Jeollanamdo Development Institute for Traditional Korean Medicine

Korean, South Korea

Jeollanamdo Development Institute for Traditional Korean Medicine

Korean, South Korea
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Lim J.-H.,Advanced Institutes of Convergence Technology | Kim T.-W.,Chungnam National University | Park S.-J.,Advanced Institutes of Convergence Technology | Song I.-B.,Chungnam National University | And 8 more authors.
Journal of Toxicologic Pathology | Year: 2011

The aim of the present study was to evaluate the protective activity of aqueous extract from Platycodon grandiflorum (BC703) on thioacetamide (TA)-induced hepatotoxicity in mice. We found that BC703 significantly decreased mortality and the change in serum transaminase following TA administration. The group treated with BC703 at doses of 1, 5, and 10 mg/kg produced significant hepatoprotective effects against TA-induced liver damage by decreasing the activities of serum enzymes, nitric oxide and lipid peroxidation in dose-dependent manners. Histopathological studies further substantiated the protective effect of BC703. These results show the hepatoprotective activity of aqueous extract from Platycodon grandiflorum on thioacetamide-induced fulminant hepatic failure. © 2011 The Japanese Society of Toxicologic Pathology.


Jeong S.Y.,Chosun University | Qian Z.-J.,Marine Life Research | Jin Y.-J.,Bio Convergence Center | Kim G.-O.,Bio Convergence Center | And 2 more authors.
Natural Product Sciences | Year: 2012

In the present work, we have collected 19 species of macroalgae (9 Phaeophta and 10 Rhodophyta) from all around of Korea: Dictyopteris divaricata, D. prolifera, Myelophycus cavus, Papenfussiella kuromo, Petalonia zosterifolia, Petrospongium rugosum, Rugulopteryx okamurae, Sargassum fulvellum, S. muticum, Callophyllis japonica, Gloiopeltis tenax, Gracilaria longissima, Gracilaria vermiculophylla, Grateloupia asiatica, Grateloupia lanceolata, Grateloupia sparsa, Grateloupia turuturu, Grateloupia sp, and Polyopes affinis. The macroalgal species were extracted by 70% ethanol (EtOH) for 24 h and evaluated its inhibitory effects on αglucosidase. Among ethanol extracts, Myelophycus cavus showed the most effectively inhibitory activity (IC 50, 2.17μg/ml) against α-glucosidase, followed by Sargassum fulvellum (IC50, 8.13 μg/ml), Dictyopteris prolifera (IC 50, 16.66 μg/ml), Rugulopteryx okamurae (IC50, 50.63 μg/ml), and Petrospongium rugosum (IC50, 101.62 μg/ ml). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay showed no cytotoxicity on mouse pre-adipocytes cell line (3T3-L1). These results suggest that some edible macroalgae merit further evaluation for clinical usefulness as anti-diabetic functional foods.


Jeong E.J.,Seoul National University | Cho J.H.,Jeollanamdo Development Institute for Traditional Korean Medicine | Sung S.H.,Seoul National University | Kim S.Y.,Kyung Hee University | Kim Y.C.,Seoul National University
Bioorganic and Medicinal Chemistry Letters | Year: 2011

The 80% methanolic extract of Euonymus alatus leaves and twigs afforded three new lignans, (-)-threo-4,9,4′,9′-tetrahydroxy-3,7,3′, 5′-tetramethoxy-8-O-8′-neolignan (1), (-)-threo-4,9,4′, 9′-tetrahydroxy-3,5,7,3′-tetramethoxy-8-O-8′-neolignan (2), (7R,8R,7′R)-(+)-lyoniresinol (3), together with seventeen known lignans (4-20). The structures of 1-20 were elucidated by extensive 1D and 2D spectroscopic methods including 1H NMR, 13C NMR, 1H-1H COSY, HMQC, HMBC and NOESY. All the isolated compounds except for dilignans (19 and 20) significantly inhibited nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells. © 2011 Elsevier Ltd. All rights reserved.


Kim T.-W.,Chungnam National University | Lim J.-H.,Advanced Institutes of Convergence Technology | Song I.-B.,Chungnam National University | Park S.-J.,Advanced Institutes of Convergence Technology | And 9 more authors.
Journal of Nutritional Science and Vitaminology | Year: 2012

The present study aims to evaluate the anti-HCV activity of hotwater extract from Platycodon grandiflorum (BC703) with HCV genotype 1b subgenomic replicon system and investigate its hepatoprotective activity on carbon tetrachloride (CCl4)-induced acute liver damage in mice. BC703 produced significant hepatoprotective effects against CCl4- induced acute hepatic injury by decreasing the activities of serum enzymes, nitric oxide and lipid peroxidation. Histopathological studies further substantiated the protective effect of BC703. Furthermore, BC703 inhibited the HCV RNA replication with an EC50 value and selective index (CC50/EC50) of 2.82 μg/mL and above 35.46, respectively. However, digested BC703 using a simulated gastric juice showed poor protective effect against CCl 4 -induced hepatotoxicity in mice and decreased anti-HCV activity as compared to the intact BC703. Although further studies are necessary, BC703 may be a beneficial agent for the management of acute hepatic injury and chronic HCV infection.


