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Madhavi V.,Tellakula Jalayya Polisetty Somasundaram College | Panchakshari V.,Jagarlamudi Kuppuswamy Choudary College | Prathyusha T.N.,Jagarlamudi Kuppuswamy Choudary College | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
International Journal of Pharmaceutical Sciences Review and Research | Year: 2011

A novel spectrophotometric method for the assay of mesalazine in bulk and dosage form has been developed. The method is based on the diazotization of mesalazine followed by its coupling with resorcinol to form a red colored azo dye. The colored product formed absorbed visible region strongly and optimal detector response was obtained at a wavelength of 490 nm. The analytical parameters and their effects on the reported method are investigated. Beer's law was obeyed in the concentration range of 2-20 μg/ml. The proposed method was directly and easily applied to the tablet dosage forms. Results obtained from the analysis of commercial preparations with the proposed method are in good agreement with those obtained with the reported spectrophotometric method.

Prathyusha T.N.,Jagarlamudi Kuppuswamy Choudary College | Panchakshari V.,Jagarlamudi Kuppuswamy Choudary College | Guravaiah M.,Jagarlamudi Kuppuswamy Choudary College | Sridevi N.,Siva Ramakrishna Institute of Technology | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
International Journal of Phytomedicine | Year: 2013

Three solvent extracts (methanol, ethanol and acetone) of Grewia orientalis leaves were screened for potential antibacterial activity against Escherichia coii, Bacillus substilis, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus thuringienis. The agar disk diffusion method using filter paper disks was used to study the antibacterial activity of Grewia orientalis leaf extracts against 6 microbial strains. All the three extracts did not exert any inhibitory action on Bacillus thuringienis. The highest antibacterial potentials were observed for the acetone extract, followed by methanol and ethanol extracts. The Minimum Inhibitory Concentration (MIC) of the plant extracts were ranging from 50 to 250 μg/ml. The preliminary phytochemical screening of extracts was carried out for major phytochemical derivatives in Grewia orientalis.

Lavudu P.,Vishnu Institute of Pharmaceutical Education and Research | Rani A.P.,Acharya Nagarjuna University | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
Chiang Mai Journal of Science | Year: 2016

A simple and rapid liquid chromatographic method was developed and validated for the determination of cilazapril (CZP) in bulk drug and pharmaceutical dosage form. Chromatographic separation has been achieved within 10 minutes by using an Cyano column (150 mm × 4.6 mm I.D., 5 μm particle size) as the stationary phase with a mobile phase consisted of formate buffer pH 3.75 and methanol (50:50 v/v) at a flow rate of 1.2 mL/min. Detection was performed at 227 nm using an ultraviolet detector. The method was validated in accordance with International Conference on Harmonization guidelines with respect to linearity, sensitivity, selectivity, accuracy, precision and robustness. Regression analysis showed good correlations (R2=0.9995) for CZP in the concentration range of 2-200 μg/mL. The percentage recovery was in the range of 99.20%-99.96% for CZP from the pharmaceutical dosage form. The proposed method is suitable for determination of CZP in pharmaceutical dosage form and bulk drug. © 2016, Chiang Mai University. All rights reserved.

Sri P.U.,Acharya Nagarjuna University | Kranthi G.,Jagarlamudi Kuppuswamy Choudary College | Rajasekhar P.,Acharya Nagarjuna University
Journal of Pure and Applied Microbiology | Year: 2010

The objective of the present study was to identify the cellulose degrading Trichoderma viride. Dilution plating was used to quantify the propagule numbers of Trichoderma, denaturing gradient gel electrophoresis (DGGE) and DNA sequence analysis were used to identify Trichoderma species. Trichoderma viride was identified based on the microscopic observations by Lactophenol cotton blue and scanning electron microscopy. It was ultimately confirmed based on DGGE and DNA sequence analysis and finally concluded by BLAST analysis by constructing a phylogenetic tree.

Shanmukha Kumar J.V.,Koneru Lakshmaiah University | Prasanthi S.,Koneru Lakshmaiah University | Guravaiah M.,Jagarlamudi Kuppuswamy Choudary College | Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

A new simple, sensitive, accurate, precise, selective and robust spectrophotometric method for the determination of oseltamivir phosphate in pure and in capsule dosage form is described. The method is based on the oxidation of the drug with alkaline potassium permanganate at room temperature. The absorbance of the green colored manganate ions produced is measured at 635 nm. All the experimental variables affecting the development of the manganate ions were investigated and the conditions were optimized. The calibration graph is linear in the range of 2-20 μg/ml with an excellent regression coefficient (R2 = 0.9984). The apparent molar absorptivity and Sandell's sensitivity values are 3.980 × 104 L/mole/cm and 0.0103 μg/cm2, respectively. The Limit of detection was 0.185 μg/ml and the limit of quantification was 0.564 μg/ml. The method has been successfully applied to the determination of drug in capsule dosage forms. Good recoveries were obtained. No interferences were observed from the common excipients in the capsule dosage forms. Results of the proposed method compare very favorably with those given by the reported UV spectrophotometric method. The accuracy and validity of the method were established by recovery studies via standard addition technique.

Madhavi N.,Jagarlamudi Kuppuswamy Choudary College | Deva Saroja T.,Jagarlamudi Kuppuswamy Choudary College
International Journal of Pharma and Bio Sciences | Year: 2014

The commercially available oil samples are analyzed for their Iodine, Saponification, Peroxide and Acid values. These values are subjected to statistical analysis by comparing with each other. The least Iodine value was identified for Coconut oil. Lower peroxide values were observed in the oils from Groundnut, Olive, Coconut and Rice bran. Sunflower oil exhibited the highest saponification value. Rice bran and Palm oil has the lowest Acid values. These properties help the chemist to opt for the suitable oil for household purposes and other requirements.

Sekaran C.B.,Jagarlamudi Kuppuswamy Choudary College | Bind V.H.,Jagarlamudi Kuppuswamy Choudary College | Damayanthi M.R.,Jagarlamudi Kuppuswamy Choudary College | Sireesha A.,Jagarlamudi Kuppuswamy Choudary College
Der Pharma Chemica | Year: 2013

A new, less time consuming and cost effective UV spectrophotometric method was developed for the quantification of rivaroxaban in bulk. Rivaroxaban was estimated at 270 nm in dimethyl sulphoxide. The linearity range was found to be 2-20μg mL-1 (regression equation: Absorbance = 0.1086 × Concentration of drug in μg mL-1 + 0.0154; R2 = 0.9991). The apparent molar absorptivity and sandell's sensitivity were found to be 4.825 × 104 L mol-1 cm-1 and 2.262 × 10-3 μg cm-2, respectively. The method was tested and validated for various parameters according to ICH guidelines. The results demonstrated that the procedure is sensitive, selective, precise, accurate, robust, rugged and useful for the determination of rivaroxaban in bulk samples.

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