Ivano Frankivsk National Medical University

Ivano Frankivsk, Ukraine
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Kotyk T.,Ivano Frankivsk National Medical University
Microscopy Research and Technique | Year: 2016

The objective of study is to study the peculiarities of morphological changes in different subdivisions of the intralobular duct of the submandibular gland (SMG) in rats in case of experimental diabetes mellitus (DM). The study included sexually mature male Wistar rats. Experimental DM was induced by streptozotocin. Electron microscopic study of subdivisions of the intralobular duct of the SMG was carried out on the 14th, 28th, 42nd, 56th, and 70th days of the experiment. In early stages of experimental DM the intercalated ducts are characterized by a relatively unchanged structure, and in late stages vacuolization of the cytoplasm of their epithelial cells is observed. Since the 14th day vacuolization of mitochondria is observed in epithelial cells of the granular ducts being the most pronounced on the 28th day and not apparent over the subsequent periods. The degree of filling with granules reduces till 56th day, however, it increases sligthly on the last day of the experiment. On the 28th–70th days vacuolization of the cytoplasm is observed in epithelial cells of the striated ducts. In addition, on the 14th day the mitochondrial matrix of these cells condenses; over the next periods it becomes enlightened and mitochondrial cristae are clearly visualized and disorganized. Conclusion: In the intralobular duct of the SMG in experimental DM dystrophic changes of different intensity occur in the granular and striated ducts on the 14th day and in the intercalated ducts only since the 42nd day of the experiment. © 2016 Wiley Periodicals, Inc.

Moiseenko N.M.,Ivano Frankivsk National Medical University
Oftalmologicheskii Zhurnal | Year: 2015

Search of conditions conducive to the activation of regenerative processes and restoration of the functions of the optic nerve after traumatic injuries is relevant. The purpose of this research was to study morphological and functional aspects of the rehabilitation process in the optic nerve after traumatic injury under the influence of high doses of corticosteroids. Material and methods. 24 adult rabbits were used,12 of them were from the group of rabbits injured without treatment and another 12 were treated with methylprednisolone on the second day after the injury. Morphological study was conducted on the 14th day, and the functional one (the amplitude of pupillary reaction) was carried out on the 14th day and in 1 month. Results After the use of high doses of corticosteroids in the intracranial portion of the optic nerve, on the 24th day, improvement of microcirculation, decreasing swelling and remyelination of nerve fibers were detected. At the same time, the amplitude of the pupillary reaction has increased in one month. Conclusions. It was established that in case of traumatic injury, usage of high-dose corticosteroids contributes not only to morphological but also to functional signs of activation of regenerative processes of the optic nerve. © N.M. Moiseenko, 2015.

Zhurakovskaya O.Ya.,Ivano Frankivsk National Medical University
Neuroscience and Behavioral Physiology | Year: 2014

This study addresses the morphofunctional organization of the ventromedial nucleus (VMN) of the hypothalamus in rats of different ages with streptozotocin diabetes mellitus (DM). On day 7 of the development of experimental DM, three-month-old rats showed a stress reaction characterized by ultrastructural signs of increased functional activity of light neurosecretory cells (NSC) in the VMN of the hypothalamus, while NSC in the VMN in 24-month old rats remained intact. These changes in the VMN in DM in three-month-old animals are probably associated with high glucose levels. On day 28 of DM, animals of different age groups showed decreases in the number of NSC in the VMN due to apoptosis and hydropic dystrophy. These changes were accompanied by deterioration of NSC trophics due to the initial stages of development of diabetic microangiopathy and destructive changes to gliocytes. © 2014 Springer Science+Business Media New York.

Semianchuk V.B.,Ivano Frankivsk National Medical University
Wiadomosci lekarskie (Warsaw, Poland : 1960) | Year: 2017

