Iwate Industrial Research Institute

Morioka, Japan

Iwate Industrial Research Institute

Morioka, Japan
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Tamakawa H.,Iwate Industrial Research Institute | Ito Y.,Iwate Industrial Research Institute
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2017

The 16S rDNA genotypes among lactic acid bacteria were determined using PCR-RFLP analysis. Eight tetrameric restriction enzymes (AccII, AfaI, HaeIII, HhaI, MboI, MspI, TaqI, XspI) were used for RFLP analysis and adequate numbers of informative band were obtained from each enzyme. Eighty-Two genotypes were obtained from 99 lactic acid bacteria. In the case of only two restriction enzymes, sixty-five genotypes, which are most in all combination, were obtained from the combination of XspI and MspI restriction patterns. Therein, fifty-Two species could be assigned to specific genotype, supporting the application of this analysis for identification of bacterial species. The genotype assigned by PCR-RFLP using XspI and MspI showed 92.3% successful phylogenetic affiliations. PCR-RFLP analysis using 20 lactic acid bacteria was demonstrated, and the results corresponded approximately to the calculated fragment patterns. These results indicate that PCR-RFLP should be useful for genotyping, identification and approximately classification of lactic acid bacteria. Copyright © 2017, Japanese Society for Food Science and Technology.


Sugimura S.,Tokyo Denpa Co. | Endo H.,Iwate Industrial Research Institute | Kashiwaba Y.,Iwate Industrial Research Institute | Kashiwaba Y.,Iwate University | Sato E.,Iwate Medical University
Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment | Year: 2011

We evaluated photoluminescence, radioluminescence, transmittance, and decay time at room temperature at each sector of zinc oxide (ZnO) single crystals grown utilizing the hydrothermal process. The -c-sector wafer, grown on the oxygen face of the c-plane seed crystal, has a short decay time and a high light yield and shows properties different from those of other sectors. We also fabricated a radiation sensor by combining the -c-sector chip cut from a wafer and a multipixel photon counter (MPPC). X-rays were detected using sensor with high sensitivity. At room temperature, typical decay time for the -c sector at the near band edge was 700 ps, and the range of relative light yield was thirty- to forty-times larger than that of the c sector. Therefore, the properties of the -c-sector wafer at the near band edge will be suitable for high-speed radiation sensors in the near future. © 2011 Elsevier B.V. All right reserved.


Yano A.,Iwate Biotechnology Research Center | Kikuchi S.,Iwate Biotechnology Research Center | Takahashi T.,Iwate Industrial Research Institute | Kohama K.,Iwate Industrial Research Institute | And 2 more authors.
Archives of Oral Biology | Year: 2012

Objectives: The anti-cariogenic properties of the phenolic fraction from the pomace of Vitis coignetiae (VcPP) were evaluated by in vitro assays and compared with fruit juices from V. coignetiae and common grapes and with other phenolic fractions. The effects of VcPP against the biofilm of Streptococcus mutans were investigated. Design: Sucrose-dependent biofilm formation by S. mutans cultured in the presence of VcPP was measured by crystal violet dye uptake. Inhibition of adhesion to the saliva-coated hydroxyapatite (sHA) beads was quantified using fluorescent-labelled cells. The MIC for S. mutans was determined by colony counting on agar plates containing VcPP. The ability of VcPP to inhibit glucan synthesis by three distinct recombinant glucosyltransferases (Gtfs) was assessed by quantifying the production of water-soluble and -insoluble polysaccharides in bacterial cultures. In addition, the buffering effect of VcPP in cultures of S. mutans was evaluated. Results: VcPP reduced adhesion of S. mutans to sHA and biofilm formation in a dose-dependent manner. The MIC of VcPP was 7.50 mg/ml. VcPP inhibited GtfB activity associated with the synthesis of water-insoluble glucans. It also inhibited GtfD activity associated with the synthesis of water-soluble glucans at a concentration which was lower than that used for inhibition of GtfB. VcPP had no effect on acidification associated with glucose utilization by S. mutans. Conclusions: The current study supports the potential of VcPP as a food additive for reducing caries by inhibiting adhesion to the tooth surface and GtfD-mediated soluble glucan synthesis. © 2012 Elsevier Ltd. All rights reserved.


