Jagarlamudi Kuppuswamy Choudary College

andhra Pradesh, India

Jagarlamudi Kuppuswamy Choudary College

andhra Pradesh, India
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Madhavi V.,Tellakula Jalayya Polisetty Somasundaram College | Panchakshari V.,Jagarlamudi Kuppuswamy Choudary College | Prathyusha T.N.,Jagarlamudi Kuppuswamy Choudary College | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
International Journal of Pharmaceutical Sciences Review and Research | Year: 2011

A novel spectrophotometric method for the assay of mesalazine in bulk and dosage form has been developed. The method is based on the diazotization of mesalazine followed by its coupling with resorcinol to form a red colored azo dye. The colored product formed absorbed visible region strongly and optimal detector response was obtained at a wavelength of 490 nm. The analytical parameters and their effects on the reported method are investigated. Beer's law was obeyed in the concentration range of 2-20 μg/ml. The proposed method was directly and easily applied to the tablet dosage forms. Results obtained from the analysis of commercial preparations with the proposed method are in good agreement with those obtained with the reported spectrophotometric method.

Mohiddin S.K.,Acharya Nagarjuna University | Yalavarthi S.B.,Jagarlamudi Kuppuswamy Choudary College | Sharmila S.,Montessori Mahila Kalasala
Advances in Intelligent Systems and Computing | Year: 2017

In order to arm yourself and assess risk correctly in the cloud, carrying out investigations are necessary. Though Cloud Computing is the buzzing technology now a days, with the advent with which cloud computing is developing and providing solutions to the upcoming technologies where on the other side of it there also lies a down fall with respect to theft of data in the cloud, loss of data in the cloud which are related to forensic, these things create an distrustful relation between cloud user and cloud providers, during the past half decade cloud forensic has emerged as a challenging point that has to be solved in cloud computing, the prevailing existing solutions which were suggested at the initial stage of Cloud Forensics were satisfied at that time but those solutions cannot rule now also, due to which they are not up to the mark to give much satisfaction which there by strengthen the faith of users in cloud, and makes Cloud Service Providers to provide the services and make a path to be laid for the coming technologies to utilize the flavors of cloud computing. This paper inculcates the contents related to cloud forensic which are traced out which are being known, to be known. In this paper we have traced out the major causes of Cloud Forensics, and thrown a light on the causes and origins which are helpful for laying a success ladder for Cloud Forensic concepts, and provided relevant content which would be helpful to trace out the solutions which are causing obstacles in the field of Cloud Forensics, utilizing which one can get an overview of the challenges and if considered can definitely lead to a good solution methods which if implemented in a proper way can lead to recovery of lost data as well as to know the real cause of the effect and also in order to reduce their effect. © Springer Nature Singapore Pte Ltd. 2017.

Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College | Tirupatamma P.L.,Jagarlamudi Kuppuswamy Choudary College | Rajitha V.,Jagarlamudi Kuppuswamy Choudary College | Vishnupriya K.,Jagarlamudi Kuppuswamy Choudary College | Jayasree B.,Jagarlamudi Kuppuswamy Choudary College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

Two simple, sensitive, precise and accurate visible spectrophotometric methods have been developed for the assay of ezetimibe in bulk drug and in pharmaceutical formulations. The first method, i.e. the 2, 2'-bipyridyl method, is based on the oxidation of the drug with Fe (III) and the estimation of Fe (II) produced after chelation with 2, 2'-bipyridyl at 530 nm. The second method, i.e. the potassium dichromate method, is based on oxidation of the drug by K2Cr2O7 in acidic medium and the determination of chromium (III) ion produced at 600 nm. Reaction conditions were optimized to achieve the maximum color intensity. The absorbance was found to increase linearly with increase in concentration of ezetimibe, which was corroborated by the calculated regression coefficient value (0.9984-0.9991). Beer's law is obeyed in the concentration range of 2-40 and 10-200 μg/ml for 2, 2'-bipyridyl and K2Cr2O7, respectively. The molar absorptivity, Sandell's sensitivity, limit of detection and limit of quantification have been evaluated. The proposed methods were applied to the quantification of ezetimibe in pharmaceutical formulations. The results obtained by the proposed methods were statistically compared by means of student t-test and by the variance ratio F-test with those of the reported (UV spectrophotometry) method. Common excipients used in pharmaceutical formulations do not interfere in the proposed methods.