INTRODUCTION: Periodic fever, aphthous stomatitis, pharyngitis and cervical adenitis (PFAPA) syndrome refers to a group of primary immunodeficiencies, namely autoinflammatory diseases. Most pediatricians and otolaryngologists do not suspect PFAPA syndrome when treating recurrent pharyngitis (according to Ukrainian classification - tonsillitis) and stomatitis. Therefore, patients with a given syndrome receive unnecessary treatment (antibiotic therapy or antiviral drugs) and the diagnosis is made late. The aim of the research was to provide pediatricians, family physicians and otolaryngologists with information on the importance of early diagnosis of PFAPA syndrome.MATERIALS AND METHODS: The analysis of the prevalence and diagnosis of PFAPA syndrome in Ukraine and worldwide has been made as well as a late diagnosis of PFAPA syndrome in a child living in Ivano-Frankivsk, Ukraine has been described (case report).RESULTS: The Сase report 7-year-old boy, who grows and develops normally. The symptoms of pharyngitis including high body temperature (>40 º С), sore throat and white spots on the tonsils appeared for the first time at the age of two years. The boy received antibacterial drugs about 10 times a year. During a four-year period of recurrent episodes of the disease antimicrobial susceptibility testing to determine susceptibility of the oropharyngeal flora to the antibiotics were continuously performed, different blood tests for herpes viruses, Epstein-Barr virus infection and cytomegalovirus in particular were made using the enzyme immunoassay (EIA) and polymerase chain reaction (PCR) in addition to long-term treatment.CONCLUSIONS: An example of late diagnosing PFAPA syndrome (four years after the onset of first symptoms) resulting in regular examinations, medical manoeuvres, outpatient and inpatient treatment, use of antibiotic therapy including intravenous injections on a monthly basis has been studied.

Mazurak N.,University of Tübingen | Mazurak N.,Ivano Frankivsk National Medical University | Seredyuk N.,Ivano Frankivsk National Medical University | Sauer H.,University of Tübingen | And 2 more authors.
Neurogastroenterology and Motility | Year: 2012

Background: Patients with irritable bowel syndrome (IBS) often present with disturbances of bowel habits (diarrhea, constipation) and abdominal pain/discomfort that are modulated by the autonomic nerve system (ANS). In this narrative review, we analyzed studies that measured ANS functioning in IBS by means of heart rate variability (HRV). Methods: The PUBMED was searched with the keywords 'irritable bowel syndrome' AND ('heart rate variability' OR 'autonomic function'). We included only papers that used 'traditional' HRV indices and diagnosed IBS based on Manning or Rome criteria. Studies were sub-grouped according to methodological features of HRV analysis (24-h monitoring, short-term laboratory records, records during sleep). Key Results: Most studies reported no difference in HRV when the IBS population was compared to healthy controls. Dividing the IBS sample into subgroups - according to their predominant bowel symptoms, the severity of clinical course, the presence of depressive symptoms, or a history of abuse in the past - revealed changes in autonomic functioning. Conclusions & Inferences: Patients with IBS appear to experience symptoms that may be the result of changes in ANS functioning. HRV measures in clinical routine may allow assessing these changes, but further studies performed in a standardized fashion should improve the validity of HRV measures for clinical research first. © 2012 Blackwell Publishing Ltd.

Parashchuk T.O.,Ivano Frankivsk National Medical University
Journal of Nano- and Electronic Physics | Year: 2016

The work is devoted to the theoretical calculations and experimental study of important thermodynamic parameters of II-VI crystals. Cubic sphalerite and hexagonal wurtzite cluster models of polymorphs for stoichiometric II-VI crystals (ZnX, CdX; X = S, Se, Te) have been proposed. In the work one carried out the calculations of thermodynamic parameters under normal conditions and defined the analytical expressions of temperature dependences for energy ΔE, enthalpy ΔH, Gibbs free energy ΔG, entropy ΔS for sphalerite phase stoichiometric crystals of zinc and cadmium chalcogenides. Using the results of DFT-calculations according to equality of Gibbs free energy for sphalerite and wurtzite phases (ΔGS = ΔGW) of zinc chalcogenides crystals we defined phase transition "sphalerite-wurtzite" temperatures, which decreased in the line ZnS (1454 K) - ZnSe (1427 K) - ZnTe (1382 K) and was found the specified region of stability for these polymorphs. © 2016 Sumy State University.

