Jangwon Co.

Jeju, South Korea

Jangwon Co.

Jeju, South Korea
Time filter
Source Type

Chang Y.,Jeju National University | Oh E.U.,Jeju National University | Lee M.S.,Jangwon Co. | Kim H.B.,Biomedic Co. | And 2 more authors.
Euphytica | Year: 2017

Based on double pseudo-testcross theory, a population of 79 F1 clones, which was derived from a crossing of two tea cultivars using a Japanese tea cultivar “Fushun” (Camellia sinensis) as female parent and a Korean tea cultivar “Kemsull” (C. sinensis) as male parent, was applied to construct a genetic linkage map with different molecular markers. Previously, 143 random amplified polymorphism DNA (RAPD) markers and 11 simple sequence repeat (SSR) loci developed from 41 decamer random primers and 60 published SSR primer pairs, respectively, were used into our mapping analysis. In the study of amplified fragment length polymorphism (AFLP), 2439 bands were generated from 27 primer combinations with an average number of 90.3 bands for each, of which 495 (20.3%) were polymorphic. The majority of those polymorphic markers segregated in accordance with Mendelian segregation ratios at p < 0.01 for 400 (80.8%) or at p < 0.05 for 382 (77.2%), of which 136 (34.0%) or 131 (34.3%) were at 3:1 segregation ratios and 264 (66.0%) or 251 (65.7%) were at 1:1 segregation ratios at p < 0.01 and p < 0.05, respectively. For developing more SSR markers, the transcriptome sequences of flowers and leaves of two parents were obtained using high throughput RNA sequencing and compared. Afterwards, 1800 potential polymorphic SSR markers were successfully developed and 296 of them were selected and experimentally validated with a subset of tea plants (including two parents and six F1 offspring), from which 75 (25.3%) were repeatably amplified and polymorphic between two parents. From that, 29 (38.7%) newly mined SSR markers were heterozygous in “Fushun” and/or “Kemsull” and showed segregant genotypes in F1 seedlings and were adoptable by JoinMap 4.0. Totally, 678 markers including 143 RAPDs, 11 public SSRs, 495 AFLPs, and 29 newly mined SSRs were conjointly used to construct a combined linkage map for tea plant. The new genetic map located 79 RAPDs, 5 public SSRs, 214 AFLPs, and 11 new SSRs developed from RNA seq technique and covered 1441.6 cM with an average distance of 4.7 cM between two adjacent markers. This map will lay a foundation for qualitative or quantitative trait loci (QTLs) analysis of important agronomic traits for tea plant in the future study. © 2017, Springer Science+Business Media B.V.

Song K.-J.,Jeju National University | Beak D.-C.,Jeju National University | Kim Y.-W.,Jeju National University | Kim Y.-G.,Jangwon Co. | And 3 more authors.
Journal of Food Science and Nutrition | Year: 2010

Caffeine and catechins from the Yabukita, Yutakamidori, Saemidori, Okumidori, and Fushun varieties of tea leaves picked during different harvesting seasons from April to October were evaluated using HPLC. Total content of catechins increased greatly with the later harvesting time of tea leaves (i.e., picking the leaves in September versus in April) and decreased slightly after September. Yabukita tea leaves picked in August contained 43.1 mg% catechins including EGC, EC, ECG, and EGCG, with the ECGC levels constituting greater than 50% of those four compounds. Yutakamidori and Okumidori varieties picked in September contained the highest catechin values, at 43.6 mg% and 31.0 mg%, respectively. Fushun and Saemidori varieties contained lower catechin concentrations of 14.5 mg% (July) and 11.7 mg% (August) compared to other varieties. The EGCG levels gradually decreased in the late harvesting season, while levels of the other catechins, EC, EGC, and ECG, gradually increased. All varieties of green tea showed a gradual decrease in caffeine content toward the end of our harvesting efforts in October, with levels of 58̃68 mg% in April and 28̃57 mg% in October. Yabukita, Saemidori, and Okumidori varieties reached their highest caffeine levels in late spring/early summer, with Yabukita and Okumidori varieties reaching a high of 73.4% and 63.5% caffeine, respectively, in May, and Saemidori at 64.0% in June. In particular, Fushun still contained high caffeine of 66.8 mg% (September) during the late harvesting season. Copyright © 2007 KFN.

