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PubMed | Jeonnam Biofood Technology Center, Chonnam National University and Korea Basic Science Institute
Type: Journal Article | Journal: Planta medica | Year: 2016

Hyperforin, a major active compound of St.Johns wort extract, affects estrogenic activity. In this study, the compound evoked estrogen response element-dependent luciferase activity and cell proliferation in MCF-7 cells. Hyperforin-induced cell proliferation was significantly inhibited by the estrogen receptor antagonist ICI 182,780. These results suggested that hyperforin had estrogenic and cell proliferation activities, which were stimulated via the estrogen receptor. Compared to 17

PubMed | Korea Polytechnic University, Chonnam National University, Jeonnam Biofood Technology Center, Zhengzhou University and 3 more.
Type: Journal Article | Journal: International journal of oncology | Year: 2015

The World Health Organization (WHO) has reported that cancer is one of the most prevalent diseases and a leading cause of death worldwide. Many anticancer drug development studies have been pursued over the last few decades and several viable drugs have been discovered, such as paclitaxel, topotecan and irinotecan. Previously, our research group uncovered the cytocidal and cytostatic effects of the plant Stephania delavayi Diels. In this study, we determined the active chemical to be 6,7-di-O-acetylsinococuline (FK-3000). The FK-3000 half maximal inhibitory concentration (IC50) in MDA-MB-231 breast carcinoma cells at 48 h was 0.52 g/ml and it induced apoptosis in a dose- and time-dependent manner. FK-3000 suppressed NF-B nuclear translocation, decreased NF-B phosphorylation, and decreased COX-2 protein expression. MDA-MB-231 xenografted mice were treated with FK-3000, Taxol, or their combination for 21 days. The tumor size was smallest in the co-treatment group, indicating that FK-3000 may have a synergistic effect with Taxol. FK-3000 treatment showed no adverse effects on blood cell counts, serum protein levels, or pathology. These studies demonstrate that FK-3000, isolated from S. delavayi Diels., is a promising, pathway-specific anticancer agent that exhibits low toxicity.

PubMed | Jeonnam Biofood Technology Center, Korea Institute of Toxicology, Dongshin University, Korea Research Institute of Bioscience and Biotechnology and Kyungpook National University
Type: Journal Article | Journal: PloS one | Year: 2015

According to the World Health Organization in 2013, 235 million people are afflicted with asthma. Asthma is a severe pulmonary disease that can be caused by the imbalance of T-helper (Th) type 1 (Th1) and type 2 (Th2) cells, and it is potentially fatal. In this study, we evaluated the anti-asthmatic effect of alginate oligosaccharide (AO), which was prepared from seaweed and converted by Bacillus subtilis KCTC 11782BP, in the mouse model of ovalbumin (OVA)-induced asthma. BALB/c mice were divided into the vehicle control (sensitized but not challenged), asthma induction, positive control (1 mg/kg dexamethasone), 50 mg/kg/day AO-treated, 200 mg/kg/day AO-treated, and 400 mg/kg/day AO-treated groups. The numbers or levels of inflammatory cells, eosinophils, and immunoglobulin (Ig) E were measured in bronchoalveolar lavage fluid (BALF), and asthma-related morphological and cytokine changes were analyzed in lung tissues. Our results show that AO dramatically reduced inflammatory cell numbers, eosinophil count, and IgE levels in BALF, and it dose-dependently inhibited asthmatic histopathological changes in the lung. In addition, AO dose-dependently suppressed the expression of CD3+ T-cell co-receptors, CD4+ Th cells, CD8+ cytotoxic T-cell-related factors, macrophages, and MHCII class. AO dose-dependently decreased the expression levels of Th1/2 cells-regulatory transcription factors such as GATA-3 which modulates Th2 cell proliferation and T-bet which does Th1 cell proliferation. The mRNA levels of all Th1/2-related cytokines, except IL-12, were dose-dependently suppressed by AO treatment. In particular, the mRNA levels of IL-5, IL-6, and IL-13 were significantly inhibited by AO treatment. Our findings suggest that AO has the potential to be an anti-asthmatic drug candidate, due to its modulation of Th1/Th2 cytokines, which contribute to the pathogenesis of asthma.

