Iwate Agricultural Research Center Animal Industry Research Institute

Morioka-shi, Japan

Iwate Agricultural Research Center Animal Industry Research Institute

Morioka-shi, Japan

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Watanabe N.,Oita Prefectural Institute of Animal Industry | Yamada T.,Kyoto University | Yoshioka S.,Beef Information and Genetics Institute Inc. | Itoh M.,Oita Prefectural Institute of Animal Industry | And 6 more authors.
Animal Science Journal | Year: 2010

Our previous study detected a single nucleotide polymorphism (SNP), g.1471620G>. T, in the 5′ flanking region of the endothelial differentiation sphingolipid G-protein-coupled receptor 1 (EDG1) gene, which has been considered as a positional functional candidate for the gene responsible for marbling, and showed association of the g.1471620G>. T SNP with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the g.1471620G>. T SNP among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Short Horn, Holstein, and Brown Swiss breeds. The T allele at the g.1471620G>. T SNP associated with high marbling was found at high frequency in Japanese Black breed that has been subjected to a strong selection for high marbling, while the allele was absent or at very low frequencies in the other breeds that have not been strongly selected for high marbling. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequency of the T allele at the g.1471620G>. T SNP in the EDG1. © 2009 The Authors. Journal compilation © 2009 Japanese Society of Animal Science.


Tohno M.,Japan National Agriculture and Food Research Organization | Kitahara M.,RIKEN | Irisawa T.,RIKEN | Masuda T.,Iwate Agricultural Research Center Animal Industry Research Institute | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

A Gram-reaction-positive, facultatively anaerobic, non-spore-forming and catalase-negative rod-shaped bacterial strain, designated IWT126T, was isolated from orchardgrass (Dactylis glomerata L.) silage preserved in Hachimantai, Iwate, Japan. The isolate showed growth at 15-45 °C, pH 3.5-7.5 and with 4.0 % (w/v) NaCl. The cell wall peptidoglycan did not contain meso-diaminopimelic acid, and the DNA G+C content was 45.6 mol%. The major cellular fatty acids were C16: 0 and C19: 1 cyclo 9,10. Based on 16S rRNA gene sequence similarity, strain IWT126T was classified as a member of the genus Lactobacillus and was most closely related to Lactobacillus odoratitofui YIT 11304T (98.7 %), Lactobacillus similis JCM 2765T (98.5 %), Lactobacillus collinoides JCM 1123T (97.6 %), Lactobacillus paracollinoides DSM 15502T (97.6 %) and Lactobacillus kimchicus DCY51T (96.9 %). Based on sequence analysis of the phenylalanyl-tRNA synthase α-subunit (pheS) gene, strain IWT126T was well separated from its phylogenetic neighbours in the genus Lactobacillus. Based on physiological, biochemical and genotypic results, as well as low DNA-DNA relatedness to recognized phylogenetic relatives in the genus Lactobacillus, classification of strain IWT126T as a representive of a novel species named Lactobacillus silagei sp. nov. is proposed. The type strain is IWT126T (= JCM 19001T = DSM 27022T). © 2013 IUMS.


Tohno M.,Japan National Agriculture and Food Research Organization | Kitahara M.,RIKEN | Irisawa T.,RIKEN | Ohmori H.,Japan National Agriculture and Food Research Organization | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

Using a polyphasic taxonomic approach, we investigated three bacterial strains – IWT30T, IWT8 and IWT75 – isolated from total mixed ration silage prepared in Hachimantai, Iwate, Japan. The isolates comprised Gram-stain positive, non-motile, non-spore-forming, catalasenegative, rod-shaped bacteria. Good growth occurred at 15–45°C and at pH 4.0–7.5 Their major cellular fatty acids were C18:1ω9c and C19:1 cyclo 9,10.The G+C content of genomic DNA of strain IWT30T was 44.6 mol%. Comparative 16S rRNA gene sequence analysis showed that these novel strains belonged to the genus Lactobacillus. These strains shared 100% 16S rRNA gene sequence similarity and were most closely related to the type strains of Lactobacillus silagei, Lactobacillus odoratitofui, Lactobacillus similis, Lactobacillus collinoides, Lactobacillus paracollinoides and Lactobacillus kimchicus, with sequence similarity values of 99.5, 98.8, 98.7, 97.8, 97.8 and 96.8 %, respectively. The level of DNA–DNA relatedness between these strains and their closest phylogenetic neighbours was less than 30 %. On the basis of additional phylogenetic analysis of pheS and rpoA gene sequences and phenotypic and chemotaxonomic characteristics, we conclude that these three strains represent a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus mixtipabuli sp. nov. The type strain is IWT30T (=JCM 19805T=DSM 28580T). © 2015, IUMS.

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