Lee J.-E.,Korea University | Lee B.-J.,Amore Pacific | Hwang J.-A.,Amore Pacific | Ko K.-S.,Jangwon Co. | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2011

The dependence of global green tea metabolome on plucking positions was investigated through 1H nuclear magnetic resonance (NMR) analysis coupled with multivariate statistical data set. Pattern recognition methods, such as principal component analysis (PCA) and orthogonal projection on latent structure-discriminant analysis (OPLS-DA), were employed for a finding metabolic discrimination among fresh green tea leaves plucked at different positions from young to old leaves. In addition to clear metabolic discrimination among green tea leaves, elevation in theanine, caffeine, and gallic acid levels but reductions in catechins, such as epicatechin (EC), epigallocatechin (EGC), epicatechin-3-gallate (ECG), and epigallocatechin-3-gallate (EGCG), glucose, and sucrose levels were observed, as the green tea plant grows up. On the other hand, the younger the green tea leaf is, the more theanine, caffeine, and gallic acid but the lesser catechins accumlated in the green tea leaf, revealing a reverse assocation between theanine and catechins levels due to incorporaton of theanine into catechins with growing up green tea plant. Moreover, as compared to the tea leaf, the observation of marked high levels of theanine and low levels of catechins in green tea stems exhibited a distinct tea plant metabolism between the tea leaf and the stem. This metabolomic approach highlights taking insight to global metabolic dependence of green tea leaf on plucking position, thereby providing distinct information on green tea production with specific tea quality. © 2011 American Chemical Society.

Oh J.,Sungkyunkwan University | Kim J.H.,Sungkyunkwan University | Park J.G.,Sungkyunkwan University | Yi Y.-S.,Sungkyunkwan University | And 8 more authors.
Mediators of Inflammation | Year: 2013

Lutein is a naturally occurring carotenoid with antioxidative, antitumorigenic, antiangiogenic, photoprotective, hepatoprotective, and neuroprotective properties. Although the anti-inflammatory effects of lutein have previously been described, the mechanism of its anti-inflammatory action has not been fully elucidated. Therefore, in the present study, we aimed to investigate the regulatory activity of lutein in the inflammatory responses of skin-derived keratinocytes or macrophages and to elucidate the mechanism of its inhibitory action. Lutein significantly reduced several skin inflammatory responses, including increased expression of interleukin-(IL-) 6 from LPS-treated macrophages, upregulation of cyclooxygenase-(COX-) 2 from interferon-γ/tumor necrosis-factor-(TNF-) α-treated HaCaT cells, and the enhancement of matrix-metallopeptidase-(MMP-) 9 level in UV-irradiated keratinocytes. By evaluating the intracellular signaling pathway and the nuclear transcription factor levels, we determined that lutein inhibited the activation of redox-sensitive AP-1 pathway by suppressing the activation of p38 and c-Jun-N-terminal kinase (JNK). Evaluation of the radical and ROS scavenging activities further revealed that lutein was able to act as a strong anti-oxidant. Taken together, our findings strongly suggest that lutein-mediated AP-1 suppression and anti-inflammatory activity are the result of its strong antioxidative and p38/JNK inhibitory activities. These findings can be applied for the preparation of anti-inflammatory and cosmetic remedies for inflammatory diseases of the skin. © 2013 Jueun Oh et al.

Kim W.W.,Kangwon National University | Rho H.S.,Amore Pacific | Hong Y.D.,Amore Pacific | Yeom M.H.,Amore Pacific | And 5 more authors.
Molecules | Year: 2013

Seed oil triacylglycerol (TAG) composition of 32 soybean varieties were determined and compared using 1H-NMR. The contents of linolenic (Ln), linoleic (L), and oleic (O) ranged from 10.7% to 19.3%, 37.4%-50.1%, and 15.7%-34.1%, respectively. As is evident, linoleic acid was the major fatty acid of soybean oil. Compositional differences among the varieties were observed. Natural oils containing unsaturated groups have been regarded as important nutrient and cosmetic ingredients because of their various biological activities. The TAG profiles of the soy bean oils could be useful for distinguishing the origin of seeds and controlling the quality of soybean oils. To the best of our knowledge, this is the first study in which the TAG composition of various soybean oils has been analyzed using the 1H-NMR method. © 2013 by the authors.