Cho S.Y.,Korea University | Park M.J.,Korean University of Science and Technology | Kim K.M.,Jeonnam Biofood Technology Center | Ryu J.-H.,Korean University of Science and Technology | Park H.J.,Korean University of Science and Technology
Food Science and Biotechnology | Year: 2011

A sour kimchi product with an elevated amount of γ-aminobutyric acid (GABA) was produced using starter lactic acid bacteria (LAB) for mukeunjee kimchi fermentation. The starter LAB were screened and isolated from the commercial mukeunjee kimchi product that showed the highest GABA content and was identified as Lactobacillus buchneri. The maximum GABA production of L. buchneri in MRS media was 5.83 mg/mL at pH 4.2 and the addition of 3% NaCl did not significantly (p>0.05) changed GABA production of L. buchneri. The amount of GABA in L. buchneri-inoculated sour kimchi was 61.65 mg/100 g, which represented about 8 times higher than the L. buchneri uninoculated kimchi (control kimchi). A sensory evaluation test of L. buchneri-inoculated sour kimchi and control kimchi showed that the L. buchneri inoculation may produce more sour kimchi than control kimchi, but there was no difference in the general acceptance between the 2 kimchi products (p>0.05). © KoSFoST and Springer 2011.

Ko B.-K.,Korea University | Kim K.M.,Jeonnam Biofood Technology Center | Hong Y.-S.,Korea University | Lee C.-H.,Korea University
Journal of Agricultural and Food Chemistry | Year: 2010

Meju, a brick of dried fermented soybean naturally inoculated with microorganisms, is a starter used for producing traditional Korean fermented soybean products such as soybean paste (doenjang) and soy sauce (ganjang). In order to reduce aging time during production of soybean paste and soy sauce, high pressure (HP) treatment was applied to the meju starter at 500 MPa of pressure for 10 min at 15 °C. Fermentative behaviors of normal and HP-treated meju were assessed and compared through physicochemical and 1H NMR-based metabolomic analysis. All mejues were incubated for 3 weeks at 30 °C. At 1 week of incubation, total bacterial population decreased mainly due to a reduction of water content by spontaneous evaporation during the incubation period. As the incubation time increased, glutamate, proline, betain, choline, and phosphocholine levels increased in both normal and HP-treated mejues, indicating that microorganisms in the mejues synthesize these metabolites to endure intracellular hyperosmotic stress induced by the reduction in water content. Through 3 weeks of incubation, the amino-type nitrogen contents and neutral protease activities in HP-treated meju were significantly higher (p < 0.05) than in normal meju, even though total bacterial content in HP-treated meju was 2 or 3 times lower. Moreover, marked increases in glycerol, acetate, tyrosine, and choline levels were observed in HP-treated meju compared to normal meju. In particular, higher levels of tyrosine in HP-treated meju were consistent with the increased neutral protease activities compared to normal meju, indicating an improvement in enzyme stability with HP treatment. These findings highlight a new or better understanding of the influence of the HP or physical treatments on fermentative products in food processing, such as those associated with soybean paste and soy sauce, regarding metabolic behaviors in fermentative starter induced by HP treatment. © 2010 American Chemical Society.

Rhyu J.,Mokpo National University | Kim M.S.,Mokpo National University | You M.-K.,Mokpo National University | Bang M.-A.,Jeonnam Biofood Technology Center | Kim H.-A.,Mokpo National University
Nutrition Research and Practice | Year: 2014

Obesity occurs when a person's calorie intake exceeds the amount of energy burns, which may lead to pathologic growth of adipocytes and the accumulation of fat in the tissues. In this study, the effect and mechanism of pear pomace extracts on 3T3-L1 adipocyte differentiation and apoptosis of mature adipocytes were investigated. The effects of pear pomace extract on cell viability and the anti-adipogenic and proapoptotic effects were investigated via MTT assay, Oil red O staining, western blot analysis and apoptosis assay. 3T3-L1 preadipocytes were stimulated with DMEM containing 10% FBS, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 5 μg/ml insulin and 1 μM dexamethasone for differentiation to adipocytes. 3T3-L1 cells were cultured with PBS or water extract of pear pomace. Water extract of pear pomace effectively inhibited lipid accumulations and expressions of PPAR-γ and C/EBPα in 3T3-L1 cells. It also increased expression of p-AMPK and decreased the expression of SREBP-1c and FAS in 3T3-L1 cells. The induction of apoptosis was observed in 3T3-L1 cells treated with pear pomace. These results indicate that pear pomace water extract inhibits adipogenesis and induces apoptosis of adipocytes and thus can be used as a potential therapeutic substance as part of prevention or treatment strategy for obesity. © 2014 The Korean Nutrition Society.

