Lee G.,Chonnam National University |
Lee G.,Jeollanamdo Institute of Natural Resources Research |
Choi C.-Y.,Jeollanamdo Institute of Natural Resources Research |
Jun W.,Chonnam National University
Preventive Nutrition and Food Science | Year: 2016
Menopausal hot flushes (HFs), which manifest as a transient increase in skin temperature, occur most frequently in postmenopausal women, and sometimes negatively influence daily life. We investigated the effect of an aqueous extract of Cynanchum wilfordii (CWW) in a rat model of menopausal HFs, where tail skin temperature (TST) is increased after the rapid estrogen decline induced by ovariectomy. Ten-week-old female rats were ovariectomized and treated with CWW for 1 week. We measured TST and rectal temperatures (RT) and investigated serum estradiol. The TST in ovariectomized (OVX) rats was significantly elevated after ovariectomy compared with control rats, whereas the RT in OVX rats was not elevated. Administration of CWW (200 mg/kg/d for 7 days, p.o.) significantly improved the skin temperature increase in OVX rats. The lower level of serum estradiol in OVX rats was significantly increased by supplying E2, but it was not affected by CWW. The present study indicates a need for future research involving treatment with high concentrations of C. wilfordii and measurement over 24 h. Copyright © 2016 by The Korean Society of Food Science and Nutrition. All rights Reserved.
Karki R.,Mokpo National University |
Jung M.-A.,Mokpo National University |
Jung M.-A.,Jeollanamdo Institute of Natural Resources Research |
Kim K.-J.,Taekyeung College |
Kim D.-W.,Mokpo National University
Evidence-based Complementary and Alternative Medicine | Year: 2012
Atopic dermatitis (AD) is a chronic inflammatory skin disease which has a complex etiology that encompasses immunologic responses. The study was carried out to examine the effect of Nelumbo nucifera (Gaertn.) leaf (NL) on the AD-like skin lesion induced by repeated epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) on the dorsal skin of NC/Nga mice. Three different doses of NL (5, 25, and 50mg/mice/day) were administered orally from the day of sensitization with DNCB for 4 weeks. The efficacy of NL was judged by histopathological examination, blood IgE level, measurement of transepidermal water loss (TEWL), scratching behavior, and skin severity score. NL resulted in the suppression of clinical severity score, TEWL, scratching behavior, and blood IgE level. Histopathologic analyses revealed that thickening of the epidermis and mast cell degranulation was significantly reduced in NL group. These results suggest that NL may be a useful natural resource for the management of AD. Copyright 2012 Rajendra Karki et al.
Lee Y.-H.,University of Suwon |
Kwak J.,Korea University |
Choi H.-K.,Yonsei University |
Choi K.-C.,University of Seoul |
And 5 more authors.
International Journal of Molecular Medicine | Year: 2012
Manipulating acetylation status of key gene targets is likely to be crucial for effective cancer therapy. In this study, we utilized green tea catechins, epicatechin (EC), epigallocatechin (EGC) and epigallocatechin-3-gallate (EGCG) to examine the regulation of androgen receptor acetylation in androgen-dependent prostate cancer cells by histone acetyltransferase (HAT) activity. EC, EGC and EGCG induced prostate cancer cell death, suppressed agonist-dependent androgen receptor (AR) activation and AR-regulated gene transcription. These results demonstrated a similar tendency to HAT inhibitory activities; EGCG>EGC>EC. The strongest HAT inhibitor among them, EGCG (50 μM), downregulated AR acetylation and finally, AR protein translocation to nucleus from the cytoplasmic compartment was effectively inhibited in the presence of the agonist. These results suggest another mechanism to develop effective therapeutics based on green tea catechins.
Seong A.-R.,Yonsei University |
Yoo J.-Y.,Yonsei University |
Choi K.,Yonsei University |
Lee M.-H.,Yonsei University |
And 5 more authors.
