Wu J.,Maternal and Child Health Hospital of Jinan City |
Cai C.,Shanghai University |
Tong D.,Shanghai JiaoTong University |
Hou H.,Jian Guo
Genetic Testing and Molecular Biomarkers | Year: 2012
Despite the knowledge of many genetic alterations present in ovarian cancer, the complexity of this disease precludes placing its biology into a simple conceptual framework. Lysyl oxidase (LOX) is an extracellular matrix enzyme that catalyzes the cross-linking of collagens or elastin in the extracellular compartment. A novel polymorphism in the LOX gene, G473A (rs1800449), has been reported as being a risk factor for different diseases. The objective of this study was to investigate the association between the LOX G473A polymorphism and the susceptibility to ovarian cancer in the Chinese population. The LOX variant G473A was detected by a polymerase chain reaction-restriction fragment length polymorphism in 233 ovarian cancer cases and 246 age-matched controls. Data were analyzed using the chi-square test. Data showed that frequencies of the LOX 473AA genotype and the A allele were significantly higher in ovarian cancer patients than in controls (odds ratio [OR]=2.52, 95% confidence interval [CI] 1.28-4.96, p=0.006; and OR=1.62, 95% CI 1.18-2.20, p=0.002). In addition, the prevalence of the GA genotype, AA genotype, and A allele were significantly increased in recurrent ovarian cancer cases compared with primary ovarian cancer cases. Our data suggest that the G473A polymorphism of the LOX gene is associated with increased susceptibility to ovarian cancer. © 2012 Mary Ann Liebert, Inc.
Tie C.,CAS Beijing National Laboratory for Molecular |
Zhang D.-W.,CAS Beijing National Laboratory for Molecular |
Chen H.-X.,Jian Guo |
Song S.-L.,Beijing Center for Drug Abuser |
Zhang X.-X.,CAS Beijing National Laboratory for Molecular
Journal of Mass Spectrometry | Year: 2012
With the combination of high separation ability of capillary electrophoresis (CE) and strong identification ability of mass spectrometry (MS), CE/MS is becoming a powerful tool for polar and ionic analytes analysis. Different interfaces have been developed to enhance the sensitivity and reliability since the first introduction of CE/MS in 1987. A sheathless porous interface based on a new ions transferring electric connection technique was reported to be with high sensitivity and reliability. In this work, a series of optical and electrochemical experiments were designed to study the electric connection process. The results indicated that closing CE electrical circuit and applying MS spray voltage were achieved by the small ions transferring through the interface porous wall. The new electric connection method significantly enhanced the sensitivity, resolution and stability of the CE/MS analysis. The interface was applied in CE/MS detection of morphine and 6-monoacetylmorphine in urine sample and showed an equal sensitivity to LC/MS. With the significant improvement of sensitivity and stability, the CE/MS with the new interface showed strong potential for the determination of low abundance analytes. Copyright © 2012 John Wiley & Sons, Ltd.
Liu X.,Shandong University |
Han Q.,Shandong University |
Leng J.,Jian Guo
BMC Infectious Diseases | Year: 2014
Background: The innate immune system recognizes pathogens via its pattern recognition receptors. The objective of this study was to investigate the role of the nucleotide-binding oligomerization domain (NOD) proteins, a family of the novel bacterial pattern recognition receptors, in host responses to the gram-positive bacteria Streptococcus pneumoniae. Methods: Sprague-Dawley rats were infected via intracisternal injections of viable S. pneumoniae, and rats in the control group were injected with sterile saline. After infection, real-time PCR was performed to determine the presence of mRNAs encoding NOD1 and NOD2. Quantitative analyses of the NOD1, NOD2 and NF-kB proteins were also performed western blotting following challenge infections with viable S. pneumoniae. The TNF-a and IL-6 levels in brain homogenates were evaluated using enzyme-linked immunosorbent assays (ELISAs). Results: The results revealed up-regulations of the mRNA and protein levels of NOD2 within the CNS of rats with S. pneumoniae meningitis. Moreover, the activation of NF-ΚB in the brain tissues following infection with live S. pneumoniae was also significantly increased, which indicates that NOD2 mediated NF-ΚB activation in experimental pneumococcal meningitis. Similarly, TNF-a and IL-6 levels were increased in the brain following in vivo S. pneumoniae administration. Conclusions: These results suggest that NOD2 is involved in the host response to the gram-positive bacteria S. pneumoniae in the CNS and that NOD2 might play an important role in the initiation and/or progression of CNS inflammation associated with pneumococcal meningitis. © 2014 Liu et al.
