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Chen J.,Guilin Medical University | Lin C.,Jiangbin Hospital of Guangxi Zhuang Autonomous Region | Yong W.,Guilin Medical University | Ye Y.,Guangxi Medical University | Huang Z.,Guilin Medical University
Cellular Physiology and Biochemistry | Year: 2015

Background: Calycosin and genistein are the two main components of isoflavones. Previously, we reported that these compounds display antitumor activities in the breast cancer cell lines MCF-7 and T47D. In the present study, we investigated the mechanism of action of calycosin and genistein, and their respective efficacies as potential therapies for the treatment of breast carcinoma in the clinic. Methods: MCF-7 cells were treated with calycosin or genistein. Cell proliferation and apoptosis were measured using CCK8 assay and Hoechst 33258. The expression level of phosphorylated Akt protein was determined by western blotting. Expression level of HOTAIR was quantified by real-time PCR. Results: Both calycosin and genistein inhibited proliferation and induced apoptosis in MCF-7 breast cancer cells, especially after treatment with calycosin. Treatment of MCF-7 cells with calycosin or genistein resulted in decreased phosphorylation of Akt, and decreased expression of its downstream target, HOTAIR. Conclusion: Calycosin is more effective in inhibiting breast cancer growth in comparison with genistein, through its regulation of Akt signaling pathways and HOTAIR expression. © 2015 S. Karger AG, Basel. Source


Xu Y.-B.,Jiangbin Hospital of Guangxi Zhuang Autonomous Region | Bai Y.-L.,Guangxi Medical University | Min Z.-G.,Xian Jiaotong University | Qin S.-Y.,Guangxi Medical University
World Journal of Gastroenterology | Year: 2013

Aim: To establish the role of magnetic resonance cholangiography (MRC) in diagnosis of biliary anatomy in living-donor liver transplantation (LDLT) donors. Methods: A systematic review was performed by searching electronic bibliographic databases prior to March 2013. Studies with diagnostic results and fulfilled inclusion criteria were included. The methodological quality of the studies was assessed. Sensitivity, specificity and other measures of the accuracy of MRC for diagnosis of biliary anatomy in LDLT donors were summarized using a random-effects model or a fixed-effects model. Summary receiver operating characteristic (SROC) curves were used to summarize overall test performance. Publication bias was assessed using Deek's funnel plot asymmetry test. Sensitivity analysis was adopted to explore the potential sources of heterogeneity. Results: Twelve studies involving 869 subjects were eligible to the analysis. The scores of Quality Assessment of Diagnostic Accuracy Studies for the included studies ranged from 11 to 14. The summary estimates of sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic OR of MRC in diagnosis of biliary anatomy in LDLT donor were 0.88 (95%CI: 0.84-0.92), 0.95 (95%CI: 0.93-0.97), 15.33 (95%CI: 10.70-21.95), 0.15 (95%CI: 0.11-0.20) and 130.77 (95%CI: 75.91-225.27), respectively. No significant heterogeneity was detected in all the above four measures. Area under SROC curve was 0.971. Little publication bias was noted across the studies (P = 0.557). Sensitivity analysis excluding a study with possible heterogeneity got a similar overall result, which suggested the little influence of this study on the overall results. Conclusion: Our results suggest that MRC is a high specificity but moderate sensitivity technique in diagnosis of biliary anatomy in LDLT donors. © 2013 Baishideng Publishing Group Co., Limited. All rights reserved. Source


Xu Y.-B.,Jiangbin Hospital of Guangxi Zhuang Autonomous Region | Min Z.-G.,Xian Jiaotong University | Jiang H.-X.,Guangxi Medical University | Qin S.-Y.,Guangxi Medical University | Hu B.-L.,Guangxi Medical University
Transplantation Proceedings | Year: 2013

