Bolton, MA, United States
Bolton, MA, United States

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Kozak D.,Izon Science U.S. Ltd. | Broom M.,Izon Science | Vogel R.,University of Queensland
Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 | Year: 2013

Tunable resistive pulse sensing (TRPS) represents a new and more comprehensive technology for measuring the properties of biological and synthetic particles. Based on particle-by-particle analysis, TRPS enables the accurate measurement of particle size, charge, and concentration to be made from a single analysis run. Herin we demonstrate the ananlysis capabilites of TRPS on liposomal particles. In addition to measuring concentration and volume fraction, TRPS is a highly sensitive size analysis technique, which is critical for liposome manufacture, formulation and delivery applications. The size distribution of liposomes formed by extrusion through two Nucleopore filter membranes, as well as the aggregation caused by freeze-thawing is shown. Finally we present the newly developed TRPS feature of particle-by-particle size and charge (zeta-potential) analysis for characterizing liposome surface modification (e.g. PEGylation), making TRPS a very accurate and comprehensive liposome and nanoparticle analysis tool.


Kozak D.,Izon Science U.S. Ltd | Broom M.,Izon Science | Vogel R.,Izon Science | Vogel R.,University of Queensland
Current Drug Delivery | Year: 2015

The pharmaceutical industry as well as European and US governing agencies have indicated the need for more accurate, high resolution, characterization of complex drug materials, nanomedicines, to facilitate their development and eventual approval. In particular, accurately measuring the size, zeta-potential, and concentration of nanomedicines is desired. Herein we demonstrate the comprehensive and high resolution analysis capabilities of tunable resistive pulse sensing (TRPS) on the most widely approved nanomedicines to-date, liposomal particles. The number-based size distribution, concentration and volume fraction of liposomes formed by extrusion through a 100 nm or 200 nm Nucleopore filter membrane are shown as well as how freeze-thaw aggregation changes individual liposomes and the overall size distribution. In addition, the simultaneous size and zeta-potential analysis capabilities of TRPS is used to characterize the homogeneity and difference between liposomes made with and without the addition of PEGylated phospholipids. © 2015 Bentham Science Publishers.


PubMed | Izon Science U.S. Ltd
Type: Journal Article | Journal: Current drug delivery | Year: 2015

The pharmaceutical industry as well as European and US governing agencies have indicated the need for more accurate, high resolution, characterization of complex drug materials, nanomedicines, to facilitate their development and eventual approval. In particular, accurately measuring the size, zeta-potential, and concentration of nanomedicines is desired. Herein we demonstrate the comprehensive and high resolution analysis capabilities of tunable resistive pulse sensing (TRPS) on the most widely approved nanomedicines to-date, liposomal particles. The number-based size distribution, concentration and volume fraction of liposomes formed by extrusion through a 100 nm or 200 nm Nucleopore filter membrane are shown as well as how freeze-thaw aggregation changes individual liposomes and the overall size distribution. In addition, the simultaneous size and zeta-potential analysis capabilities of TRPS is used to characterize the homogeneity and difference between liposomes made with and without the addition of PEGylated phospholipids.

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