Kim H.-J.,Amore Pacific | Kim H.-J.,Sungkyunkwan University | Chang H.,Amore Pacific | Han S.H.,Sungkyunkwan University | And 10 more authors.
International Journal of Molecular Sciences | Year: 2014

(-)-Epigallocatechin-3-O-gallate (EGCG) has long been known as a potent inducer of keratinocyte differentiation. Although its molecular mechanisms have been extensively studied, its actions on human skin remain to be elucidated. In this study, we demonstrated that methylated EGCG and EGCG increase the expression of klotho, and that klotho functions as a downstream target of EGCG and methylated EGCG in keratinocyte differentiation. We demonstrated that methylated EGCG3 and EGCG induce morphological changes in normal human epidermal keratinocytes (NHEKs) that are related to up-regulation of klotho expression. We also demonstrated that a klotho-induced keratinocyte differentiation marker in NHEKs is inhibited by H-89, a protein kinase (PKA) inhibitor. These results suggest that methylated EGCG and EGCG may function as inducers of keratinocyte differentiation via transcriptional regulation of the klotho protein. © 2014 by the authors; licensee MDPI, Basel, Switzerland.

Lee M.-S.,Jangwon Co. | Hwang Y.-S.,Kangwon National University | Lee J.,U.S. Department of Agriculture | Choung M.-G.,Kangwon National University | Choung M.-G.,University of Texas at Arlington
Food Chemistry | Year: 2014

Near-infrared reflectance spectroscopy (NIRS) was used to determine the contents of caffeine and nine individual catechins in tea leaves. A total of 665 samples were scanned by NIRS, and also by high performance liquid chromatography coupled to a diode array detector to determine the contents of caffeine and nine individual catechins. The calibration models for caffeine, EGC, C, EGCG, EC, ECG, and total catechins had high r2 (more than 0.90) and RSP (the ratio of standard deviation of reference data to SEP(C) in the external validation set) values (more than 4.1), indicating a good correlation between reference values and NIRS predicted values. In contrast, the calibration models of GC and EGCG-3Me had low r2 and RSP values (below 0.8 and 2.0). Therefore, these results suggest that NIRS could be applied for the rapid determination of the contents of caffeine, EGC, C, EGCG, EC, ECG, and total catechins in tea leaves for breeding programs that develop high-quality tea plants. © 2014 Elsevier Ltd. All rights reserved.

Choung M.-G.,Kangwon National University | Hwang Y.-S.,Kangwon National University | Lee M.-S.,Jangwon Co. | Lee J.,U.S. Department of Agriculture | And 2 more authors.
International Journal of Food Science and Technology | Year: 2014

Catechins from green tea (Camellia sinensis L.) have received considerable attention due to their beneficial effects on human health, such as antioxidant and anticancer activities. Optimisation of extraction conditions of the catechins from green tea leaves was performed using different solvents (ethanol or distilled water), different extraction methods (ultrasound-assisted, room temperature or reflux extractions) and various extraction times (0.5-24 h). The optimal extraction conditions were determined using 40% ethanol with ultrasound-assisted extraction method for 2 h at 40 °C. In addition, two isolation methods for the recovery of catechins from green tea extracts were compared using different solvent combinations (chloroform/ethyl acetate versus ethyl acetate/dichloromethane). The results showed that the ethyl acetate/dichloromethane system could achieve much higher content of catechins than the other isolation approaches, indicating the method that extract catechins first with organic solvent such as ethyl acetate before removing caffeine is much effective than removing caffeine first when organic solvents are used for the recovery of catechins without caffeine from green tea extracts. © 2013 Institute of Food Science and Technology.

Choung M.-G.,Kangwon National University | Lee M.-S.,Jangwon Co.
Food Science and Biotechnology | Year: 2011

The amount of catechins and caffeine in green tea are considered a standard of quality evaluation for green tea. The objectives of this study were to investigate the optimal conditions for simultaneous extraction of catechins and caffeine and to compare their quantity and composition within the exotic tea varieties. Moreover, 8 tea varieties of exotic origin were tested with the optimal extraction conditions found in this study. Regarding the caffeine and catechins contents, a 2 h room temperature extraction using a 2% phosphoric acid-40% EtOH solution was the most suitable simultaneous extraction method. The total catechins and caffeine contents of the tea leaves ranged 44.25-64.80 and 9.26-13.11 mg/g, respectively. The optimal extraction method of catechins and caffeine in tea leaves may be used for further studies and breeding of high quality tea plants. © KoSFoST and Springer 2011.

Loading Jangwon Co. collaborators
Loading Jangwon Co. collaborators