation and increased the production of TNF-α. Results of these studies demonstrated that ISP2a had a potential application as natural antitumor agent with immunomodulatory activity. © 2012 Elsevier Ltd. All rights reserved.


Ding Z.-D.,Jiangnan University | Shi J.-C.,Jiangnan University | Xiao J.-J.,Jiangnan University | Gu W.-X.,Jiangnan University | And 2 more authors.
Carbohydrate Polymers | Year: 2012

Efficient catalytic conversion of microcrystalline cellulose (MCC) to 5-hydroxymethyl furfural (HMF), is achieved using acidic ionic liquids (ILs) as the catalysts and metal salts as co-catalysts in the solvent of 1-ethyl-3-methylimidazo-lium acetate ([emim][Ac]). A series of acidic ILs has been synthesized and tested in conversion of MCC to HMF. The effect of reaction conditions, such as reaction time, temperature, catalyst dosage, metal salts, water dosage, Cu2+ concentration and various acidic ILs are investigated in detail. The results show that CuCl2 in 1-(4-sulfonic acid) butyl-3-methylimidazolium methyl sulfate ([C4SO 3Hmim][CH3SO3]), is found to be an efficient catalyst for catalytic conversion of MCC to HMF, and 69.7% yield of HMF is obtained. A mechanism to explain the high activity of CuCl2 in [C4SO3Hmim][CH3SO3] is proposed. To the best of our knowledge, this report first proposes that the Cu2+ and [C4SO3Hmim][CH3SO3] show better catalytic performance in catalytic conversion of MCC to HMF. © 2012 Elsevier Ltd. All rights reserved.


Jiang J.,Jiangnan University | Gou C.,Jiangnan University | Luo J.,Jiangnan University | Yi C.,Jiangnan University | Liu X.,Jiangnan University
Inorganic Chemistry Communications | Year: 2012

A new coumarin based colorimetric nickel sensor 1 with high selectivity and sensitivity toward Ni2+ ion was reported. The absorption maximum of 1 shows a large red shift from 341 nm to 540 nm (Δ = 200 nm) in the presence of Ni2+ ion, and the resulted color change from colorless to pink upon addition of Ni2+ is very easily observed by the naked eye, while other metal ions, such as Zn2+, Cd2+, Cu 2+, Fe3+, Ag+, Pb2+, alkali metal and alkaline earth metal cations do not induce such a change. By the "naked eye", the detection limit of Ni2+ is as low as 0.5 μM in solution, which is lower than the current American Environmental Protection Agency (EPA) standard for drinking water. As far as we are aware of, this is the first reported colorimetric chemosensor capable of detecting Ni2+ ion. © 2011 Elsevier B.V. All rights reserved.


Zhu P.,Changshu Institute of Technology | Xiong W.,Jiangnan University | Xu B.,Jiangnan University
International Journal of Advancements in Computing Technology | Year: 2012

Sensor management is very essential to the multi-sensor system in the target assignment, and become the core element of the information fusion system to improve its performance. To solve the problem effectively, the features of several resolutions for goal assignment and existing questions in the sensor management are analyzed, and then the comprehensive efficiency function is proposed. Combined with an improved PSO algorithm with dynamic weights, a novel algorithm based on an improved PSOAdaptive Weitht PSO (AWPSO) is presented. Finally, the sensor resources' effective assignment is realized under the most superior criterion. Experiments and analysis show that the proposed method clearly reduces the fusion data fluctuation and the error of the sensor management system, especially for the problem of large-scale target assignment. The new method shows faster convergence rate and higher precision.


Yuan X.,Jiangnan University | Zhang S.,Jiangnan University | Ding Y.,Jiangnan University
Inorganic Chemistry Communications | Year: 2012

A novel iridium(III) alkoxide dimeric complex containing 2-(4,6-difluorophenyl)pyridyl cyclometalating ligand was synthesized and characterized by IR, 1H-NMR and X-ray crystallographic methods. Aside from its novel structure and mechanistic implications as intermediate during the formation of homoleptic or heteroleptic Ir(III) complexes, it is interesting to note that this complex shows significant photoluminescence. © 2011 Elsevier B.V. All rights reserved.


Zhang J.,Jiangnan University
Xitong Fangzhen Xuebao / Journal of System Simulation | Year: 2016

A real-time simulation algorithm was proposed to generate a wide range of the common aviation weather condition for the flight simulator based-on a set of dynamic satellite cloud images. With this efficient weather simulation algorithm, a fast radar echo visual simulating method was proposed. The proposed method used the 2D simplified Navier-Stokes and microphysics equations to implement the essential numerical simulation of the aviation weather condition. By this way, the complex problem of large-scale cloud resolving and particle-backscattering computing could be viewed as a simple 2D multi-resolution image processing. Real application on the flight simulator shows that the proposed method can provide a vividly visual airborne weather radar display in real-time on a normal PC platform. © 2016, The Editorial Board of Journal of System Simulation. All right reserved.


He S.,Jiangnan University | He S.,Anhui University | He S.,University of Manchester | Liu F.,Jiangnan University
Signal Processing | Year: 2011

This paper studied the proportional-integral (PI) control problems of stochastic Markovian jump systems (MJSs) with uncertain parameters. Under complete access to the system states, the PI controller design procedure turns to static output feedback control problem that make the closed-loop dynamics of this class of uncertain MJSs be robustly stochastically stable. A sufficient condition on the existence of PI controller is presented and proved by means of linear matrix inequality techniques. The presented results are extended to the case when the system states are not accessible. In order to make the relative equations approximate with a satisfactory precision, we described the problem as a semidefinite programming one via disciplined convex optimization. Simulation results illustrate the validity of the proposed algorithms. © 2011 Elsevier B.V. All rights reserved.


Li X.,Jiangnan University | Xia W.,Jiangnan University
Carbohydrate Polymers | Year: 2010

Chitosans (CHI) with different degree of deacetylation (DD) (60.5%, 65.4%, 70.8%, 77.3%, 86.1%) and different molecular weight (MW) (299, 410, 600, 706, 880 kDa) were obtained. Heat-induced composite gels were prepared from 4% salt-soluble meat proteins (SSMP) with chitosans at 0.6 mol/L NaCl, pH 7.0. Penetration forces and storage modulus of SSMP-CHI composite gels increased proportionally with increasing amount of molecular weight. Gel containing chitosan with DD 77.3% exhibited the highest penetration force and storage modulus. The SEM observations showed that SSMP-CHI incorporated gels were compact and fine compared with that of SSMP. Chemical forces involved in SSMP-CHI composite gels were investigated by determining the effects of NaCl, urea, propylene glycol, and 2-MeSH on penetration forces. Results of these studies demonstrated that interaction between CHI and SSMP contributed to the enhancement of texture of the SSMP-CHI composite gels by electrostatic interactions and hydrogen bonds. © 2010 Elsevier Ltd. All rights reserved.


Gao C.,Jiangnan University | Wu X.-J.,Jiangnan University
Procedia Engineering | Year: 2012

Support vector machine (SVM) not only can be used for classification, can also be applied to regression problems by the introduction of an alternative loss function. Now most of the regress algorithms are based on vector as input, but in many real cases input samples are tensors, support tensor machine (STM) by Cai and He is a typical learning machine for second order tensors. In this paper, we propose an algorithm named kernel support tensor regression (KSTR) using tensors as input for function regression. In this algorithm, after mapping the each row of every original tensor or of every tensor converted from original vector into a high dimensional space, we can get associated points in a new high dimensional feature space, and then compute the regression function. We compare the results of KSTR with the traditional SVR algorithm, and find that KSTR is more effective according to the analysis of the experimental results. © 2011 Published by Elsevier Ltd.


Xu G.,Jiangnan University | Liu L.,Jiangnan University | Chen J.,Jiangnan University
Microbial Cell Factories | Year: 2012

Background: Fumaric acid is a commercially important component of foodstuffs, pharmaceuticals and industrial materials, yet the current methods of production are unsustainable and ecologically destructive.Results: In this study, the fumarate biosynthetic pathway involving reductive reactions of the tricarboxylic acid cycle was exogenously introduced in S. cerevisiae by a series of simple genetic modifications. First, the Rhizopus oryzae genes for malate dehydrogenase (RoMDH) and fumarase (RoFUM1) were heterologously expressed. Then, expression of the endogenous pyruvate carboxylase (PYC2) was up-regulated. The resultant yeast strain, FMME-001 ↑PYC2 + ↑RoMDH, was capable of producing significantly higher yields of fumarate in the glucose medium (3.18 ± 0.15 g liter -1) than the control strain FMME-001 empty vector.Conclusions: The results presented here provide a novel strategy for fumarate biosynthesis, which represents an important advancement in producing high yields of fumarate in a sustainable and ecologically-friendly manner. © 2012 Xu et al; licensee BioMed Central Ltd.


Li L.,Jiangnan University | Wu F.,Jiangnan University | Zhang S.,Jiangnan University | Wang D.,Jiangnan University | And 2 more authors.
Dalton Transactions | Year: 2013

In this paper, a C-F bond activation reaction of a chloro-bridged iridium(iii) dimer (dfppy)2Ir(μ-Cl)2Ir(dfppy) 2 (1) (dfppy denotes 2-(4,6-difluorophenyl)pyridyl) in the presence of sodium methoxide has been reported, leading to the formation of a heteroleptic cyclometalated iridium(iii) fluorophenylpyridine complex 2. HPLC-mass analysis confirmed the release of formaldehyde in the product mixture. When sodium benzyloxide was used as the base, complex 2 was also generated with the release of a benzaldehyde derivative. Complex 2 has been fully characterized by 1H-NMR, 19F-NMR and X-ray crystallographic methods, confirming the partial loss of one of the fluorine atoms on one of the cyclometalated phenylpyridyl ligands. Photophysical studies of complex 2 show that it has a similar absorption spectrum to that of Ir(iii)(dfppy)3. However, the emission spectrum shows a red shift maximum emission band at 478 nm due to the loss of a single fluorine atom, highlighting the critical effect of fluorine on the photoluminescence of these Ir(iii) complexes. Finally, intensive mechanistic studies including HPLC-mass analysis, 1H-NMR, and 19F-NMR studies demonstrate that the formation of complex 2 should involve a critical β-hydride elimination of Ir(iii)-alkoxide intermediate and the participation of Ir-hydride and/or Ir-fluoride intermediates. © The Royal Society of Chemistry 2013.


Ding J.,Jiangnan University | Yang H.,Jiangnan University
Circuits, Systems, and Signal Processing | Year: 2014

This paper addresses the problem of robust adaptive iterative learning control for a chain of uncertain integral nonlinear systems, whose aim is to stabilize the tracking error of the system and improve convergence speed in the presence of uncertainties. In response to unknown bounded disturbances, a continuous second-order sliding mode adaptive iterative learning control scheme is proposed, in which an integral term is to attenuate the effects of the disturbances and achieve fast convergence performance. By designing a suitable controller and composite energy function, it is proved that the tracking error along iterative learning horizon will converge to a small neighborhood of zero. Numerical examples are provided to validate the efficacy of the proposed method. © 2014 Springer Science+Business Media New York.


Li H.,Jiangnan University | Jin Z.,Jiangnan University | Xu X.,Jiangnan University
Food Control | Year: 2013

Chinese rice wine, produced by simultaneous saccharification and fermentation (SSF), has an uneconomic, time-consuming and environment-unfriendly pretreatment. In this study, an enzymatic extrusion pretreatment was applied to manufacture Chinese rice wine. Response surface methodology (RSM) was used to investigate the effect of process parameters namely barrel temperature (BT), moisture content (MC), and α-amylase concentration (AC) on the alcoholic degree of rice wine. Statistical analyses revealed that the experimental data were adequately fitted into the second-order polynomial model with a high coefficient of determination (R2) of 0.97. It also showed that MC had the most significant effect on the alcoholic degree among the three parameters. Starch molecular weight distribution data indicated that the alcoholic degree was closely related to the oligosaccharides forming during enzymatic extrusion. Total of amino acids (AA) in the extrusion-processed wine was 41% higher than that in the traditional one. These results suggest that the enzymatic extrusion pretreatment, instead of the traditional one, is feasible for Chinese rice wine production. © 2013 Elsevier Ltd.


Liu X.,Zhejiang University | Lu J.,Jiangnan University
Automatica | Year: 2010

This paper studies modeling and identification problems for multi-input multirate systems with colored noises. The state-space models are derived for the systems with different input updating periods and furthermore the corresponding transfer functions are obtained. To solve the difficulty of identification models with unmeasurable noises terms, the least squares based iterative algorithm is presented by replacing the unmeasurable variables with their iterative estimates. Finally, the simulation results indicate that the proposed iterative algorithm has advantages over the recursive algorithms. © 2010 Elsevier Ltd. All rights reserved.


Gao G.,Jiangnan University
Advanced Materials Research | Year: 2014

To explore new ideas for treating sludge in the urban rivers, 4 different sludge treatment schemes were proposed, the construction safety, projection duration, enthusiasm of the public, environmental coordination, resource utilization, supporting of government, benefits, and project cost of the schemes were selected as the main evaluation indexes, and the projection pursuit model was introduced to evaluate the schemes comprehensively. Results showed that: the evaluation and selection of sludge treatment schemes conformed to the calculation requirements of the projection pursuit model; S3 was the optimal sludge treatment scheme with best comprehensive benefits, the projection value of which was recorded as 1.198, much more than that of other treatments. Therefore, it was concluded that using sludge to produce brick (S3) was more appropriate for the sludge treatment in urban rivers, and which depended on the wide market of bricks and had good practical benefits. The study achievements could provide beneficial basis for the river management and the sludge treatment in cities, and also provide the practical basis for the application of statistical models on the selection of project schemes. © (2014) Trans Tech Publications, Switzerland.


Ni Y.,Jiangnan University | Zhou J.,Jiangnan University | Sun Z.,Jiangnan University
Process Biochemistry | Year: 2012

(S)-(4-Chlorophenyl)-(pyridin-2-yl)methanol [(S)-CPMA] is an important chiral intermediate of anti-allergic drug Betahistine. Carbonyl reductase-producing microorganisms were isolated from soil samples for the stereoselective reduction of (4-chlorophenyl)-(pyridin-2-yl)methanone (CPMK) to (S)-CPMA. Among over 400 microorganisms isolated, one strain exhibiting the highest activity was selected and identified as Kluyveromyces sp. After optimization, the biotransformation reaction catalyzed by Kluyveromyces sp. CCTCC M2011385 whole-cell gave product (S)-CPMA in 81.5% ee and 87.8% yield at substrate concentration of 2 g/L in aqueous phase. Using an aqueous two-phase system (ATPs) consisted of PEG4000 (20%, w/w) and Na 2HPO 4 (14%, w/w), the product reached 86.7% ee and 92.1% yield at a higher substrate concentration of 6 g/L. The substrate tolerance and biocompatibility of microbial cells are greatly improved in ATPs by accumulating substrate/product in the upper PEG solution. This study, for the first time, reports the production of (S)-CPMA catalyzed by microbial cells. © 2012 Elsevier Ltd.


Ding R.,Jiangnan University | Duan H.,Jiangnan University
Proceedings of the American Control Conference | Year: 2012

This paper presents a two-stage recursive least squares (TS-RLS) algorithm for pseudo-linear regressive models corresponding to the Box-Jenkins models by combining the auxiliary model identification idea and the decomposition technique. The basic idea is to decompose a system into two subsystems with the system model parameters and the noise model parameters, respectively, and then to identify the parameters of each subsystem. Compared with the auxiliary model based recursive generalized extended least squares algorithm, the TS-RLS algorithm has less computational burden. The simulation results confirm these conclusions. © 2012 AACC American Automatic Control Council).


Ding F.,Jiangnan University
Proceedings of the American Control Conference | Year: 2012

This paper studies parameter estimation problem of Hammerstein systems by using the gradient search principle. The Hammerstein system is a bilinear-parameter system which is linear about two parameter vectors, respectively. A separable gradient algorithm is developed for estimating the two parameter vectors based on the hierarchical identification principle. The algorithm is simple in principle and easy to implement online. The simulation results test the effectiveness of the proposed algorithm. © 2012 AACC American Automatic Control Council).


Peng L.,Jiangnan University
IFAC-PapersOnLine | Year: 2015

A recursive probabilistic principal component analysis (PPCA) based data-driven fault identification method is proposed to handle the missing data samples and the mode tran-sition in multi-mode process. This model is recursively obtained by using the increasing number of normal observations with partly missing data. First, based on the singular value of historic data matrix, the whole process is divided into different steady modes and mode transitions. For steady modes, the conventional PPCA is used to obtain the principal components, and to impute the missing data. When the mode is a mode transition, the proposed recursive PPCA is applied, which can actually reveal the between-mode dynamics for process monitoring and fault detection. After that, in order to identify the faults, a contribution analysis method is developed and used to identify the variables which make the major contributions to the occurrence of faults. The effectiveness of the proposed approach is demonstrated by the Tennessee Eastman chemical process. The results show that the presented approach can accurately detect abnormal events, identify the faults, and it is also robust to mode transitions. © 2015, (International Federation of Automatic Control) Hosting by Elsevier Ltd. All rights reserved.


Ding F.,Jiangnan University
Proceedings of the 2012 24th Chinese Control and Decision Conference, CCDC 2012 | Year: 2012

By means of the data filtering technique, this presents a two-stage least squares based iterative algorithm for systems with colored noises, i.e., controlled autoregressive autoregressive moving average (CARARMA) systems. The key is to obtain two identification models by using the decomposition technique, one including the parameters of the system model, and the other including the parameters of the noise model. Then we use the least squares principle to interactively estimate the parameters of two submodels. The proposed algorithm has lower computational cost and is effective for estimating the parameters of the CARARMA systems. © 2012 IEEE.


Wang Y.,Jiangnan University | Ding F.,Jiangnan University
IET Control Theory and Applications | Year: 2016

This study applies the filtering technique to system identification to study the data filtering-based parameter estimation methods for multivariable systems, which are corrupted by correlated noise - an autoregressive moving average process. To solve the difficulty that the identification model contains the unmeasurable variables and noise terms in the information matrix, the authors present a hierarchical gradient-based iterative (HGI) algorithm by using the hierarchical identification principle. To improve the convergence rate, they apply the filtering technique to derive a filtering-based HGI algorithm and a filtering-based hierarchical least squares-based iterative (HLSI) algorithm. The simulation examples indicate that the filtering-based HLSI algorithm has the highest computational efficiency among these three algorithms. © The Institution of Engineering and Technology 2016.


Wang L.,Jiangnan University | Zhu Y.,Jiangnan University | Xu L.,Jiangnan University | Chen W.,Jiangnan University | And 5 more authors.
Angewandte Chemie - International Edition | Year: 2010

(Figure Presented) Parallel or angle parked: Cold nanorods (see picture) were selectively modified either on the sides or ends using complementary microcystin (MC-LR) antibody and antigen (blue). Fast detection of MC-LR (green) was successfully achieved with these assemblies, and both sensitivity and detection ranges were markedly better for the end-to-end motif (right) than the sideto-side variant (left). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.


Ji X.,Jiangnan University | Su H.,Zhejiang University
IEEE Transactions on Automatic Control | Year: 2016

Petersen's lemma on matrix uncertainty is commonly used in the analysis and design for uncertain systems with time-varying norm-bounded uncertain parameter. In this note, Petersen's lemma is extended to the case where the uncertain inequality contains two uncertain matrix pairs. The condition is given such that the Petersen's lemma gives a necessary and sufficient condition. A numerical example is given to show the effectiveness of the proposed method. © 1963-2012 IEEE.


Lu X.-F.,Jiangnan University | Lu X.-F.,Central South University | Xiao P.,Central South University
Carbon | Year: 2013

Silicon carbide nanofibers (SiCNFs) used as the second reinforcements of carbon/carbon composites were grown radially on the carbon fiber surface. The microstructure of SiCNFs and their effects on the microstructure and flexural properties of C/C composites were investigated. Results show that there are many defects such as twin crystals and stacking faults in SiCNFs which were grown by catalytic chemical vapor deposition. During the same process, the skin region of carbon fiber has changed. Several SiC layers are formed and the arrangement of the graphite layers around SiC layers is more orderly. In the next chemical vapor infiltration, due to the induction of SiCNFs, the middle textural pyrocarbon were formed firstly and then is the high textural pyrocarbon. The existence of SiCNFs also contributes to the three-phase interface between pyrocarbon, SiCNFs and carbon fibers, resulting in a good bond between carbon fiber and matrix. Those structural changes lead the better flexural properties of SiCNF-C/C composites compared with C/C composites. © 2013 Elsevier Ltd.


Shen X.,Jiangnan University
Micro and Nano Letters | Year: 2012

Highly luminescent and water-soluble CdTe quantum dots encapsulated in polyvinylpyrrolidone nanoparticles (QDs/PNs) have been synthesised by one-pot aqueous route. Fluorescence of the as-prepared QDs/PNs was tunable from 539 to 606nm and photoluminescent quantum yield could reach up to 47. High quality of QDs/PNs was also observed by narrow full-width at half-maximum as small as 36nm in the fluorescence spectra. Using fluorescence microscopy, we demonstrated uptake of QDs/PNs by 293T human renal epithelial cells. Our results suggest that such nanoparticles are potentially powerful tools for fluorescent gene delivery reagent. © 2012 The Institution of Engineering and Technology.


Zheng Y.,Jiangnan University | Duan F.,Jiangnan University | Chen M.,Jiangnan University | Xie Y.,Jiangnan University
Journal of Molecular Catalysis A: Chemical | Year: 2010

In this work a novel Bi2O2CO3 photocatalyst was first put forward by virtue of structural understanding. In particular, the flower-like Bi2O2CO3 hierarchitecture with the controlled special {0 0 1} plane exposed was first synthesized via a mild route, showing the excellent photocatalytic activity due to the large distortion of Bi-O on the exposed surface. The Bi2O2CO3 nanostructures have proved to be not only a new photocatalyst under solar light irradiation, but also a possible example for investigating the key factors of which may impact on the photocatalytic ability. The results confirmed that the higher efficiency of the photocatalytic activity should be contributed synergistically by the higher BET surface area and the special exposed surface. © 2009.


Zhang M.,Jiangnan University | Jiang H.,Jiangnan University | Lim R.-X.,Jiangnan University
Drying Technology | Year: 2010

This article provides a capsule summary of very recent R&D carried out in the Food Sciences Key Laboratory of Jiangnan University, Wuxi, China. It focuses on a comparative experimental evaluation of diverse drying techniques ranging from traditional hot air drying, vacuum drying, and freeze drying to microwave vacuum drying and microwave freeze drying of fruits, vegetables, and aquatic products. More recently studies were also made on drying of extruded restructured chips of mixed composition, that is, potato blended with apple, which could be a novel snack food of the future. Drying kinetics as well as various key quality parameters, for example, color, texture, nutritional value, crispness, etc., were measured along with close examination of the microstructure that develops differently under different drying conditions. The potential value of microwave assistance in drying of foods in industrial scale applications is explored. © 2010 Taylor & Francis Group, LLC.


Yin L.,Jiangnan University | Hu X.,Jiangnan University | Xu D.,Agricultural University of Hebei | Ning J.,Jinghai Amino Acid Company | And 2 more authors.
Metabolic Engineering | Year: 2012

Threonine dehydratase and acetohydroxy acid synthase are critical enzymes in the l-isoleucine biosynthesis pathway of Corynebacterium glutamicum, but their activities are usually feedback-inhibited. In this study, we characterized a feedback-resistant threonine dehydratase and an acetohydroxy acid synthase from an l-isoleucine producing strain C. glutamicum JHI3-156. Sequence analysis showed that there was only a single amino acid substitution (Phe383Val) in the feedback-resistant threonine dehydratase, and there were three mutated amino acids (Pro176Ser, Asp426Glu, and Leu575Trp) in the big subunit of feedback-resistant acetohydroxy acid synthase. The mutated threonine dehydratase over-expressed in E. coli not only showed completely resistance to l-isoleucine inhibition, but also showed enhanced activity. The mutated acetohydroxy acid synthase over-expressed in E. coli showed more resistance to l-isoleucine inhibition than the wild type. Over-expression of the feedback-resistant threonine dehydratase or acetohydroxy acid synthase in C. glutamicum JHI3-156 led to increase of l-isoleucine production; co-expression of them in C. glutamicum JHI3-156 led to 131.7% increase in flask cultivation, and could produce 30.7. g/L l-isoleucine in 72-h fed-batch fermentation. These results would be useful to enhance l-isoleucine production in C. glutamicum. © 2012 Elsevier Inc.


Wang D.,Qingdao University | Yang G.,Qingdao University | Ding R.,Jiangnan University
Computers and Mathematics with Applications | Year: 2010

This paper presents a gradient-based iterative identification algorithms for Box-Jenkins systems with finite measurement input/output data. Compared with the pseudo-linear regression stochastic gradient approach, the proposed algorithm updates the parameter estimation using all the available data at each iterative computation (at each iteration), and thus can produce highly accurate parameter estimation. An example is given. © 2010 Elsevier Ltd. All rights reserved.


