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Jeju, South Korea

Yang E.-J.,Jeju National University | Moon J.-Y.,Jeju National University | Kim S.S.,Jeju National University | Kim S.S.,National Institute of Horticultural and Herbal Science | And 4 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2014

Objective: To investigate the anti-inflammatory effects of Jeju seaweeds on macrophage RAW 264.7 cells under lipopolysaccharide (LPS) stimulation. Methods: Ethyl acetate fractions were prepared from five different types of Jeju seaweeds, Dictyopteris divaricata (D. divaricata), Dictyopteris prolifera (D. prolifera), Prionitis cornea (P. cornea), Grateloupia lanceolata (G. lanceolata), and Grateloupia filicina (G. filicina). They were screened for inhibitory effects on proinflammatory mediators and cytokines such as nitric oxide (NO), prostaglandin E2, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Results: Our results revealed that D. divaricata, D. prolifera, P. cornea, G. lanceolata, and G. filicina potently inhibited LPS-stimulated NO production (IC50 values were 18.0, 38.36, 38.43, 32.81 and 37.14 μg/mL, respectively). Consistent with these findings, D. divaricata, D. prolifera, P. cornea, and G. filicina also reduced the LPS-induced and prostaglandin E2 production in a concentration-dependent manner. Expectedly, they suppressed the expression of inducible NO synthase and cyclooxygenase-2 at the protein level in a dose-dependent manner in the RAW 264.7 cells, as determined by western blotting. In addition, the levels of TNF-α and IL-6, released into the medium, were also reduced by D. divaricata, D. prolifera, P. cornea, G. lanceolata, and G. filicina in a dose-dependent manner (IC50 values for TNF-α were 16.11, 28.21, 84.27, 45.52 and 74.75 μg/mL, respectively; IC50 values for IL-6 were 37.35, 80.08, 103.28, 62.53 and 84.28 μg/mL, respectively). The total phlorotannin content was measured by the Folin-Ciocalteu method and expressed as phloroglucinol equivalents. The content was 92.0 μg/mg for D. divaricata, 151.8 μg/mg for D. prolifera, 57.2 μg/mg for P. cornea, 53.0 μg/mg for G. lanceolata, and 40.2 μg/mg for G. filicina. Conclusions: Thus, these findings suggest that Jeju seaweed extracts have potential therapeutic applications for inflammatory responses. © 2014 by the Asian Pacific Journal of Tropical Biomedicine. Source


The fully controlled crop cultivation system suggested in this study is intended to develop the cultivation model for stable production of medicinal crops under enclosed environment not affected by natural settings. In this paper, the features of the fully controlled crop cultivation system for detecting optimum conditions of medicinal crop cultivation are explored and technical components to be considered upon developing the system are reviewed. © (2013) Trans Tech Publications, Switzerland. Source


The present invention relates to a composition for an inflammatory disease, using Litsea japonica, and more specifically to a composition for preventing and treating an inflammatory disease, containing the oil derived from the fruits or the seeds of Litsea japonica, and a method for preparing the same. The present invention uses the fruits (excluding the seeds) or the seeds of Litsea japonica and obtains the oil, which is used as an active ingredient of a composition for preventing and treating an inflammatory disease, by using n-hexane, wherein the oil is obtained by: separating the fruits (excluding the seeds) of Litsea japonica from the seeds; injecting n-hexane, which is a solvent, into the fruits (excluding the seeds) or the seeds, 9-11 times greater than the weight of each: stirring the same for 24 hours at room temperature and extracting the same; and removing a hexane layer.


Kim M.-J.,Jeju National University | Kim M.,Para Jeju Inc. | Lee W.J.,Jeju Technopark | Kim S.S.,National Institute of Horticultural and Herbal Science | And 2 more authors.
Biosciences Biotechnology Research Asia | Year: 2014

To investigate the suitability of the green alga Cladophora wrightiana, grown in the southern regions of the Korean Peninsula, as a source of anti-inflammatory agents for human use. Anti-inflammatory effects of the ethyl acetate extracts of C. wrightiana (CWE) were evaluated on lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. CWE were examined for their ability to inhibit pro-inflammatory mediators and cytokines, such as nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-lβ. To elucidate its inflammatory mechanisms, CWE was investigated as an inhibitor of mitogenactivated protein kinase (MAPK) in US-stimulated RAW 264.7 cells. Our results demonstrate that CWE potently inhibited LPS-induced NO production, with IC50 values of 61.25 μg/mL. Consistent with these findings, CWE reduced the LPS-induced expression of inducible NO synthase (iNOS) protein in a concentration-dependent manner, as determined using western blot analysis. Furthermore, the levels of PGE2, TNF-α, IL-6, and IL-1β released into media were reduced by CWE in a concentration-dependent manner. In addition, CWE also induced a dosedependent inhibition of the phosphorylation of mitogen activated protein kinases (MAPKs, JNK, and ERK). Collectively, the results of this study demonstrate that CWE reduces the levels of pro-inflammatory mediators and cytokines including NO, PGE2, IL-1β, TNF-α, and IL-6 via suppression of)NK and ERK phosphorylation in RAW 264.7 cells. These findings reveal, in part, the molecular basis underlying the anti-inflammatory properties of CWE. Source


Kim M.-J.,Jeju National University | Yang K.-W.,Jeju Love Co. | Lee W.J.,Jeju Technopark | Kim S.S.,National Institute of Horticultural and Herbal Science | And 2 more authors.
Journal of Applied Pharmaceutical Science | Year: 2013

Nitric oxide (NO) and prostaglandin (PG)E2, known inflammatory mediators, are critically involved in the pathogenesis of a large number of human inflammatory diseases. Therefore, a search of inducible nitric oxide synthases (iNOS) and cyclooxygenase 2 (COX-2) selective inhibitors is a useful strategy to find functional substances to alleviate inflammatory disease. In our search for anti-inflammatory ingredients, we found that extracts of Ulva fasciata (UFE) and Desmarestia viridis (DVE) inhibit the generation of NO and PGE2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. U. fasciata and D. viridis were extracted with 80% ethanol and then partitioned successively with ethyl acetate. The ethyl acetate fractions are effective dose-dependent inhibitors of LPS-induced NO and PGE2 synthesis in RAW 264.7 cells. To test the inhibitory effects of UFE and DVE on pro-inflammatory cytokines, we performed ELISA assays for tumor necrosis factor (TNF)-α, IL (interleukin)-1β, and IL(interleukin)-6 in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the UFE and DVE showed a dose-dependent decrease in the production of TNF-α, IL-1β, and IL-6. As a preliminary study of the anti-inflammatory mechanism, we determined, using the Western blot analysis, whether or not UFE and DVE inhibit the degradation of I-kappa-B-alpha (IKB-α). Our results indicate that UFE and DVE indeed prevent the degradation of IKB-α, in a dose-dependent manner. Based on these results, we suggest that extracts of U. fasciata and D. viridis be considered candidates for anti-inflammatory agents for human use. © 2013 Min-Jin Kim et al. Source

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