Sato E.,Iwate Medical University | Sugimura S.,Tokyo Denpa Co. | Endo H.,Iwate Industrial Research Institute | Oda Y.,Iwate Medical University | And 10 more authors.
Applied Radiation and Isotopes | Year: 2012

15. Mcps photon-counting X-ray computed tomography (CT) system is a first-generation type and consists of an X-ray generator, a turntable, a translation stage, a two-stage controller, a detector consisting of a 2. mm-thick zinc-oxide (ZnO) single-crystal scintillator and an MPPC (multipixel photon counter) module, a counter card (CC), and a personal computer (PC). High-speed photon counting was carried out using the detector in the X-ray CT system. The maximum count rate was 15. Mcps (mega counts per second) at a tube voltage of 100. kV and a tube current of 1.95. mA. Tomography is accomplished by repeated translations and rotations of an object, and projection curves of the object are obtained by the translation. The pulses of the event signal from the module are counted by the CC in conjunction with the PC. The minimum exposure time for obtaining a tomogram was 15. min, and photon-counting CT was accomplished using gadolinium-based contrast media. © 2011 Elsevier Ltd.


Sun S.-H.,Tohoku University | Koizumi Y.,Tohoku University | Kurosu S.,Iwate Industrial Research Institute | Li Y.-P.,Tohoku University | Chiba A.,Tohoku University
Acta Materialia | Year: 2015

Co-28Cr-6Mo-0.23C-0.17N alloy cylindrical rods were fabricated by electron beam melting (EBM) with cylindrical axes along the build direction. The inhomogeneity in microstructures of the as-fabricated rods and heat-treated rods were investigated, along with the creep behavior of the heat-treated rods, focusing on the influence of microstructural inhomogeneity. Although the constituent phase varied along the build direction in the as-EBM-built rod, from single ε-hexagonal close-packed (hcp) phase in the bottom to single γ-face-centered cubic (fcc) phase in the top, the γ-fcc phase can be kept in a wide range of build height, i.e. ∼40 mm from the top finishing plane. The as-EBM-built rods consisting of both ε phase and γ phase can be transformed into single ε-hcp phase by the aging treatment at 800 °C for 24 h. However, the ε-hcp grain size in the aged rod was heterogeneous along the build height. The grain size increased along the build height at first, then decreased gradually to the position where the phase transitioned from γ-fcc to ε-hcp in the as-EBM-built rod. The grain size was nearly uniform on the top part, which used to be single γ-fcc phase. The values of stress exponent n and apparent activation energy Q were determined to be 5.0 and 365 kJ mol-1, respectively. Intergranular fracture occurred, and fracture nearly always occurred in the homogeneous fine-grain region. The decrease in stacking fault energy with increasing temperature keeps the dislocations expanding and increases the apparent activation energy. © 2014 Acta Materialia Inc.


Belmoubarik M.,Tohoku University | Nozaki T.,Tohoku University | Endo H.,Iwate Industrial Research Institute | Sahashi M.,Tohoku University
Journal of Applied Physics | Year: 2013

Deposition of ZnO thin films on a ferromagnetic metallic buffer layer (Co3Pt) by molecular beam epitaxy technique was investigated for realization of ZnO-based magnetic tunneling junctions with good quality hexagonal ZnO films as tunnel barriers. For substrate temperature of 600 °C, ZnO films exhibited low oxygen defects and high electrical resistivity of 130 Ω cm. This value exceeded that of hexagonal ZnO films grown by sputtering technique, which are used as tunnel barriers in ZnO-MTJs. Also, the effect of oxygen flow during deposition on epitaxial growth conditions and Co 3Pt surface oxidation was discussed. © 2013 American Institute of Physics.