Chen W.,Shenyang Ligong University | Qu B.,Jiangnan University
International Journal of Hydrogen Energy | Year: 2010

A hydrogen peroxide-treated carbon support was used to prepare electrocatalysts for low temperature fuel cells. The electrochemical stability of the Pt/C catalysts was evaluated by potential scan tests. The results show that the oxidative treatment introduces oxygen-bearing functional groups onto the support surface. These functional groups connect with metal nanoparticles via O atoms so as to alleviate the agglomeration of supported nanocatalysts. During the test, the mean particle size of catalysts increased from 2.5 nm and 2.6 nm to 5.3 nm and 4.8 nm for untreated and treated carbon support, respectively. Electrochemical surface area measurements also show that the oxidative treatment of carbon support enhanced the electrochemical stability of Pt/C catalysts. It is suggested that the chemical interaction between the metal particle and the oxygen-bearing functional group plays an important role in immobilizing catalyst nanoparticles. © 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.


Wang D.,Qingdao University | Ding F.,Jiangnan University
Digital Signal Processing: A Review Journal | Year: 2010

This paper uses an estimated noise transfer function to filter the input-output data and presents filtering based recursive least squares algorithms (F-RLS) for controlled autoregressive autoregressive moving average (CARARMA) systems. Through the data filtering, we obtain two identification models, one including the parameters of the system model, and the other including the parameters of the noise model. Thus, the recursive least squares method can be used to estimate the parameters of these two identification models, respectively, by replacing the unmeasurable variables in the information vectors with their estimates. The proposed F-RLS algorithm has a high computational efficiency because the dimensions of its covariance matrices become small and can generate more accurate parameter estimation compared with other existing algorithms. © 2010 Elsevier Inc. All rights reserved.


Ding F.,Jiangnan University
Digital Signal Processing: A Review Journal | Year: 2010

This paper considers connections between the cost functions of some parameter identification methods for system modelling, including the well known projection algorithm, stochastic gradient (SG) algorithm and recursive least squares (RLS) algorithm, and presents a modified SG algorithm by introducing the convergence index and a multi-innovation projection algorithm, a multi-innovation SG algorithm and a multi-innovation RLS algorithm by introducing the innovation length, aiming at improving the convergence rate of the SG and RLS algorithms. Furthermore, this paper derives an interval-varying multi-innovation SG and an interval-varying multi-innovation RLS algorithm in order to deal with missing data cases. © 2010 Elsevier Inc. All rights reserved.


Liu Y.,Jiangnan University | Yu L.,Jiangnan University | Ding F.,Jiangnan University
Circuits, Systems, and Signal Processing | Year: 2010

This paper derives the multi-innovation extended stochastic gradient algorithm for controlled autoregressive moving average models by expanding the scalar innovation to an innovation vector and analyzes its performance in detail. Four convergence theorems are given for the multi-innovation extended stochastic gradient algorithm to show that the parameter estimates converge to their true values under the weak persistent excitation condition. The simulation results show that the proposed algorithm can produce more accurate parameter estimates than the traditional extended stochastic gradient algorithm. © Springer Science+Business Media, LLC 2010.


Luo J.,Jiangnan University | Cong J.,Jiangnan University | Fang R.,Jiangnan University | Fei X.,Jiangnan University | Liu X.,Jiangnan University
Microchimica Acta | Year: 2014

A route is described for the preparation of a composite consisting of graphene oxide and a molecularly imprinted sol-gel polymer (GO/MIPs) through one-pot room temperature polymerization in aqueous solution. The material was obtained by mixing graphene oxide with the monomers (phenyltriethoxysilane and tetramethoxysilane) and the template paracetamol, followed by sol-gel copolymerization and extraction. The monomer and template concentrations and the incubation time were optimized. The composite was characterized by FTIR, TGA, XRD, Raman spectroscopy and SEM. It was then deposited as a thin film acting as a molecular recognition element on a glassy carbon electrode to obtain an electrochemical sensor for paracetamol. The electrode displayed an excellent recognition capacity toward paracetamol compared to its analogs. The peak current is linearly proportional to the concentration of paracetamol in the 0.1 μM to 80 μM range, and the detection limit is 20 nM (at an SNR of 3). Hence, this electrode possesses a wider response range and lower detection limit compared to most previously reported electrochemical sensors for paracetamol. It also exhibits excellent stability and has been successfully used to determine paracetamol in tablets and spiked human urine samples. [Figure not available: see fulltext.] © 2014 Springer-Verlag Wien.


Zhang S.-L.,Jiangnan University | Huang L.,Jiangnan University
Organic and Biomolecular Chemistry | Year: 2015

A Cu(i)-catalyzed cyanation reaction of aryl iodides with α-cyanoacetates is reported herein, which uses α-cyanoacetates as the nontoxic and easy-handling CN source through copper-mediated C-CN bond cleavage. This reaction enables access to aryl nitriles with an array of functional groups on the aromatic ring in good to excellent yields. © The Royal Society of Chemistry.


Zhang T.,Jiangnan University | Li Y.,Jiangnan University | Miao M.,Jiangnan University | Jiang B.,Jiangnan University
Food Chemistry | Year: 2011

An antioxidant peptide was purified using consecutive chromatographic methods from chickpea protein hydrolysates (CPH). This peptide was designated as Fra.7. It had a molecular weight of 717.37 Da, and its amino acid sequence was identified as Asn-Arg-Tyr-His-Glu by an ABI 4700 proteomics analyser. This antioxidant peptide was identified for the first time from food-derived protein hydrolysates. The molar ratio of the five amino acids in the sequence was 1:1:1:1:1. This antioxidant peptide efficiently quenched the free radical sources 1,1-diphenyl-2-pycryl-hydrazyl (DPPH), hydroxyl, and superoxide free radicals. The Cu2+ and Fe2+ chelating activities were 76.92% and 63.08% at the peptide concentration of 50 μg mL-1, respectively. Furthermore, the inhibition of the Fra.7 on lipid peroxidation was greater than that of α-tocopherol. The inhibition ratio of the linoleic acid autooxidation was 88.81% at the eighth day of analysis. © 2011 Elsevier Ltd. All rights reserved.


Wu Q.,Jiangnan University | Chen L.,Jiangnan University | Xu Y.,Jiangnan University
International Journal of Food Microbiology | Year: 2013

Yeasts are the most important group of microorganisms contributing to liquor quality in the solid-state fermentation process of Chinese Maotai-flavor liquor. There occurred a complex yeast community structure during this process, including stages of Daqu (the starter) making, stacking fermentation on the ground and liquor fermentation in the pits. In the Daqu making stage, few yeast strains accumulated. However, the stacking fermentation stage accumulated nine yeast species with different physio-biochemical characteristics. But only four species kept dominant until liquor fermentation, which were Zygosaccharomyces bailii, Saccharomyces cerevisiae, Pichia membranifaciens, and Schizosaccharomyces pombe, implying their important functions in liquor making. The four species tended to inhabit in different locations of the stack and pits during stacking and liquor fermentation, due to the condition heterogeneity of the solid-state fermentation, including the different fermentation temperature profiles and oxygen density in different locations. Moreover, yeast population was much larger in the upper layer than that in the middle and bottom layers in liquor fermentation, which was in accordance with the profile of reducing sugar consumption and ethanol production. This was a systematical investigation of yeast community structure dynamics in the Maotai-flavor liquor fermentation process. It would be of help to understand the fermentative mechanism in solid-state fermentation for Maotai-flavor liquor. © 2013 Elsevier B.V.


Wu Z.-S.,Jiangnan University | Zhang M.,Jiangnan University | Wang S.-J.,Washington State University
Journal of the Science of Food and Agriculture | Year: 2012

BACKGROUND: High-pressure (HP) inert gas processing causes inert gas and water molecules to form clathrate hydrates that restrict intracellular water activity and enzymatic reactions. This technique can be used to preserve fruits and vegetables. In this study, minimally processed (MP) pineapples were treated with HP (∼10 MPa) argon (Ar) and nitrogen (N) for 20 min. The effects of these treatments on respiration, browning and antioxidant potential of MP pineapples were investigated after cutting and during 20 days of storage at 4 °C. RESULTS: Lower respiration rate and ethylene production were found in HP Ar- and HP N-treated samples compared with control samples. HP Ar and HP N treatments effectively reduced browning and loss of total phenols and ascorbic acid and maintained antioxidant capacity of MP pineapples. They did not cause a significant decline in tissue firmness or increase in juice leakage. HP Ar treatments had greater effects than HP N treatments on reduction of respiration rate and ethylene production and maintenance of phenolic compounds and DPPH• and ABTS•+ radical-scavenging activities. CONCLUSION: Both HP Ar and HP N processing had beneficial effects on MP pineapples throughout 20 days of storage at 4 °C. © 2012 Society of Chemical Industry.


Yu X.-W.,Jiangnan University | Tan N.-J.,Jiangnan University | Xiao R.,Rutgers University | Xu Y.,Jiangnan University
PLoS ONE | Year: 2012

The key to enzyme function is the maintenance of an appropriate balance between molecular stability and structural flexibility. The lid domain which is very important for "interfacial activation" is the most flexible part in the lipase structure. In this work, rational design was applied to explore the relationship between lid rigidity and lipase activity by introducing a disulfide bond in the hinge region of the lid, in the hope of improving the thermostability of R. chinensis lipase through stabilization of the lid domain without interfering with its catalytic performance. A disulfide bridge between F95C and F214C was introduced into the lipase from R. chinensis in the hinge region of the lid according to the prediction of the "Disulfide by Design" algorithm. The disulfide variant showed substantially improved thermostability with an eleven-fold increase in the t1/2 value at 60°C and a 7°C increase of Tm compared with the parent enzyme, probably contributed by the stabilization of the geometric structure of the lid region. The additional disulfide bond did not interfere with the catalytic rate (kcat) and the catalytic efficiency towards the short-chain fatty acid substrate, however, the catalytic efficiency of the disulfide variant towards pNPP decreased by 1.5-fold probably due to the block of the hydrophobic substrate channel by the disulfide bond. Furthermore, in the synthesis of fatty acid methyl esters, the maximum conversion rate by RCLCYS reached 95% which was 9% higher than that by RCL. This is the first report on improving the thermostability of the lipase from R. chinensis by introduction of a disulfide bond in the lid hinge region without compromising the catalytic rate. © 2012 Yu et al.


The unitary correlation operator method (UCOM) and the similarity renormalization group theory (SRG) are compared and discussed in the framework of the no-core Monte Carlo shell model (MCSM) calculations for 3H and 4He. The treatment of spurious center-of-mass motion by Lawson's prescription is performed in the MCSM calculations. These results with both transformed interactions show good suppression of spurious center-of-mass motion with proper Lawson's prescription parameter βc.m. values. The UCOM potentials obtain faster convergence of total energy for the ground state than that of SRG potentials in the MCSM calculations, which differs from the cases in the no-core shell model calculations (NCSM). These differences are discussed and analyzed in terms of the truncation scheme in the MCSM and NCSM, as well as the properties of the potentials of SRG and UCOM. © 2015 Chinese Physical Society and the Institute of High Energy Physics of the Chinese Academy of Sciences and the Institute of Modern Physics of the Chinese Academy of Sciences and IOP Publishing Ltd.


Liu L.,Jiangnan University
Chinese Physics C | Year: 2015

The low-lying spectra of 7Li and 9Li are investigated within an ab initio Monte Carlo Shell Model (MCSM) employing a realistic potential obtained via the Unitary Correlation Operator Method (UCOM). The MCSM calculations in a 4-major-shells model space for the binding energy and mass quadrupole moment of 7,9Li show good convergence when the MCSM dimension reaches 20. The excitation energy of the Jπ = 1/2- state for 7Li and the magnetic moments for 7,9Li ground states in the MCSM with a treatment of spurious center-of-mass motion are close to the experimental data. Correct level ordering of Jπ = 3/2- and 1/2- states for 7,9Li can be reproduced due to the inclusion of three-body correlations in the MCSM+UCOM. However, the excitation energy of Jπ = 1/2- state for 9Li is not reproduced in the MCSM mainly due to the lack of larger model space. ©2015 Chinese Physical Society and the Institute of High Energy Physics of the Chinese Academy of Sciences and the Institute of Modern Physics of the Chinese Academy of Sciences and IOP Publishing Ltd.


Sun S.-Y.,Jiangnan University | Fang Y.,Jiangnan University | Xia Y.-M.,Jiangnan University
Food Control | Year: 2012

The acrylamide content in microwaved food has been highly concerned because of food safety; meanwhile, the detection of acrylamide content in starchy food is difficult due to the stickiness. In this experiment, a facile MS-free method was demonstrated to determine acrylamide content in microwaved and conventional heated popcorn and rice, using activated carbon packed extraction column coupling with simple GC analysis. The detection limit of acrylamide is 10 ng/mL. The recovery ratio of acrylamide in sample was 100.60% with a RSD of 1.2%-4.6%. The detection RSD of the acrylamide contents in microwaved caramel sweet popcorn and cooked rice were 1.46% (n = 10) and 1.20% (n = 3), respectively. © 2012 Elsevier Ltd.


Yang Y.,Jiangnan University | Yang Y.,Purdue University | Campanella O.H.,Purdue University | Hamaker B.R.,Purdue University | And 2 more authors.
Food Hydrocolloids | Year: 2013

Chain interactions of sodium alginate during its gelation were investigated by a new gelation method which was based on a Ca 2+-concentrating gelling process (CCGP) produced by water evaporation of an alginate solution containing CaCl 2. For two commercially available sodium alginate samples (low viscosity (LA) and medium viscosity (MA)) having different molecular weight distributions but the same G/M blocks, the critical Ca 2+ concentrations for their gelation were found to be 4.6 (for LA) and 4.5 (for MA) μmol/mL after evaporating water from 1% of alginate solutions containing 4 μmol/mL of CaCl 2. The CCGP gelation method for alginate under the above conditions were confirmed by rheological measurements and the observed highly ordered and uniform mesh structure of the CCGP-formed alginate gels shown in cryo-SEM images. Combinations of LA and MA at different ratios (0:4, 1:3, 2:2, 3:1, 4:0 on weight basis) were studied using the CCGP gelation method to further the understanding of the alginate chain interactions during gelation. Different LA/MA mixtures exhibited different rheological properties in either non-gelled or gelled systems, indicating that the molecular weight distributions of the sodium alginates influence the alginate chain interactions mediated by Ca 2+. Thus, an appropriate combination of LA and MA is required for a strong alginate interchain interaction during CCGP, and alginate products with desirable characteristics can be produced by manipulating the mixing ratios of sodium alginates having different molecular weight distributions even at the same total composition and distribution of G/M blocks. © 2012 Elsevier Ltd.


Wang X.,Jiangnan University | Zhu K.-X.,Jiangnan University | Zhou H.-M.,Jiangnan University
International Journal of Molecular Sciences | Year: 2011

In order to improve its stability and catalytic rate in flour, the immobilization of glucose oxidase (GOX) was investigated in this work. The enzyme was encapsulated in calcium alginate-chitosan microspheres (CACM) using an emulsification-internal gelation-GOX adsorption-chitosan coating method. The interaction between alginate and chitosan was confirmed by infrared spectroscopy (IR). The resultant CACM in wet state, whose morphology was investigated by scanning electron microscopy (SEM), was spherical with a mean diameter of about 26 μm. The GOX load, encapsulation efficiency and activity of the CACM-GOX were influenced by concentration of chitosan, encapsulation time and encapsulation pH. The highest total enzymatic activity and encapsulation efficiency was achieved when the pH of the adsorption medium was near the isoelectric point (pI) of GOX, approximately pH 4.0. In addition, the molecular weight of chitosan also evidently influenced the encapsulation efficiency. Storage stabilities of GOX samples were investigated continuously over two months and the retained activity of CACM-GOX was 70.4%, markedly higher than the 7.5% of free enzyme. The results reveal the great potential of CACM-GOX as a flour improver. © 2011 by the authors.


Lu X.-Y.,Jiangnan University
International Journal of Control and Automation | Year: 2015

Intelligent tower crane has large structure deadweight. The service life of the whole machine is dependent on the life of the structure. As a result, the metal structure should be designed by using correct and reasonable methods to obtain excellent fatigue properties. And it can guide the design, manufacturing, use and maintenance process for crane. And it has great significance to prevent the fatigue fracture accident. The theoretical mechanics and material mechanics theory is a semi-theoretical semi-empirical traditional design method, but it is not the suitable design method. Now, the finite element method, fracture mechanics, the boundary element method, the optimization design method, reliability design and fatigue design methods are widely used in the structure design of crane. In this paper, the intelligent tower crane structure is analyzed by finite element modeling. According to the actual situation, crane structure is studied by finite element statics analysis. Then mechanical property of this kind of structure is analyzed in detail. And the weak links of the structure are found out. The residual life can be estimated finally. As a result, designers can comprehensively understand the fatigue life distribution of different parts in the crane. Therefore, they can provide intuitive and comprehensive basis for comparing the advantages and disadvantages of different design schemes and fatigue performances for design improvement. © 2015 SERSC.


Sun J.,Jiangnan University | Chen W.,Jiangnan University | Fang W.,Jiangnan University | Wun X.,Jiangnan University | Xu W.,Jiangnan University
Engineering Applications of Artificial Intelligence | Year: 2012

Microarray technology has been widely applied in study of measuring gene expression levels for thousands of genes simultaneously. In this technology, gene cluster analysis is useful for discovering the function of gene because co-expressed genes are likely to share the same biological function. Many clustering algorithms have been used in the field of gene clustering. This paper proposes a new scheme for clustering gene expression datasets based on a modified version of Quantum-behaved Particle Swarm Optimization (QPSO) algorithm, known as the Multi-Elitist QPSO (MEQPSO) model. The proposed clustering method also employs a one-step K-means operator to effectively accelerate the convergence speed of the algorithm. The MEQPSO algorithm is tested and compared with some other recently proposed PSO and QPSO variants on a suite of benchmark functions. Based on the computer simulations, some empirical guidelines have been provided for selecting the suitable parameters of MEQPSO clustering. The performance of MEQPSO clustering algorithm has been extensively compared with several optimization-based algorithms and classical clustering algorithms over several artificial and real gene expression datasets. Our results indicate that MEQPSO clustering algorithm is a promising technique and can be widely used for gene clustering. © 2011 Elsevier Ltd. All rights reserved.


Ding F.,Jiangnan University
Chinese Control Conference, CCC | Year: 2013

In this paper, the projection identification algorithm is extended and the generalized projection algorithm is presented. The analysis indicates that the generalized projection algorithm can track the time-varying parameters and has the same properties as the forgetting factor least squares algorithms but has less computational effort. The way of choosing the data window length is stated so as to obtain the minimum upper bound of the parameter estimation errors. © 2013 TCCT, CAA.


Zhao S.,Jiangnan University | Liu F.,Jiangnan University
Journal of the Franklin Institute | Year: 2012

In this paper, an adaptive risk-sensitive multiple-model filtering method which relaxes the restrictive assumption that risk-sensitive parameter is chosen as a prior is proposed for a class of discrete-time Markov jump linear systems (MJLSs) with uncertain parameters. Some analysis is presented to illustrate the essential effect of the risk sensitivity added into the filtering process and show the intrinsic reasons for the improvement of robustness. Then, a quite useful principle is developed to obtain the risk-sensitive parameter using the measurements in an online fashion. To avoid overregulation under mismatched modes and mitigate the problem of smearing the feature of each model, a minimization mechanism is resorted to. Computer simulations are presented to reveal the effectiveness of our method. © 2012 The Franklin Institute. All rights reserved.


Xu J.,Jiangnan University | Zhang H.,Jiangnan University | Guo X.,Jiangnan University | Qian H.,Jiangnan University
Journal of the Science of Food and Agriculture | Year: 2012

BACKGROUND: In recent years, germinated brown rice as a functional food has received great attention with its improved sensory and nutritional properties. Particularly of interest are the high levels of γ-amino butyric acid (GABA) which can be obtained during germination. However, more studies are needed to fully understand the effect of germination on the physicochemical properties of brown rice. RESULT: Germination altered the chemical composition of brown rice, resulting in an increase in reducing sugar and ash content, and a reduction in amylose. Solubility, paste viscosity, transition temperatures (T o, T p and T c) and percentage of retrogradation (%Retrogradation) were decreased, while swelling power and turbidity were significantly increased. Scanning electron micrographs indicated that starch granules from germinated brown rice became smaller and less homogeneous. Moreover, germination shortened the chain length of amylopectin and amylose molecules. CONCLUSION: This investigation provides information on changes in the characteristics of rice flour and rice starch during germination, leading to a better understanding on the chemistry of brown rice germination. © 2011 Society of Chemical Industry.


Zhang H.,Jiangnan University | Zhang H.,U.S. Department of Agriculture | Yokoyama W.H.,U.S. Department of Agriculture
Journal of the Science of Food and Agriculture | Year: 2012

Background: Rice bran, containing about 100-150 g kg -1 protein, is a by-product of rice milling that has only become an available ingredient in recent years owing to the centralisation of rice milling. Rice bran, but not its protein fraction or hydrolysates, has been shown to have a hypocholesterolaemic effect. Peptides from soy, milk and other foods have been proposed to have hypocholesterolaemic effects based on their ability to lower cholesterol solubility in bile acid/phosphotidyl choline micelles. Results: Rice bran protein hydrolysates (RBPHs) were prepared and investigated for their potential to lower cholesterol concentration in micelles. The RBPHs were produced by digestion using four different peptidases, alcalase 2.4L ®, neutrase 0.8L ®, papaya latex papain and porcine pancreas trypsin, and then fractionated by hydrophobicity using styrene/divinylbeneze resins. Alcalase 2.4L ® produced the highest degree of hydrolysis, and the resulting hydrolysates had the highest micellar cholesterol inhibition ability in an in vitro hypocholesterolaemic test. The adsorption dynamics of four different macroporous resins, DA201-C, Sepabeads SP207 and SP825 and Diaion HP20, were determined using the Langmuir isotherm model. DA201-C had the highest adsorption capacity with an equilibrium concentration of 220 mg g -1. The hydrolysates eluted with 25, 50, 75 and 95% (v/v) ethanol lowered the micellar cholesterol concentration by 11.88, 14.76, 19.37 and 7.56% respectively. Conclusion: A hydrophobic fraction of RBPH had the highest inhibitory activity on micellar cholesterol, which suggests that it may have hypocholesterolaemic properties. © 2011 Society of Chemical Industry.


Wu Z.S.,Jiangnan University | Zhang M.,Jiangnan University | Wang S.,Washington State University
Food Control | Year: 2012

High pressure (HP) argon (Ar) processing that makes argon and water molecules form clathrate hydrates to restrict intracellular water activity and enzymatic reactions. This can be used in the preservation of fruits and vegetables. Effects of HP (150 MPa) Ar treatments on preserving fresh-cut apples were investigated at 4 °C for two weeks. Respiration rate and ethylene production of fresh-cut apples treated by the HP (150 MPa) Ar for 10 min were lower apparently in comparison with those untreated and treated by flushing with Ar and the HP air for 10 min. The HP Ar treatment delayed browning and microbial growth of fresh-cut apples at cold storage, because the populations of both mesophiles and psychrotrophs did not exceed 6.0 log cfu/g, and those for molds and yeasts were less than 3.0 log cfu/g after two weeks storage. HP Ar treatment did not affect the content of titrated acidity and soluble solids, and significantly reduced the total phenolics decrease in fresh-cut apples (p < 0.05). Dipping into chemical solution, such as 0.5% ascorbic acid (AA), 0.5% citric acid (CA) and 0.5% calcium chloride (CC) for 5 min could reduce the changes in the color and firmness of apple wedges during HP operation, and the combined HP Ar with dipping treatment remained good sensory attributes of fresh-cut apples for 12 days at 4 °C. These results indicated the HP Ar treatment could be an effective method for improving the quality of fresh-cut apples at cold storage conditions. © 2011 Elsevier Ltd.


Wu C.,Jiangnan University | Zhang J.,Jiangnan University | Chen W.,Jiangnan University | Wang M.,Jiangnan University | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Lactobacillus casei has traditionally been recognized as a probiotic and frequently used as an adjunct culture in fermented dairy products, where acid stress is an environmental condition commonly encountered. In the present study, we carried out a comparative physiological and proteomic study to investigate lactic-acid-induced alterations in Lactobacillus casei Zhang (WT) and its acid-resistant mutant. Analysis of the physiological data showed that the mutant exhibited 33.8% higher glucose phosphoenolpyruvate:sugar phosphotransferase system activity and lower glycolytic pH compared with the WT under acidic conditions. In addition, significant differences were detected in both cells during acid stress between intracellular physiological state, including intracellular pH, H +-ATPase activity, and intracellular ATP pool. Comparison of the proteomic data based on 2D-DIGE and i-TRAQ indicated that acid stress invoked a global change in both strains. The mutant protected the cells against acid damage by regulating the expression of key proteins involved in cellular metabolism, DNA replication, RNA synthesis, translation, and some chaperones. Proteome results were validated by Lactobacillus casei displaying higher intracellular aspartate and arginine levels, and the survival at pH 3.3 was improved 1.36- and 2.10-fold by the addition of 50-mM aspartate and arginine, respectively. To our knowledge, this is the first demonstration that aspartate may be involved in acid tolerance in Lactobacillus casei. Results presented here may help us understand acid resistance mechanisms and help formulate new strategies to enhance the industrial applications of this species. © 2011 Springer-Verlag.


