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Murakami R.,Japan Food Hygiene Association | Mutsuga M.,Japan National Institute of Health Sciences | Abe T.,Japan Food Research Laboratories | Abe Y.,Japan National Institute of Health Sciences | And 21 more authors.
Journal of the Food Hygienic Society of Japan | Year: 2015

An interlaboratory study was performed to evaluate a migration test method of antimony (Sb) and germanium (Ge), based on the Japanese Food Sanitation Law for food- contact polyethylene terephthalate. Eighteen laboratories participated, and quantified Sb and Ge in three test solutions as blind duplicates using graphite furnace atomic absorption spectrometry (GF-AAS), inductively coupled plasma-optical emission spectrometry (ICP-OES) or induced coupled plasma-mass spectrometry (ICP-MS). Statistical analysis revealed that the trueness, repeatability and reproducibility were 98-107%, 1.7-7.5% and 2.0-18.8% by using GF-AAS and ICP-OES. The performance of these methods is sufficient for testing the specifications. The performance parameters of ICP-MS were 99-106%, 0.7-2.2% and 2.2-10.5%, respectively. ICP-MS is available as an alternative measuring method. However, in some laboratories, the quantitative values of Sb were higher than the addition levels. We found that Sb in working solutions is absorbed on glass vessels. Careful control of concentration in working solutions is required for Sb analysis. © 2015, Food Hygienic Society of Japan. All rights reserved.


Shibata H.,Toyomi Center | Mutsuga M.,Japan National Institute of Health Sciences | Abe Y.,Japan National Institute of Health Sciences | Itou S.,Japan Food Hygiene Association | And 21 more authors.
Journal of the Food Hygienic Society of Japan | Year: 2015

Using six kinds of zinc solution in water and 4% acetic acid as samples, an interlaboratory study was performed to evaluate a zinc (Zn) test method for food-contact rubber products, based on the Japanese Food Sanitation Law. Eighteen laboratories participated, and quantified Zn in six test solutions as blind duplicates using flame atomic absorption spectrometry, induced coupled plasmaoptical emission spectrometry or induced coupled plasma-mass spectrometry. Statistical analysis revealed that the trueness, repeatability (RSDr) and reproducibility (RSDr) were 97-103%, 0.7- 4.9% and 1.7-8.9% by all measuring methods. The values of the performance parameter fulfilled the target value (trueness: 80-110%, RSDr: 10%, RSDr: 25%). The performance of these methods is sufficient for testing the adherence of samples to the specifications.


Mutsuga M.,Japan National Institute of Health Sciences | Abe T.,Japan Frozen Foods Inspection Corporation | Abe Y.,Japan National Institute of Health Sciences | Ishii R.,Saitama Prefectural Institute of Public Health | And 19 more authors.
Journal of the Food Hygienic Society of Japan | Year: 2014

An interlaboratory study was performed to evaluate a migration test method of cadmium (Cd) and lead (Pb), based on the Japanese Food Sanitation Law for glassware, ceramicware, enamelware and metal cans. Seventeen laboratories participated, and quantified Cd and Pb in eight test solutions as blind duplicates using flame atomic absorption spectrometry (AAS), graphite furnace atomic absorption spectrometry (GF-AAS), inductively coupled plasma-optical emission spectrometry (ICP-OES) or induced coupled plasma-mass spectrometry (ICP-MS). Statistical analysis revealed that the trueness, repeatability (RSDr) and reproducibility (RSDr) were 93-105%, 0.7-8.4% and 2.6-19.3% by using AAS, ICP-OES and ICP-MS (internal standard method). The performance of these methods is sufficient for testing specifications. However, some of the RSDr values exceeded 10% in GF-AAS, and careful control of accuracy is required.


Sonobe H.,Japan Recreation and Miscellaneous Goods Safety Laboratory | Mutsuga M.,Japan National Institute of Health Sciences | Abe T.,Japan Food Research Laboratories | Abe T.,Japan Food Hygiene Association | And 26 more authors.
Journal of the Food Hygienic Society of Japan | Year: 2016

Using polystyrene, acrylonitrile-styrene resin and acrylonitrile-butadiene-styrene resin pellets as samples, an interlaboratory study was performed to evaluate the volatiles test method, based on the specifications described in the Japanese Food Sanitation Law for food-contacting polystyrene products. The study was conducted with the participation of twenty-one laboratories. Each laboratory quantified the contents of styrene, toluene, ethylbenzene, isopropylbenzene and propylbenzene in three test pellets using GC-FID, GC-MS or headspace-GC-FID. Statistical analysis revealed that the repeatability (RSDr) and reproducibility (RSDr) were 1.0-2.6 and 2.5-5.5% for the GC-FID method. The values of the performance parameters fulfilled the requirements (RSDr: 10), RSDr: 25%), and the performance is sufficient for specifications testing. The RSDr and RSDr of results obtained using the GC-MS and HS-GC methods were 1.4-7.8 and 4.9-13% (GC-MS), and 2.0-2.6 and 3.3-6.9%(HS-GC-FID), respectively. The quantified levels were similar to those obtained with GC-FID. The study suggests that the GC-MS and HS-GC methods can be employed as alternative methods to the GC-FID method.


