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Sant'Ambrogio di Torino, Italy

Dalmasso S.,University of Turin | Rossi L.,University of Turin | Molinar Min A.R.,University of Turin | Gennero S.,Istituto Zooprofilattico Sperimentale Piemonte | And 2 more authors.
Small Ruminant Research | Year: 2012

Reliable figures of local distribution and prevalence of cystic echinococcosis (CE) in intermediate hosts are a fundamental prerequisite for implementation of control strategies against cystic echinococcosis (CE), caused by Echinococcus granulosus. With the aim to assess the " true" prevalence of CE in a mountain area of Piedmont region (North-Western Italy), two methods alternative to use of official abattoir data were compared: (i) the necroscopic examination of 117 sheep and goats, killed during wolf attacks while on summer pastures, for presence of hydatid cysts; (ii) the serological examination with an enzyme-linked immuno-electro transfer blot assay (EITB) of 1217 sheep from 9 transhumant flocks for presence of anti-Echinococcus antibodies. EITB was first performed on pooled samples, then each serum sample from positive pools was individually tested. Prevalences were 15.4 and 10.8% with method (i) and method (ii), respectively, and they resulted not statistically different (P>. 0.05). Seroreactors (from 4.4 to 46%) were found in 7 of 9 tested flocks. Adult Echinococcus tapeworms were found in 2 of 4 necropsied dogs from 3 flocks. On the basis of the results, CE prevalence in small ruminants from Piedmont seems to be higher than that reported by official abattoir statistics in the last decade. © 2012 Elsevier B.V..

Decastelli L.,Istituto Zooprofilattico Sperimentale Piemonte | Gallina S.,Istituto Zooprofilattico Sperimentale Piemonte | Manila Bianchi D.,Istituto Zooprofilattico Sperimentale Piemonte | Fragassi S.,Istituto Zooprofilattico Sperimentale Piemonte | Restani P.,University of Milan
Food Additives and Contaminants: Part B Surveillance | Year: 2012

Several EC Directives have been promulgated to protect allergic individuals but no rule has been established with regard to allergen cross-contamination caused by shared transport vehicles or common processing equipment. The aim of this research was to quantify, by enzyme-linked immunosorbent assay (ELISA) or real-time polymerase chain reaction, the presence in meat- or fish-based foods of four allergens (milk, egg, crustaceans and molluscs) that was not indicated either in the list of ingredients or in the label alert. In the time frame of 2007-2009, a total of 723 samples were subjected to 1983 analyses. The percentage of samples scoring positive ranged between 1.8% and 6.8% over the 3 years, and the concentrations of undeclared allergens found were 0.3-13.3 mg kg -1 for milk (β-lactoglobulin) and 0.21-12 mg kg -1 for egg white proteins. On this basis, the possibility of cross-contamination serious enough to raise public health concern cannot be dismissed. © 2012 Copyright Taylor and Francis Group, LLC.

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