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Jeongeup, South Korea

Seo H.J.,Wonkwang University | Ham H.-D.,Wonkwang University | Jin H.Y.,Wonkwang University | Lee W.H.,Wonkwang University | And 9 more authors.
Korean Journal of Physiology and Pharmacology | Year: 2010

The hypothalamic-pituitary-adrenal (HPA) axis is the primary endocrine system to respond to stress. The HPA axis may be affected by increased level of corticotrophin-releasing factors under chronic stress and by chronic administration of monosodium glutamate (MSG). The purpose of this study was to investigate whether chronic MSG administration aggravates chronic variable stress (CVS)-induced behavioral and hormonal changes. Twenty-four adult male Sprague-Dawley rats, weighing 200-220 g, were divided into 4 groups as follows: water administration (CON), MSG (3 g/kg) administration (MSG), CVS, and CVS with MSG (3 g/kg) administration (CVS + MSG). In addition, for the purpose of comparing the effect on plasma corticosterone levels between chronic stress and daily care or acute stress, 2 groups were added at the end of the experiment; the 2 new groups were as follows: naïve mice (n=7) and mice exposed to restraint stress for 2 h just before decapitation (A-Str, n=7). In an open field test performed after the experiment, the CVS + MSG group significant decrease in activity. The increase in relative adrenal weights in the CVS and CVS + MSG group was significantly greater than those in the CON and/or MSG groups. In spite of the increase in the relative adrenal weight, there was a significant decrease in the plasma corticosterone levels in the CVS + MSG group as compared to all other groups, except the naïve group. These results suggest that impaired HPA axis function as well as the decrease in the behavioral activity in adult rats can be induced by chronic MSG administration under CVS rather than CVS alone.

Yhim H.Y.,Chonbuk National University | Kim H.S.,Jeonbuk Science College | Sohn J.Y.,Chonbuk National University | Song M.J.,Chonbuk National University | And 5 more authors.
Cancer Genetics and Cytogenetics | Year: 2010

Klinefelter syndrome (KS) is the most commonly diagnosed X chromosome aneuploid syndrome among males. The association between hematologic malignancies such as non-Hodgkin lymphoma and leukemia and KS has been established recently on the basis of numerous case reports and a large cohort study. The risk of chronic myeloproliferative disease (MPD) as a hematologic malignancy in KS, however, has not been evaluated to date. Moreover, to our knowledge, there is only one case report that observed MPD in a patient with KS. Even though the Janus kinase 2 (JAK2) mutation was considered the primary cause in the pathogenesis of MPD, the mutation status was not described in that report. We found JAK2 V617F mutation-positive essential thromobocythemia (ET) in a patient with KS. To the best of our knowledge, this case represents the first case report of JAK2 V617F mutation in a patient with KS. Furthermore, we also suggest the hypothesis that X chromosome aneuploidy as a " pre-JAK2" cytogenetic change may be associated with the development of clonal hematopoiesis and carcinogenesis, because of the finding of higher oncogenic potential of extra X chromosome. In this study, we present the first report of JAK2 V617F-positive ET in a patient with KS, as well as a review of the relevant literature. © 2010 Elsevier Inc.

Song M.-Y.,Chonbuk National University | Yim J.-Y.,Chonbuk National University | Yim J.-M.,Chonbuk National University | Kang I.-J.,Chonbuk National University | And 8 more authors.
Journal of Immunotherapy | Year: 2011