Satyanarayana P.V.V.,Acharya Nagarjuna University | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
Pharmaceutical Sciences | Year: 2014

Background: A simple, sensitive, selective and precise stability indicating HPLC method is developed and validated for the assay of ambrisentan in pharmaceutical dosage form. Methods: Separation of the drug was achieved on Agilent Zorbax C18 column (250 mm × 4.6 mm I.D., 5 μm particle size) under the isocratic mode of elution. The solvent system consisted of 0.1M potassium dihydrogen phosphate buffer (pH was adjusted to 4.4 with orthophosphoric acid) and methanol (30:70 v/v) at a flow rate of 1.0 ml/min. The method was carried out in the absorbance mode at 210 nm. The method was statistically validated according to International Conference on Harmonization guideline. Ambrisentan was subjected to stress degradation studies under acidic, basic, oxidative, thermal and photolytic conditions. Results: The system was found to give compact peak for ambrisentan (Retention time is 3.315 min). The method was linear in the range of 1 - 150 μg/ml. The linear regression data for the calibration plot showed good relationship (r2 = 0.9996). The relative standard deviation and mean recovery values at different concentration levels were within limits. The performance of the method was not changed when small variations in the experimental conditions were made. Degradation products resulting from stress degradation studies did not interfere with the detection of ambrisentan. Conclusions: The proposed stability indicting HPLC method is simple, precise, accurate, robust and selective. This method can be used for quantification of ambrisentan in bulk drug and in pharmaceutical dosage forms. Copyright © 2013 by Tabriz University of Medical Sciences.

Kumar N.S.,Acharya Nagarjuna University | Rani A.P.,Acharya Nagarjuna University | Visalakshi T.,Acharya Nagarjuna University | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
Advanced Pharmaceutical Bulletin | Year: 2013

Purpose: Ambrisentan (ABS) is an antihypertensive drug used in the treatment of pulmonary atrial hypertension. The survey of literature for ABS revealed only two spectrophotometric methods for its quantification. The reported methods lack the sensitivity. This study is aimed at developing two sensitive extractive spectrophotometric methods for the determination of ABS in bulk and in tablets. Methods: The proposed methods are based on the formation of colored chloroform extractable ion-pair complexes of ABS with methylene blue (MB method) and safranine O (SO method) in buffered solution at pH 9.8. The extracted complexes showed maximum absorbance at 525 and 515 nm for methylene blue and safranine O, respectively. Results: In both the methods, the calibration curve was linear from 1-15 μg mL-1 of drug. Apparent molar absorpitivities were 1.7911 × 105, 2.3272 × 105 L mol-1 cm-1; Sandell's sensitivities were 0.0215, 0.0162 μg cm-2; LOD were 0.182, 0.175 μg mL-1; LOQ were 0.551, 0.531 μg mL-1 for methods MB and SO, respectively. The relative standard deviation and percent recovery ranged from 0.206-1.310% and 99.0-101.5%, respectively. Conclusion: The results demonstrate that the proposed methods are sensitive, precise, accurate and inexpensive. These methods can easily be used for the assay of ABS in quality control laboratories. © 2013 by Tabriz University of Medical Sciences.

Sridevi N.,Sri Krishna College of Engineering And Technology | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
Der Pharmacia Lettre | Year: 2012

A method for spectrophotometric determination of perindopril erbumine (PDE) in bulk and tablet dosage forms has been described. This method is based on the PDE ability with bromophenol blue (BPB), in acidic media, to form an ionic association extractible in chloroform. The absorbance of PDE-BPB ion pair complex is monitored spectrophotometrically at 425 nm and it is related to the concentration of PDE present. The method is optimized for acidity, concentration of BPB and solvent for the extraction required. The range of linearity for ion pair complex was found to be 5-125 μg mL -1 of PDE with molar absorptivity 5.210 x103 L mol -1cm -1 and sandell's sensitivity 0.0847 μg cm -2. The detection limit and quantitation limit of the PDE determination are found to be 0.256 μg mL -1 and 0.775 μg mL -1, respectively. The proposed method has been successfully applied to the determination of PDE in tablet dosage forms.

