Venkatesh G.,ICAR National Institute of High Security Animal Diseases |
Vanamayya P.R.,IVRI Campus |
Sharma N.,ICAR National Institute of High Security Animal Diseases |
Kulkarni D.D.,ICAR National Institute of High Security Animal Diseases |
Dubey S.C.,ICAR National Institute of High Security Animal Diseases
Journal of Pure and Applied Microbiology | Year: 2015
A gene fragment (1008bp) of Porcine Parvovirus (PPV) non-structural protein NS1 was amplified by PCR from tonsillar tissue sample. The amplicon was cloned and sequenced. The deduced amino acid sequences of the gene fragment showed more than 98% homology with published sequences. The NS1 gene fragment was subcloned into prokaryotic expression vector pET28a (+) and designated as pET-NS1. The recombinant plasmid was transformed into E.coli BL21(DE3) pLysS cells and the expression of the truncated recombinant NS1 (rNS1) protein with a size of 43kDa was induced with ImM IPTG for four hours. The rNS1 protein was purified by affinity column chromatography under denaturing conditions and characterised by SDS-PAGE and Western blot. It reacted with PPV positive serum but not with PPV negative serum or Porcine Circovirus serum. The rNS1 protein can be used to develop tests to detect PPV antibodies in infected animals and also to differentiate it from animals vaccinated with inactivated vaccine as it is found only in virus-infected cells but not in mature virion.
Bhattacharyya A.,Central Avian Research Institute |
Majumdar S.,IVRI Campus |
Majumdar S.,Central Avian Research Institute |
Bhanja S.K.,IVRI Campus |
And 5 more authors.
Indian Journal of Animal Sciences | Year: 2013
An experiment was designed involving maternal vaccination (MV or no MV), in ovo vaccination (IV or no IV) and conventional vaccination (CV or no CV). 7 days post ND (Newcastle disease) vaccination, 460 fertile eggs were collected and on 25th embryonic day (ED), 230 eggs were in ovo vaccinated with formaldehyde inactivated ND F1 vaccine. After hatch, half of the turkey chicks from respective group were conventionally vaccinated and reared. The group having maternal vaccination and in ovo vaccination had higher hatchability. Haemagglutination inhibition (HI) titer (log2) against ND vaccination was significantly higher in poults hatched from maternally vaccinated hens than the un-vaccinated ones up to 28 days of age. The poults with in ovo vaccination, had higher titer than un-in ovo vaccinated poults till 49 days of age. Combination of maternal vaccination and in ovo vaccination had higher titre value than that in ovo vaccinated group till 21 days of age. Thereafter only in ovo vaccinated groups had higher titre than either maternal or combination of both. However, they had significantly higher titer values than unvaccinated poults. In ovo vaccination also had apparently higher titer values than conventional vaccination till 28 days of age. In ovo vaccination and conventional vaccination did not show any beneficial effect on HI titer. Only maternal vaccination maintained higher titer value than conventional vaccination up to 21 days of age. Thereafter, conventional vaccinated group had higher titer value. Combination of maternal vaccination and conventional vaccination did not elicit better immune response than either maternal or conventional vaccination. Hence, it may be concluded that in ovo vaccination did not affect the hatchability and combination of maternal and in ovo vaccination may elicit better antibody titer against ND vaccination in the progeny.
Rout M.,IVRI Campus |
Senapati M.R.,IVRI Campus |
Mohapatra J.K.,IVRI Campus |
Dash B.B.,IVRI Campus |
And 2 more authors.
Preventive Veterinary Medicine | Year: 2014
Serological investigation to detect foot-and-mouth disease (FMD) virus circulation in the domestic small ruminant population of India was conducted. A total of 4407 and 4035 serum samples from sheep and goats, respectively were collected at random covering majority of the states across the country during 2010-2012. These samples were analyzed for antibodies against the non-structural proteins (NSP) of FMD virus in an indirect 3AB NSP ELISA and against the structural proteins (SP) in a liquid phase blocking (LPB) ELISA. A total of 20.35% sheep and 13.60% goats were found to be positive for 3AB NSP antibodies providing a serological evidence of extensive viral activity. In LPB ELISA, only 4.54% sheep and 6.27% goats were found to have protective antibody (log10 titre of ≥1.8) against all three serotype strains in the vaccine, which correlates with "no or sparse vaccination" scenario in these species in the country. Hence, to check silent amplification and dissemination of virus in a mixed farming set up, small ruminants may be brought under the ambit of routine vaccination and surveillance programmes. © 2013 Elsevier B.V.