Nitric oxide (NO) synthesis is strongly induced during interleukin (IL)-2 treatment of mice and humans. Although this free radical can act as a cytotoxic effector molecule against cancer cells, immunosuppressive effects have also been suggested. We evaluated the effects of curcumin on IL-2-induced NO synthesis and IL-2-induced antitumor responses in a mouse ascites tumor model. Curcumin inhibited inducible nitric oxide synthase (iNOS) expression and NO production, and thereby enhanced the proliferation and cytotoxic activity of cocultured lymphocytes and macrophages during IL-2 stimulation which we earlier established as an in vitro model of IL-2-induced NO synthesis. Curcumin also decreased apoptosis of cocultured lymphocytes and macrophages during IL-2 stimulation. In contrast, the curcumin-induced changes in proliferation and apoptosis were not observed in cultures of lymphocytes alone, macrophages alone, and cocultured lymphocytes/iNOS-knock out macrophages, all of which produced little nitrite during IL-2 stimulation. In conjunction with IL-2 treatment, oral curcumin administration significantly inhibited IL-2 therapy-induced urinary nitrite/nitrate excretion and iNOS expression of tumor tissues, and further increased the IL-2 therapy-induced prolongation of survival in a murine Meth-A ascites tumor model. Curcumin may be useful as an adjunct to increase the antitumor activity of IL-2 therapy. © 2011 by Lippincott Williams & Wilkins.

Jeong I.S.,Chosun University | Choi J.Y.,Chosun University | Nho E.Y.,Chosun University | Hwang I.M.,Chosun University | And 5 more authors.
Analytical Letters | Year: 2014

Food irradiation processing needs proper control to facilitate consumer assurance, choice, and protection, including accurate detection of irradiated food. This study was aimed at analyzing hydrocarbons induced by gamma and electron beam irradiation in Camembert and processed cheeses. The samples were irradiated at 0, 1, 3, 5, 7, and 10 kGy doses of γ-rays and an electron beam. The induced hydrocarbons were separated on a florisil column and identified by gas chromatography-mass spectrometry (GC-MS). The detected hydrocarbons were pentadecane and 1-tetradecene from palmitic acid, heptadecane and 1-hexadecene from stearic acid, and 8-heptadecene and 1,7-hexadecadiene from oleic acid. The concentrations of the radiation-induced hydrocarbons increased with irradiation dose. Among the induced hydrocarbons, 1-tetradecene and 1,7-hexadecadiene were found to be the major hydrocarbons in the cheese samples. For most irradiation doses, the concentration of hydrocarbons induced by the electron beam was slightly higher than by gamma rays. These hydrocarbons were detected only in irradiated samples at 1 kGy or above, and not in the nonirradiated ones. It was concluded that the detected hydrocarbons were good markers to distinguish irradiated and nonirradiated cheese. © 2014 Copyright Taylor and Francis Group, LLC.

Jeong I.S.,Chosun University | Kim J.S.,Chosun University | Hwang I.M.,Chosun University | Choi S.H.,Chosun University | And 5 more authors.
Korean Journal of Food Science and Technology | Year: 2013

Food irradiation has recently become one of the most successful techniques to preserve food with increased shelf life. This study aims to analyze hydrocarbons in almonds {Prunus amygosalus L) and peanuts (Arachis hypogaea) induced by electron beam irradiation. The samples were irradiated at 0, 1, 3, 5 and 10 kGy by e-bcam and using florisil column chromatography fat, and content was extracted. The induced hydrocarbons were identified using gas chromatography-mass spectrometry (GC/MS). The major hydrocarbons in both irradiated samples were 1,7-hexadecadiene {C16:2) and 8-heptadecene (C17:1) from oleic acid, 1,7,10-hexadecatriene (C16:3) and 6,9-heptadecadiene (C17:2) from linoleic acid and 1-tetradecene (C14:1) and pcntadecane (C15:0) from palmitic acid. Concentrations of the hydrocarbons produced by e-beam were found to be depended upon the composition of fatty acid in both almonds and peanuts. The C n-2compound was found to be higher than Cn-1, compound in oleic acid and palmitic acid, while in case of linoleic acid, Cn-1, compound was higher than Cn-2 compound. The radiation induced hydrocarbons were detected only in irradiated samples, with 1 kGy or above, and not in the non-irradiated ones. The production of 1,7-hcxadecadicne (C 16:2), 8-hcptadecenc (C17:1), 1,7,10-hexadecatriene (C,16,3) and 6,9-hepladecadiene (C,7:2), in high concentration gave enough information to suggest that these may be the possible marker compounds of electron beam irradiation in almonds and peanuts. © The Korean Society of Food Science and Technology.

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