Su C.-C.,Buddhist Tzu Chi General Hospital |
Su C.-C.,Tzu Chi University |
Wang M.-J.,Buddhist Tzu Chi General Hospital |
Wang M.-J.,Jian Guo |
And 2 more authors.
International Journal of Molecular Medicine | Year: 2010
Curcumin has been verified as an anti-cancer compound via multiple molecular targets. Its effective mechanisms include cell cycle arrest, inducing apoptosis, suppressing oncogenes, and enhancing tumor suppressor genes. The resistance of cells to chemotherapy, however, derives from the variable genetic aberration of cancer cells. Consequently, the core signaling pathways of glioblastoma have been explored to evaluate the efficacy of curcumin in proceeding through mutated genes in those pathways. In this study, the efficacy of curcumin was investigated in DBTRG cells. The cytotoxic ability was detected with MTT assay, and the influence of the cell cycle was checked with flow cytometry. The influence of the core signaling pathways was evaluated by Western blotting through the predominantly mutated proteins which included p53, p21, and cdc2 in the p53 pathway, CDKN2A/p16 and RB in the RB pathway, and EGFR, mTOR, Ras, PTEN, and Akt in the RTK-Ras-PI3K pathway. In addition, the apoptotic effect was determined by apoptosis-associated proteins Bcl-2, Bax, and caspase 3. Curcumin exhibits superior cytotoxicity on glioblastoma in a dose- and time-dependent manner in the MTT assay. In the core signaling pathways of glioblastoma, curcumin either significantly influences the p53 pathway by enhancing p53 and p21 and suppressing cdc2 or significantly inhibits the RB pathway by enhancing CDKN2A/p16 and suppressing phosphorylated RB. In the apoptotic pathway, the Bax and caspase 3 are significantly suppressed by curcumin and the Giemsa stain elucidates apoptotic features of DBTRG cells as well. In conclusion, curcumin appears to be an effective anti-glioblastoma drug through inhibition of the two core signaling pathways and promotion of the apoptotic pathway.
Simjoo M.,Sahand University of Technology |
Simjoo M.,Technical University of Delft |
Dong Y.,Technical University of Delft |
Dong Y.,Jian Guo |
And 3 more authors.
Industrial and Engineering Chemistry Research | Year: 2013
A systematic CT-scan-Aided laboratory study of N2 foam in Bentheimer sandstone cores is reported. The aim of the study was to investigate whether foam can improve oil recovery from clastic reservoirs subject to immiscible gas flooding. Foam was generated in situ in water-flooded sandstone cores by coinjecting gas and surfactant solution at fixed foam quality. It was stabilized using two surfactants, namely, C14-16 α-olefin sulfonate (AOS) and mixtures of AOS and a polymeric fluorocarbon (FC) ester. The effects of surfactant concentration, injection direction, surfactant preflush, and core length on foam behavior were examined in detail. Stable foams were obtained in the presence of waterflood residual oil. It was found that foam strength (mobility reduction factor) increases with surfactant concentration. Foam development and, correspondingly, oil recovery without surfactant preflush were delayed compared to the case with preflush. Gravity-stable foam injection caused a rapid increase in foam strength and an incremental oil recovery almost twice that for unstable flow conditions. Core floods revealed that the incremental oil recovery by foam was as much as (23 ± 2)% of the oil initially in place after injection of 4.0 pore volumes (PV) of foam (equal to the injection of 0.36 PV of surfactant solution). Incremental oil recovery was only (5.0 ± 0.5)% for gas flooding under the same injection conditions. It appears that oil production by foam flooding occurs by the following main mechanisms: (1) residual oil saturation to foam flooding is lower than that to water flooding; (2) formation of an oil bank in the first few injected pore volumes, coinciding with a large increase of capillary number; and (3) a long tail production due to the transport of tiny oil droplets within the flowing foam at a fairly constant capillary number. The observations of this study support the concept that foam is potentially an efficient enhanced oil recovery (EOR) method. © 2013 American Chemical Society.