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Desai J.,Narsee Monjee Institute of Management and Higher Studies | Belemkar S.,Narsee Monjee Institute of Management and Higher Studies | Patel M.,Jai Research Foundation
International Journal of Phytomedicine | Year: 2015

Context: Trigonella foenum graecum L. (Fabaceae) seeds have been extensively used in Ayurveda and Unani medicine. It has been used as a traditional medicine for a household remedy against various human ailments. The seeds have been shown to possess hypoglycemic, hypocholesterolaemic and antioxidant activity. Objective: The present study is to evaluate whether ovariectomised (OVX) Wistar rats could form an experimental model of postmenopausal hyperlipidemia and to evaluate the antihyperlipidemic potential of Atorvastatin (AT) and Ethyl acetate extract of Trigonella foenum graecum L. Seeds (ET) in OVX Wistar rats and toxicity associated with it. Methods: Ovariectomy was performed to mimic the postmenopausal hyperlipidemic condition in Wistar rats. The effects of AT and ET on body weight, weight of uterus and the levels of total cholesterol (TC), triglycerides (TG), high density lipoproteins (HDL), low density lipoproteins (LDL), very low density lipoprotein (VLDL) were also evaluated. Results: The rats in OVX control group showed 51% weight gain when compared with vehicle control group. The serum TC, TG, LDL, VLDL of OVX control group was found to be increased significantly; serum HDL level was reduced and atrophy of uterus was observed in comparison with vehicle control group. The ET showed the significant (P<0.05, P<0.01) antihyperlipidemic potency when compared with AT & proportional antihyperlipidemic potency in comparison with Estradiol benzoate (EB). Conclusion: These findings confirm the bilateral OVX Wistar rats as a model of postmenopausal hypercholesterolemia. The significant antihyperlipidemic activity of ET thus lends pharmacological credence to the suggested use of the plant as a safe natural remedy in the treatment of postmenopausal hyperlipidemia. © 2015, Advanced Research Journals. All rights reserved. Source


Samnani P.,M. S. University of Baroda | Vishwakarma K.,Jai Research Foundation
Research Journal of Chemistry and Environment | Year: 2013

This study was conducted to determine the hydrolysis rate and half-life of imidacloprid technical in buffer solution of pH 4.0, 7.0 and 9.0 at 50 ± 0.5 °C. In the preliminary test, the initial concentration of Imidacloprid (0 hour) in buffer solutions at pH 4.0, 7.0 and 9.0 was 3.93, 3.89 and 3.99 μg/mL respectively. The imidacloprid concentration after 2 hours of incubation at 50 ± 0.5 °C in buffer solutions of pH 4.0, 7.0 and 9.0, was 3.94, 3.86 and 3.95 μg/mL respectively. The per cent hydrolysis data revealed that degradation of imidacloprid at pH 4.0, 7.0 and 9.0 was less than 10% in the preliminary test (5 days). Hence, it is concluded that the theoretical half-life of imidacloprid TC is >1 year at 25 °C and imidacloprid is hydrolytically stable and unlikely to hydrolyse under the environmental conditions. Source


Singh S.K.,Jai Research Foundation | Padmaja P.,M. S. University of Baroda | Pandey S.Y.,Jai Research Foundation
Journal of Separation Science | Year: 2014

A fast and an efficient ultrasound-assisted extraction technique using a lower density extraction solvent than water was developed for the trace-level determination of tebuconazole in garlic, soil and water samples followed by capillary gas chromatography combined with nitrogen-phosphorous selective detector (GC-NPD). In this approach, ultrasound radiation was applied to accelerate the emulsification of the ethyl acetate in aqueous samples to enhance the extraction efficiency of tebuconazole without requiring extra partitioning or cleaning, and the use of capillary GC-NPD was a more sensitive detection technique for organonitrogen pesticides. The experimental results indicate an excellent linear relationship between peak area and concentration obtained in the range 1-50 μg/kg or μg/L. The limit of detection (S/N, 3 ± 0.5) and limit of quantification (S/N, 7.5 ± 2.5) were obtained in the range 0.2-3 and 1-10 μg/kg or μg/L. Good spiked recoveries were achieved from ranges 95.55-101.26%, 96.28-99.33% and 95.04-105.15% in garlic, Nanivaliyal soil and Par River water, respectively, at levels 5 and 20 μg/kg or μg/L, and the method precision (% RSD) was ≤5%. Our results demonstrate that the proposed technique is a viable alternative for the determination of tebuconazole in complex samples. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Verma R.,Jai Research Foundation | Brahmankar M.,Jai Research Foundation | Kushwah L.,Jai Research Foundation | Suresh B.,M. S. University of Baroda
Environmental Toxicology and Pharmacology | Year: 2013

The present study examined the protective effect of sulindac on bleomycin-induced lung fibrosis in rats. Animals were divided into saline group, bleomycin group (single intra-tracheal instillation of bleomycin) and bleomycin. +. sulindac (orally from day 1 to day 20). Bleomycin administration reduced the body weight, altered antioxidant status (such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione) while it increased the lung weight, hydroxyproline content, collagen deposition and lipid peroxidation. However, simultaneous administration of sulindac improved the body weight, antioxidant status and decreased the collagen deposition in lungs. Moreover, the levels of inflammatory cytokine tumour necrosis factor-α increased in bleomycin-induced group, whereas, on treatment with sulindac the levels of tumour necrosis factor-α were found reduced. Finally, histological evidence also supported the ability of sulindac to inhibit bleomycin-induced lung fibrosis. The results of the present study indicate that sulindac can be used as an agent against bleomycin-induced pulmonary fibrosis. © 2013 Elsevier B.V. Source


Samnani P.,M. S. University of Baroda | Vishwakarma K.,Jai Research Foundation | Pandey S.Y.,Jai Research Foundation
Bulletin of Environmental Contamination and Toxicology | Year: 2011

A rapid, sensitive and reliable HPLC method was developed and validated for the determination of imidacloprid residue in water at different pH and in soil. Quantification was performed by reversed phase HPLC system equipped with UV detector. The limit of quantification (LOQ) was found to be 0.02 mg/kg in soil and 0.02 mg/L in water. The limit of detection (LOD) was 0.006 mg/kg in soil and 0.006 mg/L in water. Recoveries for imidacloprid were 95.18%, 94.66%, 95.27% and 94.78% in black, red, sandy loam and clay soils, respectively. Recoveries for imidacloprid from water were 96.86%, 86.14%, and 92.34% at pH values of 4, 7, and 9, respectively. © 2011 Springer Science+Business Media, LLC. Source

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