Pakharenko L.V.,Ivano Frankivsk National Medical University
New Armenian Medical Journal | Year: 2015

One of the most common neuroendocrine syndromes in gynecology is premenstrual syndrome. The article is devoted to the study of progesterone level and frequency of polymorphic variants T1T2 of progesterone receptor gene PROGINS, as well as their possible relationship in patients with various forms of PMS. The study involved 50 women of reproductive age with PMS diagnosis, including 25 women with edematous form of the disease, 25 - with neuropsychic form. Of the same 50 patients twenty five women had mild form of the disease, and 25 women had severe form. Control group consisted of 25 women without PMS diagnosis. Progesterone level was determined in blood serum twice in the first and second phase of a menstrual cycle. Polymorphism of T1T2 of PROGINS progesterone receptor gene was studied using polymerase chain reaction. We found hypoprogesteronemia of the luteal phase of a menstrual cycle in women with PMS, especially pronounced in persons with edematous and severe forms of the disease (p<0.05). Polymorphic variant T1T2 of PROGINS gene can be considered as a marker of PMS development. T1T1 genotype was significantly associated with development of edematous form (?2=4.50, ?=0.03) and its presence may indicate a tendency to develop into severe form of PMS (?2=3.21, ?=0.07). T2 allele can be considered as a protective mechanism for the appearance of PMS, particularly its edematous and severe forms, in which the presence of T2 allele reduces the risk of PMS, respectively, by 3.0 (?2=4.50, p=0.03, OR=0.20, 95% CI 0.05-0.78, p=0.02) and by 1.71 times (?2=3.21, p=0.07, OR=0.27, 95% CI 0.08-0.95, p=0.04) compared with the control group. Reduced blood level of progesterone in women with PMS correlated with the presence of T1T1 genotype of PROGINS gene. PMS patients with T1T2 genotype exhibited significantly low levels of this hormone in the second phase of menstrual cycle (p<0.05) compared to women with the same genotype in the control group. Furthermore, women with this neuroendocrine syndrome and T1T2 genotype showed no statistically significant difference in progesterone level in these groups compared with healthy women with the same genotype (p>0.05). © 2015, Yerevan State Medical University. All rights reseved.

Perederko L.P.,Ivano Frankivsk National Medical University
Zoology and Ecology | Year: 2014

The paper presents the results of investigation of the amount of biogenic nutrients in the blood of the African Ostrich (Struthio camelus) fed low mineral content diets in the temperate continental climate in the western region of Ukraine. Blood samples were taken from the wing vein. The serum of males of about two years of age was used for analysis. In the study area, Struthio camelus were bred in an intensive manner, where the birds were in limited areas with free access outside. The amount of minerals in the feed strongly affected the level of biogenic nutrients in blood. The study of haematological parameters of trace minerals in the blood of African Ostriches is of great diagnostic value in determining the internal changes and pathological diagnosis of defects in the organism. © 2014 Nature Research Centre.

Pakharenko L.,Ivano Frankivsk National Medical University
Georgian medical news | Year: 2014

To identify risks of development of any disease is a priority of modern medicine. The aim of this study was to investigate the frequency of polymorphic variants of A-351G gene estrogen receptor ESR1 in patients with various forms of PMS. Molecular genetic analysis of ESR1 gene polymorphism in 50 women with PMS (25 women of them had edematous form of disease, 25 - neuropsychical, 25 - mild, 25 - severe form) was carried out. 25 women without diagnosis of PMS were examined as controls. The study A-351G polymorphism estrogen gene ESR1 showed no statistically significant differences in the frequency of distribution of genotypes and alleles between women with PMS and without this pathology. However, the frequency of GG genotype in women with severe PMS was significantly higher in 8.0 times compared with healthy women (χ2=4.87, p=0.03) and in women with edematous form of PMS - in 7.0 times (χ2=3.72, p=0.05). Thus, a polymorphic variant of A-351G gene ESR1 estrogen can be considered as a marker of PMS. Pathological variant GG genotype was significantly associated with the presence of edematous and severe forms of the disease.

The aim of this study was to investigate the possible influence of ivabradine and co-3 polyunsaturated fatty acids on leukocyte' and its populations counts in patients with ischemic heart failure. 357 patients with ischemic HF and sinus rhythm were observed. Additional usage of co-3 PUFA caused a WBC count decrease by 12.3% -12.5% (p<0.05) and neutrophil count by 8.3% - 9.6% (p<0.05). Thus, ω-3 polyunsaturated fatty acids have influence on elevated WBC (especially granulocytes) counts and showed anti-inflammatory effects. Further studies designed to investigate the influence of these medications on other inflammatory markers in heart failure are needed to determine new approach in prediction and prevention of heart failure.

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