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Latlief G.A.,James ley Veterans Medical Center | Highsmith M.J.,University of South Florida
Topics in Geriatric Rehabilitation | Year: 2014

Lower extremity amputation is known to compromise gait, balance, and overall capacity to react to slips and trips. Given an estimated 1.8 million persons in the United States living with limb loss with an expectation for this number to double by the year 2050, the need for rehabilitative care providers to evaluate and develop treatment plans to minimize the potential for falls will surely rise. Objective measurement of fall risk and evaluation of the effect of targeted treatment interventions are essential to enhancing the quality of rehabilitative care. As a means to this end, the development and use of objective outcome measures that are relevant to the amputee population are necessary. The purpose of this article is to identify and describe these tools and critically review their respective clinical strengths and limitations. © 2014 Lippincott Williams and Wilkins.

Yu H.,Harvard University | Fujii N.L.,Harvard University | Toyoda T.,Harvard University | An D.,Harvard University | And 5 more authors.
Physiological Reports | Year: 2015

Exercise increases skeletal muscle glucose uptake, but the underlying mechanisms are only partially understood. The atypical protein kinase C (PKC) isoforms λ and ζ (PKC-λ/ζ) have been shown to be necessary for insulin-, AICAR-, and metformin-stimulated glucose uptake in skeletal muscle, but not for treadmill exercise-stimulated muscle glucose uptake. To investigate if PKC-λ/ζ activity is required for contraction-stimulated muscle glucose uptake, we used mice with tibialis anterior muscle-specific overexpression of an empty vector (WT), wild-type PKC-ζ (PKC-ζWT), or an enzymatically inactive T410A-PKC-ζ mutant (PKC-ζT410A). We also studied skeletal muscle-specific PKC-λ knockout (MλKO) mice. Basal glucose uptake was similar between WT, PKC-ζWT, and PKC-ζT410A tibialis anterior muscles. In contrast, in situ contraction-stimulated glucose uptake was increased in PKC-ζT410A tibialis anterior muscles compared to WT or PKC-ζWT tibialis anterior muscles. Furthermore, in vitro contraction-stimulated glucose uptake was greater in soleus muscles of MλKO mice than WT controls. Thus, loss of PKC-λ/ζ activity increases contraction-stimulated muscle glucose uptake. These data clearly demonstrate that PKC-λζ activity is not necessary for contraction-stimulated glucose uptake. © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

Addisu A.,University of South Florida | Addisu A.,Cardiac Hormone Center | Gower Jr. W.R.,University of South Florida | Gower Jr. W.R.,Cardiac Hormone Center | And 5 more authors.
Experimental Biology and Medicine | Year: 2011

Our recent study showed that intravenously administered B-type natriuretic peptide (BNP) decreases gastric emptying and intestinal absorption in mice. We aimed to test whether acute myocardial injury and heart failure have similar effects. Wildtype (WT) and natriuretic peptide receptor type A (NPR-A) knockout (KO) mice underwent cryoinfarction (myocardial infarction [MI]) of the left ventricle (LV) versus sham. LV dysfunction was confirmed by echocardiography. Percent gastric emptying and intestinal absorption were measured and analyzed one and two weeks after infarction, by gavage feeding the mice with fluorescein-isothiocyanate-dextran. Ejection fraction was 48±3% versus 64±2% (P<0.05) and fractional shortening was 24±2% versus 35±2% (P<0.01), MI versus sham, respectively. BNP levels (pg/mL) were 4292±276 one week after MI versus 105±11 in sham (n = 5, P<0.05) and 1964±755 two weeks after MI (n = 5, P<0.05). Gastric emptying was significantly decreased, 68±6% in MI versus 89±3% in sham (n = 5, P<0.05) one week after MI and 82±0.5% versus 98±0.4%, MI versus sham (n = 5, P<0.05), two weeks post-MI. Absorption, measured in relative plasma fluorescence units in WT mice, was 350±79 in MI versus 632±121 in sham (n = 6, P<0.05). KO mice did not show a significant difference in emptying or absorption compared with sham. These findings suggest that MI and LV dysfunction decrease gastric emptying and absorption in mice through a mechanism that involves NPR-A. © 2011 by the Society for Experimental Biology and Medicine.

Maroun E.N.,University of South Florida | Chakrabarti A.,University of South Florida | Baker M.,University of South Florida | Canchi D.,University of South Florida | And 3 more authors.
Infectious Diseases in Clinical Practice | Year: 2012

INTRODUCTION: Pyogenic liver abscess accounts for 80% of hepatic abscess cases in the United States. Increasing rates of anaerobic involvement has been reported since 1974. Fusobacterium species are among the most commonly encountered anaerobes, although it is a rare finding to see Fusobacterium species as a cause of liver abscess. CASE PRESENTATION: A 60-year-old man with a history of 100 pack year of tobacco use and chronic obstructive pulmonary disease, presented with radiological findings for metastatic liver malignancy. This was an incidental finding when the patient had a computed tomographic pulmonary angiogram to rule out pulmonary embolism. The incidental findings were followed up with radiological testing, a colonoscopy, endoscopy, and 2 biopsies. The patient's condition was finally diagnosed as a liver abscess due to Fusobacterium and was successfully treated with a prolonged course of metronidazole. CONCLUSION: Pyogenic liver abscess can mimic metastatic malignancy and should be considered when multiple hepatic lesions are seen on an abdominal computed tomography or other compatible diagnostic imaging. Copyright © 2012 by Lippincott Williams & Wilkins.

Ostendorf B.N.,University Hospital Freiburg | Oehler R.L.,University of South Florida | Oehler R.L.,James ley Veterans Medical Center | Greene J.N.,University of South Florida
Infectious Diseases in Clinical Practice | Year: 2011

Staphylococcus intermedius is known primarily for its role as a commensal and pathogen in dogs and a variety of other animals. It has also been reported as a cause of human infection in a limited number of cases. Here, we report a case of S. intermedius wound infection in a 47-year-old woman with postradiation skin changes. Transmission occurred most likely through direct skin contact with her pet dog. This case highlights the possibility of pet-transmitted infections in predisposed patients and should raise awareness of possibly drug-resistant pathogens in animal reservoirs. © 2011 by Lippincott Williams & Wilkins.

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