Biochemical and Biophysical Research Communications | Year: 2011
Histone acetyltransferase (HAT) inhibitors (HATi) isolated from dietary compounds have been shown to suppress inflammatory signaling, which contributes to rheumatoid arthritis. Here, we identified a novel HATi in Punica granatum L. known as delphinidin (DP). DP did not affect the activity of other epigenetic enzymes (histone deacetylase, histone methyltransferase, or sirtuin1). DP specifically inhibited the HAT activities of p300/CBP. It also inhibited p65 acetylation in MH7A cells, a human rheumatoid arthritis synovial cell line. DP-induced hypoacetylation was accompanied by cytosolic accumulation of p65 and nuclear localization of IKBα. Accordingly, DP treatment inhibited TNFα-stimulated increases in NF-κB function and expression of NF-κB target genes in these cells. Importantly, DP suppressed lipopolysaccharide-induced pro-inflammatory cytokine expression in Jurkat T lymphocytes, demonstrating that HATi efficiently suppresses cytokine-mediated immune responses. Together, these results show that the HATi activity of DP counters anti-inflammatory signaling by blocking p65 acetylation and that this compound may be useful in preventing inflammatory arthritis. © 2011 Elsevier Inc.
PubMed | Jeollanamdo Institute of Natural Resources Research and Chonnam National University
Type: Journal Article | Journal: Preventive nutrition and food science | Year: 2017
Menopausal hot flushes (HFs), which manifest as a transient increase in skin temperature, occur most frequently in postmenopausal women, and sometimes negatively influence daily life. We investigated the effect of an aqueous extract of
Choi J.H.,Chungnam National University |
Jin S.W.,Chungnam National University |
Kim H.G.,Chungnam National University |
Khanal T.,Chungnam National University |
And 8 more authors.
Food and Chemical Toxicology | Year: 2013
The purpose of this study was to investigate the anti-fibrotic effects of the aqueous extract of the Platycodi Radix root (Changkil: CK) on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. DMN treatment for 4. weeks led to marked liver fibrosis as assessed by serum biochemistry, histopathological examination, and hepatic lipid peroxidation and collagen content. CK significantly inhibited DMN-induced increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, fibrosis score, and hepatic malondialdehyde and collagen content. CK also inhibited DMN-induced reductions in rat body and liver weights. Reverse transcription polymerase chain reaction (RT-PCR) and western blot analyses revealed that CK inhibited DMN-induced increases in matrix metalloproteinase-13 (MMP-13), tissue inhibitor of metalloproteinase-1 (TIMP-1), and tumor necrosis factor-α (TNF-α) mRNA, and collagen type I and α-smooth muscle actin protein. DMN-induced cyclooxygenase-2 (COX-2) expression and nuclear factor-kappa B (NF-κB) activation was reduced by CK treatment. Furthermore, CK induced activation of nuclear erythroid 2-related factor 2 (Nrf2)-mediated antioxidant enzymes such as γ-glutamylcysteine synthetase (γ-GCS), heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), and glutathione-S-transferase (GST) in HepG2 cells. These results demonstrated that CK attenuates DMN-induced liver fibrosis through the activation of Nrf2-mediated antioxidant enzymes. © 2013 Elsevier Ltd.
Park S.-Y.,Yonsei University |
Kim M.-J.,Korea University |
Kim Y.J.,Korea University |
Lee Y.-H.,University of Suwon |
And 4 more authors.
International Journal of Molecular Medicine | Year: 2015
Several recent studies have reported an association between neurodegeneration and histone modifications, such as acetylation, deacetylation and methylation. In addition, questions have been raised regarding a potential functional role for the histone acetylation enzymes in β-amyloid (Aβ)-mediated neurotoxicity, particularly the p300/CBP-associated factor (PCAF) enzyme. We recently reported the potential utility of a PCAF inhibitor in the suppression of Aβ-induced neuronal cell death, although the in vivo effectiveness of the PCAF inhibitor remained unclear. In this study, we modified the PCAF inhibitor by chemical derivatization and selected compound C-30-27 as the most potent PCAF inhibitor. We demonstrated that C-30-27 selectively inhibited acetylation-dependent nuclear factor-κB (NF-κB) at Lys-122 and suppressed the NF-κB-mediated inflammatory response induced by lipopolysaccharide (LPS) or Aβ in both BV2 and Neuro-2A (N2A) cells. Finally, we demonstrated that C-30-27 improved cognitive deficits, as well as the capacity for locomotion and the damaged cholinergic system in the Aβ-treated rats. In conclusion, our results demonstrate that this selective PCAF inhibitor has the potential to reduce the neuroinflammatory response induced by Aβ.
Yoon J.,Hanyang University |
Kim J.,Jeollanamdo Institute of Natural Resources Research |
Duy P.K.,Hanyang University |
Kim M.,Hanyang University |
And 2 more authors.
