Bhupinder B.,Lovely Professional University |
Sarita J.,Jaipur National University
International Journal of Drug Development and Research | Year: 2012
Rizatriptan Benzoate, a serotonin 5-HT1 receptor agonist is a new generation antimigraine drug which has oral bioavailability of 47% due to hepatic first pass metabolism. The present study investigated the possibility of developing Rizatriptan benzoate fast dissolving sublingual films allowing fast, reproducible drug dissolution in the oral cavity, thus bypassing first pass metabolism to provide rapid onset of action of the drug. The fast dissolving films were prepared by solvent casting method. Low viscosity grade of hydroxylpropyl methylcellulose (HPMC E 15) and maltodextrin were used in combination as film forming polymer, due to their hydrophilic nature and palatable taste. To decrease the disintegration time of formulations sodium starch glycolate was used as disintegrating agent. Glycerol, mannitol, aspartame and sodium lauryl sulphate were used as a cooling agent, sweetening agent and oral penetration enhancer respectively. All the films formulations (F1-F8) was evaluated for their thickness, weight variations, tensile strength, percentage elongation, folding endurance, surface pH, in-vitro disintegration, drug content, in-vitro drug release and ex-vivo permeation. Disintegration time showed by the formulations was found to be in range of 25-50 sec. Formulations F1 and F2 showed 90% in-vitro drug release within 7 min and 61% ex-vivo drug permeation within16 min. The film showed an excellent stability at least for 4 weeks when stored at 40° C and 75% in humidity. © 2010 IJDDR.
Vemula V.R.B.,Pacific University at Udaipur |
Sharma P.K.,Jaipur National University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013
Objective: To develop a new validated reverse phase high performance liquid chromatography method for the simultaneous estimation of Imipramine and Diazepam in tablet formulation. Methods: The chromatographic analysis was carried out on X Bridge C18 column (150×4.6 mm, 5μ), phosphate buffer with pH 3.4 & Acetonitrile (55:45) as mobile phase, at a flow rate of 1 ml/min and detected at wave length 250 nm. Results: The retention times for Imipramine and Diazepam were 2.2 min and 5.6 min respectively. The percentage recoveries for Imipramine and Diazepam were 99.02-100.87% and 99.36-101.18% respectively. The regression value for both the drugs was found to be 0.999, which showed the linear response from 62.5-625μg/ml for Imipramine and 12.5-125μg/ml for Diazepam. Conclusion: This method was fast, accurate, precise and sensitive hence it can be employed for routine analysis of tablets containing both drugs.
Singhal M.,Jaipur National University |
Ratra P.,Jaipur National University
Global Journal of Pharmacology | Year: 2013
Musa acuminate peel (Plantain peel) extract was evaluated for their antioxidant potential using the DPPH scavenging method, Total phenolic content determination and total flavonoid determination. Effect of extract on haematological parameters like total leukocyte count and total lymphocyte count of Wistar albino rats was also investigated. The result showed that methanolic extract is a more potent antioxidant than the hexane extract. Both extracts showed DPPH scavenging effect in dose dependent manner. Total flavonoid and total phenolic are higher in methanolic extract as compared to hexane extract of banana. Both the extracts significantly increased the total leukocyte and the percentage of lymphocyte in dose dependent manner. © IDOSI Publications, 2013.
Gupta H.,Jamia Hamdard University |
Sharma A.,Jaipur National University
International Journal of Drug Delivery | Year: 2011
Vaginal preparations, although generally perceived as safer most, still they are associated with a number of problems, including multiple days of dosing, dripping, leakage and messiness, causing discomfort to users and expulsion due to the self-cleansing action of the vaginal tract. These limitations lead to poor patient compliance and failure of the desired therapeutic effects. For effective vaginal delivery of antimicrobial agents, the drug delivery system should reside at the site of infection for a prolonged period of time. In our present work, we have developed and optimized a chitosan (bioadhesive and permeation enhancer) and gellan gum (ion activated gelling polymer) based in situ gel system of clindamycin for vaginal application. The developed formulation was characterized for various in-vitro parameters e.g. clarity, refractive index, pH, isotonicity, sterility, viscosity, drug release profile, statistical release kinetics, bioadhesive force, retention time, microbial efficacy, irritation test and stability studies. To simulate vaginal conditions, a synthetic membrane (cellophane hydrated with modified simulated vaginal fluid) and sheep vaginal mucosa were used as model membranes. The developed formulation was found to be non irritant, bioadhesive with good retention properties. Developed formulation shows matrix model release kinetic by PCP disso software. The developed formulation is thus a viable alternative to conventional vaginal dosage forms. © arjournals.org.
Sharma A.K.,Jaipur National University |
Deshwal N.,Jaipur National University
International Journal of PharmTech Research | Year: 2011
Butea monosperma popularly known as Flame of the Forest, Dhak, Palash or 'Bastard teak' which has immense potential and appears to have a broad spectrum of activity on several ailments. Previous phytochemical examination of this plant indicated the presence of various constituents some of these are Cajanin and isoformononetin; Stigmasterol; Butin; two known flavonoids, isobutrin (3, 4, 2′, 4′-tetrahydroxychalcone-3, 4′-diglucoside), and the less active butrin (7, 3′, 4′-trihydroxyflavanone-7, 3′-diglucoside); free sugars and free amino acids and (-)-3-hydroxy-9-methoxypterocarpan[(-)-medicarpin which were isolated from stem-bark extract; bark; seeds; flower; the petroleum ether extract of flowers and petroleum and ethyl acetate extract of stem bark. Its reported pharmacological properties include anthelmintic, anticonceptive, anticonvulsive, antidiabetic, antidiarrhoeal, antiestrogenic and antifertility, anti-inflammatory, antimicrobial, antifungal, antibacterial, antistress, chemopreventive, haemaggultinating, hepatoprotective, radical scavenging, Thyroid inhibitory, antiperoxidative and hypoglycemic effects and wound healing activities. Other then these an Ayurvedic herbal medicine which was prepared from Piper longum (Pippali) and Butea monosperma (Palash) has significant activity against Giardiasis.