Gong J.-S.,Jiangnan University | Lu Z.-M.,Jiangnan University | Li H.,Jiangnan University | Zhou Z.-M.,Jiangnan University | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2013

Nitrilase is one of the most important members in the nitrilase superfamily and it is widely used for bioproduction of commodity chemicals and pharmaceutical intermediates as well as bioremediation of nitrile-contaminated wastes. However, its application was hindered by several limitations. Searching for new nitrilases and improving their application performances are the driving force for researchers. Genetic data resources in various databases are quite rich in post-genomic era. Besides, more than 99 % of microbes in the environment are unculturable. Metagenomic technology and genome mining are thus becoming burgeoning areas and provide unprecedented opportunities for searching more useful novel nitrilases due to the abundance of already existing but unexplored gene resources, namely uncharacterized genome information in the database and unculturable microbes in the natural environment. These techniques seem to be innovative and highly efficient. This study reviews the current status and future directions of metagenomics and genome mining in nitrilase exploration. Moreover, it discussed their utilization in coping with the challenges for nitrilase application. In the next several years, with the rapid development of nitrile biocatalysis, these two techniques would be bound to attract increasing attentions and even become a dominant trend for finding more novel nitrilases. Also, this review would provide guidance for exploitation of other commercially important enzymes. © 2013 Springer-Verlag Berlin Heidelberg.


Mu W.,Jiangnan University | Chen Q.,Jiangnan University | Wang X.,Jiangnan University | Zhang T.,Jiangnan University | Jiang B.,Jiangnan University
Applied Microbiology and Biotechnology | Year: 2013

Lactosucrose (O-β-d-galactopyranosyl-(1,4)-O-α-d-glucopyranosyl- (1,2)-β-d-fructofuranoside) is a trisaccharide formed from lactose and sucrose by enzymatic transglycosylation. This rare trisaccharide is a kind of indigestible carbohydrate, has good prebiotic effect, and promotes intestinal mineral absorption. It has been used as a functional ingredient in a range of food products which are approved as foods for specified health uses in Japan. Using lactose and sucrose as substrates, lactosucrose can be produced through transfructosylation by β-fructofuranosidase from Arthrobacter sp. K-1 or a range of levansucrases, or through transgalactosylation by β-galactosidase from Bacillus circulans. This article presented a review of recent studies on the physiological functions of lactosucrose and the biological production from lactose and sucrose by different enzymes. © 2013 Springer-Verlag Berlin Heidelberg.


Shi F.,Jiangnan University | Huan X.,Jiangnan University | Wang X.,Jiangnan University | Ning J.,Jinghai Amino Acid Liability Ltd Company
Enzyme and Microbial Technology | Year: 2012

NADPH is the key cofactor in l-isoleucine (Ile) biosynthetic pathway. To increase the Ile biosynthesis in Corynebacterium glutamicum ssp. lactofermentum JHI3-156, NADPH supply needs to be enhanced. Here NAD kinase, the key enzyme for the de novo biosynthesis of NADP + and NADPH, were cloned and expressed in JHI3-156, and their influences on Ile production were analysed. Meanwhile, enzyme properties of NAD kinase from JHI3-156 (CljPpnK) were compared with that from C. glutamicum ssp. lactofermentum ATCC 13869 (ClPpnK). Four variations existed between CljPpnK and ClPpnK. Both PpnKs were poly(P)/ATP-dependent NAD kinases that used ATP as the preferred phosphoryl donor and NAD + as the preferred acceptor. CljPpnK exhibited a higher activity and stability than ClPpnK and less sensitivity towards the effectors NADPH, NADP +, and NADH, partly due to the variations between them. The S57P variation decreased their activity. Expression of CljppnK and ClppnK in JHI3-156 increased the ATP-NAD + kinase activity by 69- and 47-fold, respectively, the intracellular NADP + concentration by 36% and 101%, respectively, the NADPH concentration by 95% and 42%, respectively, and Ile production by 37% and 24%, respectively. These results suggest that overexpressing NAD kinase is a useful metabolic engineering strategy to improve NADPH supply and isoleucine biosynthesis. © 2012 Elsevier Inc.


Liu S.,Wuhan University | Liu S.,Jiangnan University | Zhou J.,Wuhan University | Zhang L.,Wuhan University
Journal of Physical Chemistry C | Year: 2011

Plate-like Fe2O3 nanoparticles were prepared in microporous regenerated cellulose films and then completely removed from the cellulose matrix by calcination. Crystallite size and properties variations of the nanosized Fe2O3 during γ- to α-phase transformation were investigated. The γ-Fe2O3 nanoparticles with an initial particle size of 24 nm increased from 26 to 83 nm with an increase of the calcination temperature from 350 to 800 C, and the phase transformation temperature was about 500 C. The nanoparticles made at 350 and 450 C exhibited superparamagnetic properties with the corresponding blocking temperature (TB) of about 83 and 80 K. The nanoparticles made at 500, 600, and 700 C not only exhibited Curie transitions with TB's of about 77, 54, and 15 K but also had Morin transitions from a pure antiferromagnet to a ferromagnet occurring at about 238, 240, and 246 K, respectively. However, the nanoparticles made at 800 C only gave a clear Morin transition from a pure antiferromagnet to a ferromagnet at about 250 K. Furthermore, the nanoparticles also displayed different electrochemical properties. © 2011 American Chemical Society.


Yuan C.,Anyang Institute of Technology | Jin Z.,Jiangnan University | Xu X.,Jiangnan University
Carbohydrate Polymers | Year: 2012

The structure and complex mode of the inclusion complex of astaxanthin with hydroxypropyl-β-cyclodextrin (HPCD) were investigated by UV, FTIR, 1H NMR and molecular modeling test. UV, FTIR and 1H NMR results indicated that the hexatomic ring of the astaxanthin molecules were partly included into the HPCD cavities. The implementation of molecular modeling test confirmed that the complexation could reduce the energy of the system and the complex of 2:1 host-guest stoichiometry had the lowest ΔE value, -30.57 kcal/mol, two hexatomic ring ends of one astaxanthin molecule inserted into two HPCD cavities respectively, and that should be the most predominant configuration. © 2012 Elsevier Ltd. All rights reserved.


Liu S.,Jiangnan University | Yan Q.,Jiangnan University | Tao D.,Jiangnan University | Yu T.,Jiangnan University | Liu X.,Jiangnan University
Carbohydrate Polymers | Year: 2012

Nanostructured cellulose nanofibrils can form ductile or tough networks that are suitable templates for the creation of materials with functional properties. In this work, a facile method has been developed for the preparation of magnetic hybrid cellulose aerogels. The preparation processes followed by two steps, firstly, preparation of cellulose hydrogel films from LiOH/urea solvent, then CoFe 2O 4 nanoparticles were synthesized in the porous structured cellulose scaffolds. After being freeze-dried, CoFe 2O 4/cellulose magnetic aerogels were obtained. The porosity of the composite aerogels ranged from 78 to 52% with pore size distribution in a few tens of nanometers. The internal specific surface areas were around 300-320 m 2/g, and the densities were in the range of 0.25-0.39 g/cm 3. The hybrid aerogels showed improved mechanical strength, superparamagnetic properties. Unlike solvent-swollen gels and ferrogels, the magnetic composite aerogels were lightweight, flexibility, high porosity and with large specific surface area and could be expected to be used in many fields. © 2012 Elsevier Ltd. All rights reserved.


Zhu B.-F.,Jiangnan University | Xu Y.,Jiangnan University
Journal of Industrial Microbiology and Biotechnology | Year: 2010

The effects of initial culture pH ranging from 5.0 to 7.5 on biomass content, precursor 3-hydroxy-2-butanone (HB) accumulation, and 2,3,5,6-tetramethylpyrazine (TTMP) formation by Bacillus subtilis CCTCC M 208157 were investigated in shake flask fermentation. Weak acidic conditions were found to favor cell growth and precursor HB accumulation, while TTMP could be synthesized more efficiently in conditions with initial pH towards neutrality. Batch bioprocess of TTMP fermentation by Bacillus subtilis CCTCC M 208157 at various controlled pH values ranging from 5.5 to 7.0 was then examined in 7.5-l fermentor. The results suggested that optimum pH for cell growth and precursor HB accumulation was 5.5 with maximum cell growth rate (Q x) and precursor HB accumulation rate (Q HB) of 0.833 g l-1 h-1 and 1.118 g l-1 h-1, respectively, while optimum pH for TTMP formation was 7.0 with maximum TTMP formation rate (Q TTMP) of 0.095 g l-1 h-1. A pH-shifted strategy was accordingly developed to improve TTMP production in bioreactor fermentation by shifting the culture pH from 5.5 to 7.0 after 48 h of cultivation. By applying the strategy, final TTMP concentration of 7.43 g l-1 was obtained, being 22.2% greater than that of constant-pH fermentation. © 2010 Society for Industrial Microbiology.


Mu W.,Jiangnan University | Li Q.,Jiangnan University | Fan C.,Jiangnan University | Zhou C.,Jiangnan University | Jiang B.,Jiangnan University
Applied Microbiology and Biotechnology | Year: 2013

Epilactose (4-O-β-d-galactopyranosyl-d-mannose), an epimer of lactose, is a rare disaccharide existing extremely small quantities in heat-treated milk, in which epilactose is produced by non-enzymatic catalysis from lactose. This disaccharide is a kind of non-digestible carbohydrate, has a good prebiotic effect, and promotes intestinal mineral absorption. This article presents a review of recent studies on epilactose formation in food system, qualitative and quantitative analysis, and its physiological functions. In addition, the biochemical properties and kinetic parameters of the epilactose-producing enzyme, cellobiose 2-epimerase, are compared, and the biotechnological production of epilactose from lactose is reviewed. © 2013 Springer-Verlag Berlin Heidelberg.


Yan W.-X.,Jiangnan University | Ji Z.-C.,Jiangnan University
Dianji yu Kongzhi Xuebao/Electric Machines and Control | Year: 2010

Aim to solve the inefficiency of controlling the nonlinear of three-phase Shunt Active Power Filter (SAPT) by the traditional Proportion Integration (PI) controller, Takagi-Sugeno (T-S) type fuzzy logic method was put forwards. Based on the mathematics model analysis of the three-phase SAPT, T-S fuzzy logic model was established and fuzzy controller was designed. With analysis and demonstration the stability of the proposed T-S model, parallel distributed compensation was employed to design with just four rules fuzzy controllers for the T-S fuzzy models, and the common Lyapunov function and the stable feedback gains were solved by linear matrix inequality via Matlab. Computer simulations tested on a three-phase power system indicate the correctness, validity and significant performance with reactive power and harmonic compensation by the T-S fuzzy controller.


Peng Q.,Jiangnan University
Applied Mechanics and Materials | Year: 2013

Spatial indexing cam is widely used in some automation machineries, and usually can be classified into the following three types: (1) cylindrical indexing cam; (2) parallel indexing cam; (3)globular indexing cam. These cams have their own use range, and also are different in index number and application accuracy. In this paper, cylindrical indexing cam is mainly analyzed. © (2013) Trans Tech Publications, Switzerland.


Gao Q.-H.,Northwest University, China | Wu C.-S.,Jiangnan University | Wang M.,Northwest University, China
Journal of Agricultural and Food Chemistry | Year: 2013

The nutritional jujube (Ziziphus jujube Mill.) fruit belonging to the Rhamnaceous family grows mostly in Europe, southern and eastern Asia, and Australia, especially the inland region of northern China. Jujube has a long history of usage as a fruit and remedy. The main biologically active components are vitamin C, phenolics, flavonoids, triterpenic acids, and polysaccharides. Recent phytochemical studies of jujube fruits have shed some light on their biological effects, such as the anticancer, anti-inflammatory, antiobesity, immunostimulating, antioxidant, hepatoprotective, and gastrointestinal protective activities and inhibition of foam cell formation in macrophages. A stronger focus on clinical studies and phytochemical definition of jujube fruits will be essential for future research efforts. This review may be useful for predicting other medicinal uses and potential drug or food interactions and may be beneficial for people living where the jujube fruits are prevalent and health care resources are scarce. © 2013 American Chemical Society.


Liu G.,Jiangnan University
Applied Mechanics and Materials | Year: 2010

As we all know everyone has IQ (Intelligence Quotient) and EQ (Emotion Quotient), but for designers DQ (Design Quotient) is much more important than the IQ and EQ. In this paper first the DQ situation of students major in Product Design in China is analyzed, and then the qualities required for studying design are explained in depth. There are two design qualities, one is Innate Quality (character factors), and the other is Acquired Quality (study factors). Finally the approaches of cultivating and improving design students' DQ are discussed in detail from two levels - Concrete level and Metaphysical level. © (2010) Trans Tech Publications.


Liu L.,Jiangnan University
Information Sciences | Year: 2013

The aim of this paper is to investigate the existence of Bosbach states and Riečan states on MTL-algebras. We prove that an MTL-algebra L has Bosbach states if and only if L has an MV-filter. We also establish that L has a state-morphism if and only if L has a maximal MV-filter. Furthermore, we obtain the necessary and sufficient condition for an MTL-chain having Riečan states. © 2012 Elsevier Inc. All rights reserved.


Jia L.,Jiangnan University | Wang X.,Jiangnan University | Zhang M.,Jiangnan University | Han Y.,Jiangnan University
Nonlinear Dynamics | Year: 2012

The weakly nonholonomic system is a nonholonomic system whose constraint equations contain a small parameter. The special Mei symmetry and approximate conserved quantity of Appell equations for a weakly nonholonomic system are studied. Appell equations for a weakly nonholonomic system are established and the definition and the criterion of the special Mei symmetry of the system are given. The structure equation of the special Mei symmetry for a weakly nonholonomic system and approximate conserved quantity deduced from the special Mei symmetry of the system are obtained. Finally, special approximate conserved quantity issues of Appell equations for a two freedom degrees weakly nonholonomic system are investigated using the results of this paper. © Springer Science+Business Media B.V. 2012.


Li L.,Jiangnan University | Fang Z.,Jiangnan University | Yang Y.,Jiangnan University
Nonlinear Analysis: Real World Applications | Year: 2012

In this paper, a shunting inhibitory cellular neural network with continuously distributed delays of neutral type is considered. We establish some new results about the existence and exponential stability of the almost periodic solution for the shunting inhibitory cellular neural network. © 2011 Elsevier Ltd. All rights reserved.


Feng W.,Jiangnan University
2010 IEEE 11th International Conference on Computer-Aided Industrial Design and Conceptual Design, CAID and CD'2010 | Year: 2010

In this paper, the background of digital age is briefly analysed, and then it illustrates the development of media materials in public art on terms of both traditional and digital age. Afterwards, it furture researches the application of media materials in public art through the case study of materials related to imaging, photoelectric, inductive and composite in the context of the digital age. © 2010 IEEE.


Qian S.,Jiangnan University
Industrial Lubrication and Tribology | Year: 2015

Purpose - This paper aims to present the probable factors resulting in the lubrication failure in detail, based on the experimental study on the tribological property of the low-viscosity lubricant subjected to the different slide/roll ratios and loads under micro confined space. Design/methodology/approach - The interference images and the traction coefficients of the spindle oil with low viscosity were recorded using a ball-on-disc test rig. Moreover, the corresponding flash temperatures were obtained via an analytical method. Findings - More scratches can be observed in the interference images with higher slide/roll ratios. The applied load plays a significant role in the variation of the traction coefficient under different slide/roll ratio, and higher load resulted in lower traction coefficient. The flash temperature generated in the point contact zone non-linearly increases with increasing slide/roll ratio. Originality/value - The flash temperature is not a crucial factor which results in these scratches in the interference images. Moreover, it is probable that the micro confined space is in boundary lubrication at higher shear rates. © Emerald Group Publishing Limited.


Zhang W.,Jiangnan University | Zhang J.,Jiangnan University | Jiang Q.,Jiangnan University | Xia W.,Jiangnan University
Carbohydrate Polymers | Year: 2012

Two different molecular weights of chitosan were pulverized to nanopowders by ultrafine milling. The nanopowders were characterized by viscometry small angle X-ray scattering (SAXS), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA), FT-IR spectroscopy and UV-vis spectroscopy. Our results showed that ultrafine milling effectively reduced the particle size of chitosan to a nanoscale. The viscosity average molecular weight (Mv) of chitosan was decreased by the milling treatment. The crystalline structure of chitosan was destroyed by the milling since the nanopowder exhibited an amorphous XRD pattern. In addition, thermal stability of the low molecular weight chitosan was decreased after the milling treatment. FT-IR and UV-vis spectra showed that the milling process did not cause significant changes in the chemical structure of chitosan. © 2011 Elsevier Ltd. All Rights Reserved.


Wang Z.,Jiangnan University | Li Z.,Jiangnan University | Gu Z.,Jiangnan University | Hong Y.,Jiangnan University | Cheng L.,Jiangnan University
Carbohydrate Polymers | Year: 2012

A new biodegradable, renewable, environmentally friendly starch-based wood adhesive that can be used at room temperature was synthesized by the graft polymerization of vinyl acetate monomer onto waxy corn starch. Compared with the blend of commercial PVAc/gelatinized starch, the shear strength of grafted starch adhesive increased by 59.4% in dry state and 321% in wet state, while its water resistance increased by 61.1%. The improved performance of the grafted starch-based wood adhesive was supported by its strengthened compatibility with polyvinyl acetate and starch, based on the results of Fourier-transform infrared, 1H nuclear magnetic resonance, thermogravimetry, and scanning electron microscopy analyses. The most economic monomer feeding ratio was also evaluated using bond characteristics and grafting parameters. A starch/monomer ratio of 1:1.2 (w/w) was considered as the best ratio in terms of economy. © 2011 Elsevier Ltd. All rights reserved.


Yang J.S.,Jiangnan University | Yang J.S.,Yangzhou University | Jiang B.,Yangzhou University | He W.,Yangzhou University | Xia Y.M.,Jiangnan University
Carbohydrate Polymers | Year: 2012

Dodecanol was covalently coupled to sodium alginate (NaAlg) via ester functions using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC-HCl) as a coupling reagent to provide an amphiphilic dodecanol alginate (DA) for subsequent use in oil-in-water (O/W) emulsion application. The structure of DA was confirmed by FT-IR spectrometry. The stability of the emulsions prepared with different concentrations (0.3-1.2 wt%) of DA or 1.0 wt% NaAlg was evaluated by measuring droplet size, microstructure, viscosity and creaming. The results showed that the emulsions containing 1.0 wt% NaAlg, 0.3 and 0.5 wt% DA were unstable and the emulsions containing 0.8-1.2 wt% DA presented better stability during storage. © 2011 Elsevier Ltd. All rights reserved.


Xiao Q.,Jiangnan University | Lim L.-T.,University of Guelph | Tong Q.,Jiangnan University
Carbohydrate Polymers | Year: 2012

Pullulan-sodium alginate blend films were prepared and characterized as a function of water activity (aw). At low aw, the incorporation of alginate into pullulan film increased the tensile strength and elastic modulus, but decreased the elongation at break of the composite films; the opposite trends were observed at elevated aw. Above 0.43 a w, water exerted a typical plasticization effect upon the biopolymer blends. As aw increased from 0.23 to 0.43, an anti-plasticization effect was observed as tensile strength and elastic modulus increased. The glass transition temperature of all samples decreased substantially as aw increased from 0.23 to 0.84 due to the plasticization effect of water. Within this a w range, one transition temperature was observed for all film specimens. The stretching vibration band of O-H was investigated using attenuated total reflection Fourier transform infrared spectroscopy to identify the various species of water interacting with the polysaccharide films. © 2011 Elsevier Ltd. All Rights Reserved.


Chen J.,Jiangnan University | Zhang T.,Jiangnan University | Jiang B.,Jiangnan University | Mu W.,Jiangnan University | Miao M.,Jiangnan University
Carbohydrate Polymers | Year: 2012

Ginkgo biloba exocarp polysaccharide (GBEP) was obtained by hot water extraction, the crude polysaccharide was deproteinized by Sevag method and fractionized by a DEAE Sepharose fast flow anion-exchange column. Five fragments were obtained, including neutral polysaccharide (GBEP-N) and four acidic polysaccharides (GBEP-A1, GBEP-A2, GBEP-A3 and GBEP-A4). GBEP-N and GBEP-A3 were further purified by Superdex 200 gel column chromatography. The resulted two fractions GBEP-NN, and GBEP-AA were characterized by FT-IR, and HPGFC (high pressure gel filtration chromatography). Monosaccharide composition was determined by RP-HPLC method of precolumn derivatization with 1-phenyl-3-5-pyrazolone. GBEP-NN was mainly composed of rhamnose, arabinose, mannose, glucose and galactose, while GBEP-AA was mainly made up of mannose, rhamnose, glucuronic acid, galacturonic acid, galactosamine, glucose, galactose, xylose, arabinose, and fucose. The crude GBEP exhibited certain antioxidant activity. At the concentration of 5 mg/mL, the hydroxyl radical scavenging effect of GBEP was 90.52%, greater than 77.37% for the positive control ascorbic acid. © 2011 Elsevier Ltd. All Rights Reserved.


Fan L.,Jiangnan University | Li J.,Jiangnan University | Deng K.,Jiangnan University | Ai L.,Bright Dairy and Food Co.
Carbohydrate Polymers | Year: 2012

Three polysaccharides (GLP-H, GLP-V and GLP-F) were obtained from Ganoderma lucidum by hot air drying, vacuum drying and vacuum freeze drying, respectively. Their chemical compositions were determined, and antioxidant activities were investigated on the basis of superoxide radical, hydroxyl radical, reducing power, DPPH free radical and Ferric-reducing antioxidant power (FRAP) assay. The results showed that three polysaccharides exhibited antioxidant activities in a concentration-dependent manner. Among three polysaccharides, GLP-F and GLP-V had the higher scavenging effects on hydroxyl radicals, superoxide radicals, DPPH free radical, and stronger reducing power than GLP-H. GLP-F showed the stronger antioxidant capacity than GLP-V and GLP-H in FRAP system. GLP-H and GLP-V showed an almost identical pattern in FRAP system (p > 0.05). However, GLP-V showed the stronger radical scavenging activities than GLP-H. Available data obtained in in vitro models suggested that vacuum freeze drying was an appropriate and effective treatment for obtaining the polysaccharide from G. lucidum. © 2011 Elsevier Ltd. All rights reserved.


Xiao Q.,Jiangnan University | Tong Q.,Jiangnan University | Lim L.-T.,University of Guelph
Carbohydrate Polymers | Year: 2012

Rheological properties of pullulan, sodium alginate and blend solutions were studied at 20 °C, using steady shear and dynamic oscillatory measurements. The intrinsic viscosity of pure sodium alginate solution was 7.340 dl/g, which was much higher than that of pure pullulan (0.436 dl/g). Pure pullulan solution showed Newtonian behavior between 0.1 and 100 s-1 shear rate range. However, increasing sodium alginate concentration in pullulan-alginate blend solution led to a shear-thinning behavior. The effect of temperature on viscosities of all solutions was well-described by Arrhenius equation. Results from dynamical frequency sweep showed that pure sodium alginate and blend solutions at 4% (w/w) polymer concentration were viscoelastic liquid, whereas the pure pullulan exhibited Newtonian behavior. The mechanical properties of pure sodium alginate and pullulan-alginate mixture were analyzed using the generalized Maxwell model and their relaxation spectra were determined. Correlation between dynamic and steady-shear viscosity was analyzed with the empirical Cox-Merz rule. © 2011 Elsevier Ltd. All rights reserved.


Wang B.,Jiangnan University | Xu S.,Jiangnan University | Sun D.-W.,University College Dublin
Food Research International | Year: 2010

In this paper, studies were conducted to determine the odor profile feature of five commercial milk flavorings (three natural milk flavorings and two synthetic flavorings) and one self-made enzyme induced milk flavoring prepared by lipolyzed milk fat. The discrimination of different flavor analysis methods was also evaluated. The ability of an electronic nose consisting of 18 metal oxide semiconductor (MOS) sensors to correlate with the solid phase micro extraction gas chromatography mass spectrometry (SPME-GC-MS) analysis and the sensory test of different milk flavorings was determined. The principal component analysis (PCA) of electronic nose sensor data has been compared with the data provided by GC-MS and sensory analysis. The results indicate that the electronic nose sensors can clearly and rapidly distinguish the difference among the synthetic milk flavorings, the natural milk flavorings and the enzyme induced milk flavoring. And the discrimination among different natural milk flavorings, which were easily confused by sensory tests, was also well performed by the electronic nose. © 2009 Elsevier Ltd. All rights reserved.


Wang X.,Jiangnan University
Nanjing Li Gong Daxue Xuebao/Journal of Nanjing University of Science and Technology | Year: 2014

The decentralized quickest change detection is a common decision problem in the sensor network application. Being different from the classic quickest change detection based on the cumulative sum statistics, an idea of the decentralized quickest change detection based on the cluster analysis is proposed, and an implementation empirically feasible is designed. The implementation comes from the equivalence between K-means clustering and principal component analysis. The implementation does not require pre-change and post-change probability density function of the sensor observation values and the sensors' local computing in advance, and numerical simulations show that it has a smaller detection delay and a smaller probability of false alarm.


