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Bunkyo-ku, Japan

Japan Women's University is the oldest and largest of private Japanese women's universities. The university was established in 20 April 1901 by education reformist Jinzo Naruse Wikipedia.

Hayashi H.,Japan Womens University
X-Ray Spectrometry | Year: 2011

We have developed a program for simulating resonant inelastic X-ray scattering (RIXS), SIM-RIXS that can be run on personal computers with a Microsoft Windows XP/Vista operating system. RIXS primarily reflects lifetime-broadening-suppressed (LBS) or lifetime-broadening-suppressed state-selective (LBSS) X-ray absorption fine structure (XAFS). SIM-RIXS can convert theoretical LBS/LBSS-XAFS profiles to RIXS spectra, and it can derive an optimal LBS/LBSS-XAFS profile to reproduce experimental RIXS spectra. SIM-RIXS has three basic modules, and each user-friendly graphical user interface enables even novices, who are unfamiliar with RIXS analysis, to use this software. In this software, the first module sets conditions using conventional XAFS data; the second module does the same for conventional X-ray fluorescence data; and the third module is used for RIXS simulation based on the Tulkki-Åberg equation. An example of its application to the EuLγ4 RIXS of Eu2O3 is presented. SIM-RIXS can readily provide a basic description of the excitation and emission energy dependence of EuLγ4 RIXS, elucidating the existence of the 'hidden' peak at 8056 eV in the Eu(III) L1-XAFS. This demonstrates the usefulness of SIM-RIXS in RIXS analyses. © 2011 John Wiley & Sons, Ltd. Source

Yoshida T.,Kyoto Sangyo University | Sugano Y.,Japan Womens University
Archives of Biochemistry and Biophysics | Year: 2015

Dye-decolorizing peroxidase from the basidiomycete Bjerkandera adusta Dec 1 (DyP) is a heme peroxidase. This name reflects its ability to degrade several anthraquinone dyes. The substrate specificity, the amino acid sequence, and the tertiary structure of DyP are different from those of the other heme peroxidase (super)families. Therefore, many proteins showing the similar amino acid sequences to that of DyP are called DyP-type peroxidase which is a new family of heme peroxidase identified in 2007. In fact, all structures of this family show a similar structure fold. However, this family includes many proteins whose amino acid sequence identity to DyP is lower than 15% and/or whose catalytic efficiency (kcat/Km) is a few orders of magnitude less than that of DyP. A protein showing an activity different from peroxidase activity (dechelatase activity) has been also reported. In addition, the precise physiological roles of DyP-type peroxidases are unknown. These facts raise a question of whether calling this family DyP-type peroxidase is suitable. Here, we review the differences and similarities of structure and function among this family and propose the reasonable new classification of DyP-type peroxidase family, that is, class P, I and V. In this contribution, we discuss the adequacy of this family name. © 2015 Elsevier Inc. All rights reserved. Source

Van C.,Stanford University | Yan S.,Harvard University | Michael W.M.,Harvard University | Waga S.,Japan Womens University | Cimprich K.A.,Stanford University
Journal of Cell Biology | Year: 2010

Stalled replication forks activate and are stabilized by the ATR (ataxia-telangiectasia mutated and Rad3 related)-mediated checkpoint, but ultimately, they must also recover from the arrest. Although primed single-stranded DNA (ssDNA) is sufficient for checkpoint activation, it is still unknown how this signal is generated at a stalled replication fork. Furthermore, it is not clear how recovery and fork restart occur in higher eukaryotes. Using Xenopus laevis egg extracts, we show that DNA replication continues at a stalled fork through the synthesis and elongation of new primers independent of the checkpoint. This synthesis is dependent on the activity of proliferating cell nuclear antigen, Pol-δ, and Pol-ε, and it contributes to the phosphorylation of Chk1. We also used defined DNA structures to show that for a fixed amount of ssDNA, increasing the number of primer-template junctions strongly enhances Chk1 phosphorylation. These results suggest that new primers are synthesized at stalled replication forks by the leading and lagging strand polymerases and that accumulation of these primers may contribute to checkpoint activation. © 2010 Van et al. Source

Nagai R.,Japan Womens University | Murray D.B.,University of Mississippi | Metz T.O.,Pacific Northwest National Laboratory | Baynes J.W.,University of South Carolina
Diabetes | Year: 2012

This article outlines evidence that advanced glycation end product (AGE) inhibitors and breakers act primarily as chelators, inhibiting metal-catalyzed oxidation reactions that catalyze AGE formation. We then present evidence that chelation is the most likely mechanism by which ACE inhibitors, angiotensin receptor blockers, and aldose reductase inhibitors inhibit AGE formation in diabetes. Finally, we note several recent studies demonstrating therapeutic benefits of chelators for diabetic cardiovascular and renal disease. We conclude that chronic, low-dose chelation therapy deserves serious consideration as a clinical tool for prevention and treatment of diabetes complications. © 2012 by the American Diabetes Association. Source

Sasaki N.,Japan Womens University
Analytical Sciences | Year: 2012

AC electrokinetics is a generic term that refers to an induced motion of particles and fluids under nonuniform AC electric fields. The AC electric fields are formed by application of AC voltages to microelectrodes, which can be easily integrated into microfluidic devices by standard microfabrication techniques. Moreover, the magnitude of the motion is large enough to control the mass transfer on the devices. These advantages are attractive for biomolecular analysis on the microfluidic devices, in which the characteristics of small space and microfluidics have been mainly employed. In this review, I describe recent applications of AC electrokinetics in biomolecular analysis on microfluidic devices. The applications include fluid pumping and mixing by AC electrokinetic flow, and manipulation of biomolecules such as DNA and proteins by various AC electrokinetic techniques. Future prospects for highly functional biomolecular analysis on microfluidic devices with the aid of AC electrokinetics are also discussed. © 2012 The Japan Society for Analytical Chemistry. Source

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