PubMed | Jeonnam Biofood Technology Center, Mokpo National University and Nature Pure Korea Inc. Jeonnam 517 803
Type: Journal Article | Journal: Nutrition research and practice | Year: 2015

In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231 human breast cancer cell line was determined.Cells were cultured with DMSO or with 50, 75, 100 or 250 g/ml of Erythronium japonicum methanol or ethanol extract.Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion of breast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2 and MMP-9 activities were also inhibited by both methanol and ethanol extracts.Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migration and invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.

Lee J.-E.,Korean University of Science and Technology | Kim K.M.,Jeonnam Biofood Technology Center
Journal of Applied Polymer Science | Year: 2010

Soy protein isolate/montmorillonite (SPI/MMT) nanocomposite films were prepared in which MMT was used as a nanofiller at 0, 3, 6, 9, 12, and 15 wt % relative to SPI dry weight. Effects of MMT on film properties including tensile strength, elongation at break, total soluble matter, water vapor permeability, and oxygen permeability were assessed. X-ray diffraction patterns were determined, and morphologies of SPI and the SPI-MMT composite films were visualized by scanning electron microscopy. Mechanical and barrier properties were improved by evidenced increases in tensile strength and modulus, and decreases in permeability to water vapor and oxygen. MMT concentrations of 3%-12% were optimal for improving functional properties of the composite films. X-ray diffraction and scanning electron microscopy examinations revealed the formation of an intercalated and exfoliated structure on the addition of MMT into the SPI matrix. We conclude that intercalated and exfoliated MMT silicates enhance mechanical and barrier properties of SPI films. © 2010 Wiley Periodicals, Inc.

Kim H.J.,Jeonnam Biofood Technology Center | Shin H.-K.,Jeonnam Biofood Technology Center | Yang E.J.,Jeonnam Biofood Technology Center
Journal of the Korean Society of Food Science and Nutrition | Year: 2013

To develop a starter culture system for the fermentation of mukeunji, we introduced lactic acid bacteria and yeast isolated from mukeunji into kimchi fermentation as a single or a mixed culture. On evaluating mukeunji flavor, we found that the mixed starter kimchi prepared with two strains, ML17 and MY7, gave the best sensory score. These strains were identified as Lactobacillus (Lb.) curvatus ML17 and Saccharomyces (S.) servazzii MY7 by molecular identification method. The fermentative characteristics of starter kimchi were investigated by measuring changes in the physicochemical and microfloral characteristics during the fermentation. The decrease in pH and increase in acidity in the starter kimchi were faster compared to respective values of control kimchi. There was a gradual decrease in hardness of starter kimchi, which was still slow compared to hardness decrease in control kimchi. Microbial analysis of starter kimchi revealed that Lb. curvatus ML17 and S. servazzii MY7 were the dominant organisms during the entire fermentation period. The lactic acid and citric acid contents of starter kimchi were higher than those of the control kimchi after 90 days of fermentation. By sensory evaluation, the starter kimchi scored higher in appearance, mukeunji flavor, sourness, carbonated flavor, savory taste, texture, and overall acceptability, but lower in off-flavor than the control kimchi. © 2013 by The Korean Society of Food Science and Nutrition. All rights reserved.

Norajit K.,Kongju National University | Kim K.M.,Jeonnam Biofood Technology Center | Ryu G.H.,Kongju National University
Journal of Food Engineering | Year: 2010

The physical and antioxidant properties of alginate biodegradable film incorporating white, red and extruded white ginseng extracts were investigated. No differences in moisture contents of all alginate film samples were noticeable (p > 0.05) except those film samples incorporated without ginseng extract, which had the highest moisture content. The addition of ginseng extract to alginate film decreased tensile strength and elastic modulus (p < 0.05) but increased the percent elongation at break. The presence of extracts did not significantly affect the water vapor permeability of the film samples. Alginate film containing extruded white ginseng extract at a barrel temperature of 130 °C presented the highest (61.12%) free-radical scavenging activity against the 2,2-diphenyl-1-picryhydrazyl radical (DPPH) in methanol, followed by film containing white ginseng extruded at a barrel temperature of 115 °C. Following these samples in terms of free-radical scavenging activity were film samples containing red and white ginseng extracts, respectively. These results revealed that extruded white ginseng extract has good potential to be incorporated into alginate to make antioxidant biodegradable film or coating for various food applications. © 2010 Elsevier Ltd. All rights reserved.

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