Shi F.,Jiangnan University | Jiang J.,Jiangnan University | Li Y.,Jiangnan University | Xie Y.,Jiangnan University
Journal of Industrial Microbiology and Biotechnology | Year: 2013

γ-Aminobutyric acid (GABA), a non-protein amino acid, is a bioactive component in the food, feed and pharmaceutical fields. To establish an effective single-step production system for GABA, a recombinant Corynebacterium glutamicum strain co-expressing two glutamate decarboxylase (GAD) genes (gadB1 and gadB2) derived from Lactobacillus brevis Lb85 was constructed. Compared with the GABA production of the gadB1 or gadB2 single-expressing strains, GABA production by the gadB1-gadB2 co-expressing strain increased more than twofold. By optimising urea supplementation, the total production of l-glutamate and GABA increased from 22.57 ± 1.24 to 30.18 ± 1.33 g L-1, and GABA production increased from 4.02 ± 0.95 to 18.66 ± 2.11 g L-1 after 84-h cultivation. Under optimal urea supplementation, l-glutamate continued to be consumed, GABA continued to accumulate after 36 h of fermentation, and the pH level fluctuated. GABA production increased to a maximum level of 27.13 ± 0.54 g L-1 after 120-h flask cultivation and 26.32 g L-1 after 60-h fed-batch fermentation. The conversion ratio of l-glutamate to GABA reached 0.60-0.74 mol mol-1. By co-expressing gadB1 and gadB2 and optimising the urea addition method, C. glutamicum was genetically improved for de novo biosynthesis of GABA from its own accumulated l-glutamate. © 2013 Society for Industrial Microbiology and Biotechnology.


Chen W.,Jiangnan University
Tien Tzu Hsueh Pao/Acta Electronica Sinica | Year: 2016

The truncating Fourier and continue wavelet representation of a discontinuous piecewise smooth signal will introduce an unneglectable error which was named as the Gibbs phenomenon. In this paper, we proposed an effective piecewise smooth signal approximation method. Firstly, a set of normal orthogonal piecewise polynomials was constructed according to the given positions of breaking points, and it has the properties of orthogonality, convergence and reproduction. Then the signal was orthogonal decomposed under this basis and the best square approximation result could be obtained using reconstruction. The numerical experiments show that our method have the higher accuracy approximation results than the other basis. © 2016, Chinese Institute of Electronics. All right reserved.


Tang X.,Jiangnan University | Wu Q.,Jiangnan University | Le G.,Jiangnan University | Shi Y.,Jiangnan University
Nutrition | Year: 2012

Objective: The present study identified the effects of heat oxidation on protein carbonyl content and α,α-diphenyl-β-picrylhydrazyl (DPPH) free radical-scavenging activity in soy protein. The changes on antioxidant status in male mice fed a heat-oxidized diet were also investigated. Methods: Soy protein heated at 100°C for 30, 60, and 90 min was used to determine the protein carbonyl content and DPPH free radical-scavenging activity in vitro. Male KM mice (3 wk old) were fed a normal diet, an oxidized diet (HD) containing 12% heat-oxidized soy protein, or an HD supplemented with 0.1% lipoic acid. After 4 wk of feeding, apparent digestibility, reactive oxygen species, malondialdehyde, and total antioxidant capacity levels were measured. The antioxidant enzyme activities in serum and tissues were also assayed. Results: Heat-oxidized soy protein showed a significant increase in protein carbonyl formation and a decrease in DPPH free radical-scavenging activity. The HD induced a significant decrease in food intake and apparent digestibility of dry matter and crude protein in mice. Increased levels of reactive oxygen species and malondialdehyde in serum and tissues accompanied by decreased total antioxidant capacity and antioxidant enzyme activities were also observed in HD-fed mice. These changes were partly restored in the lipoic acid-treated group. Conclusion: Heat-oxidized soy protein showed a relatively higher protein carbonyl content and a loss of its free radical-scavenging activity in vitro. The heat oxidation also led the soy protein to generate reactive oxygen species, decrease the antioxidant status, and induce redox imbalance in vivo. The heat oxidation of food protein could be a potential health risk in humans. © 2012 Elsevier Inc.


Xin Y.,Jiangnan University | Zhang M.,Jiangnan University | Adhikari B.,RMIT University
International Journal of Refrigeration | Year: 2014

The application of ultrasound during immersion freezing of broccoli was studied and particular attention was given to the effects on freezing time, microstructure, firmness and drip loss of broccoli. Broccoli florets were immersion frozen in an ultrasound-assisted freezer at two frequencies and four different power levels. The results showed that the total freezing time and times required for pre-cooling, phase change and sub-cooling stages of broccoli were significantly reduced by the application of ultrasound-assisted freezing (UAF) at 150 (30 kHz) or 175 W (20 kHz) power level and with judicious combination of process parameters (exposure time, ultrasound irradiation temperature and pulse mode). The microstructure and the firmness of broccoli tissue were better preserved and the drip loss was significantly reduced by the application of optimized UAF compared to the normal immersion freezing. These findings indicate that there is a great potential of UAF in immersion freezing of food. © 2014 Elsevier Ltd and IIR. All rights reserved.


He S.,Anhui University | Liu F.,Jiangnan University
Neurocomputing | Year: 2013

The stochastic finite-time boundedness (FTB) problem is considered for a class of Markovian jumping neural networks (MJNNs) with time delay and uncertainties. By selecting the appropriate stochastic Lyapunov-Krasovskii functional, sufficient conditions of stochastic FTB of MJNNs are presented and proved. The FTB criteria are formulated in the form of linear matrix inequalities. Simulation results illustrate the effectiveness of the developed approaches. © 2012 Elsevier B.V.


Cheng X.-F.,Jiangnan University | Zhang M.,Jiangnan University | Adhikari B.,RMIT University | Islam M.N.,Jiangnan University | Xu B.-G.,Jiangnan University
International Journal of Refrigeration | Year: 2014

In this study, the effect of ultrasound irradiation temperature and ultrasound intensity on the freezing and nucleation in strawberry samples was studied. The application of ultrasound irradiation at different temperatures was able to induce nucleation at lower degree of supercooling compared to the control samples. The achieved degree of supercooling in the ultrasound irradiated strawberries was linearly correlated to the ultrasound irradiation temperature. At the ultrasound irradiation temperature of -1.6 °C, the characteristic freezing time (CFT) was significantly shorter than that in the control sample (p < 0.05). The application of ultrasound at higher intensities was found to effectively shorten the CFT. The degree of supercooling in ultrasound irradiated samples was not linearly correlated to ultrasound intensity. In conclusion, the combination of ultrasound irradiation temperature and intensity can be effective in controlling nucleation and freezing processes of perishable fruits such as strawberry.©2014 Elsevier Ltd and IIR. All rights reserved.


Ding F.,Jiangnan University | Ding J.,Jiangnan University
International Journal of Adaptive Control and Signal Processing | Year: 2010

This paper considers the problems of parameter identification and output estimation with possibly irregularly missing output data, using output error models. By means of an auxiliary model (or reference model) approach, we present a recursive least-squares algorithm to estimate the parameters of missing data systems, and establish convergence properties for the parameter and missing output estimation in the stochastic framework. The basic idea is to replace the unmeasurable inner variables with the output of an auxiliary model. Finally, we test the effectiveness of the algorithm with an example system. Copyright © 2009 John Wiley & Sons, Ltd.


Min R.,Jiangnan University
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2013

To reveal the correlation between thermostability of xylanase EvXyn11(TS) and its N-terminal disulfide bridge, an EvXyn11(TS)-encoding gene (Syxyn11) was synthesized and subjected to site-directed mutagenesis. Multiple homology alignment of protein primary structures between the EvXyn11(TS) and several GH family 11 xylanases displayed that, in their N-termini, only EvXyn11(TS) contained a disulfide bridge (Cys5-Cys32), whose effect on the xylanase thermostability was predicted by molecular dynamics simulation. We constructed a gene Syxyn11(M), encoding the mutated xylanase (EvXyn11(M)) without N-terminal disulfide bridge. Then, Syxyn11 and Syxyn11(M) were expressed in Pichia pastoris GS115, and temperature and pH properties of the expressed enzymes were analyzed. The analytical results displayed that the temperature optimum of EvXyn11(M) was 70 degrees C, which was 15 degrees C lower than that of EvXyn11(TS). The half-life (t1/2(90)) of EvXyn11(TS) at 90 degrees C was 32 min, while the t1/2(70) of EvXyn11(M) at 70 degrees C was only 8.0 min. The important role of the N-terminal disulfide bridge on the thermostability of EvXyn11(TS) was first predicted by molecular dynamics simulation, and confirmed by site-directed mutagenesis. This work provided a novel strategy to improve thermostabilities of the mesophilic family 11 xylanases with high specific activities.


He S.,Jiangnan University | Liu F.,Jiangnan University
Journal of Systems Engineering and Electronics | Year: 2010

The robust H ∞ control problems for stochastic fuzzy neutral Markov jump systems (MJSs) with parameters uncertainties and multiple time-delays are considered. The delays are respectively considered as constant and time varying, and the uncertain parameters are assumed to be norm bounded. By means of Takagi-Sugeno fuzzy models, the overall closed-loop fuzzy dynamics are constructed through selected membership functions. By selecting the appropriate Lyapunov-Krasovskii functions, the sufficient condition is given such that the uncertain fuzzy neutral MJSs are stochastically stability for all admissible uncertainties and satisfies the given H ∞ control index. The stability and H ∞ control criteria are formulated in the form of linear matrix inequalities, which can be easily checked in practice. Practical examples illustrate the effectiveness of the developed techniques.


Liang C.,Jiangnan University | Wang W.,Jiangnan University
Environmental Science and Pollution Research | Year: 2013

Excess of rare earth elements in soil can be a serious environmental stress on plants, in particular when acid rain coexists. To understand how such a stress affects plants, we studied antioxidant response of soybean leaves and roots exposed to lanthanum (0.06, 0.18, and 0.85 mmol L-1) under acid rain conditions (pH 4.5 and 3.0). We found that low concentration of La3+ (0.06 mmol L-1) did not affect the activity of antioxidant enzymes (catalase and peroxidase) whereas high concentration of La3+ (≥0.18 mmol L-1) did. Compared to treatment with acid rain (pH 4.5 and pH 3.0) or La3+ alone, joint stress of La3+ and acid rain affected more severely the activity of catalase and peroxidase, and induced more H2O2 accumulation and lipid peroxidation. When treated with high level of La3+ (0.85 mmol L-1) alone or with acid rain (pH 4.5 and 3.0), roots were more affected than leaves regarding the inhibition of antioxidant enzymes, physiological function, and growth. The severity of oxidative damage and inhibition of growth caused by the joint stress associated positively with La3+ concentration and soil acidity. These results will help us understand plant response to joint stress, recognize the adverse environmental impact of rare earth elements in acidic soil, and develop measures to eliminate damage caused by such joint stress. © 2013 Springer-Verlag Berlin Heidelberg.


Abbas S.,Jiangnan University | da Wei C.,Jiangnan University | Hayat K.,Quaid-i-Azam University | Xiaoming Z.,Jiangnan University
Food Reviews International | Year: 2012

Developments in nutritional sciences have increased the functional significance of ascorbic acid (vitamin C) as a food component in the human diet for health promotion and disease prevention. This has motivated food researchers to develop ascorbic acid-fortified food products to deliver appropriate levels of this vital food ingredient. Unfortunately, the highly unstable nature of ascorbic acid has posed technological challenges for its incorporation into different food systems. Microencapsulation is a promising approach to ensure the stability of ascorbic acid and to improve consumer acceptability towards the carrier food. The most commonly used techniques for ascorbic acid (water soluble) encapsulation, including spray drying, spray cooling, spray chilling, fluidized bed coating, liposomes, and extrusion, are reviewed and discussed with respect to technical hurdles and potential benefits. © 2012 Copyright Taylor and Francis Group, LLC.


Li Z.J.,Jiangnan University | Xia Q.f.,Jiangnan University
Reviews in Analytical Chemistry | Year: 2012

The emergence of graphene (GR) has recently opened up an exciting new fi eld in the science and technology of twodimensional (2D) nanomaterials with continuously growing academic and technological impetus. GR exhibits unique electronic, optical, magnetic, thermal, and mechanical properties arising from its strictly 2D structure and, thus, has many important technical applications. In the last 2 years, GR, asnovel sensing material has attracted tremendous attention and research interest. To further improve electrochemical property, large numbers of GR-based hybride materials have been welldesigned, synthesized, and investigated for sensing application. The hybridization can be an effective strategy to enhance functionality of the materials, and the integration of nanomaterials on GR potentially paves a new way to enhance their electronic,chemical, and electrochemical properties. As a result, the GR composites often offer better analytical characteristics than GR materials alone. However, the immobilizations of GR-based material and enzyme are important to enhance electrochemical properties and use of the biosensor. Classical coating method results in poor stability due to loss of the enzyme/ antibody. To resolve the problem, we developed a green and controllable strategy to fabricate well-dispersed GR-metal nanocomposite modifi ed functional conducting polymer fi lm containing carbonyl groups with electrochemical deposition. Horseradish peroxidase was fi nally connected covalently to the fi lm with 1-ethyl-3-(3-dimethylaminepropyl) carbodiimide (EDC)/N- hydroxysuccinimide (NHS) as activator. The asprepared GR/gold nanocomposite offers remarkable catalysis performance to the redox of hydrogen peroxide on the electrode surface. In this review, from the viewpoint of chemistry and materials, we will cover recent signifi cant advances in hybrids, design, and synthesis of GR-based fi lm, as well as immobilization of enzyme/antibody on the electrode, and analytical application together with discussion on the major challenges and opportunities for future GR research. © 2012 by Walter de Gruyter.


Zhao J.,Jiangnan University | Zhao J.,Bauhaus University Weimar | Jiang J.-W.,Bauhaus University Weimar | Wang L.,Nanjing University of Aeronautics and Astronautics | And 2 more authors.
Journal of the Mechanics and Physics of Solids | Year: 2014

We develop the coarse-grained (CG) potentials of single-walled carbon nanotubes (SWCNTs) in CNT bundles and buckypaper for the study of the static and dynamic behaviors. The explicit expressions of the CG stretching, bending and torsion potentials for the nanotubes are obtained by the stick-spiral and the beam models, respectively. The non-bonded CG potentials between two different CG beads are derived from analytical results based on the cohesive energy between two parallel and crossing SWCNTs from the van der Waals interactions. We show that the CG model is applicable to large deformations of complex CNT systems by combining the bonded potentials with non-bonded potentials. Checking against full atom molecular dynamics calculations and our analytical results shows that the present CG potentials have high accuracy. The established CG potentials are used to study the mechanical properties of the CNT bundles and buckypaper efficiently at minor computational cost, which shows great potential for the design of micro- and nanomechanical devices and systems. © 2014 Elsevier Ltd.


Gu Z.-G.,Jiangnan University | Gu Z.-G.,Nanjing University | Xu X.-X.,Jiangnan University | Zhou W.,Jiangnan University | And 3 more authors.
Chemical Communications | Year: 2012

A fascinating polythreaded coordination network formed by 1D crankshaft shaped chains threading into a 2D undulated sheet in a one-over/one-under interweaving fashion was reported, in which the 2D layer exhibits an unusual polyknotted entanglement containing triple-stranded molecular braids. © The Royal Society of Chemistry 2012.


Hu A.,Jiangnan University | Xu Z.,Jiangnan University | Guo L.,Jiangnan University
Chaos | Year: 2010

The paper studies the existence of generalized synchronization in complex networks, which consist of chaotic systems. When a part of modified nodes are chaotic, and the others have asymptotically stable equilibriums or orbital asymptotically stable periodic solutions, under certain conditions, the existence of generalized synchronization can be turned to the problem of contractive fixed point in the family of Lipschitz functions. In addition, theoretical proofs are proposed to the exponential attractive property of generalized synchronization manifold. Numerical simulations validate the theory. © 2010 American Institute of Physics.


Jia Y.,Jiangnan University | Yu K.,Jiangnan University | Qian K.,Jiangnan University
Nanoscale Research Letters | Year: 2013

A facile approach was developed to prepare multi-walled carbon nanotubes/graphene nanoplatelets hybrid materials through covalent bond formation. First, poly(acryloyl chloride) was grafted onto oxidized multi-walled carbon nanotubes through the reaction between the acyl chloride groups of poly and the hydroxyl groups of oxidized multi-walled carbon nanotubes. Second, the remaining acyl chloride groups of poly were allowed to react with the hydroxyl groups of hydroxylated graphene nanoplatelets. Scanning electron microscopy and transmission electron microscopy data showed that the multi-walled carbon nanotubes and graphene nanoplatelets were effectively connected with each other. And Fourier transform infrared spectroscopy data indicated the formation of covalent bonds between carbon nanotubes and graphene nanoplatelets. Conformational changes were monitored by Raman spectroscopy. This novel kind of carbon hybrid materials may have the potential application in a wide field, especially in increasing the toughness and strength of the matrix resin. © 2013 Jia et al.


Ji W.,University of Minnesota | Panus D.,University of Minnesota | Palumbo R.N.,University of Minnesota | Tang R.,Jiangnan University | Wang C.,University of Minnesota
Biomacromolecules | Year: 2011

Poly(2-aminoethyl methacrylate) (PAEM) homopolymers with defined chain length and narrow molecular weight distribution were synthesized using atom transfer radical polymerization (ATRP), and a comprehensive study was conducted to evaluate the colloidal properties of PAEM/ plasmid DNA polyplexes, the uptake and subcellular trafficking of polyplexes in antigen-presenting dendritic cells (DCs), and the biological performance of PAEM as a potential DNA vaccine carrier. PAEM of different chain length (45, 75, and 150 repeating units) showed varying strength in condensing plasmid DNA into narrowly dispersed nanoparticles with very low cytotoxicity. Longer polymer chain length resulted in higher levels of overall cellular uptake and nuclear uptake of plasmid DNA, but shorter polymer chains favored intracellular and intranuclear release of free plasmid from the polyplexes. Despite its simple chemical structure, PAEM transfected DCs very efficiently in vitro in media with or without serum and led to phenotypic maturation of DCs. When a model antigen-encoding ovalbumin plasmid was used, transfected DCs stimulated the activation of nai ̈ve CD8+ T cells to produce high levels of interferon-γ. The efficiency of transfection, DC maturation, and CD8+ T cell activation showed varying degrees of polymer chainlength dependence. These structurally defined cationic polymers may have much potential as efficient DNA vaccine carriers and immunostimulatory adjuvants. They may also serve as a model material system for elucidating structural and intracellular mechanisms of polymer-mediated DNA vaccine delivery. © 2011 American Chemical Society.


Chen A.-J.,Jiangnan University | Chen A.-J.,Key Laboratory of Food Packaging Techniques and Safety of China National Packaging Corporation
Mathematical Problems in Engineering | Year: 2013

The coupled nonlinear dynamical equations were developed for a tilted spring packaging system with critical components. The approximate solution and resonance conditions of system were obtained applying a variational iteration method. The resonance conditions, which should be avoided in the packaging design, can be easily obtained by VIM. © 2013 An-Jun Chen.


Li X.,Jiangnan University | Ding R.,Jiangnan University | Zhou L.,Jiangnan University
International Journal of Computer Mathematics | Year: 2013

This paper focuses on identification problems for Hammerstein systems with non-uniform sampling. By using the over-parameterization technique, we derive a linear regressive identification model with different input updating rates. To solve the identification problem of Hammerstein output error systems with the unmeasurable variables in the information vector, the least-squares-based iterative algorithm is presented by replacing the unmeasurable variables with their corresponding iterative estimates. The performances of the proposed algorithm are analysed and compared by using a numerical example. © 2013 Copyright Taylor and Francis Group, LLC.


Islam M.N.,Jiangnan University | Zhang M.,Jiangnan University | Adhikari B.,RMIT University | Xinfeng C.,Jiangnan University | Xu B.-G.,Jiangnan University
International Journal of Refrigeration | Year: 2014

The application of power ultrasound during immersion freezing significantly (P < 0.05) affected freezing rate of mushrooms. Ultrasound at 0.39 W cm -2 (20 kHz) reduced nucleation time by 24%, 53% and 34% in Lentinulaedodes, Agaricusbisporus and Pleurotuseryngii respectively. Drip losses during the thawing process were found to be 10% less compared to control samples. Whiteness index increased when ultrasound intensity was higher than 0.27 W cm-2 (20 kHz) in L. edodes and P. eryngii whereas, ultrasound at 0.39 W cm-2 (20 kHz) showed the highest chroma value with lower yellowness and browning indices. Ultrasound at 0.39 W cm-2 (20 kHz) resulted in the highest textural hardness values in all the three mushroom varieties. Polyphenol oxidase and peroxidase enzyme activities were also significantly (P < 0.05) reduced with the increase in ultrasound power during ultrasound-assisted freezing (UAF). These results indicated that UAF can be a suitable technology for industrial freezing of mushroom. © 2014 Elsevier Ltd and IIR. All rights reserved.


Xu B.,Jiangnan University | Zhang M.,Jiangnan University | Bhandari B.,University of Queensland | Cheng X.,Jiangnan University
International Journal of Refrigeration | Year: 2014

The effect of ultrasound-assisted immersion freezing on the dynamic nucleation of ice and the delay from ultrasound application onset to nucleation commencement of radish cylinder samples were studied. The samples were frozen in 30% (w v-1) CaCl2 solution (-20 °C) in an ultrasonic bath system. To evaluate nucleation, ultrasound irradiation (20 kHz) was carried out with different durations (0 s, 3 s, 7 s, 10 s or 15 s), onset temperature (-0.5, -1, -1.5 and -2°C) and intensities (0.09, 0.17, 0.26 and 0.37 W cm-2). The results showed that ultrasound irradiation was able to induce nucleation and the nucleation temperature of radish cylinder samples exhibited a good fit to linear equation with the ultrasound irradiation temperature under 7 s duration and 0.26 W cm-2 intensity. Ultrasound irradiation temperature at -0.5°C for 7 s duration with intensity of 0.26 W cm-2, was an optimal ultrasound application conditions for the nucleation inducement of radish cylinder samples. The results of the current study implied that ultrasound offered promising application to control the crystallization process in freezing of solid foods. ©2014 Elsevier Ltd. All rights reserved.


Duan X.,Jiangnan University | Chen J.,Jiangnan University | Wu J.,Jiangnan University
Bioresource Technology | Year: 2013

In this study, the effects of temperature, IPTG (Isopropyl β-d-1-thiogalactopyranoside) concentration, and osmolytes (proline, K-glutamate, and betaine) on cell growth and soluble pullulanase productivity of recombinant Escherichia coli were investigated. The yield of soluble pullulanase was found to be enhanced with decrease in cultivation temperature, lower IPTG concentration, and betaine supplementation in a shake flask. In addition, a modified two-stage feeding strategy was proposed and applied in a 3-L fermentor supplied with 20mM betaine, which achieved a dry cell weight of 59.3gL-1. Through this cultivation approach at 25°C, the total soluble activity of pullulanase reached 963.9UmL-1, which was 8.3-fold higher than that observed without addition of betaine at 30°C (115.8UmL-1). The higher expression of soluble pullulanase in a scalable semisynthetic medium showed the potential of the proposed process for the industrial production of soluble enzyme. © 2013 Elsevier Ltd.


Chang G.,Jiangnan University | Chang G.,Wilmar Biotechnology Research and Development Center Co. | Luo Z.,Wilmar Biotechnology Research and Development Center Co. | Gu S.,Wilmar Biotechnology Research and Development Center Co. | And 3 more authors.
Bioresource Technology | Year: 2013

DHA production by Schizochytrium sp. S31 was studied in batch cultures on glycerol with stepwise dissolved oxygen strategy. Three growth stages were identified as cell growth, lipid accumulation and lipid turnover. It was revealed that fatty acid (FA) shifts during the three growth stages involved the activity changes of glycerol kinase (GK), FAD+-dependent glycerol-3-phosphate dehydrogenase (FAD+-G-3-PDH), malic enzyme (ME), ATP citrate lyase (ACL) and NAD+-dependent isocitrate dehydrogenase (NAD+-ICDH). Glycerol dissimilation in Schizochytrium sp. S31 was suggested via a phosphorylation by GK and a following oxidation by FAD+-G-3-PDH. Lipid accumulation of this strain was a growth-associated process, but the assimilable nitrogen depletion enhanced the accumulation of lipids. The exhaustion of glycerol induced the lipid turnover stage, where the short chain fatty acids were preferentially degraded and converted into lipid-free biomass (Xf) which was correlated to the increase of DHA content in biomass. © 2013 Elsevier Ltd.


Lu J.,Jiangnan University | Lin Q.,Jiangnan University | Li Z.,Jiangnan University | Rohani S.,University of Western Ontario
Journal of Chemical and Engineering Data | Year: 2012

In this work the solubility of l-phenylalanine anhydrous and monohydrate forms in pure water, a water + acetone mixture, and a water + ethanol mixture at various temperatures were measured systematically using the gravimetric method as well as predicted using the universal quasichemical (UNIQUAC) model. Generally the solubility increased with the temperature and decreased with an increase in the content of acetone or ethanol in the solvent mixtures. The two forms were found to be enantiotropically related, and the transition temperature was about 308.9 K. Besides, the UNIQUAC model was demonstrated to work well for the prediction of the solubility of this pseudopolymorphic system in water and its binary mixtures with a low content of organic solvent. © 2012 American Chemical Society.


