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Wickneswari R.,National University of Malaysia | Bhuiyan M.A.R.,National University of Malaysia | Kalluvettankuzhy S.K.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Lim L.S.,National University of Malaysia | And 3 more authors.
Plant Molecular Biology Reporter | Year: 2012

A backcross breeding strategy was used to identify quantitative trait loci (QTLs) associated with 14 traits in a BC 2F 2 population derived from a cross between MR219, an indica rice cultivar and an accession of Oryza rufipogon (IRGC 105491). A total of 261 lines were genotyped with 96 microsatellite markers and evaluated for plant morphology, yield components and growth period. The genetic linkage map generated for this population with an average interval size of 16. 2 cM, spanning 1,553. 4 cM (Kosambi) of the rice genome. Thirty-eight QTLs were identified with composite interval mapping (CIM), whereas simple interval mapping (SIM) resulted in 47 QTLs (LOD >3. 0). The O. rufipogon allele was favourable for 59% of QTLs detected through CIM. Of 261 BC 2F 2 families, 26 advanced backcross breeding lines (BC 2F 5) were used for QTL validation. These lines were selected on the basis of the yield traits potentiality in BC 2F 3 and BC 2F 4 generations. The field trial was conducted at three different locations in Malaysia using randomized complete block design with three replications. Trait based marker analysis was done for QTL determination. Twenty-five QTLs were detected in BC 2F 5 generation whereas 29 QTLs were detected in BC 2F 2 generation of the same population. Two QTLs (qPL-1 and qSPL-7) were not considered for validation due to their low R 2 values and two QTLs (qPSS-3-2 and qGW-3-2) were not detected in the BC 2F 5 population. Fifteen QTLs showed the beneficial effect to enhance the trait value of the breeding lines. QTL validation aided to select the promising lines for further utilization. © 2012 Springer-Verlag.


Shine V.J.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Latha P.G.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Suja S.N.R.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Anuja G.I.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | And 2 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2014

Objective: To evaluate the hepatoprotective and antioxidant properties of alkaloid extract of Cyclea peltata (C. peltata) against paracetamol/carbon tetra chloride induced liver damage in Wistar rats. Methods: In vivo paracetamol/carbon tetrachloride induced liver damage in Wistar rats, in vitro free radical scavenging studies, HPTLC estimation of tetrandrine and direct analysis in real time- mass spectrometry of alkaloid extract of C. peltata were used for the validation. Results: The results showed that pretreatment with alkaloid extract of C. peltata caused significant reduction of serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase, serum alkaline phosphatase, serum cholesterol, liver malondialdehyde levels. The reduced glutathione, catalase, superoxide dismutase levels in liver were increased with alkaloid extract of C. peltata treatment. These results were almost comparable to silymarin and normal control. Histopathological studies also substantiated the biochemical findings. The in vitro hydroxyl, superoxide and DPPH scavenging study of alkaloid extract of C. peltata showed significant free radical scavenging property. Conclusions: The hepatoprotective property of alkaloid extract of C. peltata against paracetamol/carbon tetrachloride may be due the synergistic action of alkaloids especially tetrandrine, fangchinoline through free radical scavenging and thus preventing oxidative stress. © 2014 by the Asian Pacific Journal of Tropical Biomedicine.


Geethakumary M.P.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Pandurangan A.G.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Santhosh Kumar E.S.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI
Rheedea | Year: 2012

The present collection of Cinnamomum litseaefolium Thwaites in southern Western Ghats forms a new distributional record for India since, it was thought to be endemic to Sri Lanka. An illustrated account with relevant notes is provided herewith for further studies on this species.


Nadiya F.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Anjali N.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI | Gangaprasad A.,Kerala University | Sabu K.K.,Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI
Analytical Biochemistry | Year: 2015

Abstract Due to the presence of a diverse array of metabolites, no standard method of RNA isolation is available for plants. We noted that polysaccharide and polyphenol contents of cardamom tissues critically hinder the RNA extraction procedure. Hence, we attempted several methods for obtaining intact mRNA and small RNA from various cardamom tissues. It was found that protocols involving a combination of commercial kits and conventional CTAB (cetyl trimethylammonium bromide) methods yielded RNA with good purity, higher yield, and good integrity. The total RNA isolated through this approach was found to be amenable for transcriptome and small RNA analysis through next-generation sequencing platforms. © 2015 Elsevier Inc.