Iimura T.,Iwate Industrial Research Institute | Iyama T.,Iwate University | Inoue K.,Tokosha CO.
Proceedings of the 13th International Conference of the European Society for Precision Engineering and Nanotechnology, EUSPEN 2013 | Year: 2013

The distinctive structure of precision scissors causes lower vertical direction rigidity in closing motion. As a result, when a load necessary to cut a workpiece by impressing it is heavy, the "Sandwiching" state occurs. The "Sandwiching" state is the state that a workpiece jams into an opened space between two blades of precision scissors, and the precision scissors cannot cut the workpiece. In this paper, we analysed the mechanism of the "Sandwiching" state and present the effects of the vertical load of the blades on the "Sandwiching" state. The result shows that the "Sandwiching" state can be controlled by the vertical load.


Thwe S.M.,Tokyo University of Marine Science and Technology | Kobayashi T.,Tokyo University of Marine Science and Technology | Luan T.,Tokyo University of Marine Science and Technology | Shirai T.,Tokyo University of Marine Science and Technology | And 3 more authors.
Fisheries Science | Year: 2011

γ-Aminobutyric acid (GABA)-producing lactic acid bacteria (LAB) were isolated from four types of Myanmar traditional fermented fishery products with boiled rice. All of them belonged to the genus Lactobacillus, and comparison of the effects of these representatives on GABA accumulation in fermented fishery products with boiled rice revealed that Lactobacillus farciminis D323 is the most effective strain as a starter culture. These results may contribute to the development of traditional fermented fishery products with functional properties. In addition, this study is the first to show in detail the distribution of GABA-producing LAB in Southeast Asian fermented fishery products. © 2011 The Japanese Society of Fisheries Science.


Ye R.,Iwate University | Baba M.,Iwate University | Ohta K.,Iwate University | Suzuki K.,Iwate Industrial Research Institute
Solid-State Electronics | Year: 2010

We report the effects of thermal annealing on structure, morphology and electrical properties of fluorinated copper phthalocyanine (F 16CuPc)/α-sexithiophene (α6T) heterojunction thin films. The morphology and structure of the thin films were examined by atomic force microscopy and X-ray diffraction techniques. The thermal annealing significantly improved α6T molecular ordering although F16CuPc molecular was slightly disordered in the thermal annealing process. The smallest full width of half maximum of the 200 diffraction lines was gained at the thermal annealing temperature of 150 °C. Similarly, ambipolar performance of the F 16CuPc/α6T heterojunction transistor could be improved by the thermal annealing. At the thermal annealed temperature of 150 °C, the ambipolar device achieved high-performance with field-effect hole and electron mobilities of 8.84 × 10-3 cm2 V-1 s -1 and 1.00 × 10-2 cm2 V-1 s-1, respectively. © 2010 Elsevier Ltd. All rights reserved.


Meng Q.,Iwate University | Hatakeyama M.,Iwate Industrial Research Institute | Sugawara E.,Iwate University
Bioscience, Biotechnology and Biochemistry | Year: 2014

Two aroma compounds of volatile thiols, 2-furanmethanethiol (2FM) and ethyl 2-mercaptopropionate (ET2MP), were formed in five types of Japanese soy sauce during fermentation by yeast. The concentrations of 2FM and ET2MP in the soy sauce samples increased during alcoholic fermentation. The concentrations of 2FM and ET2MP were higher in the soy sauce fermented by Zygosaccharomyces rouxii than in that fermented by Candida versatilis. The enantiomers of ET2MP were separated by gas chromatography in a capillary column. The average enantiomeric ratio of ET2MP in the soy sauce was approximately 1:1. 2FM was formed by yeast in a medium prepared from cysteine and furfural, and cysteine is considered the key precursor of 2FM by yeast in soy sauce. © 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry.

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