Japan Bio. Science Laboratory Co.

Ibaraki, Japan

Japan Bio. Science Laboratory Co.

Ibaraki, Japan
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Ohkura N.,Teikyo University | Nakakuki Y.,Teikyo University | Taniguchi M.,Osaka University of Pharmaceutical Sciences | Kanai S.,Teikyo University | And 7 more authors.
BioFactors | Year: 2011

The folk medicine Angelica keiskei (Ashitaba) exhibits antitumor, antioxidant and antidiabetic activities and it has recently attracted attention as a health food. Ashitaba is thought to have antithrombotic properties, but this has not yet been scientifically proven. The elevation of plasma plasminogen activator inhibitor 1 (PAI-1), an inhibitor of fibrinolysis results in a predisposition to the risk of thrombosis. The present study showed that Ashitaba exudates injected intraperitoneally and orally administered over long-term suppressed the lipopolysaccharide (LPS) induced PAI-1 increase in mouse plasma. We also found that xanthoangelol, xanthoangelols B and D, the components of Ashitaba exudates, significantly inhibited TNFα-induced PAI-1 production from human umbilical vein endothelial cells (HUVECs). These findings suggest that Ashitaba can decrease elevated PAI-1 production, and that daily consumption of Ashitaba product might maintain anticoagulant status by inhibiting elevations in PAI-1 under inflammatory conditions. © 2011 International Union of Biochemistry and Molecular Biology, Inc.


Fujita M.,Hiroshima International University | Ohnishi K.,Japan Bio. Science Laboratory Co. | Takaoka S.,Japan Bio. Science Laboratory Co. | Ogasawara K.,Japan Bio. Science Laboratory Co. | And 2 more authors.
Biological and Pharmaceutical Bulletin | Year: 2011

To determine whether the antihypertensive effect of nattokinase is associated with the protease activity of this enzyme, we compared nattokinase with the fragments derived from nattokinase, which possessed no protease activity, in terms of the effect on hypertension in spontaneously hypertensive rats (SHR). In the continuous oral administration test, the groups were given a basic diet alone (control), the basic diet containing nattokinase (0.2, 2.6 mg/g diet) or the basic diet containing the fragments derived from nattokinase (0.2, 0.6 mg/g diet). The group fed the basic diet containing high-dosage nattokinase (2.6 mg/g diet) showed significant reductions in systolic blood pressure (SBP), diastolic blood pressure (DBP) and plasma fibrinogen level, compared with control group and no influence on activities of renin and angiotensin-converting enzyme (ACE, EC 3.4.15.1), and plasma angiotensin II level in the renin-angiotensin system. The treatment of the basic diet containing high-dosage fragments (0.6 mg/g diet) significantly decreased SBP, DBP and plasma angiotensin II level in plasma but the treatment did not influence on plasma fibrinogen level. These results suggest that nattokinase and its fragments are different from each other in the mechanism to reduce hypertension. Nattokinase, retained its protease activity after absorbance across the intestines, may decrease blood pressure through cleavage of fibrinogen in plasma. The fragments, which absorbed as nattokinase-degradation products, prevents the elevation of plasma angiotensin II level to suppress hypertension. © 2011 Pharmaceutical Society of Japan.


Murakami K.,Aichi Medical University | Yamanaka N.,Japan Tounou Clinic | Ohnishi K.,Japan Bio Science Laboratory Co. | Fukayama M.,Aichi Medical University | Yoshino M.,Aichi Medical University
Food and Function | Year: 2012

Angiotensin I converting enzyme (ACE) was inhibited by the culture medium of Bacillus subtilis subsp. natto, which ferments boiled soy beans to natto, a Japanese traditional food. Subtilisin NAT (nattokinase) produced by B. subtilis also inhibited ACE, and the inhibition was markedly stimulated by heat treatment of subtilisin at 120 °C for 15min. Inhibition of ACE by subtilisin was of a mixed type: the decrease in Vmax and the increase in Km value. SDS-polyacrylamide gel electrophoresis showed that heat treatment of subtilisin caused inactivation with fragmentation of the enzyme protein into small peptides. The inhibitory action of subtilisin was not due to an enzymatic action of protease, but may be ascribed to the potent ACE-inhibitory peptides such as LY and FY, amino acid sequences in subtilisin. HPLC-MS analysis of heat-inactivated subtilisin confirmed that LY and FY were liberated by fragmentation of the enzyme. Inhibition of ACE by subtilisin and its degradation peptides such as LY and FY may participate in the suppression of blood pressure by ingestion of natto. © The Royal Society of Chemistry 2012.


Trademark
Japan Bio Science Laboratory Co. | Date: 2014-11-07

Plant extracts for weight management, namely, powdered or liquid extracts of Angelica keiskei, used in the manufacture of food supplements and processed foods. Plant extracts for weight management, namely, powdered or liquid extracts of Angelica keiskei, sold as integral components of food supplements. Plant extracts for weight management, namely, powdered or liquid extracts of Angelica keiskei, sold as separate ingredients for use in food supplements and processed foods; plant extracts for weight management, namely, powdered or liquid extracts of Angelica keiskei sold as an integral component of processed foods in the nature of food packaged combinations consisting primarily of cheese, meat and/or processed fruit.


Trademark
Japan BioScience Laboratory Co. | Date: 2013-03-29

Food supplements, namely, food supplements containing nattokinase, bacillus natto or natto used to improve cardiovascular health; drug delivery agents in the form of capsules and gels containing nattokinase, bacillus natto or natto used to improve cardiovascular health; fortified edible wafer paper containing nattokinase, bacillus natto or natto used to improve cardiovascular health; fortified powdered milk for infants containing nattokinase, bacillus natto or natto used to improve cardiovascular health; fortified beverages for infants containing nattokinase, bacillus natto or natto used to improve cardiovascular health; and foods for infants, namely, bars or wafers containing nattokinase, bacillus natto or natto used to improve cardiovascular health.

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