Lim J.-H.,Advanced Institutes of Convergence Technology | Kim T.-W.,Chungnam National University | Song I.-B.,Chungnam National University | Park S.-J.,Advanced Institutes of Convergence Technology | And 6 more authors.
Human and Experimental Toxicology | Year: 2013

The aim of the present study was to evaluate the protective effect of aqueous extract from Platycodon grandiflorum (BC703) on bile duct ligation (BDL)-induced hepatic fibrosis in rats. BDL rats were divided into three groups, which orally received distilled water or BC703 (10 or 50 mg/kg/day) for consecutive 28 days. Antifibrotic effects of BC703 on BDL-induced hepatic fibrosis in rats were estimated by assessing serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), blood urea nitrogen (BUN), transforming growth factor-beta 1 (TGF-β1) and hepatic levels of malondialdehyde (MDA), glutathione (GSH), total superoxide dismutase (SOD) and nitric oxide (NO). The biochemical observations were supplemented by histopathological examination of liver samples stained with hematoxylin and eosin and Masson's trichrome stain. ALT, AST, TBIL and BUN were elevated in the group treated with BDL alone than in the sham-operated group. These elevations were significantly decreased by BC703 treatment. Hepatic GSH and SOD levels, depressed by BDL, were also increased in the BC703 group. In addition, increases in hepatic MDA and NO levels in the BDL-induced cholestasis were attenuated by BC703 treatment. Furthermore, BC703 treatment significantly reduced the serum level of fibrogenic cytokine, TGF-β1. Histopathological studies further substantiated the protective effect of BC703 on BDL-induced hepatic fibrosis in rat. BC703 may have beneficial effects not only on hepatic fibrosis by cholestasis but also on hepatic fibrosis development in patients with chronic hepatic disease. © 2013 The Author(s).


Kim M.-S.,Jeollanamdo Development Institute for Traditional Korean Medicine | Park B.-K.,Chungnam National University | Hwang Y.-H.,Chungnam National University | Song I.-B.,Chungnam National University | And 5 more authors.
Journal of Poultry Science | Year: 2013

Toltrazuril is a symmetrical tiazinetrione compound. It is active against all intracellular developmental stages including those of schizogony and gametogony. In this study the disposition kinetics of toltrazuril (TZR) and its major metabolites (TZR-SO and TZR-SO2) in broiler chickens after single oral administrations of 10 or 20mg/kg were investigated. Mean plasma concentrations of TZR peaked at 16.4 and 25.2μg/mL at 5.0 and 4.7h after dosing, respectively. TZR was converted to the short-lived intermediary metabolite toltrazuril sulfoxide (TZR-SO), and then further metabolized to the reactive toltrazuril sulfone (TZR-SO2) being actually more slow eliminated with 80.3and 82.9h than TZR (10.6 and 10.7h) or TZR-SO (14.8 and 15.3h) in low and high dosing groups, respectively. Prolonged elimination half-life of TZR-SO2 could be interpreted as the persistent clinical efficacy of TZR in the treatment of protozoal parasites infection. © 2013, Japan Poultry Science Association.


Kim O.T.,National Institute of Horticultural and Herbal Science | Yoo N.H.,Chonbuk National University | Kim G.S.,South Korean National Institute of Crop Science | Kim Y.C.,South Korean National Institute of Crop Science | And 4 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2013

It has been recognized that ginsenoside Rg3 is not naturally produced in ginseng although this ginsenoside can accumulate in red ginseng as the result of a thermal process. In order to determine whether or not Rg3 is synthesized in ginseng, hairy roots were treated with methyl jasmonate (MJ). From HPLC analysis, no peak for Rg3 was observed in the controls. However, Rg3 did accumulate in hairy roots that were MJ-treated for 7 days. Rg3 content was 0.42 mg/g (dry weight). To gain more insight into the effects of MJ on UDP-glucosyltransferase (UGT) activity, we attempted to evaluate ginsenoside Rg3 biosynthesis by UGT. A new peak for putative Rg3 was observed, which was confirmed by LC-MS/MS analysis. Our findings indicate that the proteins extracted from our hairy root lines can catalyze Rg3 from Rh2. This suggests that our ginseng hairy root lines possess Rg3 biosynthesis capacity. © 2012 Springer Science+Business Media B.V.


Kim T.-W.,Chungnam National University | Song I.-B.,Chungnam National University | Lee H.-K.,Chungnam National University | Kim M.-S.,Jeollanamdo Development Institute for Traditional Korean Medicine | And 5 more authors.
Planta Medica | Year: 2013

Scutellaria baicalensis has been used as a traditional herbal medicine for bronchitis, hepatitis, and allergic diseases. The root of Scutellaria baicalensis contains active flavonoid components, including baicalin, baicalein, wogonoside, and wogonin, which have pharmaceutical properties. In the present study, the antiallergic properties of a standardized aqueous extract of S. baicalensis were evaluated, and the skin toxicity of its dermal application was also determined. The in vivo and in vitro assays were performed by using the β-hexosaminidase assay in rat basophilic leukemia cells (RBL-2H3) and cutaneous skin reaction in BALB/c mice, respectively. In addition, the acute dermal irritation/corrosion test was carried out in New Zealand white rabbits, and the skin sensitization test was conducted by Buhlers method in Hartley guinea pigs to estimate the safety of the standardized aqueous extract of S. baicalensis for topical application. β-Hexosaminidase release in RBL-2H3 was markedly decreased following treatment with the standardized aqueous extract of S. baicalensis. It also ameliorated antigen-induced ear swelling compared with the control group in BALB/c mice. In the toxicological studies, it did not induce any dermal irritation/corrosion in rabbits or skin sensitization in guinea pigs. Although still limited, these results concerning the toxicological effects of S. baicalensis could be an initial step toward the topical application of S. baicalensis extracts on hypersensitive skin. © 2013 Georg Thieme Verlag KG Stuttgart New York.

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