Salin Raj P.,Indian National Institute for Interdisciplinary Science and Technology | Prathapan A.,Indian National Institute for Interdisciplinary Science and Technology | Sebastian J.,Indian National Institute for Interdisciplinary Science and Technology | Antony A.K.,Indian National Institute for Interdisciplinary Science and Technology | And 5 more authors.
Natural Product Research | Year: 2014

This study evaluated the inhibitory potential of ethyl acetate extract of Parmotrema tinctorum (PTEE), an edible lichen, against aldose reductase (AR) and carbohydrate digestive enzymes such as α-glucosidase and α-amylase. It was also screened for antioxidant activities by using DPPH, ABTS, superoxide and hydroxyl radical-scavenging assays. PTEE exhibited α-glucosidase, α-amylase and AR inhibition along with significant antiglycation potential with an estimated IC50 value of 58.45 ± 1.24, 587.74 ± 3.27, 139.28 ± 2.6 and 285.78 ± 1.287 g/mL, respectively. Antioxidant activity of PTEE against DPPH (IC50 396.83 ± 2.98 g/mL), ABTS (151.34 ± 1.79 g/mL), superoxide (30.29 ± 1.17 g/mL) and hydroxyl (35.42 ± 1.22 g/mL) radicals suggests the antioxidant potential of P. tinctorum. Significant antioxidant activity and inhibitory potential against carbohydrate digestive enzymes and AR suggest that P. tinctorum can be developed as functional food/nutraceuticals for diabetes after detailed study. © 2014 Taylor & Francis.


PubMed | Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI
Type: Journal Article | Journal: SpringerPlus | Year: 2016

The main goal of the work was to analyse intraspecific variation in Elettaria cardamomum Maton (cardamom) using genome size, cytological studies and molecular marker data. Nuclear DNA content and molecular marker details furnish data on genome size and genetic diversity respectively among the studied accessions and both complement each other for evolutionary and taxonomic studies.The relative 2C genome size and total number of base pairs of cardamom was determined through flow cytometric analysis using propidium iodide staining. The nuclear DNA content was estimated in various sections of the species representing individuals from wild and cultivar genotypes following Zea mays L. CE-777 (2C=5.43pg) as internal reference standard. Chromosome number from growing root tip was examined following standard protocols. Twenty-six ISSR primers that generated polymorphic bands were used for genetic diversity analysis of the thirty accessions of cardamom. Estimated nuclear 2C DNA content ranged from 2.57 to 3.22pg demonstrating 1.25-fold variation. The mean amount of 2C nuclear DNA of the cardamom was calculated as 2.87pg which is equivalent of 2806 Mbp as the diploid genome size. The chromosome number was found to be 2n=48. Among the thirty accessions of cardamom studied using ISSR markers, C53 (feral from Bonacaud) showed a very prominent level of genetic diversity and was lowest for C96 (Avinash-I, a released variety from Indian Institute of Spices Research, Kozhikode).These analyses revealed the existence of genetic variability within the studied cardamom accessions. The plant specimens also differed significantly in their genome size. However, the genetic variability parameters did not show any correlation with genome size.


PubMed | Sree Narayana College and Jawaharlal Nehru Tropical Botanic Garden and Research Institute JNTBGRI
Type: Journal Article | Journal: Asian Pacific journal of tropical biomedicine | Year: 2014

To evaluate the hepatoprotective and antioxidant properties of alkaloid extract of Cyclea peltata (C. peltata) against paracetamol/carbon tetra chloride induced liver damage in Wistar rats.In vivo paracetamol/carbon tetrachloride induced liver damage in Wistar rats, in vitro free radical scavenging studies, HPTLC estimation of tetrandrine and direct analysis in real time- mass spectrometry of alkaloid extract of C. peltata were used for the validation.The results showed that pretreatment with alkaloid extract of C. peltata caused significant reduction of serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase, serum alkaline phosphatase, serum cholesterol, liver malondialdehyde levels. The reduced glutathione, catalase, superoxide dismutase levels in liver were increased with alkaloid extract of C. peltata treatment. These results were almost comparable to silymarin and normal control. Histopathological studies also substantiated the biochemical findings. The in vitro hydroxyl, superoxide and DPPH scavenging study of alkaloid extract of C. peltata showed significant free radical scavenging property.The hepatoprotective property of alkaloid extract of C. peltata against paracetamol/carbon tetrachloride may be due the synergistic action of alkaloids especially tetrandrine, fangchinoline through free radical scavenging and thus preventing oxidative stress.

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