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Pakharenko L.V.,Ivano Frankivsk National Medical University
New Armenian Medical Journal | Year: 2015

One of the most common neuroendocrine syndromes in gynecology is premenstrual syndrome. The article is devoted to the study of progesterone level and frequency of polymorphic variants T1T2 of progesterone receptor gene PROGINS, as well as their possible relationship in patients with various forms of PMS. The study involved 50 women of reproductive age with PMS diagnosis, including 25 women with edematous form of the disease, 25 - with neuropsychic form. Of the same 50 patients twenty five women had mild form of the disease, and 25 women had severe form. Control group consisted of 25 women without PMS diagnosis. Progesterone level was determined in blood serum twice in the first and second phase of a menstrual cycle. Polymorphism of T1T2 of PROGINS progesterone receptor gene was studied using polymerase chain reaction. We found hypoprogesteronemia of the luteal phase of a menstrual cycle in women with PMS, especially pronounced in persons with edematous and severe forms of the disease (p<0.05). Polymorphic variant T1T2 of PROGINS gene can be considered as a marker of PMS development. T1T1 genotype was significantly associated with development of edematous form (?2=4.50, ?=0.03) and its presence may indicate a tendency to develop into severe form of PMS (?2=3.21, ?=0.07). T2 allele can be considered as a protective mechanism for the appearance of PMS, particularly its edematous and severe forms, in which the presence of T2 allele reduces the risk of PMS, respectively, by 3.0 (?2=4.50, p=0.03, OR=0.20, 95% CI 0.05-0.78, p=0.02) and by 1.71 times (?2=3.21, p=0.07, OR=0.27, 95% CI 0.08-0.95, p=0.04) compared with the control group. Reduced blood level of progesterone in women with PMS correlated with the presence of T1T1 genotype of PROGINS gene. PMS patients with T1T2 genotype exhibited significantly low levels of this hormone in the second phase of menstrual cycle (p<0.05) compared to women with the same genotype in the control group. Furthermore, women with this neuroendocrine syndrome and T1T2 genotype showed no statistically significant difference in progesterone level in these groups compared with healthy women with the same genotype (p>0.05). © 2015, Yerevan State Medical University. All rights reseved. Source

Pavlyuk V.,Jan Dlugosz University in Czestochowa | Pavlyuk V.,Ivan Franko National University of Lviv | Stetskiv A.,Ivano Frankivsk National Medical University | Rozdzynska-Kielbik B.,Jan Dlugosz University in Czestochowa
Intermetallics | Year: 2013

The isothermal section of the phase diagram of Li-La-Ge ternary system at 400°C in the 30-100 at.% Ge concentration range was built and formation of seven ternary compounds were observed. For all ternary compounds: τ1-LiLa2Ge6-x (Cmmm space group, LiPr 2Ge6 structure type), τ2-LiLaGe2 (Pnma, LiCaSi2), τ3-Li2La 2Ge3 (Cmcm, Li2Ce2Ge3), τ4-Li8La7Ge10 (Cmmm, own structure type), τ5-Li12La11Ge16 (Immm, own structure type), τ6-Li4La 3Ge4 (Immm, Gd3Cu4Ge4) and τ7-LiLa4Ge4 (Pnma, LiTm 4Ge4) the crystal structures are known. The Li 2La2Ge3, Li12La11Ge 16, Li8La7Ge10 and Li 4La3Ge4 ternary phases form the homologous series which is based on the simple Al2MgCu and AlB2 structure types. The crystal structures of LixLa5Ge 3 phase, which formed by incorporation of lithium atoms into octahedral voids of La5Ge3 binary phase, was studied more precisely by X-ray powder diffraction and electrochemical investigation. According to electronic structure calculations using the tight-binding linear muffin-tin orbital-atomic spheres approximations (TB-LMTO-ASA) method the strong covalent Ge-Ge interactions were established. © 2013 Elsevier Ltd. All rights reserved. Source