Chen S.,Jiangnan University | Chen S.,Anhui Science and Technology University | Zhang M.,Jiangnan University | Wang S.,Washington State University
Postharvest Biology and Technology | Year: 2011

Experiments of initial hermetic sealing using high barrier film were carried out on 'Kyoho' grapes (Vitis vinifera L.×V. Labrusca L. cv. Kyoho) in the 2008 and 2009 fruit seasons, to investigate their potential to enhance quality and extend storage life of the fruit. In the 2008 season, grapes were packaged in high barrier film bags for 1, 2, 3, 4 and 5 weeks, and a modified atmosphere (MA) of low oxygen and high carbon dioxide was formed after sealing. After packaging, fruit were removed from bags and stored in air for up to 90d at 0°C. In the 2009 season, grapes were packaged in perforated bags, or in high barrier film bags for 2 weeks and subsequently perforated bags to avoid further anoxia and excessive CO2 accumulation. After treatment, fruit were stored for up to 90d at 0°C, followed by shelf-life at 20°C for 7d. Non-packaging air storage was used as a control in both seasons. Fruit quality attributes including soluble solids, titratable acidity, stem browning, berry drop and decay incidence were measured. The results indicated that short-term initial MAP (≤2 weeks) had potential for improving appearance of bunches and maintaining the quality of berries during long-term storage, and significantly reduced quality deterioration. Stems were greener and berry drop and decay incidence were more effectively controlled when fruit were sealed in high barrier film bags for 2 weeks and the bags were subsequently perforated. © 2010 Elsevier B.V.


Feng Y.F.,Jiangnan University | Zhang M.,Jiangnan University | Jiang H.,Jiangnan University | Sun J.C.,Haitong Food Group Company
Drying Technology | Year: 2012

Microwave-assisted spouted bed (MSBD) drying of lettuce cubes was investigated experimentally. Response surface methodology was used to optimize the process with spouting air temperature, microwave power level, and superficial air velocity. The dried product obtained was compared with that obtained using other drying technologies such as hot air drying, air spouted bed drying, vacuum microwave drying, and vacuum freeze drying. The comparison is based on the rehydration ratio, chlorophyll content of the product, color, and the drying time required. © 2012 Copyright Taylor and Francis Group, LLC.


Wang Y.,Jiangnan University | Cao Y.,Jiangnan University | Fang C.,Jiangnan University | Gong Q.,Jiangnan University
Analytica Chimica Acta | Year: 2010

A selective, sensitive, rapid and reliable method based on molecularly imprinted polymers (MIPs) with dual templates to determine total content of parabens in cosmetics was developed. With methylparaben (MP) and propylparaben (PP) as dual-templates, methacrylic acid (MAA) as a functional monomer and tripropylene glycol diacrylate (TPGDA) as a cross-linker, MIPs film on a glassy carbon electrode was constructed as paraben sensor. At oxidation potential of 0.94. V (vs. SCE), the peak currents on the MIPs sensor were proportional to the concentration of parabens with square wave voltammetry. As the ratio of MP to PP in the MIPs was 1:1.25, the regression equations for four parabens were almost the same. The linear range was 20-100 μM for MP and EP, 5-100 μM for PP, and 5-80 μM for BP, with detection limit of 0.4 μM for MP and EP, 0.2 μM for the others. The total content of parabens could be calculated according to the average of these four regress equations. At least 10 times of structural analogs, such as p-hydroxybenzoic acid, p-aminobenzoic acid and phenol would not interfere with the determination of parabens. Nonanalogous coexistences such as vitamin C had no response on the sensor at all. Rapid response of the MIPs sensor was obtained within 1. min. MIPs sensor had been used to determine total content of parabens in cosmetic samples with recoveries between 98.7% and 101.8%. It reveals that the MIPs sensor with multi-templates has a potential to determine the total content of a group of homologous compounds. © 2010 Elsevier B.V.


He R.,Jiangnan University | He R.,University of Manitoba | Girgih A.T.,University of Manitoba | Malomo S.A.,University of Manitoba | And 2 more authors.
Journal of Functional Foods | Year: 2013

In this study, rapeseed protein isolate was hydrolyzed with various proteases to obtain hydrolysates that were separated by membrane ultrafiltration into four molecular size fractions (<1, 1-3, 3-5, and 5-10. kDa). Alcalase hydrolysis significantly (p< 0.05) produced the highest yield of protein hydrolysate while Flavourzyme produced the least. The <1. kDa fraction was the most abundant after the membrane ultrafiltration of the protein hydrolysates, which indicates that the proteases were efficient at reducing the native rapeseed proteins into low molecular weight peptides. Antioxidant properties of the resulting hydrolysates and membrane fractions were characterized and results showed the Pepsin + Pancreatin (P + P) protein hydrolysate had significantly highest (p< 0.05) scavenging activity against DPPH radical among the unfractionated enzymatic hydrolysates. But the P + P hydrolysate was not as effective as other hydrolysates during long-term inhibition of linoleic acid oxidation. For most of the samples, fractionation into the <1. kDa peptides significantly (p< 0.05) improved DPPH and superoxide scavenging properties when compared to the unfractionated protein hydrolysates. Only the <1. kDa fraction showed ferric reducing antioxidant power and the effect was dose-dependent. Overall, Alcalase and Proteinase K seem to be more efficient proteases to release antioxidant peptides from rapeseed proteins when compared to P + P, Flavourzyme and Thermolysin. © 2012 Elsevier Ltd.


Yin Y.,Jiangnan University | Wang C.,Jiangnan University
Journal of Sol-Gel Science and Technology | Year: 2012

This study concerns the organic-inorganic hybrid coating of silica sol based on dyed cotton, silk and wool fabrics in order to increase the repellence to capsicum oil via adding methyltriethoxysilane, octyltriethoxysilane, hexadec-ltrimethoxysilane or tridecafluorooctyltriethoxysilane (FAS) in the inorganic silica sol. The dyed cotton fabric treated with hybrid silica sol doped with FAS (F-silica sol, FAS 4 %) presents oil-repellent capability, and the contact angles of capsicum oil on the treated cotton, silk and wool fabrics are 98.5, 111.59 and 122.15, respectively. A high FAS concentration (20 %) can improve the oil-repellent ability to 5 grades comparing to the untreated fabrics. The color strengths (K/S) of the coated fabrics change slightly, while the maximum absorption wavelengths of the coated fabrics are the same as the untreated fabrics. Although the drape coefficient of cotton fabric is increased to 54 % from 39 % after coated with F-silica sol, the effect is not significant. Compared to the weight gain rate of untreated cotton, silk and wool samples (1.89, 1.23 and 2.38 %), the weight gain rate of the cotton, silk and wool samples coated with F-silica sol are 6.99, 4.76 and 7.69 %, respectively. The calculated sol-gel weight gains (5.10, 3.53 and 5.31 %) of coated fabrics indicate that the silica coating is subsistent on the fiber surfaces. © 2012 Springer Science+Business Media New York.


Luo J.,Jiangnan University | Jiang S.,Jiangnan University | Liu X.,Jiangnan University
Sensors and Actuators, B: Chemical | Year: 2014

A highly selective and sensitive electrochemical sensor for bovine hemoglobin (BHb) was developed based on a novel graphene-molecular imprinted polymers composite (GR-MIP) as recognition element. The GR-MIP composite was synthesized by oxide self-polymerization of dopamine on the surface of graphene in the presence of template molecules (BHb) in aqueous solution, which is a one-pot facile and green preparation procedure. The fabrication process of the GR-MIP sensor was characterized by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS), in which [Fe(CN)6] 3-/4- was used as an electrochemical active probe. The as-prepared GR-MIP sensor was tested by DPV to investigate the relationship between the current response and BHb concentration. Under optimized experimental conditions, selective detection of BHb in a concentration range of 1.0 × 10 -9 mg mL-1 to 1.0 × 10-1 mg mL -1 with detection limit of 2.0 × 10-10 mg mL -1 was obtained, which outperformed the most of reported BHb detecting methods. This novel GR-MIP sensor also exhibited excellent selectivity and good reproducibility, and was used to the determination of BHb in real samples with satisfactory results. The outstanding sensing behavior of the target protein coupled to its low production cost and facile, green preparation procedure makes the GR-MIP sensor attractive in the sensitive and selective detection of target proteins in biological fluids. © 2014 Elsevier B.V. All rights reserved.


Tong Q.,Jiangnan University | Xiao Q.,Jiangnan University | Lim L.-T.,University of Guelph
International Journal of Food Science and Technology | Year: 2013

The effects of glycerol, sorbitol, xylitol and fructose plasticisers on water sorption, mechanical properties, water vapour permeability (WVP) and microstructure of pullulan-alginate-carboxymethycellulose (PAC) blend films were investigated. At low plasticiser concentrations (below 7% w/w dry basis), antiplasticisation effect was observed, causing an increase in tensile strength (TS) but a decrease in the equilibrium moisture content. As glycerol concentration increased from 0% to 7%, TS increased from 68.1 to 69.6 MPa, whereas equilibrium moisture contents at 0.84 aw decreased from 0.37 to 0.3 g H2O g-1 dry basis. At higher plasticiser concentrations (14-25% w/w), an opposite trend was observed on the PAC films, resulting in the reduction of TS and elevation of moisture content. Among the four plasticisers tested, the fructose-plasticised films were the most brittle, showing the highest TS, but had the lowest elongation at break (EAB), WVP and equilibrium moisture content values than films plasticised with other polyols. On the other hand, glycerol resulted in the most flexible film structure, exhibiting opposite materials' properties as compared with the fructose-plasticised films. For instance, at 25% (w/w) plasticiser concentration, EAB and WVP values of fructose-plasticised films were 33.5% and 3.48 × 10-6 g m Pa-1 h-1 m-2, which were significantly lower than that of glycerol-plasticised films (58.6% and 4.86 × 10-6 g m Pa-1 h-1 m-2, respectively). Scanning electron microscopy showed that the plasticised PCA films were less homogeneous and more porous than the unplasticised counterparts, indicating that plasticisers had an effect on the microstructural morphology of the film matrix. © 2012 The Authors. International Journal of Food Science and Technology © 2012 Institute of Food Science and Technology.


Xu G.,Jiangnan University | Chen X.,Jiangnan University | Liu L.,Jiangnan University | Jiang L.,Jiangnan University
Bioresource Technology | Year: 2013

In this study, the simultaneous use of reductive and oxidative routes to produce fumaric acid was explored. The strain FMME003 (Saccharomyces cerevisiae CEN.PK2-1C. δTHI2) exhibited capability to accumulate pyruvate and was used for fumaric acid production. The fum1 mutant FMME004 could produce fumaric acid via oxidative route, but the introduction of reductive route derived from Rhizopus oryzae NRRL 1526 led to lower fumaric acid production. Analysis of the key factors associated with fumaric acid production revealed that pyruvate carboxylase had a low degree of control over the carbon flow to malic acid. The fumaric acid titer was improved dramatically when the heterologous gene RoPYC was overexpressed and 32. μg/L of biotin was added. Furthermore, under the optimal carbon/nitrogen ratio, the engineered strain FMME004-6 could produce up to 5.64. ±. 0.16. g/L of fumaric acid. These results demonstrated that the proposed fermentative method is efficient for fumaric acid production. © 2013 Elsevier Ltd.


Yang L.-B.,Jiangnan University | Zhan X.-B.,Jiangnan University | Zheng Z.-Y.,Jiangnan University | Wu J.-R.,Jiangnan University | And 2 more authors.
Bioresource Technology | Year: 2013

The effect of osmotic pressure on erythritol and mannitol production by an osmophilic yeast strain of Yarrowia lipolytica CICC 1675 using glycerol as the sole carbon source was investigated. Appropriately high osmotic pressure was found to enhance erythritol production and inhibit mannitol formation. A novel two-stage osmotic pressure control fed-batch strategy based on the kinetic analysis was developed for higher erythritol yield and productivity. During the first 96h, the osmotic pressure was maintained at 4.25osmol/kg by feeding glycerol to reduce the inhibition of cell growth. After 132h, the osmotic pressure was controlled at 4.94osmol/kg to maintain a high dpery/dt. Maximum erythritol yield of 194.3g/L was obtained with 0.95g/L/h productivity, which were 25.7% and 2.2%, respectively, improvement over the best results in one-stage fed-batch fermentation. This is the first report that a novel osmotic pressure control fed-batch strategy significantly enhanced erythritol production. © 2013 Elsevier Ltd.


Liu Y.,Jiangnan University | Ren X.,Jiangnan University | Huang T.S.,Auburn University
Applied Surface Science | Year: 2014

The monomer (3-acrylamidopropyl)trimethylammonium chloride (APTMAC) was used to treat cotton fibers by grafting copolymerization. The grafted cotton fabrics were characterized by SEM image and FTIR spectra. The treated samples with quaternary ammonium groups could decrease 96.08% of Staphylococcus aureus and 48.74% of Escherichia coli O157:H7 within 30 min. After chlorination with dilute sodium hypochlorite, the treated cotton fabrics containing both N-halamine and quaternary ammonium groups effectively inactivated 100% (log reduction 5.82) of S. aureus and 100% (log reduction 6.26) of E. coli O157:H7 within 5 min of contact time. The grafting process of cotton fabric has small effect on the thermal stability and tensile strength, which favors the practical application. Compared to the traditional pad-dry-cure method to produce antibacterial materials, the radical grafting copolymerization method occurred in water without any organic solvents involved in the whole treatment. © 2014 Elsevier B.V. All rights reserved.


Ni Y.,Jiangnan University | Xia Z.,Jiangnan University | Wang Y.,Jiangnan University | Sun Z.,Jiangnan University
Bioresource Technology | Year: 2013

Corn stover hydrolysate (CSH) and cane molasses were studied for butanol fermentation by Clostridium saccharobutylicum DSM 13864 in continuous fermentation. Using cane molasses as substrate, solvent of 13.75g/L (butanol 8.65g/L) and productivity of 0.439g/L/h were achieved in a four-stage continuous fermentation at a gradient dilution mode of 0.15-0.15-0.125-0.1h-1. In continuous fermentation using CSH as substrate, total solvent titer of 11.43g/L (butanol 7.81g/L) and productivity of 0.429g/L/h were reached at a dilution rate of 0.15h-1, and the steady process was continuously operated for 220h without compromise in solvent titer. © 2012 Elsevier Ltd.


Liu X.-B.,Jiangnan University | Gu Q.-Y.,Jiangnan University | Yu X.-B.,Jiangnan University
Bioresource Technology | Year: 2013

To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2. g/L to 15.3. g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production. © 2012 Elsevier Ltd.


Zhang Y.,Jiangnan University | Li S.,Jiangnan University | Liu L.,Jiangnan University | Wu J.,Jiangnan University
Bioresource Technology | Year: 2013

A new strain, FMME044, exhibited a remarkable ability to synthesize acetoin and was identified as Bacillus amyloliquefaciens. The following characteristics of enzyme activity were found: 2,3-butanediol was reverse transformed to acetoin upon depletion of glucose; lower agitation speeds favored 2,3-butanediol accumulation; and higher agitation speeds favored reverse transformation of 2,3-butanediol to acetoin. In order to enhance acetoin production by manipulating the carbon flux distribution, a two-stage agitation speed control strategy was proposed: during the first 24h, the agitation speed was set to 350rpm to achieve a high 2,3-butanediol concentration and then the speed was increased to 500rpm to reverse transform 2,3-butanediol to acetoin. Following this strategy, a high titer (51.2gL-1), yield (0.43gg-1), and productivity (1.42gL-1h-1) of acetoin were achieved. The results demonstrated that B. amyloliquefaciens FMME044 is a potential industrial strain for acetoin production. © 2012.


Zhu L.,Jiangnan University | Cao Y.,Jiangnan University | Cao G.,Jiangnan University
Biosensors and Bioelectronics | Year: 2014

A selective electrochemical sensor based on magnetic molecularly imprinted nanoparticles was developed for determination of bisphenol A (BPA). The particles with regular morphology, high saturation magnetization and good monodispersion were prepared. The hydrophilicity, sensitivity and anti-fouling of the sensor were enhanced by modifying carbon paste electrode with surfactant CTAB in advanced. The results demonstrated that the response of BPA on imprinted electrode was 2.6 times as much as that on non-imprinted sensor. Moreover, the separation factors of BPA to β-estradiol, estriol and diethylstilbestrol were 16.5, 17.3 and 6.6, respectively. Under optimized conditions, the currents were found to be proportional to the BPA concentrations in the range of 6.0×10-7-1.0×10-4mol/L with a detection limit of 1.0×10-7mol/L (S/N=3). A rapid response of the imprinted sensor was obtained within 3min. The developed sensor was successfully used for determination of BPA in actual samples such as drink bottles and lake water. © 2013 Elsevier B.V.


Liu J.,Jiangnan University | Wang Q.,Jiangnan University | Fan X.R.,Jiangnan University
Journal of Sol-Gel Science and Technology | Year: 2012

A new method for improving the anti-ultraviolet and anti-ageing abilities of wool fabric was reported in this paper. TiO 2 sols and poly (sodium 4-styrene-sulfonate) (PSS) were coated on the wool fibers via layer-by-layer (LBL) electrostatic self-assembly deposition. The morphologies and compositions of TiO 2 sol-coated wool fabrics were characterized using SEM, surface Zeta potential, apparent color depth (K/S), ultraviolet (UV) transmission and alkali solubility. The SEM pictures showed that there were quite a few deposits absorbed on the wool surface. The dyeing depth and Zeta potential presented obvious ''layer-layer alternate vibration'' along with the change of deposited materials, revealing the surface structure of the assembled wool fiber. The results of ultraviolet (UV) transmission and alkali solubility indicated that the modified wool fabrics obtained good anti-ultraviolet and antiageing properties. In addition, the sol-assembled wool fabrics had good washing fastness. The studies proved that the LBL electrostatic self-assembly deposition is a promising way to endow the textiles with surface functionality. © Springer Science+Business Media, LLC 2012.


Li Z.L.,Jiangnan University | Mo L.,Jiangnan University | Le G.,Jiangnan University | Shi Y.,Jiangnan University
Food and Chemical Toxicology | Year: 2014

Scope: Oxidized protein products (OPPs) can be easily found in meat and milk during processing and storage. Evidence supports that accumulation of endogenous OPPs plays a negative role in physiological metabolism. However, the impacts of dietary OPPs and the mechanisms have not been elucidated yet. The present study evaluated whether oral oxidized casein would destruct the antioxidant defense system and cause potential oxidized injury in mice liver and kidney. Methods and results: We performed oxidized casein (modified respectively by H2O2-Cu and HClO) feeding experiments using KM mice (20-22g). A 10-weeks feeding of oxidized casein as basal protein caused oxidative stress by increasing protein carbonylation (PC), advanced oxidation protein products (AOPPs), dityrosine (Dityr), lipid peroxidation and ROS levels in mice liver, kidney and blood (P<0.05). In mice liver and kidney, the mRNA expression of Nrf2, γ-GCS, HO-1, GPX-3, and GPX-4 up-regulated, the protein level of Nrf2 in nucleus increased. However, activities of anti-oxidant enzymes (CAT, SOD, and GPX) decreased (P<0.05). Moreover, histopathological examination displayed the formation of fibrous septa in mice liver and kidney after oxidized casein feeding. Conclusion: Oxidized casein impairs antioxidant defense system and induces hepatic and renal fibrosis. © 2013 .


Gao Z.-R.,Jiangnan University | Ji Z.-C.,Jiangnan University
Xi Tong Gong Cheng Yu Dian Zi Ji Shu/Systems Engineering and Electronics | Year: 2011

The problem of optimal guaranteed cost control for a class of switched singular systems with time-delay is considered. By means of Lyapunov function approaches and convex combination techniques, a sufficient condition for the existence of guaranteed cost sub-controllers is presented, which is in the form of linear matrix inequalities (LMIs), accordingly, both sub-controllers and switching strategy are designed. Furthermore, a convex optimization problem with LMIs constraints is formulated, the design of optimal guaranteed cost sub-controllers and the minimization of the upper boundary of closed-loop performance index are obtained by using the LMI toolbox in Matlab. Finally, a numerical example shows that the obtained results are effective.


Luo J.,Jiangnan University | Jiang S.,Jiangnan University | Wu Y.,Jiangnan University | Chen M.,Jiangnan University | Liu X.,Jiangnan University
Journal of Polymer Science, Part A: Polymer Chemistry | Year: 2012

A facile method of producing stable aqueous dispersion of graphene/polyaniline (PANI) composite is described, which involves the in situ polymerization of aniline on the surface of graphene with the aid of polystyrene sulfonic acid (PSS). The prepared aqueous graphene/PANI composite dispersion was very stable and no aggregation or precipitation was observed for several weeks. The excellent aqueous dispersibility and stability of the graphene/PANI composite is attributed to the cooperative interactions of π stacking interaction between PSS, PANI, and the graphene basal planes, and the electrostatic repulsions between negatively charged PSS bound on graphene/PANI composite. Fourier transform-infrared spectrometry (FTIR), ultraviolet-visible spectra (UV-vis), and Raman spectra confirmed the interaction of PANI and graphene in the composite, which effectively delocalize the electrons. In addition, the composite showed three orders of magnitude of conductivity increase compared with pure PANI. This new approach is simple, fast, and straightforward, representing a significant improvement in the processing of graphene/PANI composites. © 2012 Wiley Periodicals, Inc.


Chen Y.,University of Hohenheim | Chen Y.,Jiangnan University | Schwack W.,University of Hohenheim
Journal of Chromatography A | Year: 2014

In the European Union (EU), sulfonamides are among the most widely administrated groups of antibiotics in animal husbandry. Therefore, monitoring their residues in edible animal tissues plays an important role in the EU food safety framework. In this work, a simple and efficient method for the rapid screening of twelve prior sulfonamides frequently prescribed as veterinary drugs by high-performance thin-layer chromatography (HPTLC) was established. Sample extracts obtained with acetonitrile were tenfold concentrated and applied to HPTLC without any further cleanup. Following separation and fluram derivatization, sensitive and selective quantitation of the analytes can readily be accomplished with fluorescent densitometry. Limits of detection and quantitation were 15-40 and 35-70. μg/kg, respectively. Additionally, a confirmative detection by HPTLC-electrospray ionization mass spectrometry (HPTLC-ESI/MS) was optimized, offering straightforward identification of target zones. Therefore, the risk of potential false positive findings can efficiently be reduced. The method was validated to meet the enforced commission regulation (EU) No. 37/2010, regarding different matrix complexities (bovine milk, porcine liver and kidney). © 2014 Elsevier B.V.


Chen J.,Jiangnan University
Kongzhi yu Juece/Control and Decision | Year: 2015

This paper presents a novel identification method for a dual-rate sampled data system with preload nonlinearity. By using a switching function, the nonlinear system is turned into an identification model. Then a missing output identification model based recursive least-squares algorithm is derived to identify the parameters of the system by all the inputs and outputs. Compared with the polynomial transformation technique, this method can estimate the unknown parameters directly and can decrease the number of the unknown parameters. The simulation results show the effectiveness of the proposed algorithm. ©, 2015, Northeast University. All right reserved.


Ren S.-C.,Henan University of Technology | Qiao Q.-Q.,Henan University of Technology | Ding X.-L.,Jiangnan University
Czech Journal of Food Sciences | Year: 2013

Five flavones glycosides (i.e., 2''-O-a-l-rhamnosyl-6-C-3''deoxyglucosyl- 3'-methoxyluteolin, ax-5'-methane-3'- methoxymaysin, ax-4''-OH-3'-methoxymaysin, 6,4'-dihydroxy-3'-methoxyflavone-7-O-glucoside, and 7,4'-dihydroxy- 3'-methoxyflavone-2''-O-a-l-rhamnosyl-6-C-fucoside) were successfully isolated from corn silk and identified. We evaluated their antioxidant activity among in vitro assay systems. Most of the flavones glycosides showed a high antioxidant activity in a lecithin liposome system, as well as a strong scavenging activity against radicals such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radicals. The study indicated that the corn silk flavonoids, especially 6, 4'-dihydroxy- 3'-methoxyflavone-7-O-glucoside, may be beneficial natural food antioxidants. © 2011 Czech Academy of Agricultural Sciences.


Duan N.,Jiangnan University | Wu S.,Jiangnan University | Chen X.,Jiangnan University | Huang Y.,Jiangnan University | Wang Z.,Jiangnan University
Journal of Agricultural and Food Chemistry | Year: 2012

A whole-bacterium systemic evolution of ligands by exponential enrichment (SELEX) method was applied to a combinatorial library of FAM-labeled single-stranded DNA molecules to identify DNA aptamers demonstrating specific binding to Vibrio parahemolyticus. FAM-labeled aptamer sequences with high binding affinity to V. parahemolyticus were identified by flow cytometric analysis. Aptamer A3P, which showed a particularly high binding affinity in preliminary studies, was chosen for further characterization. This aptamer displayed a dissociation constant (Kd) of 16.88 ± 1.92 nM. Binding assays to assess the specificity of aptamer A3P showed a high binding affinity (76%) for V. parahemolyticus and a low apparent binding affinity (4%) for other bacteria. Whole-bacterium SELEX is a promising technique for the design of aptamer-based molecular probes for microbial pathogens that does not require the labor-intensive steps of isolating and purifying complex markers or targets. © 2012 American Chemical Society.


