Wilkinson S.,University of Arizona |
Johnson D.B.F.,Jack irball Center For Chemical Biology And Proteomics |
Tardif H.L.,University of Arizona |
Tome M.E.,University of Arizona |
Briehl M.M.,University of Arizona
Oncology Letters | Year: 2010
Mitochondria are central to a variety of cellular processes, from metabolism to cell death. In this study, we demonstrated that an increase in the critical mitochondrial protein, cytochrome c, correlated with drug resistance in a cell culture model of aggressive lymphoma. Increased cytochrome c expression was also correlated with decreased survival in the aggressive diffuse large B-cell and mantle cell lymphomas, but not in the indolent follicular lymphoma. This suggests that an increased reliance on the mitochondria for energy allows tumor cells to be metabolic opportunists and contribute to tumor development and drug resistance.
Takimoto J.K.,Jack irball Center For Chemical Biology And Proteomics |
Dellas N.,Jack irball Center For Chemical Biology And Proteomics |
Noel J.P.,Jack irball Center For Chemical Biology And Proteomics |
Noel J.P.,Salk Institute for Biological Studies |
Wang L.,Jack irball Center For Chemical Biology And Proteomics
ACS Chemical Biology | Year: 2011
Unnatural amino acids (Uaas) can be translationally incorporated into proteins in vivo using evolved tRNA/aminoacyl-tRNA synthetase (RS) pairs, affording chemistries inaccessible when restricted to the 20 natural amino acids. To date, most evolved RSs aminoacylate Uaas chemically similar to the native substrate of the wild-type RS; these conservative changes limit the scope of Uaa applications. Here, we adapt Methanosarcina mazei PylRS to charge a noticeably disparate Uaa, O-methyl-l-tyrosine (Ome). In addition, the 1.75 Å X-ray crystal structure of the evolved PylRS complexed with Ome and a non-hydrolyzable ATP analogue reveals the stereochemical determinants for substrate selection. Catalytically synergistic active site mutations remodel the substrate-binding cavity, providing a shortened but wider active site. In particular, mutation of Asn346, a residue critical for specific selection and turnover of the Pyl chemical core, accommodates different side chains while the central role of Asn346 in aminoacylation is rescued through compensatory hydrogen bonding provided by A302T. This multifaceted analysis provides a new starting point for engineering PylRS to aminoacylate a significantly more diverse selection of Uaas than previously anticipated. © 2011 American Chemical Society.