Raina A.P.,National Bureau of Plant Genetic Resources |
Khatri R.,Jaipur National University
Indian Journal of Pharmaceutical Sciences | Year: 2011
Mucuna pruriens Linn. is an important medicinal plant used for treatment of Parkinson's disease and many others in ancient Indian medical system. L-DOPA extracted from seeds of Mucuna is a constituent of more than 200 indigenous drug formulations and is more effective as drug than the synthetic counterpart. A densitometric high performance thin-layer chromatographic (HPTLC) method was developed for quantification of L-DOPA content present in the seeds extract. The method involves separation of L-DOPA on precoated silica gel 60 GF 254 HPTLC plates using a solvent system of n-butanol-acetic-acid- water (4:1:1, v/v) as the mobile phase. Quantification was done at 280 nm using absorbance reflectance mode. Linearity was found in the concentration range of 100 to 1000 ng/spot with the correlation coefficient value of 0.9980. The method was validated for accuracy, precision and repeatability. Mean recovery was 100.89%. The LOD and LOQ for L-DOPA determination were found to be 3.41 ng/spot and 10.35 ng/spot respectively. The proposed HPTLC method was found to be precise, specific and accurate for quantitative determination of L-DOPA. It can be used for rapid screening of large germplasm collections of Mucuna pruriens for L-DOPA content. The method was used to study variation in fifteen accessions of Mucuna germplasm collected from different geographical regions.
Tiwari K.,Jaipur National University
Pakistan Journal of Biological Sciences | Year: 2013
The present study discusses the frequency distribution and genetic diversity of novel fungal endopyte Alternaria alternata within the Pongammia pinnata plant samples. A total of ten plant samples of Pongammia pinnata, Pierre. (Karanja) were collected from specific locations of Sanganer region of Rajasthan for the isolation of fungal endophytes. Of these, maximum frequency of Alternaria alternata (22.29%) were recorded which are morphologically similar but ecologically variant. Efficacy of randomly amplified polymorphic DNA (RAPD), were assessed in seventeen individuals of the primers was GCC 180 where as 10 bands were generated by GCC 181. The similarity coefficient matrix generated for the primers was subjected to algorithm UPGMA (Unweighted Pair Group Method Analysis) and clusters were generated using NTSYS 2.02 pc program. To stabilize the level of relatedness among the seventeen ecologically variant Alternaria alternata accessions, the dendrogram was constructed, which showed that all the isolates were diversified endophytically with in the plant Pongamia pinnata. © 2013 Asian Network for Scientific Information.
Sharma K.K.,Malaviya National Institute of Technology, Jaipur |
Sharma S.,Jaipur National University
Journal of Optics | Year: 2012
The problem of signal reconstruction using the samples of a signal f(t) and/or signals related to f(t) has been widely investigated in the literature. However, none of the existing sampling expansions deals with the signal reconstruction in the linear canonical transform (LCT) domain using undersampled samples taken in several LCT domains. In this paper, we present some novel sampling expansions involving signal reconstruction using samples of the linear canonical transform (LCT) of the signal in some specific LCT domains. © 2012 IOP Publishing Ltd.
Bhati-Kushwaha H.,Jaipur National University |
Malik C.P.,Jaipur National University
Indian Journal of Experimental Biology | Year: 2014
A simple and eco-friendly method for the synthesis of biogenic nanoparticles (NP's) using an aqueous solution of T. procumbens fresh plant extract (leaf and stem) as a bioreductant is reported. The prepared biogenic nanoparticles were well characterized using U.V. visible spectroscopy, scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy. The particles were confirmed to be elemental crystal by X-ray diffraction. The potential applications of biosynthesized nanoparticles as antimicrobial (antibacterial and antifungal) against pathogens Escherichia coli, Vibrio cholerae, Aspergillus niger and Aspergillus flavus were demonstrated.
Bairwa R.,Jaipur National University |
Sodha R.S.,Jaipur National University |
Rajawat B.S.,Jaipur National University
Pharmacognosy Reviews | Year: 2012
Trachyspermum ammi commonly known as 'Ajwain' is distributed throughout India and is mostly cultivated in Gujarat and Rajasthan. The fruit possesses stimulant, antispasmodic and carminative properties and is used traditionally as an important remedial agent for flatulence, atonic dyspepsia, diarrhea, abdominal tumors, abdominal pains, piles, and bronchial problems, lack of appetite, galactogogue, asthma and amenorrhoea. Medicinally, it has been proven to possess various pharmacological activities like antifungal, antioxidant, antimicrobial, antinociceptive, cytotoxic, hypolipidemic, antihypertensive, antispasmodic, broncho-dilating actions, antilithiasis, diuretic, abortifacient, antitussive, nematicidal, anthelmintic and antifilarial. Further, studies reveal the presence of various phytochemical constituents mainly carbohydrates, glycosides, saponins, phenolic compounds, volatile oil (thymol, -terpinene, para-cymene, and - and -pinene), protein, fat, fiber and mineral matter containing calcium, phosphorous, iron and nicotinic acid. These studies reveal that T. ammi is a source of medicinally active compounds and have various pharmacological effects; hence, it is encouraging to nd its new therapeutic uses.