Tang X.-Q.,Jiangnan University | Zhu P.,Jiangnan University
IEEE Transactions on Fuzzy Systems | Year: 2013

In this paper, based on granular space, some hierarchical clustering problems and analysis for fuzzy proximity relation are developed by using rigorous mathematical descriptions, and four results are obtained. First, the granular representation of a fuzzy proximity relation is studied, an algorithm is obtained to compute the granular space (or hierarchical clustering structure) and the min-transitive closure derived from the same fuzzy proximity relation on a finite universe, and the consistent clustering properties are discussed. Second, a consistency index that is based on granular space is developed, and a global optimizing mathematical model is established to obtain the optimal clustering of a fuzzy proximity relation. Furthermore, for given two fuzzy proximity relations on different granulations of the same universe, a collaborative clustering method is studied by the intersection operation, and this method can provide a better hierarchical clustering structure. Finally, the theory for hierarchical clustering analysis of fuzzy proximity relations on granular space is established. For given two fuzzy proximity relations on the same universe, two sufficient conditions (Theorems 6.1 and 6.2) of isomorphism are given, and the necessary condition (Theorem 6.3) and the sufficient condition (Theorem 6.4) for -similarity are obtained. We also illustrate the effectiveness of these theories and methods with some practical examples. These results may help form a comprehensive theory and methodology for related potential application and will help us to obtain a deeper understanding of the essence of hierarchical clustering procedures. © 1993-2012 IEEE.


Zhang W.,Wuhan Polytechnic University | Xia W.,Jiangnan University
International Journal of Biological Macromolecules | Year: 2015

The effect of media milling on lipid-lowering and antioxidant activities of chitosan was studied in rats fed high-fat diets. Results showed that media-milled chitosan was more effective than chitosan in reducing body weight gain and liver fat accumulation of rats. Compared with chitosan, the reducing effects of media-milled chitosan on serum triacylglycerol (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were increased by 10.1, 7.5 and 10.2%, and liver TG and TC-reducing effects were increased by 16.2 and 14.6%, respectively. Rats fed media-milled chitosan showed decreased levels of free fatty acid (FFA) and thiobarbituric acid-reactive substances (TBARS) and increased superoxide dismutase (SOD) activity. The cholesterol and fat in feces of rats fed media-milled chitosan were higher than those of rats fed chitosan. These results suggested media milling enhanced the lipid-lowering and antioxidant activities of chitosan, and the reason might be partly due to its effect on strengthening the ability of chitosan in promoting fecal lipid excretions. © 2014 Elsevier B.V.


Ding F.,Jiangnan University | Zhang H.,Jiangnan University
IET Control Theory and Applications | Year: 2014

By constructing an objective function and using the gradient search, a gradient-based iteration is established for solving the coupled matrix equations AiXBi = Fi, i = 1, 2, ⋯, p. The authors prove that the gradient solution is convergent for any initial values. By analysing the spectral radius of the iterative matrix, the authors obtain an optimal convergence factor. An example is provided to illustrate the effectiveness of the proposed algorithm and to testify the conclusions established in this study. © 2014 The Institution of Engineering and Technology.


Liu L.,Jiangnan University | Zhang X.,Jiangnan University
Journal of Intelligent and Fuzzy Systems | Year: 2014

EQ-algebras were introduced by Novák and De Baets with the intent to develop an algebraic structure of truth values for fuzzy type theory. In this paper, the notions of implicative prefilters and positive implicative prefilters in EQ-algebras are introduced and several characterizations of them are obtained. The relationship between implicative prefilters and positive implicative prefilters is considered. It is proved that implicative prefilters and positive implicative prefilters are equivalent on good IEQ-algebras. The extension theorems of implicative prefilters and positive implicative prefilters are obtained. Furthermore, the quotient algebras induced by positive implicative filters are studied. © 2014 - IOS Press and the authors.


Mao Y.,Jiangnan University | Mao J.,Jiangnan University | Meng X.,Jiangnan University
Carbohydrate Polymers | Year: 2013

Response surface methodology was used to optimize the extraction parameters of EPS produced by Agaricus bisporus MJ-0811 in submerged culture. The optimal levels for ethanol concentration (85%, v/v), pH (8) and precipitation time (22 h) were determined, and EPS production was estimated at 2.71 g/L. The actual yield of EPS under these conditions was 2.69 g/L. In addition, the antioxidant activity of EPS was investigated by measuring its scavenging ability on superoxide radicals and hydroxyl radicals in vitro. Furthermore, the hypoglycemic activity of EPS was investigated by measuring its effects on body weights and blood glucose of diabetic mice. The study suggests that EPS has beneficial antioxidant activities (hydroxyl radical-scavenging activities, superoxide radical-scavenging activities) in vitro, anti-diabetic effect on alloxan induced diabetic mice. The EPS from A. bisporus may be a novel resource of natural antioxidants and anti-diabetic agents for use in the functional food or medicine. © 2012 Elsevier Ltd.


Zhang H.,University of Alabama in Huntsville | Kong X.-Z.,Jiangnan University
Computational Geometry: Theory and Applications | Year: 2016

Let G=(V,E) and G′=(V′,E′) be two graphs. A k-inverse-adjacency-preserving mapping ρ from G to G′ is a one-to-many and onto mapping from V to V′ satisfying the following: (1) Each vertex vεV in G is mapped to a non-empty subset ρ(v)⊂V′ in G′, the cardinality of ρ(v) is at most k; (2) if u≠v, then ρ(u)∩ρ(v)=θ; and (3) for any u′ερ(u) and v′ερ(v), if (u′,v′)εE′, then (u,v)εE. A vertex u′ in ρ(u) is called a virtual location for u. Let ρ be a k-inverse-adjacency-preserving-mapping (k-IAPM for short) from G to G′. Let δ be a greedy drawing of G′ into a metric space M. Consider a message from u to be delivered to v in G. Using the k-IAPM ρ from G to G′, intuitively, one can treat the message to be routed as if it were from one virtual location u′ for u to one virtual location v′ for v, except that all the virtual locations of a vertex u were identified with each other (which can be thought as instantaneously synchronized mirror sites for a particular website, for example). Then a routing path P′ can be computed from u′ to v′ while identifying all virtual locations for any vertex in G. Since ρ inversely preserves adjacency from G′ to G, such a routing path P′ corresponds to exactly one routing path P in G, which connects u to v. In this paper, we formalize the above intuition into a concept which we call k-greedy routing algorithm for a graph G with n vertices, where k refers to the maximum number of virtual locations any vertex of G can have. Using this concept, the result presented in [18] can be rephrased as a 3-greedy routing algorithm for 3-connected plane graphs, where the virtual coordinates used are from 1 to 2n-2. In this paper, we present a 2-greedy routing algorithm for 3-connected plane graphs, where each vertex uses at least one but at most two virtual locations numbered from 1 to 2n-1. For the special case of plane triangulations (in a plane triangulation, every face is a triangle, including the exterior face), the numbers used are further reduced to from 1 to ⌊5n+13⌊. Hence, there are at least ⌈n-13⌈ vertices that use only one virtual location. © 2015 Elsevier B. V. All rights reserved.


Xu C.,Jiangnan University | Long X.,Jiangnan University | Du J.,Jiangnan University | Fu S.,Jiangnan University
Carbohydrate Polymers | Year: 2013

There exists a misunderstanding on the TAED-activated peroxide system in the textile industry that H2O2 used in excess of the stoichiometric amount could produce an addition effect on bleaching of cotton under alkaline conditions. In this study, a critical reinvestigation was carried out on the TAED-activated peroxide system for bleaching of cotton. It was found that the TAED-activated peroxide system achieved its best performance under near-neutral pH conditions. No addition effect was observed when an excessive amount of H2O2 was used under alkaline conditions, which is probably due to the base-catalyzed bimolecular decomposition of peracetic acid and the nucleophilic attack by H2O2 on peracetic acid. NaHCO3 was shown to be a desired alkaline agent for maintaining near-neutral pH for the TAED-activated peroxide system. This study provides new insight into the application of the TAED-activated peroxide system for low-temperature bleaching of cotton under more environmentally benign conditions. © 2012 Elsevier Ltd. All rights reserved.


Li R.,Jiangnan University | Hu P.,Jiangnan University | Ren X.,Jiangnan University | Worley S.D.,Auburn University | Huang T.S.,Auburn University
Carbohydrate Polymers | Year: 2013

The inherent antimicrobial properties and biodegradability of chitosan make it an ideal candidate for antimicrobial materials. In this study, N-halamine precursor 3-glycidyl-5,5-dimethylhydantoin (GH) was synthesized and bonded onto chitosan by a ring opening reaction between chitosan and GH. The chitosan film modified with the N-halamine precursor could be rendered biocidal after exposure to a dilute household bleach solution. Syntheses routes, characterization data, and antimicrobial test results are presented. The chlorinated films with 2.60 × 1018 atoms/cm2 of active chlorine were challenged with Staphylococcus aureus (ATCC 6538) and Escherichia coli O157:H7 (ATCC 43895) and showed good efficacy against these two bacterial species with log reductions of 7.4 and 7.5 within 10 and 5 min of contact time, respectively. These films may serve as potential materials for food packaging and biomedical applications. © 2012 Elsevier Ltd. All rights reserved.


He S.,Anhui University | Liu F.,Jiangnan University
Journal of the Franklin Institute | Year: 2014

This paper studies the optimal finite-time passive control problem for a class of uncertain nonlinear Markovian jumping systems (MJSs). The Takagi and Sugeno (T-S) fuzzy model is employed to represent the nonlinear system with Markovian jump parameters and norm-bounded uncertainties. By selecting an appropriate Lyapunov-Krasovskii functional, it gives a sufficient condition for the existence of finite-time passive controller such that the uncertain nonlinear MJSs is stochastically finite-time bounded for all admissible uncertainties and satisfies the given passive control index in a finite time-interval. The sufficient condition on the existence of optimal finite-time fuzzy passive controller is formulated in the form of linear matrix inequalities and the designed algorithm is described as an optimization one. A numerical example is given at last to illustrate the effectiveness of the proposed design approach. © 2013 The Franklin Institute.


Zhang Y.,Jiangnan University | Zhang Y.,Shandong Agricultural University | Wang L.,Jiangnan University | Chen J.,Jiangnan University | Wu J.,Jiangnan University
Carbohydrate Polymers | Year: 2013

In the present study, cutinase-CBMCenA fusion protein was genetically modified in the carbohydratebinding module (CBM) binding sites, by site-directed mutagenesis, to enhance its activity toward polyethylene terephthalate (PET) fiber. The effects of tryptophan at particular positions of CBMCenA on the binding and hydrolysis of polyester substrate were investigated by replacing each of Trp14, Trp50 and Trp68 with leucine or tyrosine, respectively. All the mutants were expressed in Escherichia coli and purified to homogeneity. Enzyme characterization showed that the mutants displayed similar thermostability and pH stabilities in response to the native enzyme. Furthermore, W68L and W68Y, among all the mutants, exhibited significant improvement in binding and catalytic efficiency (1.4-1.5 fold) toward PET fiber when compared to that of the native enzyme. The enhanced binding and hydrolytic activity might be a result of creating new hydrogen bond or hydrophobic interaction between the enzyme and PET fiber. Crown Copyright © 2013 Published by Elsevier Ltd. All rights reserved.


Liu C.-L.,Jiangnan University | Liu F.,Jiangnan University
International Journal of Computers, Communications and Control | Year: 2010

Consensus problem of second-order multi-agent systems with velocity damping term in agent's dynamics is investigated. Based on frequency-domain anal-ysis, decentralized consensus condition, which depends on the input delays, is ob-tained for the system based on undirected and symmetric graph with heterogeneous input delays. For the system based on directed graph with both heterogeneous in-put delays and communication delays, decentralized consensus condition, which is dependent on the input delays but independent on the communication delays, is also obtained. Simulations illustrate the correctness of the results. © 2006-2010 by CCC Publications.


Liu G.,Jiangnan University | Hong Y.,Jiangnan University | Gu Z.,Jiangnan University
Carbohydrate Polymers | Year: 2013

This study aims to investigate the physicochemical and extended-release properties of amorphous debranched starch (ADBS) that is a linear short chain amylose derived from pullulanase enzymatic modification. The results show the completely amorphous ADBS was soluble in cold water, which developed into a disordered gel network at 25 °C. ADBS based tablets were able to extend drug release in different media. Drug release, which was accelerated by decreased pH and pancreatin, was barely affected by ionic strength. The kinetics of the drug release process that was dominated by Fickian diffusion was best fitted and interpreted by the Higuchi equation. The results indicate ADBS is an ideal hydrophilic excipient which extends the in vitro release of water-soluble drugs for 24 h. © 2013 Elsevier Ltd. All rights reserved.


Long X.,Jiangnan University | Xu C.,Jiangnan University | Du J.,Jiangnan University | Fu S.,Jiangnan University
Carbohydrate Polymers | Year: 2013

A low-temperature and near-neutral pH bleaching system was conceived for cotton by incorporating TAED, H2O2 and NaHCO3. The TAED/H2O2/NaHCO3 system was investigated and optimized for bleaching of cotton using a central composite design (CCD) combined with response surface methodology (RSM). CCD experimental data were fitted to create a response surface quadratic model (RSQM) describing the degree of whiteness of bleached cotton fabric. Analysis of variance for the RSQM revealed that temperature was the most significant variable, followed by [TAED] and time, while [NaHCO3] was insignificant. An effective system was conducted by adding 5.75 g L-1 TAED together with H2O 2 and NaHCO3 at a molar ratio of 1:2.4:2.8 and applied to bleaching of cotton at 70 °C for 40 min. Compared to a commercial bleaching method, the TAED/H2O2/NaHCO3 system provided cotton with comparable degree of whiteness, slightly inferior water absorbency and acceptable dyeability, but had competitive advantage in protecting cotton from severe chemical damage in bleaching. © 2013 Elsevier Ltd.


Chen J.,Jiangnan University
Journal of the Franklin Institute | Year: 2014

This paper presents three identification methods for dual-rate sampled systems. The first method combines the stochastic gradient algorithm with the polynomial transformation technique, which can estimate the parameters of the identification model. The second method is the finite impulse response model based stochastic gradient algorithm, which can indirectly estimate the parameters of the dual-rate systems by using all the inputs and the available outputs. The third method is the missing output estimation model based stochastic gradient algorithm with a forgetting factor, which can directly estimate the parameters of the dual-rate systems by using all the inputs and all the outputs (include the estimated outputs). An example is provided to verify the effectiveness of the proposed methods. © 2013 Published by The Franklin Institute. All rights reserved.


Gao Q.-H.,Northwest University, China | Wu C.-S.,Jiangnan University | Wang M.,Northwest University, China | Xu B.-N.,Northwest University, China | Du L.-J.,Northwest University, China
Journal of Agricultural and Food Chemistry | Year: 2012

This study investigated the changes in sugars, organic acids, α-tocopherol, β-carotene, phenolic profiles, total phenolic content (TPC), and antioxidant capacity of jujube fruits after four drying treatments (sun-, oven-, microwave- and freeze-drying). Sugar, organic acid, phenolic compounds, α-tocopherol, and β-carotene were qualitatived and quantitatived by high-performance liquid chromatography. The TPC and antioxidant activity of jujube samples were evaluated using the Folin-Ciocalteau method and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, respectively. Freeze-drying resulted in jujubes with higher antioxidant activity and was also a good choice for the preparation of β-carotene from jujubes for the food industry. Microwave-dried jujubes had a higher content of protocatechuic acid, catechin, and epicatechin and maintained the same antioxidant capacity with the freeze-dried jujubes. The combination of microwave- and freeze-drying may be an efficient alternative with shorter processing time and, consequently, less impact on the nutritional value of the jujube. © 2012 American Chemical Society.


Xu H.,Jiangnan University | Chai Y.,Jiangnan University | Zhang G.,Jiangnan University
Journal of Agricultural and Food Chemistry | Year: 2012

Oleic acid and glycerol are two different molecules with different plasticization mechanisms, and their effects on the mechanical properties of zein film were investigated. The mechanical parameters of tensile strength (TS) and elongation to break (E) were improved by either oleic acid or glycerol in a dose-dependent manner up to 20%. When a combination of the two plasticizers in different ratios was used to make zein films, the highest TS was observed at a ratio of 3:1 of oleic acid to glycerol, and a synergy between them was revealed through a statistical analysis. A significant decrease of glass transition temperature (Tg) and different microscopic patterns examined by scanning electronic microscopy (SEM) indicate the supramolecular structure was changed by the plasticizers. A combination of plasticizers with different plasticization mechanisms might be a better way to synergistically improve the mechanical properties of biopolymer-based films for practical applications. © 2012 American Chemical Society.


Wang C.,Jiangnan University | Mao H.,Jiangnan University | Fu S.,Jiangnan University
Industrial and Engineering Chemistry Research | Year: 2011

The high surface energy of TiO2 nanoparticulate leads to the aggregation of nanoparticles. The grafted modification of nanoparticles is necessary in order to improve its dispersibility in the organic solvent. The effect of grafted modification with silane coupling agent on dispersibility and lipophilicity of TiO2 nanoparticles was investigated. The factors of the modification were discussed, such as the amount of silane coupling agent, pH value, and reaction time. The average sizes of the TiO2 nanoparticles were 200-300 nm. The thermogravimetric analysis (TGA) curves indicated that the TiO2 nanoparticle surface had been grafted with the silane coupling agent. The zeta potential showed that modified TiO 2 could disperse well in the organic solvent. The lipophilic degree and contact angle suggested that the modified TiO2 had low surface energy and changed from a hydrophilic nature to a lipophilic nature. © 2011 American Chemical Society.


Wu Q.,Jiangnan University | Xu Y.,Jiangnan University
Journal of Agricultural and Food Chemistry | Year: 2012

Although Maotai flavor liquor is exclusive due to its soy sauce flavor, knowledge of its key compound and production mechanism is still scarce until now. To gain insight into the production mechanism of soy sauce flavor, a soy sauce flavor producing strain with high efficiency and heat-resistant capability was obtained, and the metabolic mechanism of the strain was investigated with the technique of microarray profiling. Because high temperature was a key factor for soy sauce flavor production, the global gene expression of this heat-resistant strain fermented at 55 °C was analyzed. Except for the responsive increase of heat shock proteins, which maintained cell survival during heat stress, biosynthesis of cysteine was also up-regulated. In addition, some metabolites were significantly increased when cysteine was added to the fermentation medium, such as 2,3-butanediol, 3-hydroxy-2-butanone, and tetramethylpyrazine, which were important flavor compounds in soy sauce flavor liquor and might be related with soy sauce flavor. The results indicated that cysteine might play an important role in the formation of soy sauce flavor compound, and it might act as an indirect precursor or stimulator of soy sauce flavor formation. This was the first use of the microarray profiling tool to investigate the fermentative strains for Chinese traditional liquor, which would allow a deeper insight into the mechanism of the formation of soy sauce flavor compound. © 2012 American Chemical Society.


Zhou T.Q.,Jiangnan University
Applied Mechanics and Materials | Year: 2013

Horizontal freezing induces ground frost heave and ground displacement happens. Excessive uneven displacement or deformation may be dangerous to the surrounding building. The stratum that a Nanjing subway tunnel will traverse through is the soft and silt clay. The artificial freezing construction method is proposed to help the tunnel to traverse through the worse stratum. To control the ground building deformation and displacement due to the ground frost heave, the frost heave effect for the artificial freezing construction should be predicted before the construction process. The stochastic media theory is proposed to predict the frost heave effect due to the artificial freezing construction in the paper. The basic analysis procedure for analyzing frost heave effect is introduced and ground deformations such as ground vertical displacement, ground curvature are analyzed. The analysis result shows that the ground deformation due to the frost heaves is in the limit of ground displacement control requirement. © (2013) Trans Tech Publications, Switzerland.


Wen J.-W.,Jiangnan University | Liu F.,Jiangnan University | Kiong Nguang S.,University of Auckland
Journal of the Franklin Institute | Year: 2012

This paper presents a sampled-data predictive control strategy for a class of uncertain continuous-time Markovian jump linear system (MJLS) with time-varying delay. The system under consideration covers MJLS with completely known jump rates and arbitrary switched linear system. The predictive formulation utilizes both off-line and on-line optimization paradigms. The feasibility of the control scheme and the stability of the closed-loop system are investigated by introducing a modified stochastic invariant ellipsoid. The conditions for the existence of a stabilizing optimal controller for the underlying system are obtained via the semi-definite programming (SDP). A numerical example is given to verify efficiency and potential of the developed approach. © 2011 The Franklin Institute. Published by Elsevier Ltd. All rights reserved.


Dong W.,Jiangnan University | Ruan X.,Jiangnan University | Ni Z.,Jiangnan University | Chen M.,Jiangnan University
Polymer Degradation and Stability | Year: 2013

The influence of soy protein isolate (SPI) and its mixture with calcium stearate (CaSt2), and zinc stearate (ZnSt2) with various ratios on the processing thermal stability of poly (vinyl chloride) (PVC) was investigated by measuring dehydrochlorination rate of PVC (DHC), visual color comparison, and thermogravimetric analysis (TGA). The results suggested that SPI acted as a long-term thermal stabilizer like CaSt2 to absorb HCl released from PVC, which is due to the primary amine (-NH2) and polyols containing hydroxyl groups in SPI. Moreover, SPI improved the stabilization effect of the traditional CaSt2/ZnSt2 stabilizer, and the combination of SPI and CaSt2/ZnSt2 at certain ratios exhibited desirable stabilization efficiency in prolonging the discoloration time. © 2012 Elsevier Ltd. All rights reserved.


He S.,Anhui University | Liu F.,Jiangnan University
IEEE Transactions on Fuzzy Systems | Year: 2012

This paper studies the finite-time H ∞ control problem for time-delay nonlinear jump systems via dynamic observer-based state feedback by the fuzzy Lyapunov-Krasovskii functional approach. The Takagi-Sugeno (T-S) fuzzy model is first employed to represent the presented nonlinear Markov jump systems (MJSs) with time delays. Based on the selected Lyapunov-Krasovskii functional, the observer-based state feedback controller is constructed to derive a sufficient condition such that the closed-loop fuzzy MJSs is finite-time bounded and satisfies a prescribed level of H ∞ disturbance attenuation in a finite time interval. Then, in terms of linear matrix inequality (LMIs) techniques, the sufficient condition on the existence of the finite-time H ∞ fuzzy observer-based controller is presented and proved. The controller and observer can be obtained directly by using the existing LMIs optimization techniques. Finally, a numerical example is given to illustrate the effectiveness of the proposed design approach. © 1993-2012 IEEE.


Proteomic analysis was applied to investigate the mechanism of the stimulatory effect of Tween 80 on the mycelial growth and exopolysaccharide production by an edible mushroom Pleurotus tuber-regium. 32 differentially expressed proteins were identified by one-dimension gel electrophoresis. Combined with our previous findings, the up-regulation of heat shock proteins might help to maintain cellular viability under environmental stress. The up-regulation of ATP:citrate lyase isoform 2 could suppress the activity of tricarboxylic acid cycle and, consequently, stimulate exopolysaccharide production. The present results provide important insight to the mechanism by which stimulatory agents (Tween 80) can increase the production of useful fungal metabolites and also fill the gap of our knowledge on the under-developed mushroom proteomics.


Zhang B.-B.,Jiangnan University | Lu L.-P.,Jiangnan University | Xu G.-R.,Jiangnan University
Journal of Biotechnology | Year: 2015

The underlying mechanisms by which solid-state fermentation (SSF) was more advantageous over submerged fermentation (SmF) for converting high concentration of glycerol into Monacolin K by Monascus purpureus were investigated innovatively. First, the established kinetic models and kinetic parameters showed that the cell growth, Monacolin K formation and glycerol consumption in SSF were more rapid than those in SmF. Secondly, the comparison of fatty acid composition of mycelial cells indicated a better fluidity and permeability of the cell membrane in SSF than that of SmF, which was also consistent with the difference in the ratio of extracellular/intracellular Monacolin K between the two systems. Thirdly, the phenomenon of glycerol concentration gradient was verified in SSF, which could well explain the resistance effect to high concentration of glycerol in SSF. These new findings provide some important insights to the elucidation of the advantages of SSF for the synthesis of fungal secondary metabolites. © 2015 Elsevier B.V.


Wang J.,Jiangnan University | Zheng P.,Jiangnan University
Journal of Industrial Microbiology and Biotechnology | Year: 2015

Muconic acid (MA) is a promising bulk chemical due to its extensive industrial applications in the production of adipic acid and other valuable, biodegradable intermediates. MA is heretofore mainly produced from petrochemicals by organic reactions which are not environmentally friendly or renewable. Biological production processes provide a promising alternative for MA production. We designed an artificial pathway in Escherichia coli for the biosynthesis of MA using the catechol group of 2,3-dihydroxybenzoate, an intermediate in the enterobactin biosynthesis pathway. This approach consists of two heterologous microbial enzymes, including 2,3-dihydroxybenzoate decarboxylase and catechol 1,2-dioxygenase. The metabolic flow of carbon into the heterologous pathway was optimized by increasing the flux from chorismate through the enterobactin biosynthesis pathway and by regulating the shikimate pathway. Metabolic optimization enabled a concentration of 605.18 mg/L of MA from glucose in a shaking flask culture, a value nearly 484-fold higher than that of the initial recombinant strain. The results indicated that the production of MA from this pathway has the potential for further improvement. © 2015, Society for Industrial Microbiology and Biotechnology.


