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Onori R.,Italian Institute for Health ISS | Lopardo R.,Metapontum Agrobios | De Giacomo M.,Italian Institute for Health ISS | De Santis B.,Italian Institute for Health ISS | And 3 more authors.
Food Control | Year: 2013

A study on the fate of Genetically Modified (GM) Roundup Ready soybean (RRS) was undertaken on the following products: flour, protein flour, lecithin, crude and refined oil, broken grain, hull and expander of an industrial soybean manufacturing plant, with the aim to evaluate the possible effects of processing on the reliability of control plans. A sampling control plan was applied to all the products of the industrial manufactory plant. The best sampling point was identified based on the lowest impact of the analytical and sampling uncertainty.The best "fit for purpose" sampling point for the accurate evaluation of the Genetically Modified Organism (GMO) concentration measurement was identified in the processed products, e.g. flour and protein flour, thanks to the homogeneity on RRS in the batch and the better yield and quality of the extracted DNA.This study presents a practical approach to assess the two main factors that affect the reliability of the control plans: analytical and sampling uncertainty. The work was undertaken on GM soybean derived products, nevertheless the conclusions we reached could be also applied to verify compliance with GMO labelling threshold. © 2013 Elsevier Ltd. Source

Brera C.,Italian Institute for Health ISS | De Santis B.,Italian Institute for Health ISS | Prantera E.,Italian Institute for Health ISS | Debegnach F.,Italian Institute for Health ISS | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

Use of proper sampling methods throughout the agri-food chain is crucial when it comes to effectively detecting contaminants in foods and feeds. The objective of the study was to estimate the performance of sampling plan designs to determine aflatoxin B1 (AFB1) contamination in corn fields. A total of 840 ears were selected from a corn field suspected of being contaminated with aflatoxin. The mean and variance among the aflatoxin values for each ear were 10.6 μg/kg and 2233.3, respectively. The variability and confidence intervals associated with sample means of a given size could be predicted using an equation associated with the normal distribution. Sample sizes of 248 and 674 ears would be required to estimate the true field concentration of 10.6 μg/kg within ±50 and ±30%, respectively. Using the distribution information from the study, operating characteristic curves were developed to show the performance of various sampling plan designs. © 2010 American Chemical Society. Source

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