Background and Objective Magnetic resonance cholangiopancreatography (MRCP) is a noninvasive procedure to diagnose biliary complications. The aim of the present meta-analysis was to establish the overall diagnostic accuracy of MRCP to diagnose biliary complications post-orthotopic liver transplantation (OLT). Methods A systematic review was performed by searching electronic bibliographic databases prior to May 2012. Sensitivity, specificity, and other measures of the accuracy of MRCP for diagnosis of post-OLT were summarized using a random-effects or a fixed-effects model. Receiver operating characteristic curves were used to summarize overall test performance. Results Fourteen studies, which involved 892 subjects were eligible for the analysis. The summary estimates of sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and area under cure of MRCP for diagnosis of biliary complications were as follows: 0.95, 0.92, 10.23, 0.08, 206.59, and 0.979, respectively. The results for biliary strictures in four studies involving 177 subjects were 0.94, 0.95, 0.96, 0.09, 178.33, and 0.973 respectively. Conclusions MRCP is a sensitive and specific technique to diagnose biliary complications. © 2013 Elsevier Inc. Source


Feng J.,Peking Union Medical College | Feng J.,Key Laboratory of Geriatrics | Liu M.,Peking Union Medical College | Liu M.,Key Laboratory of Geriatrics | And 7 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: MtDNA haplogroup D has been shown to influence longevity trait. However, the effects that mtDNA haplogroup D (and its subhaplogroups) have upon longevity in Southern Chinese population have rarely been reported, and the effect of reaction of mtDNA haplogroup D (and its subhaplogroup) and apolipoprotein E (APOE) gene on longevity remains poorly understood. OBJECTIVE: To explore the association between mtDNA haplogroup D (and its subhaplogroup) and longevity in southern Chinese population, and the interaction of mtDNA haplogroup D (and its subhaplogroup) with APOE gene. METHODS: A total of 1 038 people were selected from Bama, Guangxi between 2007 and 2008, including 367 long-lived individuals (aged > 90 years) and 671 local and unrelated younger controls (aged 40-60 years). Using polymerase chain reaction-restriction fragment length polymorphism, the distribution of mtDNA haplogroup D and its subhaplogroup (D4, D4a) was tested. The confounding effect of gender and APOE gene was detected by stratification analysis. RESULTS AND CONCLUSION: There was no significant correlation between mtDNA haplogroup D (and its subhaplogroup) and longevity (P > 0.05). Stratification analysis by gender showed no significant difference for frequencies distribution of mtDNA haplogroup D and its subhaplogroup (D4, D4a) between long-lived individuals and controls in women and men (P > 0.05). However, the frequency distribution of mtDNA haplogroups D was different between long-lived individuals and controls with APOE e4 carriers (P < 0.05) through stratification analysis by APOE gene. Results show that mDNA haplogroup D may affect longevity by interacting with APOE gene. Source


Luo C.-Y.,Guangxi Medical University | Liu C.-W.,Guangxi Medical University | Ge L.,Guangxi Medical University | Pang G.-F.,Jiangbin Hospital of Guangxi Zhuang Autonomous Region | And 10 more authors.
Lipids in Health and Disease | Year: 2015

Background: The +294T/C polymorphism in the peroxisome proliferator-activated receptor delta (PPARD) gene is associated with hyperlipidemia in several younger populations, but results are still inconsistence across ethnic groups and its possible impact on the lipid profiles of long-lived individuals remains unexploited. Here, we aimed to evaluate the possible correlation between PPARD +294T/C and serum lipid levels in a long-lived population in Bama, a region known for longevity situated in Guangxi, China. Methods: Genotyping of PPARD +294T/C polymorphism was conducted in 505 long-lived inhabitants (aged 90 and above, long-lived group, LG) and 468 healthy controls (aged 60-75, non-long-lived group, non-LG) recruited from Bama area. Results: No difference in allelic and genotypic frequencies was found between the two groups (P∈>∈0.05). However, C-allele and C-genotype (TC and CC) were significantly more frequent in the females of non-LG than were LG after sex stratification. CC carriers exhibited higher LDL-C level in LG (P∈<∈0.05) but lower TC, TG and LDL-C in non-LG (P∈<∈0.05 for each) than TT carriers; C allele carriers (TC/CC) in LG exhibited higher TC, TG, and LDL-C levels as compared with the same genotype and the same lipid parameter in non-LG (P∈<∈0.05 for each). LDL-C in LG was correlated with genotypes while TC, TG, and LDL-C in non-LG were correlated with genotypes (P∈<∈0.05-0.001). Conclusion: Our results suggest that there were different impact patterns of PPARD +294T/C polymorphism on lipid profiles between long-lived cohort and average population in Bama area and this may be one of the genetic bases of its longevity. © 2015 Luo et al.; licensee BioMed Central. Source

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