Lu X.-Y.,Jiangnan University
Energy Education Science and Technology Part A: Energy Science and Research | Year: 2014

Methods the virtual prototype simulation for this article, in the research phase of product design, were observed, recorded and compared to the construction of steel structure of tower crane and tower body, no limit element analysis, combined with the software and research, supplement each other the tower stress dangerous location, then fatigue analysis, fatigue tower personal data analysis. At the same time, provides certain reference for the other tower crane parts design. See through the dynamic simulation which can load position, the tower was affected by lifting acceleration and deceleration influence as well as acceleration and deceleration and the emergence of a transient vibration, the tower body string truss welding position to produce the initial crack, have very big effect. By comparison the lifting (rotary) velocity is small. The hoisting (rotation) motion acceleration, dynamic system as well as the impact of wind load can make the machine has been in a slight vibration, if unreasonable design, power system and the inherent frequency, the vibration is more intense, the driver long time in such an environment, will produce the body the discomfort, a long time will cause harm to the safety of construction, it should consider the as design. © Sila Science. All Rights Reserved.


Fang Y.-Q.,Jiangnan University
Chinese Journal of Biologicals | Year: 2015

Objective: To improve the thermo-stability and catalytic activity of thermo-sensitive nitrile hydratase (NHase, EC 4. 2. 1. 84) of Psedomonas putida NRRL-18668 by molecular modification using computer-aided semi-rational design. Methods: Homologous target fragments were screened by site-targeted amino acid recombination (STAR) software and molecular dynamic modeling using the NHases from Pseudonocardia thermophila JCM3095 and Comamonas testosterone 5-MGAM-4D as templates respectively. Seven chimeric NHases were generated by homologous fragment swapping on the corresponding fragment of NHase from Psedomonas putida NRRL-18668, and determined for thermo-stability and activity. The secondary structures of wild-type NHase and chimeric NHase 3AB were analyzed by circular dicroism (CD) spectrometry. Results: Compared with that of wild-type NHase, the thermo-stabilities of seven chimeric NHases (1A, 2B, 2C, 2BC, 3AB, 3AC and 3ABC) after treatment at 50 °C for 10 min increased by 1. 5 ∼3. 5 folds. The thermo-stability and activity of NHase 3AB increased by 3. 5 and 1. 4 folds respectively. The α-helixe contents in secondary structures of wild-type NHase and chimeric NHase 3AB were (34. 56 ± 3. 21)% and (36. 88 ± 1. 41)%, while the (3-sheet contents were (19. 78 ± 2. 14)% and (18. 69 ± 1. 74)%, respectively. Conclusion: The thermo-sensitive NHase was rationally engineered to sets of thermo-stable chimeric enzyme using homologous fragment swapping by substituting themo-sensitive domains with homologous thermo-stable domain, of which the specific activity increased without alteration in secondary structure.


Wu S.,Jiangnan University | Duan N.,Jiangnan University | Shi Z.,Jiangnan University | Fang C.,Jiangnan University | Wang Z.,Jiangnan University
Analytical Chemistry | Year: 2014

A highly sensitive and specific multiplex method for the simultaneous detection of three pathogenic bacteria was fabricated using multicolor upconversion nanoparticles (UCNPs) as luminescence labels coupled with aptamers as the molecular recognition elements. Multicolor UCNPs were synthesized via doping with various rare-earth ions to obtain well-separated emission peaks. The aptamer sequences were selected using the systematic evolution of ligands by exponential enrichment (SELEX) strategy for Staphylococcus aureus, Vibrio parahemolyticus, and Salmonella typhimurium. When applied in this method, aptamers can be used for the specific recognition of the bacteria from complex mixtures, including those found in real food matrixes. Aptamers and multicolor UCNPs were employed to selectively capture and simultaneously quantify the three target bacteria on the basis of the independent peaks. Under optimal conditions, the correlation between the concentration of three bacteria and the luminescence signal was found to be linear from 50-106 cfu mL -1. Improved by the magnetic separation and concentration effect of Fe3O4 magnetic nanoparticles, the limits of detection of the developed method were found to be 25, 10, and 15 cfu mL-1 for S. aureus, V. parahemolyticus, and S. typhimurium, respectively. The capability of the bioassay in real food samples was also investigated, and the results were consistent with experimental results obtained from plate-counting methods. This proposed method for the detection of various pathogenic bacteria based on multicolor UCNPs has great potential in the application of food safety and multiplex nanosensors. © 2014 American Chemical Society.


Chen J.,Jiangnan University
Journal of Chinese Institute of Food Science and Technology | Year: 2013

With the increase of economic level and the improvement of the health concerns, people are increasingly concerned about the safety, nutrition and function of the food. Therefore, functional nutritional chemicals are now the trend of the food industry. Although the chemical functional nutrition has a long history in China, and China has a wealth of herbs and other functional nutritional health products featured resources, our research in this area is still relatively backward. The current review systematically reviewed the concept, characteristics and market of the functional nutritional chemicals, consideration the development of the chemicals in last decades. We hope that the introduction of the status quo of functional nutritional chemicals could provide a reference for the future development of the industry.


Wang B.-X.,Jiangnan University
Plasmonics | Year: 2016

Multi-band or broadband perfect metamaterial absorbers, based on coplanar super-unit structure or multiple vertically stacked layers, have received intense attention because of their potential for practical applications. The resonance mechanism of them usually only utilizes the overlapping of the fundamental resonance of the different-sized patterns, and neglects the high-order resonance of the structure, and thus making the proposed structures quite troublesome to be fabricated and the mechanism of the current demonstrated absorbers lack of novelty. In this paper, a simple design of dual-band terahertz absorber consisted of only a traditional square metallic patch and a dielectric layer on top of a continuous ground plane is presented. Simulation results show that the single resonant structure has two resonance absorption peaks, which are both average over 99.5 %. The mechanism of the dual-band absorber is due to the overlapping of the fundamental mode and three-order response of the patterned structure, which is totally different from previous reports that only combining the fundamental resonances of the different-shaped complex structures to obtain the dual-band response. Furthermore, the proposed single-patterned structure can be used to extend the number of the absorption peaks (for example, triple-band absorber) by combining one more resonance (the five-order response). The proposed absorbers with the simple structure design have potential applications in many areas, such as detection, sensing, and selective thermal emitters. © 2016 Springer Science+Business Media New York


Liu T.,Jiangnan University | Zou W.,Jiangnan University | Liu L.,Jiangnan University | Chen J.,Jiangnan University
Biotechnology for Biofuels | Year: 2012

Background: As one of the best xylose utilization microorganisms, Scheffersomyces stipitis exhibits great potential for the efficient lignocellulosic biomass fermentation. Therefore, a comprehensive understanding of its unique physiological and metabolic characteristics is required to further improve its performance on cellulosic ethanol production. Results: A constraint-based genome-scale metabolic model for S. stipitis CBS 6054 was developed on the basis of its genomic, transcriptomic and literature information. The model iTL885 consists of 885 genes, 870 metabolites, and 1240 reactions. During the reconstruction process, 36 putative sugar transporters were reannotated and the metabolisms of 7 sugars were illuminated. Essentiality study was conducted to predict essential genes on different growth media. Key factors affecting cell growth and ethanol formation were investigated by the use of constraint-based analysis. Furthermore, the uptake systems and metabolic routes of xylose were elucidated, and the optimization strategies for the overproduction of ethanol were proposed from both genetic and environmental perspectives. Conclusions: Systems biology modelling has proven to be a powerful tool for targeting metabolic changes. Thus, this systematic investigation of the metabolism of S. stipitis could be used as a starting point for future experiment designs aimed at identifying the metabolic bottlenecks of this important yeast. © 2012 Liu et al.; licensee BioMed Central Ltd.


Wang X.,Jiangnan University | Ding F.,Jiangnan University
Journal of the Franklin Institute | Year: 2014

Two auxiliary model based recursive identification algorithms, a generalized extended stochastic gradient algorithm and a recursive generalized extended least squares algorithm, are developed for multivariable Box-Jenkins systems. The basic idea is to use the auxiliary models to estimate the unknown noise-free outputs of the system and to replace the unmeasurable terms in the information vectors with their estimates. We prove that the estimation errors given by the proposed algorithms converge to zero under the persistent excitation condition. Finally, an example is provided to show the effectiveness of the proposed algorithms. © 2014 The Franklin Institute. Published by Elsevier Ltd. All rights reserved.


Wang Y.X.,Jiangnan University | Cao J.,Nanjing University | Xiong S.J.,Nanjing University
European Physical Journal B | Year: 2012

We investigate theoretically Zitterbewegung behavior of Dirac oscillator after the excitation by a short laser pulse. With the help of the time-dependent perturbation theory, the dipole moments and the corresponding electric fields are calculated. We simulate the asymptotic limiting cases in relativistic and non-relativistic regimes with the quantum optic experiment and the results indicate that the ZB motion and the electromagnetic radiation tend to vanish in both limits. Further we analyze the Fourier frequency spectra of ZB motion. © EDP Sciences, Societá Italiana di Fisica, Springer-Verlag 2012.


Zhang P.,Tianjin University | Zhang P.,Jiangnan University | Ma X.,Tianjin University
Chemical Engineering Journal | Year: 2010

Direct synthesis of diethyl carbonate (DEC) by oxidative carbonylation of ethanol offers a prospective " green chemistry" strategy to eliminate the phosgene used in the traditional preparation processes. PdCl2/Cu-HMS catalyst has been investigated, which demonstrated excellent selectivity to DEC by oxidative carbonylation of ethanol in the gas-phase reaction. The Si/Cu molar ratio of mesoporous Cu-HMS supports showed a remarkable effect on catalytic activities. An optimized Si/Cu molar ratio existed for catalytic performance, which was about 50/1. From XPS, ICP, XRD, nitrogen physisorption and IR characterization and analysis, it could be concluded that copper species incorporated into HMS frame and was highly dispersed in the frame of silica. The catalytic performances of PdCl2/Cu-HMS were related with both Cu content in the Cu-HMS and the order degree of mesoporous structure for Cu-HMS. © 2010 Elsevier B.V.


Fan M.,Jiangnan University | Yang J.,Jiangnan University | Jiang P.,Jiangnan University | Zhang P.,Jiangnan University | Li S.,Jiangnan University
RSC Advances | Year: 2013

Biodiesel produced by transesterification of vegetable oils or animal fats is a potential alternative fuel to diesel, given the limited resources of fossil fuel and its environmental concerns. Three novel dicationic basic ionic liquids (DBILs) have been prepared for synthesis of biodiesel from soybean oil. Among them, 1, 2-bis (3-methylimidazolium-1-yl) ethylene imidazolide performed best and the yield of biodiesel reached 99.6%, which was related to the basicity and miscibility properties of the IL. © 2013 The Royal Society of Chemistry.


He D.,Jiangnan University | Zheng Y.,CAS Kunming Institute of Zoology | Tam S.,Chinese University of Hong Kong
Life Sciences | Year: 2012

Aims: Trichosanthin (TCS) is a type I ribosome-inactivating protein. We have previously shown that TCS induces a more potent apoptosis in infected cells over uninfected cells, but the mechanism underlying it is unclear. In this study, we explored the anti-HSV-1 mechanism of TCS through the nuclear factor-κB (NF-κB) and p53 pathways in human epithelial carcinoma (HEp-2) cells with wild type p53. Main methods: The western blot, electrophoretic mobility shift assay, chromatin immunoprecipitation assay, enzyme-linked immunosorbent assay and cytokinesis-block micronucleus were applied in this study. Key findings: It was shown that TCS inhibited the HSV-1-induced NF-κB activation. Meanwhile, in HSV-1 infected cells, TCS treatment activated significantly more p53 and BAX, with no DNA damage and less S phase arrest compared with uninfected cells. The activation of BAX in infected cells correlated with the cell death signaling of p53. Significance: Taken together, these results suggest that the anti-HSV-1 effect of TCS is related to its suppression of NF-κB activation and regulation of p53-dependent cell death in infected cells. © 2012 Elsevier Inc.


Luo J.,Jiangnan University | Chen Y.,Jiangnan University | Ma Q.,Jiangnan University | Liu R.,Jiangnan University | Liu X.,Jiangnan University
RSC Advances | Year: 2013

A new class of graphene/polyaniline multilayer film was constructed by layer-by-layer self-assembly, using poly(sodium 4-styrenesulfonate) stabilized graphene sheets (PSS-GS) and polyaniline (PANI) as building blocks. Poly(sodium 4-styrenesulfonate) (PSS) was used not only to stabilize the graphene sheets, but also facilitate the growth of the PSS-GS/PANI multilayer films via electrostatic interactions between PSS-GS and PANI. Furthermore, PSS can also act as a dopant for PANI during redox reactions. Ultraviolet-visible absorption spectrum (UV-vis), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscopy (SEM) results demonstrate the successful fabrication of the PSS-GS/PANI hybrid multilayer films. Electrochemical experiments show that PSS-GS inside the multilayer film can dope PANI effectively and the multilayer film still presents a redox activity at neutral pH. In addition, the obtained nanostructured PSS-GS/PANI multilayer film is very stable and shows high electrocatalytic ability toward H 2O2, which makes it an ideal substrate for H 2O2 detection and offers great promise for sensing. This journal is © The Royal Society of Chemistry.


Zhang H.,Jiangnan University | Jin Z.,Jiangnan University
Carbohydrate Polymers | Year: 2011

In this paper, high resistant starch content product was prepared by hydrolyzing of maize starch with pullulanase. The optimal hydrolyzing conditions were investigated and the optimum conditions were as follows: time, 32 h; pH, 5.0; temperature, 46 °C; amount of pullulanase, 12 ASPU/g. The product of resistant starch was obtained by pressure-cooking the resulting hydrolysate in an autoclave at 121 °C for 1 h, cooling at room temperature, storing at 4 °C overnight, autoclaving/cooling for 2 repetition cycle, drying an oven (105 °C) and finally grounding into fine particles (<150 μm). The content of resistant starch in the product was 44.7% (w/w). Results of experiments indicated that this was a promising way of preparation of product of high content of resistant starch. © 2010 Elsevier Ltd. All rights reserved.


Li J.,Jiangnan University | Fan L.,Jiangnan University | Ding S.,Jiangnan University
Carbohydrate Polymers | Year: 2011

A novel water-soluble polysaccharide (ZSP3c) was isolated from Zizyphus jujuba cv. Jinsixiaozao and its structure was investigated. ZSP3c was obtained by extraction with hot water followed by purification using DEAE-SepharoseCL-6B anion-exchange, SepharoseCL-6B and Sephadex G-200 column chromatography. It was eluted as a single symmetrical narrow peak on high-performance gel-permeation chromatography (HPGPC) and the average molecular weight (Mw) was estimated to be 1.4 × 105 Da. The ZSP3c was composed of l-rhamnose, d-arabinose and d-galactose in a molar ratio of 1:2:8. The structural features of ZSP3c were investigated by partial hydrolysis with acid, methylation analysis and NMR spectroscopy analysis. The results revealed that the main backbone chain of ZSP3c was (1 → 4)-d-galacturonopyranosyl residues interspersed with (1 → 2)-l-rhamnopyranosyl residues and (1 → 2,4)-l- rhamnopyranosyl residues. The neutral side chains of arabinofuranosyl residues and galactopyranosyl residues were attached to the backbone at the O-4 position of rhamnopyranosyl residues. © 2010 Elsevier Ltd. All rights reserved.


Feng Y.,Jiangnan University | Xia W.,Jiangnan University
Carbohydrate Polymers | Year: 2011

A novel water-soluble chitosan derivative, O-fumaryl-chitosan (OFCS), was prepared by using the selective partial acylation of chitosan and fumaric acid in the presence of H 2SO 4. The chemical structure and physical properties of OFCS were characterized by FTIR, 1H NMR, 13C NMR, and TG techniques. Our results showed that the degree of substitution (DS) for the chitosan derivatives was from 0.07 to 0.48 and the prepared derivatives had good solubility over a wide pH range, for example, the solubility of OFCS increased from 10.5 to 52.6 mg/ml as the DS increased. The antibacterial activity of chitosan and OFCS was investigated against Escherichia coli and Staphylococcus aureus. The results indicated that the antibacterial activity of OFCS was much stronger than that of chitosan and it increased with increasing DS increasing. These findings suggest that the OFCS with a DS of 0.48 is preferable for use as a food preservative. © 2010 Elsevier Ltd. All rights reserved.


Zhou Z.,Jiangnan University | Wang J.,Jiangnan University
Chinese Control Conference, CCC | Year: 2014

Due to the existing Apriori association rules data mining algorithms require to scan the database many times and generate a large numbers of candidate sets, which produce giant I/O expense issues, result in low data mining computational efficiency. Matrix algorithms can improve the efficiency in computing frequency 2-itemset, but not delete non-frequency item set before calculation, not effectively improved efficiency. A matrix-based and sorting index association rules algorithm is proposed. Firstly, delete the unwanted affairs and items, the frequent binomial set obtained by matrix multiplying and search table, combined with sorting index derived the rest of the frequency k-itemsets. Compared with Apriori algorithm and matrix algorithm, the proposed algorithm scan database only once, which can directly find the frequency k-itemsets, especially when frequent item sets are higher or need to have a date mining update, the algorithm has higher efficiency and feasibility. Experiment shows that proposed matrix sorting index algorithm greatly improved the data mining efficiency and scalability. © 2014 TCCT, CAA.


Zhang H.,Jiangnan University | Song T.,Jiangnan University
Electrochimica Acta | Year: 2013

The F-doped layered cathode materials Li1.05(Ni 0.5Mn0.5)0.95O2-xFx(0 ≤ x ≤ 0.1) with ball-like shape were successfully synthesized by carbonate co-precipitation method, followed by a high temperature calcination process. The effects of fluorine content on the crystal structure, morphology and electrochemical performance were extensively investigated. The XRD results reveal that all the materials synthesized have typical hexagonal structure without impurity. Fluorine-doped materials could have improved cycling performance compared with Li1.05(Ni0.5Mn 0.5)0.95O2, and Li1.05(Ni 0.5Mn0.5)0.95O1.95F0.05 shows the best electrochemical property. In addition, 3 wt% FePO 4-coated Li1.05(Ni0.5Mn0.5) 0.95O1.95F0.05 has significantly improved rate capability and better thermal stability compared with pristine sample. Electrochemical impedance spectroscopy (EIS) measurements reveal that both fluorine substitution and FePO4 coating could suppress the increase of charge transfer resistance with cycling. Overall, the combination of fluorine doping and FePO4 coating was effective in improving both cycling performance and rate capability of Li1.05(Ni0.5Mn 0.5)0.95O2. © 2013 Elsevier Ltd.


Liu Y.,Jiangnan University
Journal of Information and Computational Science | Year: 2011

A novel hybrid algorithm based Radial Basis Function (RBF) neural network is proposed for network anomaly detection in this paper. The Quantum-behaved Particle Swarm Optimization, which outperforms other optimization algorithm considerably on its simple architecture and fast convergence, has previously applied to solve optimum problem. However, the QPSO also has its own shortcomings. So, a hybrid algorithm in training RBF neural network was proposed. This new evolutionary algorithm, which is based on a hybrid of Quantum-Behaved Particle Swarm Optimization (QPSO) and Gradient Descent (GD) algorithm, is employed to train RBFNN. Experimental result on KDD99 intrusion detection datasets shows that this RBFNN using the novel hybrid algorithm has high detection rate while maintaining a low false positive rate. Copyright © 2011 Binary Information Press.


Ye C.,Jiangnan University | Zou W.,Jiangnan University | Xu N.,Jiangnan University | Liu L.,Jiangnan University
Journal of Biotechnology | Year: 2014

An artificial microbial ecosystem (AME) consisting of Ketogulonicigenium vulgare and Bacillus megaterium is currently used in a two-step fermentation process for vitamin C production. In order to obtain a comprehensive understanding of the metabolic interactions between the two bacteria, a two-species stoichiometric metabolic model ( iWZ-KV-663-BM-1055) consisting of 1718 genes, 1573 metabolites, and 1891 reactions (excluding exchange reactions) was constructed based on separate genome-scale metabolic models (GSMMs) of K. vulgare and B. megaterium. These two compartments (k and b) of iWZ-KV-663-BM-1055 shared 453 reactions and 548 metabolites. Compartment b was richer in subsystems than compartment k. In minimal media with glucose (MG), metabolite exchange between compartments was assessed by constraint-based analysis. Compartment b secreted essential amino acids, nucleic acids, vitamins and cofactors important for K. vulgare growth and biosynthesis of 2-keto-. l-gulonic acid (2-KLG). Further research showed that when co-cultured with B. megaterium in l-sorbose-CSLP medium, the growth rate of K. vulgare and 2-KLG production were increased by 111.9% and 29.42%, respectively, under the constraints employed. Our study demonstrated that GSMMs and constraint-based methods can be used to decode the physiological features and inter-species interactions of AMEs used in industrial biotechnology, which will be of benefit for improving regulation and refinement in future industrial processes. © 2014 Elsevier B.V.


Wang X.,Jiangnan University | Chen Y.,Jiangnan University | Yuan B.,Jiangnan University | Li X.,Jiangnan University | Ren Y.,Jiangnan University
Bioresource Technology | Year: 2014

Sludge retention time (SRT) is a feasible method to alleviate the salt accumulation in the osmotic membrane bioreactor (OMBR) by discharging the waste activated sludge. In this study, effects of SRT on sludge characteristics and membrane fouling were investigated using a submerged OMBR under two SRTs of 10 and 15d. The results showed that the lower SRT was helpful for alleviating the salt accumulation and flux decline. Besides that, the removal of NH3-N was significantly influenced by SRT. SRT also had a strong effect on soluble microbial products (SMP) and microbial activity due to the variation of salinity. Microbial diversity analysis indicated that the high salinity environment in the OMBR significantly affected the microbial communities. The flux decline in the OMBR was mainly attributed to the reduced driving force resulting from the salt accumulation, and the reversible fouling was the dominant forward osmosis (FO) membrane fouling in the OMBR. © 2014 Elsevier Ltd.


Hu J.,Jiangnan University | Li Y.,Jiangnan University | Zhang H.,Jiangnan University | Tan Y.,Jiangnan University | Wang X.,Jiangnan University
Plasmid | Year: 2014

Corynebacterium glutamicum is an important microorganism for production of amino acids in industrial fermentation. Suitable vectors are needed for metabolic engineering in C. glutamicum. Most available vectors used in C. glutamicum carry antibiotic resistant genes as a genetic labeling for rapid identification of recombinant strains, and antibiotics have to be added to maintain the vector when growing the cells. These vectors, though excellent for laboratory use, are not preferable choices for industry-scale fermentation. In this work, we developed a novel expression system for use in C. glutamicum, which do not require antibiotics when used for industrial fermentation. This system includes two vectors: the shuttle vector pJYW-4 for expression of genes and the vector pJYW-6 for deletion of the essential gene alr in C. glutamicum. The vector pJYW-4 contains a large multiple cloning site for cloning multiple genes and two selective markers: one is the kanamycin-resistant gene kan and the other is an essential gene alr. The selective marker kan facilitates molecular manipulation or fermentations in the laboratory, and the selection marker alr is good for use in industry-scale fermentation, allowing in vivo maintenance of the expression vector through auxotrophic complementation; therefore, the two selection markers in pJYW-4 make it useful for both laboratory research and industrial fermentation, and convenient to transfer valuable laboratory-developed strains into industrial production. This newly-constructed expression system was successfully used to increase l-valine production in C. glutamicum ATCC 14067, indicating its potential on developing amino acid-producing C. glutamicum strains. © 2014 Elsevier Inc.


Wang Q.,Jiangnan University | Hauser P.J.,North Carolina State University
Carbohydrate Polymers | Year: 2010

A new approach for UV protection of cotton fabrics based on electrostatic self-assembly (ESA) technique was studied in this paper. Three fluorescent brightening agents (FBAs) and poly(diallyldimethylammonium chloride) (PDDA) were stepwisely fabricated on cationized cotton fabrics through direct layer-by-layer (LbL) deposition technique. Dyeing of the assembled cotton fabrics with anionic dyes shows regular and identifiable "odd-even" changes in color depth (K/S value), indicating the variation of surface polarities of the cotton substrates due to the alternate deposition of FBA and PDDA on them. The stepwise increases in UPF of treated cotton fabrics further revealed the growth of these LbL deposition multilayers. The assembled cotton fabrics could obtain excellent UV protection ratings of UPF > 40 after several bilayers of FBA/PDDA were fabricated, depending on the type of FBAs. Excellent durability to washing of the (FBA/PDDA)n multilayers was obtained, which indicates good adhesion between the multilayer coatings and the cotton surfaces. © 2010 Elsevier Ltd. All rights reserved.


