Indianapolis, IN, United States
Indianapolis, IN, United States
Time filter
Source Type

Bottino M.C.,Indiana University | Batarseh G.,IUSD | Palasuk J.,Indiana University | Alkatheeri M.S.,Indiana University | And 2 more authors.
Dental Materials | Year: 2013

Objective The aim of this study was to investigate the effect of aluminosilicate clay nanotubes (Halloysite, HNT) incorporated into the adhesive resin of a commercially available three-step etch and rinse bonding system (Adper Scotchbond Multi-Purpose/SBMP) on dentin bond strength, as well as the effect on several key physicochemical properties of the modified adhesive. Methods Experimental adhesives were prepared by adding five distinct HNT amounts (5-30 wt.%) into the adhesive resin (w/v) of the SBMP dentin bonding system. Bond strength to human dentin, microhardness, and degree of conversion (DC) of the modified adhesives were assessed. Results From the shear bond strength data, it was determined that HNT incorporation at a concentration of 30 wt.% resulted in the highest bond strength to dentin that was statistically significant (p = 0.025) when compared to the control. Even though a significant increase in microhardness (p < 0.001) was seen for the 30 wt.% HNT-incorporated group, a significantly lower DC (p < 0.001) was recorded when compared to the control. Significance It was concluded that HNT can be incorporated up to 20 wt.% without jeopardizing important physicochemical properties of the adhesive. The modification of the SBMP dentin bonding agent with 20 wt.% HNT appears to hold great potential toward contributing to a durable dentin bond; not only from the possibility of strengthening the bond interface, but also due to HNT intrinsic capability of encapsulating therapeutic agents such as matrix metalloproteinase (MMP) inhibitors. © 2013 Academy of Dental Materials.

Munchow E.A.,Indiana University | Munchow E.A.,Federal University of Pelotas | Albuquerque M.T.P.,Indiana University | Albuquerque M.T.P.,São Paulo State University | And 5 more authors.
Dental Materials | Year: 2015

Abstract Objectives This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods Poly(ε-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibers were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (α = 5%). Results ZnO nanoparticles were successfully incorporated into the overall submicron fibers, which showed fairly good morphology and microstructure. Upon ZnO nanoparticles' incorporation, the PCL and PCL/GEL fibers became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane. © 2015 Academy of Dental Materials.

Sabrah A.H.A.,Indiana University | Cook N.B.,Indiana University | Luangruangrong P.,Indiana University | Hara A.T.,IUSD | Bottino M.C.,Indiana University
Dental Materials | Year: 2013

Objective To investigate the effects of polishing techniques on the surface roughness of Y-TZP ceramic and on the wear behavior of synthetic hydroxyapatite (HA). Methods Thirty-two full-contour Y-TZP (Diazir®) sliders (φ = 2 mm × 1.5 mm in height) were manufactured using CAD/CAM, embedded in acrylic resin using brass holders, and randomly allocated into four groups (n = 8): according to the finishing/polishing procedure: G1-as-machined, G2-glazed, G3-diamond bur finishing and G4 - G3 + OptraFine® polishing kit. Thirty-two sintered HA disks (φ = 13 mm × 2.9 mm in height) were similarly mounted in brass holders. Y-TZP sliders baseline surface roughness values (Ra and Rq, in μm) were recorded using a non-contact profilometer (Proscan 2000). A two-body pin-on-disc wear test was performed. HA height (μm) and volume (mm3) losses were measured. Y-TZP height loss was measured using a digital micrometer. One-way ANOVA was used to determine the effect of the polishing techniques on the surface roughness. Comparisons between groups for differences in antagonist height loss/volume, and slider height loss were performed using one-way ANOVA. Statistical significance was set at α = 0.05. Results Roughness measurements showed significant differences (p = 0.0001) among the surface treatments with G1 (Ra = 0.84, Rq = 1.13 μm) and G3 (Ra = 0.89, Rq = 1.2 μm) being the roughest, and G2 (Ra = 0.42, Rq = 0.63 μm) the smoothest (p = 0.0001). Y-TZP slider height loss was highest for the glazed group (35.39 μm), and was lowest for the polished group (6.61 μm) (p = 0.0001). Antagonist volume and height losses for groups (G1-G3) were similar, while the polished group (1.3 mm3, 14.7 μm) showed significant lower values (p = 0.0001). Significance Although glazed zirconia provides an initially smooth surface, significantly increased antagonist wear was observed compared to the polished Y-TZP zirconia surface. © 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Bottino M.C.,Indiana University | Yassen G.H.,Indiana University | Platt J.A.,Indiana University | Labban N.,IUSD | And 4 more authors.
Journal of Tissue Engineering and Regenerative Medicine | Year: 2015

An electrospun nanocomposite fibrous material holds promise as a scaffold, as well as a drug-delivery device to aid in root maturogenesis and the regeneration of the pulp-dentine complex. A novel three-dimensional (3D) nanocomposite scaffold composed of polydioxanone (PDS II®) and halloysite nanotubes (HNTs) was designed and fabricated by electrospinning. Morphology, structure, mechanical properties and cell compatibility studies were carried out to evaluate the effects of HNTs incorporation (0.5-10wt% relative to PDS w/w). Overall, a 3D porous network was seen in the different fabricated electrospun scaffolds, regardless of the HNT content. The incorporation of HNTs at 10wt% led to a significant (p<0.0001) fibre diameter increase and a reduction in scaffold strength. Moreover, PDS-HNTs scaffolds supported the attachment and proliferation of human-derived pulp fibroblast cells. Quantitative proliferation assay performed with human dental pulp-derived cells as a function of nanotubes concentration indicated that the HNTs exhibit a high level of biocompatibility, rendering them good candidates for the potential encapsulation of distinct bioactive molecules. Collectively, the reported data support the conclusion that PDS-HNTs nanocomposite fibrous structures hold potential in the development of a bioactive scaffold for regenerative endodontics. © 2015 John Wiley & Sons, Ltd.

PubMed | Indiana University and IUSD
Type: | Journal: Odontology | Year: 2016

The purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), and Aggregatibacter actinomycetemcomitans (Aa)]. Tetracycline hydrochloride (TCH) was added to a poly(DL-lactide) [PLA], poly(-caprolactone) [PCL], and gelatin [GEL] polymer blend solution at distinct concentrations to obtain the following fibers: PLA:PCL/GEL (TCH-free, control), PLA:PCL/GEL+5% TCH, PLA:PCL/GEL+10% TCH, and PLA:PCL/GEL+25% TCH. The inhibitory effect of TCH-containing fibers on biofilm formation was assessed by colony-forming units (CFU/mL). Qualitative analysis of biofilm inhibition was done via scanning electron microscopy (SEM). Statistical significance was reported at p<0.05. Complete inhibition of biofilm formation on the fibers was observed in groups containing TCH at 10 and 25wt%. Fibers containing TCH at 5wt% demonstrated complete inhibition of Aa biofilm. Even though a marked reduction in CFU/mL was observed with an increase in TCH concentration, Pi proved to be the most resilient microorganism. SEM images revealed the absence of or a notable decrease in bacterial biofilm on the TCH-containing nanofibers. Collectively, our data suggest that tetracycline-containing fibers hold great potential as an antibacterial dental implant coating.

Loading IUSD collaborators
Loading IUSD collaborators