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Tyrankiewicz U.,Polish Academy of Sciences | Skorka T.,Polish Academy of Sciences | Jablonska M.,Polish Academy of Sciences | Jablonska M.,AGH University of Science and Technology | And 3 more authors.
Journal of Magnetic Resonance Imaging

Purpose: To assess the cardiac response to low (0.15-0.5 mg/kg i.p.) and high (1.5-20 mg/kg i.p.) doses of dobutamine in Tgαq*44 mice with dilated cardiomyopathy at the stage of advanced heart failure. Materials and Methods: Inotropic, lusitropic, and chronotropic response to β1-adrenergic stimulation was assessed by the cine magnetic resonance imaging (MRI) protocol based on the electrocardiogram (ECG)-triggered bright-blood images of one midventricular short-axis slice. Results: In wildtype mice increasing doses of dobutamine resulted in subsequent increase in the left ventricular function and heart rate acceleration, but significant inotropic, lusitropic, and chronotropic cardiac response was observed only after high doses of dobutamine, what is typical. In the Tgαq*44 mice low doses of dobutamine significantly increased inotropic and lusitropic cardiac performance without chronotropic changes. An increased heart rate was observed only after high doses of dobutamine, but then inotropic and lusitropic cardiac functional reserve was lost. Conclusion: We described MRI stress test protocol based on a low and high dose of dobutamine induced response that proves useful in revealing alternation in cardiac function in mice with heart failure. © 2012 Wiley Periodicals, Inc. Source

Galuskin E.V.,University of Silesia | Kusz J.,University of Silesia | Armbruster T.,University of Bern | Galuskina I.O.,University of Silesia | And 3 more authors.
American Mineralogist

Vorlanite (CaU6+)O4 [Fm3m, a = 5.3647(9) Å, V = 154.40(4) Å3, Z = 2] was found in larnite pyrometamorphic rocks of the Hatrurim formation at the Jabel Harmun locality, Judean Desert, Palestinian Autonomy. Vorlanite crystals from these larnite rocks are dark-gray with greenish hue in transmitted light. This color in transmitted light is in contrast to dark-red vorlanite [Fm3m, a = 5.3813(2) Å, V = 155.834(10)Å3, Z = 2] from the type locality Upper Chegem caldera, Northern Caucasus. Heating above 750 °C of dark-gray vorlanite from the Jabel Harmun, as well as dark-red vorlanite from Caucasus, led to formation of yellow trigonal uranate CaUO4. The unusual color of vorlanite from Jabel Harmun is assumed to be related to small impurities of tetravalent uranium. Source

Nemeckova I.,Charles University | Serwadczak A.,Jagiellonian Center for Experimental Therapeutics | Oujo B.,University of Salamanca | Jezkova K.,Charles University | And 8 more authors.

Increased levels of a soluble form of endoglin (sEng) circulating in plasma have been detected in various pathological conditions related to cardiovascular system. High concentration of sEng was also proposed to contribute to the development of endothelial dysfunction, but there is no direct evidence to support this hypothesis. Therefore, in the present work we analyzed whether high sEng levels induce endothelial dysfunction in aorta by using transgenic mice with high expression of human sEng. Transgenic mice with high expression of human sEng on CBAxC57Bl/6J background (Sol-Eng+) and age-matched transgenic littermates that do not develop high levels of human soluble endoglin (control animals in this study) on chow diet were used. As expected, male and female Sol-Eng+ transgenic mice showed higher levels of plasma concentrations of human sEng as well as increased blood arterial pressure, as compared to control animals. Functional analysis either in vivo or ex vivo in isolated aorta demonstrated that the endothelium-dependent vascular function was similar in Sol-Eng+ and control mice. In addition, Western blot analysis showed no differences between Sol-Eng+ and control mice in the protein expression levels of endoglin, endothelial NO-synthase (eNOS) and pro-inflammatory ICAM-1 and VCAM-1 from aorta. Our results demonstrate that high levels of soluble endoglin alone do not induce endothelial dysfunction in Sol-Eng+ mice. However, these data do not rule out the possibility that soluble endoglin might contribute to alteration of endothelial function in combination with other risk factors related to cardiovascular disorders. Copyright: © 2015 Nemeckova et al. Source

Franczyk-Zarow M.,University of Life Sciences in Poznan | Czyzynska I.,University of Life Sciences in Poznan | Drahun A.,University of Life Sciences in Poznan | Maslak E.,Jagiellonian Center for Experimental Therapeutics | And 2 more authors.
European Journal of Lipid Science and Technology

The objective of this study was to evaluate the effect of margarine supplemented with punicic acid (cis-9,trans-11,cis-13 C18:3) (CLnA) compared to CLA and linolenic acid (LnA) in apoE/LDLR-/- mice in two independent experiments: LONG (18 weeks) and SHORT (10 weeks). Liver weight was increased in CLA group in both studies. Hepatic steatosis was found in animals fed CLA and margarine diets but less signs of the steatosis were observed in mice fed LnA and CLnA diets. In LONG, TC was significantly increased in animals fed LnA, whereas LDL+VLDL, HDL and TAG levels were unchanged. In mice fed CLnA the upregulated expression of PPARα and ACO was reported, however, SREBP-1 was 7-fold increased. Punicic acid and CLA isomers were determined both in adipose tissue and liver in CLnA fed mice. No effects of treatments on the area of atherosclerotic plaque were recognized. In conclusion margarine supplemented with CLA significantly increased liver weight and induced steatosis in mice in both studies. Our results did not support the notion that CLnA supplementation to the margarine possesses anti-atheroclerotic effect but it alleviated the liver steatosis and affected the expression of lipid metabolism genes. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Galuskin E.V.,University of Silesia | Galuskina I.O.,University of Silesia | Kusz J.,University of Silesia | Armbruster T.,University of Bern | And 3 more authors.
Mineralogical Magazine

The new mineral species vapnikite, Ca3UO6, was found in larnite pyrometamorphic rocks of the Hatrurim Formation at Jabel Harmun in the Judean desert, Palestinian Autonomy, Israel. Vapnikite is an analogue of the synthetic ordered double-perovskite β-Ca3UO6 and is isostructural with the natural fluorperovskite - cryolite Na3AlF 6. Vapnikite Ca3UO6 (P21/n, Z = 2, a = 5.739(1), b = 5.951(1), c = 8.312(1) Å , β = 90.4(1)°, V = 283.9(1) Å 3) forms yellow-brown xenomorphic grains with a strong vitreous lustre. Small grains up to 20-30 μm in size are wedged between larnite, brownmillerite and ye'elimite. Vapnikite has irregular fracture, cleavage and parting were not observed. The calculated density is 5.322 g cm-3, the microhardness is VHN25 = 534 kg mm -2 (mean of seven measurements) corresponding to the hardness of ∼5 on the Mohs scale. The crystal structure of vapnikite Ca 3UO6 differs from that of its synthetic analogue β-Ca3UO6 by having a larger degree of Ca, U disorder. Vapnikite formed at the high-temperature retrograde stage of pyrometamorphism when larnite rocks were altered by fluids/melts of high alkalinity. © 2014 The Mineralogical Society. Source

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