Ivano Frankivsk State Medical Academy

Ivano Frankivsk, Ukraine

Ivano Frankivsk State Medical Academy

Ivano Frankivsk, Ukraine

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Jastrebova O.,Ivano Frankivsk State Medical Academy | Kovalchuk I.,University of Lethbridge | Kovalchuk L.,Ivano Frankivsk State Medical Academy
Carpathian Journal of Earth and Environmental Sciences | Year: 2014

In this work we used Su/+ Nicotiana tabacum plants to analyze potential mutagenecity of soils from three different zones in Ukraine, zone of chemical contamination, zone of enhanced radiological control and conditionally clean zone. Leaves of Su/+ plants are pale green with occasional appearance of dark green spots indicating mutations at the Sulfur locus. We found that plants grown on soils from all three zones had shorter stem and smaller crown diameter as compared to plants grown on control greenhouse soil. For the mutagenecity assay, we analyzed the spot appearance, the number of spots per plant and the average area of spots per plant. The analysis showed that soil samples from most of the tested areas had higher mutagenecity as compared to control soil sample. Thus, our data showed that Su/+ plants are effective indicators of soil pollution.


Jastrebova O.,Ivano Frankivsk State Medical Academy | Kovalchuk I.,University of Lethbridge | Kovalchuk L.,Ivano Frankivsk State Medical Academy
Carpathian Journal of Earth and Environmental Sciences | Year: 2011

Soil contamination is the result of rapid urbanization. Since plants are used as food and feed, it is important to analyze the effect of soil contaminants on plant growth. Moreover, plants can also be used to detect genotoxic effects of potential mutagens. Here, we analyzed whether Nicotiana tabacum Su/+ plants that are heterozygous for the Sulfur gene can be used for biomonitoring of potential soil pollution. Leaves of these plants are pale green with occasional appearance of dark green spots. It was previously suggested that the appearance of these spots is the result of DNA damage at the Sulfur locus. Plants were germinated and grown on soil taken from five different areas of one of the Ukrainian cities, Ivano-Frankivsk. The areas were designated as clean (Plot #1 and Plot #2) and contaminated (Plot #3, 4 and 5). We analyzed the dynamics of stem elongation and changes in crown diameter. The analysis showed that plants grown on soil from Plot#5 had the slowest dynamics of both stem and crown growth. The dynamics of stem growth was statistically similar among other four samples. In contrast, the dynamics of crown growth was significantly slower in plants grown on soil from Plot#3 and #1, as compared to Plot#4 and #2. The analysis of spot appearance showed that the highest number of spots and the largest total area of spots were observed in plants from Plot#3. Analysis of index of mutation intensity (IMI) that reflects the percentage of leaf area occupied by mutation spots showed the lowest IMI in plants grown on soil from Plot#1 and 2 and the highest in plants from Plot #3, 4 and 5.

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