Mano J.,Japan National Agriculture and Food Research Organization | Harada M.,Japan National Agriculture and Food Research Organization | Takabatake R.,Japan National Agriculture and Food Research Organization | Furui S.,Japan National Agriculture and Food Research Organization | And 9 more authors.
Journal of AOAC International | Year: 2012

We have developed a real-time PCR array method to comprehensively detect genetically modified (GM) organisms. In the method, genomic DNA extracted from an agricultural product is analyzed using various qualitative real-time PCR assays on a 96-well PCR plate, targeting for individual GM events, recombinant DNA (r-DNA) segments, taxon-specific DNAs, and donor organisms of the respective r-DNAs. In this article, we report the single-laboratory validation of both DNA extraction methods and component PCR assays constituting the real-time PCR array. We selected some DNA extraction methods for specified plant matrixes, i.e., maize flour, soybean flour, and ground canola seeds, then evaluated the DNA quantity, DNA fragmentation, and PCR inhibition of the resultant DNA extracts. For the component PCR assays, we evaluated the specificity and LOD. All DNA extraction methods and component PCR assays satisfied the criteria set on the basis of previous reports. © 2012 Publishing Technology.


Ohtsuki T.,Japan National Institute of Health Sciences | Kawasaki Y.,Japan National Institute of Health Sciences | Kubota H.,Japan National Institute of Health Sciences | Namiki T.,Japan Inspection Association of Food and Food Industry Environment | And 9 more authors.
Journal of the Food Hygienic Society of Japan | Year: 2011

Carbon monoxide (CO) treatment of fish meat of tuna, yellowtail, tilapia etc. is not allowed in Japan, since it can maintain the red color for a longer period than the microbiological shelf life of fish meat. The offcial method for quantification of CO has a problem, in that a part of the CO is lost during the preparation of the fish sample. To solve this problem, we modified the offcial method in this study. We also applied this modified method to survey the contents of CO in tuna, yellowtail, young yellowtail, and tilapia. As a result, the modified method was found to be more suitable for CO quantification than the offcial method. An inter-laboratory study by 4 laboratories confirmed that the CO content of many samples of tilapia exceeded the regulation value, apparently due to the higher recovery of CO, compared to the offcial method. Therefore, it was suggested that the regulation value in the case of tilapia should be changed if this method is introduced as an offcial method.


Matsumoto T.,National Institute of Health and Nutrition | Suzuki H.,National Institute of Health and Nutrition | Takebayashi J.,National Institute of Health and Nutrition | Murakami T.,Japan Institute for Environmental Sciences | And 7 more authors.
Bunseki Kagaku | Year: 2012

Currently, approval and license examinations of Food for Special Dietary Uses (FOSDU) have been held by six testing institutions. For proper operation of the FOSDU system, it is important to maintain the analytical accuracy, however, the accuracy of analysis among the groups has never been discussed. For that reason, we carried out a study to clarify the factors affecting the interlaboratory analytical accuracy with vitamin B12 contents in fortified food. As a result, it was found to be important that it has to adhere closely to the analytical method for the assurance of reproducibility, and has to use the most suitable approximate method against the calibration curve and/or to take account the proliferation potential of strain for improvement of reproducibility in the microbiological assay for the vitamin B12 quantitation. © 2012 The Japan Society for Analytical Chemistry.


Kawamura Y.,Japan National Institute of Health Sciences | Etoh M.,Japan Inspection Association of Food and Food Industry Environment | Hirakawa Y.,Japan Inspection Association of Food and Food Industry Environment | Abe Y.,Japan National Institute of Health Sciences | Mutsuga M.,Japan National Institute of Health Sciences
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2014

Bisphenol A (BPA) concentrations were surveyed in 100 domestic and 60 imported canned foods purchased from the Japanese market in 2011-12. BPA was extracted from the canned foods, derivatised by ethylation and analysed using GC-MS. In the domestic canned foods, the maximum and average BPA concentrations were 30 and 3.4 ng g-1, respectively, while in the imported canned foods they were 390 and 57 ng g-1, respectively. The BPA level in the domestic canned foods was significantly lower than that in the imported canned foods. Based on these results, the intakes of BPA from the domestic and imported canned foods in Japan were estimated as 644 ng person-1 day-1. The Japanese BPA intake was the second lowest following New Zealand, although imported canned foods increased. It was sufficiently lower than the tolerable daily intake of EFSA and the USEPA. The drastic reduction of BPA in the domestic canned foods should be due to the 'BPA reduced cans' that Japanese can manufacturers had developed in the late 1990s and became widely used in Japan. © 2014 © 2014 Taylor & Francis.


Minematsu K.,Japan Inspection Association of Food and Food Industry Environment | Nakamurah K.,Japan National Institute of Health Sciences | Akiyama H.,Japan National Institute of Health Sciences | Harikai N.,Mukogawa Women's University | And 4 more authors.
Journal of the Food Hygienic Society of Japan | Year: 2010

Rice powder containing Konjak flour made with tuberous roots of Amorphophallus konjac is imported as a rice-processed product from China to Japan. An improved DNA purification method for the polymerase chain reaction (PCR) analysis of rice in such products is necessary, since Konjak flour constituents absorb the DNA purification buffer to form a gel, and cause problems in the subsequent purification steps. Here, we present a simple preparative system for isolation of the rice and a purification method of the rice DNA from the product. The purified DNA was confirmed to be a good template for both PCR and real-time PCR.

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