Analyst | Year: 2013
A diffuser-incorporated transmission near-infrared (NIR) scheme that enables direct spectral collection of packed granular samples with reliable sample representation and reproducibility has been demonstrated. The analytical utility of this method has been evaluated for the determination of polyethylene (PE) pellet density and the discrimination of the geographical origin of rice samples. Based on the preliminary observation of transmission spectral features acquired from spherical polyoxymethylene (POM) packings composed of different particle sizes as well as packing thickness, a portion of the radiation was propagated through the void space in the packing without fully interacting with the POM pellets. This type of radiation, so-called non-fully interacted radiation (NFIR), adversely affected the sample representation as well as the reproducibility of transmission measurements. To maximize the interaction of NIR radiation with granular samples, a polytetrafluoroethylene (PTFE) diffuser was positioned in front of the sample packing to introduce isotropically diffused radiation into the sample. This diffuser-incorporated scheme resulted in highly reproducible transmission spectra for both packed granular samples. Consequently, the density determination of PE pellets as well as discrimination of rice samples according to geographical origin was more accurate using the proposed scheme. This journal is © The Royal Society of Chemistry.
Kim S.,Jeollanamdo Institute of Natural Resources Research |
Rhim H.,Korea Institute of Science and Technology
Molecules and Cells | Year: 2011
Overload of intracellular Ca 2+ has been implicated in the pathogenesis of neuronal disorders, such as Alzheimer's disease. Various mechanisms produce abnormalities in intracellular Ca 2+ homeostasis systems. L-type Ca 2+ channels have been known to be closely involved in the mechanisms underlying the neurodegenerative properties of amyloid-β (Aβ) peptides. However, most studies of L-type Ca 2+ channels in Aβ-related mechanisms have been limited to Ca v1.2, and surprisingly little is known about the involvement of Ca v1.3 in Aβ-induced neuronal toxicity. In the present study, we examined the expression patterns of Ca v1.3 after Aβ25-35 exposure for 24 h and compared them with the expression patterns of Ca v1.2. The expression levels of Ca v1.3 were not significantly changed by Aβ25-35 at both the mRNA levels and the total protein level in cultured hippocampal neurons. However, surface protein levels of Ca v1.3 were significantly increased by Aβ25-35, but not by Aβ35-25. We next found that acute treatment with Aβ25-35 increased Ca v1.3 channel activities in HEK293 cells using whole-cell patch-clamp recordings. Furthermore, using GTP pulldown and co-immunoprecipitation assays in HEK293 cell lysates, we found that amyloid precursor protein interacts with β 3 subunits of Ca 2+ channels instead of Ca v1.2 or Ca v1.3 a1 subunits. These results show that Aβ25-35 chronically or acutely upregulates Ca v1.3 in the rat hippocampal and human kidney cells (HEK293). This suggests that Ca v1.3 has a potential role along with Ca v1.2 in the pathogenesis of Alzheimer's disease. © 2011 KSMCB.
PubMed | Jeollanamdo Institute of Natural Resources Research
Type: Journal Article | Journal: International journal of food sciences and nutrition | Year: 2013
Maesil (the fruit of Prunus mume Siebold & Zucc.) has long been used as an alternative medicine and functional food in Korea and Japan for preventive and therapeutic purposes. We examined the laxative effect of unripe Maesil (UM) and ripe Maesil (RM) in a rat model on constipation induced by a low-fibre diet and the possible mechanisms of Maesil in the rat colon. In vivo studies were conducted on the low-fibre diet-induced constipation rat model, and isolated rat colon was used in in vitro experiments to measure the changes in spontaneous colon contraction generated by Maesil and organic acids as standard and effectual ingredients, respectively. The aqueous extract of both UM and RM applied orally (100 and 300mg/kg) produced significant increase of faeces frequency (p<0.05) and moisture (p<0.001). Moreover, the number faecal pellets number was reduced (p<0.05) in the distal colons of the Maesil-treated rats. Gastrointestinal (GI) motility, measured by charcoal meal, was activated more fully by UM than in the low-fibre diet group. Both UM and RM and its organic acids produced a dose-dependent stimulation of the spontaneous contractile amplitude (p<0.001) and frequency (p<0.01) of the isolated rat colon. Although both UM and RM were an effective laxative, the RM was significantly more effective than the UM in the in vivo and in vitro constipation experiments because of the changes in the composition of organic acids during the ripening of the fruit. Our results demonstrated that Maesil was effective in promoting the frequency of defaecation and contraction of the rat colon, which provided scientific basis to support the use of Maesil as potential therapeutics in treating constipation.