Perederko L.P.,Ivano Frankivsk National Medical University
Zoology and Ecology | Year: 2014

The paper presents the results of investigation of the amount of biogenic nutrients in the blood of the African Ostrich (Struthio camelus) fed low mineral content diets in the temperate continental climate in the western region of Ukraine. Blood samples were taken from the wing vein. The serum of males of about two years of age was used for analysis. In the study area, Struthio camelus were bred in an intensive manner, where the birds were in limited areas with free access outside. The amount of minerals in the feed strongly affected the level of biogenic nutrients in blood. The study of haematological parameters of trace minerals in the blood of African Ostriches is of great diagnostic value in determining the internal changes and pathological diagnosis of defects in the organism. © 2014 Nature Research Centre. Source

Pakharenko L.,Ivano Frankivsk National Medical University
Georgian medical news | Year: 2014

To identify risks of development of any disease is a priority of modern medicine. The aim of this study was to investigate the frequency of polymorphic variants of A-351G gene estrogen receptor ESR1 in patients with various forms of PMS. Molecular genetic analysis of ESR1 gene polymorphism in 50 women with PMS (25 women of them had edematous form of disease, 25 - neuropsychical, 25 - mild, 25 - severe form) was carried out. 25 women without diagnosis of PMS were examined as controls. The study A-351G polymorphism estrogen gene ESR1 showed no statistically significant differences in the frequency of distribution of genotypes and alleles between women with PMS and without this pathology. However, the frequency of GG genotype in women with severe PMS was significantly higher in 8.0 times compared with healthy women (χ2=4.87, p=0.03) and in women with edematous form of PMS - in 7.0 times (χ2=3.72, p=0.05). Thus, a polymorphic variant of A-351G gene ESR1 estrogen can be considered as a marker of PMS. Pathological variant GG genotype was significantly associated with the presence of edematous and severe forms of the disease. Source

Kozovyi R.V.,Ivano Frankivsk National Medical University | Podolska S.V.,Shupyk National Medical Academy of Postgraduate Education | Gorovenko N.G.,Shupyk National Medical Academy of Postgraduate Education
Advances in Gerontology | Year: 2014

Polymorphism of genes of Phase II of xenobiotic biotransformation was studied in 166 long-lived people and in 169 control subjects who live in the Ivano-Frankivsk region. The frequency of the functionally inactive allele of the GSTT1 gene among the long-lived people was 24.70% and in the control group it was 20.12%. The frequency of the functionally inactive allele of GSTM1 in the long-lived people was 46.99 and in the control group it was 54.44%. The area of residence of the subjects was divided into zones with regard to environmental factors: environmentally comfortable, under moderate environmental load, and environmentally uncomfortable. Analysis of the combinations of alleles of glutathione-S-transferase genes revealed an elevated frequency of the GSTM1+/GSTT1+ allele combination in the long-lived people of the uncomfortable zone in comparison to the control group of the same zone: 54.55 and 35.09%, respectively (χ2 = 4.29; OR = 2.22 (1.04-4.75)). The GSTM1-/GSTT1+ combination was significantly more frequent in the control group than in the long-lived people: 21.82 and 43.86%, respectively (χ2 = 6.15; OR = 0.36 (0.16-0.82)). Significant differences were detected between the frequencies of combinations GSTM1+/GSTT1+, GSTM1+/GSTT1-, GSTM1-/GSTT1+, and GSTM1-/GSTT1- in long-lived people of the environmentally comfortable and uncomfortable zones: χ2 = 6.44, OR = 0.36 (0.16-0.80); χ2 = 4.86, OR = 4.89 (1.28-18.72); χ2 = 5.89, OR = 2.82 (1.20-6.58); χ2 = 3.90; OR = 0.19 (0.16-6.58), respectively. © 2014 Pleiades Publishing, Ltd. Source

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