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Kakogawa, Japan

Saotome T.,Tokyo University of Agriculture and Technology | Saotome T.,Japan Wool Textile Co. | Uesugi S.,Japan Wool Textile Co. | Kinugasa A.,Japan Wool Textile Co. | And 3 more authors.
Sen'i Gakkaishi | Year: 2014

The small-diameter (less than 6.0 mm in diameter) vascular grafts are highly desired due to a large demand of surgical revascularization. In this study, a small-diameter (6mm) vascular graft was prepared with braided Bombyx mori silk fiber tube coated by B. mori silk fibroin aqueous solution, with the goal of achieving sufficient physical strength and good patency of long term. The mechanical properties were evaluated by circumferential tensile test and suture strength test, indicating that the properties were improved remarkably by silk coating. The braiding silk fibroin graft was also evaluated by implantation into the dog abdominal aorta. The color doppler sonography after implantation for 11 months showed that there are neither dilatation nor constriction. At 2 years after implantation of the grafts in dog abdominal aorta, the graft patency and endothelial cell lining of the lumen surfaces were observed. These results demonstrate the feasibility of using braiding silk fibroin vascular graft as a small diameter vascular graft with long term patency.

Saotome T.,Tokyo University of Agriculture and Technology | Saotome T.,Japan Wool Textile Co. | Hayashi H.,Tokyo University of Agriculture and Technology | Tanaka R.,Tokyo University of Agriculture and Technology | And 6 more authors.
Journal of Materials Chemistry B | Year: 2015

Bombyx mori silk fibroin (SF) was successfully used for vascular grafts implanted in rats or dogs. Current transgenic technology can be developed to produce SF with improved properties. In this study, the vascular endothelial growth factor (VEGF) or the repeated fibronectin-derived sequence, TGRGDSPAS, and arginylglycylaspartic acid (RGD) were introduced into the SF heavy chain to improve its properties. A blood compatibility assay was performed to study lactose dehydrogenase (LDH) activity for both transgenic and wild type SF. Growth of human umbilical endothelial cells (HUVECs) showed greater enhancement of cellularization behaviour for the transgenic SF samples (VEGF and RGD) than for the wild type (WT) SF. VEGF SF also showed lower platelet adhesion than the RGD SF and WT SF. An in vivo implantation study supported these in vitro results. In particular, early endothelialisation was observed for VEGF transgenic SF, including the occurrence of native tissue organization at three months after implantation in rat abdominal aorta. © The Royal Society of Chemistry 2015.

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