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Chennai, India

Rathanavel C.,Tamil University | Sumitha J.,JBAS College for Women | Thillai Arasu P.,Kalasalingam University
International Journal of Pharma and Bio Sciences | Year: 2014

The use of medicinal plants in the treatment of respiratory illness, dermatophytes and infectious diseases is common in traditional medicine. t. Therefore, in the present investigation the antimicrobial activity was evaluated in different parts of seven selected medicinal plant extracts such as seeds of Citrullus colocynthis, Mucuna prurita, Strychno potatorum, Strychnosnux-vomica, Roots of Glycyrrhiza glabra, Plumbago zeylanica and Salacia oblonga. The extracts were obtained using methanol, ethylacetate and chloroform. The antimicrobial potential of 21 extracts collected using three different solvents were subjected to screening against five strains of the bacteria species, Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Proteus mirabilis using disc diffusion method. Broad-spectrum antibiotics, Ampicillin, Cephalotaxine and Gentamicin were used as control drugs. The plant extracts showed more activity that is significant against Gram-positive bacteria than Gram-negative bacteria. Among the three solvent extracts, methanol extract have higher activity against bacteria. Ethyl acetate and chloroform extracts indicates moderate inhibition zone in medicinal plant extracts. This result showed that the plant extracts inhibited bacterial growth but their effectiveness is varied.

Sherin Percy Prema Leela J.,JBAS College for Women | Hemamalini R.,Queen Marys College | Muthu S.,Sri Venkateswara College of Engineering | Al-Saadi A.A.,King Fahd University of Petroleum and Minerals
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2015

Capsicum a hill grown vegetable is also known as red pepper or chili pepper. Capsaicin(8-Methyl-N-vanillyl-6-nonenamide) is the active component in chili peppers, which is currently used in the treatment of osteoarthritis, psoriasis and cancer. Fourier transform infrared (FT-IR) spectrum of Capsaicin in the solid phase were recorded in the region 4000-400 cm-1 and analyzed. The vibrational frequencies of the title compound were obtained theoretically by DFT/B3LYP calculations employing the standard 6-311++G(d,p) basis set and were compared with Fourier transform infrared spectrum. Complete vibrational assignment analysis and correlation of the fundamental modes for the title compound were carried out. The vibrational harmonic frequencies were scaled using scale factor, yielding a good agreement between the experimentally recorded and the theoretically calculated values. Stability of the molecule arising from hyper conjugative interactions, charge delocalization and intra molecular hydrogen bond-like weak interaction has been analyzed using Natural bond orbital (NBO) analysis by using B3LYP/6-311++G(d,p) method. The results show that electron density (ED) in the σ∗ and π∗ antibonding orbitals and second-order delocalization energies E (2) confirm the occurrence of intra molecular charge transfer (ICT) within the molecule. The dipole moment (μ), polarizability (α) and the hyperpolarizability (β) values of the molecule has been computed. Thermodynamic properties (heat capacity, entropy and enthalpy) of the title compound at different temperatures were calculated. © 2015 Elsevier B.V.

Sumitha J.,Bharathiar University | Sumitha J.,JBAS College for Women
International Journal of Pharma and Bio Sciences | Year: 2014

The study of caffeine degrading plasmid from Brevibacterium, isolated and maintained in our laboratory from the coffee samples obtained from West Karnataka was undertaken.A plasmid of about 2000 bp was isolated from Brevibacterium . The isolated plasmid was used to transform Escherichia coli DH5α and the transformed colonies were inoculated in 1 to 10 g/L of caffeine containing minimal media to investigate whether the plasmid was involved in biodegradation of caffeine. It was observed that the plasmid biodegraded caffeine up to 8 g/L in minimal media, whereas non-transformed colonies could tolerate only up to 1 g/L caffeine. Growth curves obtained in the minimal media showed that transformed cells of Escherichia coli DH5α have greater ability to tolerate and degrade caffeine as compared to non-transformed cells.

Jasmine Shahina S.K.,University of Madras | Jasmine Shahina S.K.,JBAS College for Women | Krishnan P.,University of Madras
International Journal of Phytomedicine | Year: 2013

Asymptomatically colonized healthcare workers are the major source of Methicillin-Resistant Staphylococcus aureus (MRSA) in the hospital environment and serve as links in the transmission of MRSA among patients. Honey is known for antimicrobial properties due to various factors including phytochemical components eg: methyl glyoxal (MGO). Hence, the present study was designed to compare the antimicrobial activity of Manuka honey with native honey against methicillin resistant staphylococci from asymptomatic health care workers. Nasal swabs were collected from anterior nares of 100 healthcare workers from the hospital set up and a total of 36 isolates of staphylococci (36%) were obtained. The study has shown 7% carriage rate of MRSA and 9% MRCoNS among the healthcare workers. Initial screening with agar well diffusion method with Manuka honey showed higher inhibitory activity against all the methicillin resistant staphylococcal isolates giving a zone size of 30 mm at 50%(v/v) compared to native honey which gave zone size of 12 mm for 50% (v/v). Lower MIC of methicillin resistant staphylococci was seen for Manuka honey (6.3% to 12.5%), while the MIC of the native honey was found to be 50% in our study. The present study shows higher efficacy of Manuka honey compared to native honey against both MRSA and MRCoNS. Manuka honey shows promise as an antibacterial agent for methicillin resistant staphylococcus.

Mubeen Sultana D.,JBAS College for Women | Dawood N.,JBAS College for Women
Pollution Research | Year: 2011

Chromium, though a nonessential element in our diet has been found to possess carcinogenic effects. Since Cr(VI) is considered more toxic than the naturally occurring Cr(III), potassium chromate was chosen for this study. The short term immunological effects of injecting potassium chromate, human A antigen and trinity root extract both singly and in different combinations in rabbits (Oryctolagus cunniculus) were studied and compared with normal controls. Heamagglutination profiles, Total WBC counts and Differential WBC counts were found to fluctuate in all groups of animals. The study also proves the immune boosting action of trinity roots. Copyright © EM International.

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