Lavudu P.,Vishnu Institute of Pharmaceutical Education and Research | Rani A.P.,Acharya Nagarjuna University | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
Chiang Mai Journal of Science | Year: 2016

A simple and rapid liquid chromatographic method was developed and validated for the determination of cilazapril (CZP) in bulk drug and pharmaceutical dosage form. Chromatographic separation has been achieved within 10 minutes by using an Cyano column (150 mm × 4.6 mm I.D., 5 μm particle size) as the stationary phase with a mobile phase consisted of formate buffer pH 3.75 and methanol (50:50 v/v) at a flow rate of 1.2 mL/min. Detection was performed at 227 nm using an ultraviolet detector. The method was validated in accordance with International Conference on Harmonization guidelines with respect to linearity, sensitivity, selectivity, accuracy, precision and robustness. Regression analysis showed good correlations (R2=0.9995) for CZP in the concentration range of 2-200 μg/mL. The percentage recovery was in the range of 99.20%-99.96% for CZP from the pharmaceutical dosage form. The proposed method is suitable for determination of CZP in pharmaceutical dosage form and bulk drug. © 2016, Chiang Mai University. All rights reserved.

Shanmukha Kumar J.V.,Koneru Lakshmaiah University | Prasanthi S.,Koneru Lakshmaiah University | Guravaiah M.,Jagarlamudi Kuppuswamy Choudary College | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

A new simple, sensitive, accurate, precise, selective and robust spectrophotometric method for the determination of oseltamivir phosphate in pure and in capsule dosage form is described. The method is based on the oxidation of the drug with alkaline potassium permanganate at room temperature. The absorbance of the green colored manganate ions produced is measured at 635 nm. All the experimental variables affecting the development of the manganate ions were investigated and the conditions were optimized. The calibration graph is linear in the range of 2-20 μg/ml with an excellent regression coefficient (R2 = 0.9984). The apparent molar absorptivity and Sandell's sensitivity values are 3.980 × 104 L/mole/cm and 0.0103 μg/cm2, respectively. The Limit of detection was 0.185 μg/ml and the limit of quantification was 0.564 μg/ml. The method has been successfully applied to the determination of drug in capsule dosage forms. Good recoveries were obtained. No interferences were observed from the common excipients in the capsule dosage forms. Results of the proposed method compare very favorably with those given by the reported UV spectrophotometric method. The accuracy and validity of the method were established by recovery studies via standard addition technique.

Madhavi N.,Jagarlamudi Kuppuswamy Choudary College | Deva Saroja T.,Jagarlamudi Kuppuswamy Choudary College
International Journal of Pharma and Bio Sciences | Year: 2014

The commercially available oil samples are analyzed for their Iodine, Saponification, Peroxide and Acid values. These values are subjected to statistical analysis by comparing with each other. The least Iodine value was identified for Coconut oil. Lower peroxide values were observed in the oils from Groundnut, Olive, Coconut and Rice bran. Sunflower oil exhibited the highest saponification value. Rice bran and Palm oil has the lowest Acid values. These properties help the chemist to opt for the suitable oil for household purposes and other requirements.

Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College | Bind V.H.,Jagarlamudi Kuppuswamy Choudary College | Damayanthi M.R.,Jagarlamudi Kuppuswamy Choudary College | Sireesha A.,Jagarlamudi Kuppuswamy Choudary College
Der Pharma Chemica | Year: 2013

A new, less time consuming and cost effective UV spectrophotometric method was developed for the quantification of rivaroxaban in bulk. Rivaroxaban was estimated at 270 nm in dimethyl sulphoxide. The linearity range was found to be 2-20μg mL-1 (regression equation: Absorbance = 0.1086 × Concentration of drug in μg mL-1 + 0.0154; R2 = 0.9991). The apparent molar absorptivity and sandell's sensitivity were found to be 4.825 × 104 L mol-1 cm-1 and 2.262 × 10-3 μg cm-2, respectively. The method was tested and validated for various parameters according to ICH guidelines. The results demonstrated that the procedure is sensitive, selective, precise, accurate, robust, rugged and useful for the determination of rivaroxaban in bulk samples.

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