Zhang H.,Jiangnan University | Zhang H.,Jiangsu Animal Husbandry and Veterinary College | Jin Z.,Jiangnan University
Carbohydrate Polymers | Year: 2011

We prepared resistant starch (RS) from maize starch using a method combining α-amylase and pullulanase. We optimized reaction conditions for α-amylase, including temperature (T), pH, reaction time (t), and amount of α-amylase. The highest formation of RS (58.87%) was obtained under the following conditions: temperature, 90°C; pH, 5.5; time, 15 min; and amount of α-amylase, 4 μ/g. Scanning electron microscopy and differential scanning calorimetric analyses showed that maize starch underwent pasting treatment with α-amylase contained shorter amylase chains, and decreased steric hindrance among molecules compared with native maize starch. It is advantageous for amylopectin to be debranched by pullulanase, and the short amylose chains released from amylopectin can form double helices. Compared with native maize starch, maize RS showed increased crystallinity, and a larger, more compact laminiplantation structure. The increased density of the crystals greatly increases their resistance to starch-degrading enzymes. This is a promising method for preparing RS-rich products. © 2011 Elsevier Ltd. All rights reserved.


Deng Z.,Jiangnan University | Deng Z.,Hong Kong Polytechnic University | Choi K.-S.,Hong Kong Polytechnic University | Chung F.-L.,Hong Kong Polytechnic University | And 2 more authors.
Pattern Recognition | Year: 2010

While within-cluster information is commonly utilized in most soft subspace clustering approaches in order to develop the algorithms, other important information such as between-cluster information is seldom considered for soft subspace clustering. In this study, a novel clustering technique called enhanced soft subspace clustering (ESSC) is proposed by employing both within-cluster and between-class information. First, a new optimization objective function is developed by integrating the within-class compactness and the between-cluster separation in the subspace. Based on this objective function, the corresponding update rules for clustering are then derived, followed by the development of the novel ESSC algorithm. The properties of this algorithm are investigated and the performance is evaluated experimentally using real and synthetic datasets, including synthetic high dimensional datasets, UCI benchmarking datasets, high dimensional cancer gene expression datasets and texture image datasets. The experimental studies demonstrate that the accuracy of the proposed ESSC algorithm outperforms most existing state-of-the-art soft subspace clustering algorithms. © 2009 Elsevier Ltd. All rights reserved.


Chen A.-J.,Jiangnan University | Chen A.-J.,China National Control and Test Center for Packaging Quality
Zhendong yu Chongji/Journal of Vibration and Shock | Year: 2013

The variational iteration method was used to give an approximate solution to dropping shock problem of a cubic nonlinear packaging system. In order to raise the accuracy of the solution, a novel method combining the variational iteration method with the energy method was proposed. Using it, the maximum dropping shock response acceleration and displacement of the system were solved with the energy method, and the first-order iteration approximation solution was modified. The results show that the acceleration versus time and the displacement versus time curves obtained with this method are very similar to those using the elliptic integration method, and the accuracy of acceleration peak value and extended period of dropping shock obtained is satisfactory. The studying results provided a new method for dropping shock problem analysis of nonlinear packaging systems.


Wang J.,Jiangnan University
Mathematical Problems in Engineering | Year: 2013

He's inequalities and the Max-Min approach are briefly introduced, and their application to a coupled cubic nonlinear packaging system is elucidated. The approximate solution is obtained and compared with the numerical solution solved by the Runge-Kutta algorithm yielded by computer simulation. The result shows a great high accuracy of this method. The research extends the application of He's Max-Min approach for coupled nonlinear equations and provides a novel method to solve some essential problems in packaging engineering. © 2013 Jun Wang.


Wang H.-Y.,Jiangnan University | Gao Y.-B.,Jiangnan University | Fan Q.-W.,Jiangnan University | Xu Y.,Jiangnan University
Letters in Applied Microbiology | Year: 2011

Aims: To identify and compare microbiota in Chinese liquor Daqu, which were produced in the different regions using different production process. Methods and Results: The DNA exacted from Daqu samples was used as a template for PCR with universal primers of 16S rRNA, 26S rRNA and 18S rRNA, respectively. The amplicons were analysed using denaturing gradient gel electrophoresis (DGGE). It was observed that the bacterial DGGE profile indicated high diversity and predominance of lactic acid bacteria. The results showed that Saccharomycopsis fibuligera and Pichia anomal were dominant yeast species and that several non-Saccharomyces yeasts including Hanseniaspora guilliermondii, Debaryomyces hansenii, Issatchenkia orientalis and Trichosporon asahii were also detected. As for fungal DGGE, Aspergillus oryzae and Absidia blakesleeana were the most common species amongst different samples. Based on the DGGE analysis, a few differences in community structure were found between Daqu samples. Conclusions: A variety of bacteria, yeast and moulds were identified in Daqu samples, in addition to the present knowledge obtained mainly through the traditional culture-dependent methods. Moreover, production temperature played a more decisive role on the formation of micro-organism composition in Daqu than geographical region. Significance and Impact of the Study: PCR-DGGE technique was used in this study to fully observe and asses all microbial community (including bacteria, yeast and mould) in Chinese liquor Daqu for the first time and proved to be effective in profiling Daqu microbial diversity. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.


Luo J.,Jiangnan University | Zhang H.,Jiangnan University | Jiang S.,Jiangnan University | Jiang J.,Jiangnan University | Liu X.,Jiangnan University
Microchimica Acta | Year: 2012

We have developed a non-enzymatic glucose sensor by using a composite prepared from copper nanoparticles (CuNPs) and graphene which can be prepared by simple 1-step electrochemical reduction using graphene oxide (GO) and copper ion as the starting materials. The GO is electrochemically reduced to graphene at a voltage of -1. 5 V, and this is accompanied by the simultaneous formation of CuNPs on the surface of the graphene. This novel nanocomposite combines the advantages of graphene and of CuNPs and displays good electrocatalytic activity toward glucose in alkaline media. The performance of the respective glucose electrode was evaluated by amperometric experiments and revealed a fast response (<2 s), a low detection limit (200 nM), and high sensitivity (607 μA mM -1). The sensor also exhibits good reproducibility and very good specificity for glucose over ascorbic acid, dopamine, uric acid, fructose, lactose and sucrose. © 2012 Springer-Verlag.


Du L.,Jiangnan University | Zhang S.,Jiangnan University | Ding Y.,Jiangnan University
Zeitschrift fur Anorganische und Allgemeine Chemie | Year: 2012

A carbonate-bridged rhodium(III) dimeric complex of formula μ-CO 3-[(ppy) 2Rh] 2, (ppy = 2-phenylpyridine) was synthesized and characterized by IR and 1H NMR spectroscopy as well as X-ray diffraction. The bridging carbonate ion presumably originates from the capture of CO 2 in air deduced on a series of control experiments, which may have valuable implications for the study of fixation of CO 2. The luminescent and thermal properties of this complex were also investigated. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Liu X.,Jiangnan University | Sun C.-H.,University of Florida | Jiang P.,University of Florida
Chemistry of Materials | Year: 2010

Arrays of microcontainers, which allow handling and isolating a small volume of liquids, are of great technological importance in the miniaturization of analytical and bioanalytical techniques. Here we report a scalable bottom-up approach for fabricating wafer-sized, periodic arrays of metallic Petri dishes with a volume as small as 10 attoliter/dish. A monolayer, nonclose-packed colloidal crystal prepared, by a spincoating technology is first used as a structural template to create ordered microwells with vertical sidewalk Sputtering deposition of metals on the microwells, followed by removal of template, results in the formation of isolated metallic Petri, dishes. The size, separation, depth, thickness, and metal types of the resulting Petri dishes can be easily tuned by adjusting the size of the colloidal microspheres and the templating conditions. We have also demonstrated that, templated gold Petri dish arrays show strong surface-enhanced Raman scattering from adsorbed benzenethiol molecules. This bottom-up technology is compatible with standard microfabrication, promising for applications ranging from biomicroanalysis to surface plasmon devices. © 2010 American Chemical Society.


Ma W.,Jiangnan University | Yin H.,Jiangnan University | Xu L.,Jiangnan University | Wang L.,Jiangnan University | And 2 more authors.
Chemical Communications | Year: 2013

An ultrasensitive method for DNA detection based on magnetic assembly induced by polymerase chain reaction (PCR) was developed. The sensor showed a low limit of detection (LOD) of 4.26 aM with a wide range of target DNA from 0.01 fM to 10 000 fM. © 2013 The Royal Society of Chemistry.


Cheng L.,Jiangnan University | Mu W.,Jiangnan University | Jiang B.,Jiangnan University
Journal of the Science of Food and Agriculture | Year: 2010

Background: D-Tagatose, as one of the rare sugars, has been found to be a natural and safe low-calorie sweetener in food products and is classified as a GRAS substance. L-Arabinose isomerase (L-AI, EC 5.3.1.4), catalysing the isomerisations of L-arabinose and D-galactose to L-ribulose and D-tagatose respectively, is considered to be the most promising enzyme for the production of D-tagatose. Results: The araA gene encoding an L-AI from Bacillus stearothermophilus IAM 11001 was cloned, sequenced and over expressed in Escherichia coli. The gene is composed of 1491 bp nucleotides and codes for a protein of 496 amino acid residues. The recombinant L-AI was purified to electrophoretical homogeneity by affinity chromatography. The purified enzyme was optimally active at 65°C and pH 7.5 and had an absolute requirement for the divalent metal ion Mn2+ for both catalytic activity and thermostability. The enzyme was relatively active and stable at acidic pH of 6. The bioconversion yield of D-galactose to D-tagatose by the purified L-AI after 12 h at 65°C reached 36%. Conclusion: The purified L-AI from B. stearothermophilus IAM 11001 was characterised and shown to be a good candidate for potential application in D-tagatose production. © 2010 Society of Chemical Industry.


Lv Y.,Jiangnan University | Zhang X.,Jiangnan University | Zhang H.,Jiangnan University | Abbas S.,Jiangnan University | Karangwa E.,Jiangnan University
Food Hydrocolloids | Year: 2013

The coacervates of gelatin (G) and gum arabic (GA) were prepared in order to elucidate their pH-dependent complexation mechanism. Three biopolymers mixing ratios (MRs) (G/GA of 2:1, 1:1 and 1:2, w/w) were chosen to disclose their individual coacervates transition pattern for morphology and size distribution. The results showed that with pH decline, the coacervates became larger for the MR of 1:1 and 1:2; whereas, the trend went oppositely as to the MR of 2:1. Through the composition analysis of coacervates, such transition pattern was found to be consistent with the conversion rate of GA. Coacervates prepared by the MR of 2:1 were chosen to further investigate the formation mechanism at the molecular level. During the complexation process with pH decrease, G molecules experienced a conformational change from a flexible pattern to an ordered PPII helix. On the other hand, GA went through a transition from partly ordered PPII helix to relatively disordered conformation, and then converted to a more compact structure, called PPI helix. Such molecular transformation for both G and GA finally contributed to the smaller coacervates with pH decline, which coincided perfectly with the morphology evolution. © 2012 Elsevier Ltd.


Tian Y.,Jiangnan University | Zhan J.,Jiangnan University | Zhao J.,Jiangnan University | Xie Z.,Jiangnan University | And 2 more authors.
Food Hydrocolloids | Year: 2013

In this work, a dual-retrogradation treatment (gelatinization-retrogradation-gelatinization-retrogradation) was designed for preparing slowly digestible starch (SDS) products from rice starch. The results showed that a maximum SDS yield in the tested samples increased from 39.3% to 56.7% by the dual retrogradation with the time interval of 36 h. The dual retrogradation also seemed to increase with the melting temperature range (Tc-To) of the SDS products. This increase indicated that more imperfect crystallites were formed during the dual-retrogradation treatment. Furthermore, compared to the single retrogradation, the dual retrogradation generated larger cavities and more solid connection parts in the SDS products. This internal microstructure could result in the increase of slow digestibility. These findings suggest that the dual retrogradation can be used in starchy products to increase the yield of SDS. © 2012 Elsevier Ltd.


Xu L.,Jiangnan University | Wu L.,Jiangnan University
Journal of the Science of Food and Agriculture | Year: 2010

Background: The September 2008 melamine outbreak in China has adversely affected perceptions of food safety among consumers. This paper presents a survey of the perception of food safety and willingness to pay for certified traceable (CT) food among the citizens of Jiangsu Province. It presents the critical necessity of establishing a food traceability system (FTS) to address possible similar outbreaks in the future. Results: The results show that 36% of the respondents are strongly dissatisfied with food safety conditions in the province. Only 37% of the respondents have heard of FTS. Among the respondents who chose to buy CT food, 32% were unwilling to shoulder the extra cost. The consumers' overall satisfaction with food safety, awareness of FTS, gender, age, educational level and income are the main determinants of Chinese consumers' willingness to pay for CT food. Conclusion: Based on the results, it is proposed that the Chinese government pay attention to the mentality of its citizens when establishing an FTS and take measures to lift consumers' willingness to pay for CT food. Results of this study will provide valuable insights to developing countries. © 2010 Society of Chemical Industry.


Pan Z.,Jiangnan University
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2013

Negative information plays an important role in fuzzy knowledge representation and reasoning. This paper distinguish between contradictory negative relation and opposite negative relation for the fuzzy information, a characteristic of fuzzy information is discovered that if a pair of opposite concepts are fuzzy concepts, then there must exists a "medium" fuzzy concept between them; conversely, if there is a medium fuzzy concept between the two opposite concepts, then opposite concepts must be fuzzy concepts. We thus consider that negation of fuzzy information include contradictory negation, opposite negation and medium negation. In order to provide a logical basis for three kinds of negation in fuzzy information, we propose a fuzzy propositional logic with contradictory negation, opposite negation and medium negation (FLcom), discussed some interesting properties of FLcom, presented a semantic interpretation of FLcom, and proved the reliability theorem. © 2013 Springer-Verlag.


In this study, we investigated the effects and mechanisms involved in high pressure argon and xenon (HP Ar + Xe) mixed treatment on wound healing in fresh-cut apples and pineapples. Fresh-cut apples and pineapples treated by HP (1.8 MPa absolute) Ar and Xe (Ar:Xe = 2:9 partial pressure) were stored at 4 °C for 96 h. H 2O 2 production, the activities of related enzyme, the accumulation of phenolics and lignin were compared amongst those untreated and treated by HP air, normal atmosphere pressure (NAP Ar + Xe). The apple and pineapple samples subjected to the above treatments were inoculated with Escherichia coli and Saccharomyces cerevisiae, respectively, and incubated at 10 °C for one week. The growth of E. coli or S. cerevisiae was monitored to evaluate the wound-induced response to defense or the resistance against infection. Results showed that the HP (Ar + Xe) mixed treatment promoted the wound-healing response and enhanced resistance against the infection of E. coli or S. cerevisiae in fresh-cut apples and pineapples. The enhanced wound healing ability in fruit pieces subjected to HP (Ar + Xe) mixed treatment is found to be associated with the increase in H 2O 2 production and the accumulation of phenolics and lignin during storage after treatment. © 2012 Elsevier Ltd.


Zhang Y.,Jiangnan University | Zhang Y.,Nanjing University | Feng B.,Jiangnan University | Zhu C.,Nanjing University
Organic and Biomolecular Chemistry | Year: 2012

Au(iii)-catalyzed intermolecular amidations of benzylic C-H bonds with sulfonamides and carboxamides are described. The protocol with the Au-bipy complex/N-bromosuccinimide system provides practical applications for synthesis of various amides via C-H activations. The reaction proceeds with high efficiency to give the corresponding amines, which are extremely useful synthetic intermediates. © 2012 The Royal Society of Chemistry.


Lv Z.Y.,Jiangnan University
Applied Mechanics and Materials | Year: 2014

As the reform of construction industry and construction management system in our country is continuously deepening, the management methods and organization structure on production had been deeply changed for enterprises in construction industry, which has gradually established a "modern enterprise system" and vigorously promoting "construction of project methods" on the construction management in construction works. In this paper, by reviewing and discussing the latest research results on construction safety management in and abroad, the key research content and subject focused in current safety management were proposed. Two management technical methods on safety objective management applicable to local construction projects were designed and improved. One is contractual safety responsibility method; the other is overall safety quality management method. The application effects were tested, analyzed and improved by using quantitative safety comprehensive evaluation method to achieve a systemic and scientific safety management theory system that is complete and practical. © (2014) Trans Tech Publications, Switzerland.


The objective of the study was to establish disulphide interaction between protein-coated oil droplets and the surrounding protein matrix in myofibrillar protein (MP)-emulsion composite gels. An MP-stabilized peanut oil emulsion was treated with 0, 1, 3, 5 and 10. mM N-ethylmaleimide (NEM, a sulphydryl-blocking agent) and subsequently incorporated into a bulk MP sol to produce 5%-lipid, 2%-protein composites at pH 6.2. About 69% of sulphydryls in the emulsion (1% protein) were blocked by 1. mM NEM, and almost all were bound at ≥ 3. mM NEM. The loss of free sulphydryls resulted in a significant drop in the storage modulus (G') and rupture force of the composite gels. Microstructural examination revealed pores and oil leakage from emulsion droplets by NEM treatments, corresponding to declining rheological properties of the MP-emulsion composites. The results supported the hypothesis that disulphide cross-linking between MP-coated oil droplets and protein matrix contributed to the stabilization and reinforcement of protein-emulsion composite gels formed in comminuted muscle foods. © 2011 Elsevier Ltd.


Chen B.,Jiangnan University | Wu Q.,Jiangnan University | Xu Y.,Jiangnan University
International Journal of Food Microbiology | Year: 2014

Maotai-flavor liquor is produced by simultaneous saccharification and fermentation (SSF) process under solid state conditions, including Daqu (starter) making, stacking fermentation and alcohol fermentation stages. Filamentous fungi produce many enzymes to degrade the starch material into fermentable sugar during liquor fermentation. This study investigated the filamentous fungal community associated with liquor making process. Eight and seven different fungal species were identified by using culture-dependent and -independent method (PCR-denaturing gradient gel electrophoresis, DGGE) analyses, respectively. The traditional enumeration method showed that Daqu provided 7 fungal species for stacking fermentation. The total population of filamentous fungi increased from 3.4×103cfu/g to 1.28×104cfu/g in the first 3days of stacking fermentation, and then decreased till the end. In alcohol fermentation in pits, the population continuously decreased and few fungal species survived (lower than 1×103cfu/g) after 10days. Therefore, stacking fermentation is an essential stage for the growth of filamentous fungi. Paecilomyces variotii, Aspergillus oryzae and Aspergillus terreus were detected by both methods, and P. variotii and A. oryzae were the predominant species. Meanwhile, P. variotii possessed the highest glucoamylase (3252±526U/g) and A. oryzae exhibited the highest α-amylase (1491±324U/g) activity among the cultivable fungal species. Furthermore, the variation of starch and reducing sugar content was consistent with the growth of P. variotii and A. oryzae in Zaopei (fermented grains) during stacking fermentation, which implied that the two filamentous fungi played an important role in producing amylase for hydrolyzing the starch. © 2014 Elsevier B.V.


Pan H.,Jiangnan University | Jiang B.,Jiangnan University | Chen J.,Jiangnan University | Jin Z.,Jiangnan University
Food Chemistry | Year: 2014

Different types of polysaccharides (propyleneglycol alginate (PGA), pectin, carrageenan and aloe polysaccharide) were incorporated into soy protein isolate (SPI)/lauric acid (La) films using a co-drying process or by direct addition to form biodegradable composite films with modified water vapour permeability (WVP) and mechanical properties. The WVP of SPI/La/polysaccharide films decreased when polysaccharides were added using the co-drying process, regardless of the type of polysaccharide. The tensile strength of SPI/La film was increased by the addition of polysaccharides, and the percentage elongation at break was increased by incorporating PGA using the co-drying process. Regarding oxygen-barrier performance, no notable differences were observed between the SPI/La and SPI/La/polysaccharide films. The most significant improvement was observed by blending PGA, with the co-dried preparation exhibiting better properties than the direct-addition preparation. Scanning electron microscopy (SEM) revealed that the microstructures of the films are the basis for the differences in the barrier and mechanical properties of the modified blends of SPI, polysaccharides and La. © 2013 Elsevier Ltd. All rights reserved.


Xiao Q.,Hunan Agricultural University | Tong Q.,Jiangnan University | Lim L.-T.,University of Guelph
Food Chemistry | Year: 2014

Drying phenomenon of aqueous pullulan solutions at 50 C was investigated by ATR-FTIR spectroscopy and two-dimensional correlation infrared spectroscopy. Two-dimensional asynchronous spectrum at 1800-1500 cm-1 wavenumber was resolved into three separate bands, which were assigned to aggregated water with strong hydrogen bond (AW-S), aggregated water with moderately hydrogen (AW-M) bond, and free water (FW), respectively. Curve fitting results indicated that the quantities of the three species of water were constant during the first 55 min of drying process. However, the area of the three bands (at around 1047, 1022 and 998 cm-1) varied as drying progressed, implying the formation of more-ordered pullulan chains structure, as well as increased pullulan chain-chain interactions. Further analysis using 2D asynchronous correlation spectroscopy in 1200-950 cm-1 wavenumber regions revealed the sequence of spectral changes during the drying process. © 2013 Elsevier Ltd. All rights reserved.


Ruan Q.,Jiangnan University | Chen Y.,Jiangnan University | Kong X.,Jiangnan University | Hua Y.,Jiangnan University
Food Chemistry | Year: 2014

In this study, soy proteins were reduced with 0.1-10 mM dithiothreitol (DTT) to obtain an increasing number of sulphydryl groups (SH) with a similar particle size. Aggregation was promoted by increasing the degree of reduction when heated (100 C, 30 min), resulting in larger sized aggregates (from 40 to 70 nm) and a higher viscosity of the aggregate dispersion. The disulphide bond (SS) content decreased and the less SS linked polymer, which was composed of acidic (A) polypeptide of glycinin, basic (B) polypeptides of glycinin, and a small amount of α′ and α subunits of β-conglycinin, was formed with increasing reduction degree, suggesting that SH/SS polymerisation was not the driving force for aggregation. The larger aggregates with increasing degrees of reduction were composed of more B of glycinin and β of β-conglycinin, suggesting that the A and the small amount of α′ and α in the SS linked polymer have an inhibiting effect on protein aggregates formation. © 2014 Elsevier Ltd. All rights reserved.


Lu T.,Jiangnan University | Xia Y.-M.,Jiangnan University
Food Chemistry | Year: 2014

Specificity of glycosyl donors is a critical issue in transglycosylation of stevioside, the main methodology to improve edulcorant quality of stevioside. The most popular glucanotransferase applied in this reaction is cyclodextrin glucanotransferase (CGTase) that catalyses cyclisation, coupling, hydrolysis and disproportionation simultaneously; which results a crosstalk in the glycosyl donors that comes from initial reactants, reaction intermediates as well as side products in parallel reactions. In this work, the specificity of glycosyl donors was studied to understand the transglucosylation pathways with the designed experiments and material balance analysis on the products using a commercial CGTase. It has been found that cyclodextrins and starches provided the best transglucosylation yield, while the assayed mono- and disaccharides were not effective glycosyl donors to stevioside with the CGTase. It is proposed that α- and β-cyclodextrins performed transglycosylation via coupling to produce intermediates of reducing sugar and followed by disproportionation with stevioside; while starches may perform the transglycosylation combined the cyclodextrins pathway and hydrolysis pathway of starches. © 2014 Elsevier Ltd. All rights reserved.


Liu J.,Jiangnan University | Xu Y.,Jiangnan University | Zhao G.,Jiangnan University
Journal of the Institute of Brewing | Year: 2012

Ethyl carbamate (EC) is a naturally occurring toxic contaminant that may imply a risk to human health and is usually found in alcoholic beverages such as Chinese rice wine. An automated procedure for the rapid determination of EC in Chinese rice wine was developed by headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS). Using propyl carbamate as internal standard, the optimized HS-SPME sampling with a polyacrylic fibre was 45 min at 70°C after applying 38.8% NaCl to saturate the sample. This method showed good linearity over a range of 25-600μgL-1 (R2 = 0.997). The recovery, relative standarddeviation and limit of detection were 90.21-97.35%, lower than 2.89% and 1.19μg L-1,respectively. Additionally, the ethanol concentration had no effect on the analysis of EC. The total analysis time of 57min per sample in continuous determination was twice as fast as the widely used solid-phase extraction-GC-MS method. This solventfree HS-SPME-GC-MS procedure is suitable for the rapid, automated, and therefore convenient, determination of EC in Chinese rice wine. © 2012 The Institute of Brewing & Distilling.


Shen Q.,Jiangnan University | Ding F.,Jiangnan University
Nonlinear Dynamics | Year: 2014

This paper considers iterative identification problems for a Hammerstein nonlinear system which consists of a memoryless nonlinear block followed by a linear dynamical block. The difficulty of identification is that the Hammerstein nonlinear system contains the products of the parameters of the nonlinear part and the linear part, which leads to the unidentifiability of the parameters. In order to obtain unique parameter estimates, we express the output of the system as a linear combination of all the system parameters by means of the key-term separation principle and derive a gradient based iterative identification algorithm by replacing the unknown variables in the information vectors with their estimates. The simulation results indicate that the proposed algorithm can work well