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Bonetta S.,University of Piemonte Orientale | Ferretti E.,University of Piemonte Orientale | Fezia G.,Istituto Zooprofilattico Sperimentale del Piemonte | Carraro E.,University of Piemonte Orientale
Letters in Applied Microbiology

Aims: This study was performed to investigate the microbiological contamination of digestate product (DP) obtained from the anaerobic co-digestion of bovine manure and agricultural by-products. Methods and results: Microbiological analyses were performed on bovine manure, fresh DP, liquid and solid fractions and stored liquid fraction of DP. A statistically significant reduction of faecal bacterial indicator was found after anaerobic digestion except for enterococci. After liquid/solid DP separation, bacteria tend to be concentrated in the solid fraction. Storage does not seem to influence the indicator parameters, except for enterococci. Escherichia coli O157:H7 and Yersinia were not found in any samples analysed. Salmonella was rarely detected in DP samples and its derivates, while Listeria monocytogenes was encountered in many samples. Conclusions: The results obtained indicate that the hygienic quality of DP is for almost all microbiological parameters better than that of the bovine manure (range of reduction 1.6-3.1 log 10) and suggest the need to identify specific pathogen indicators related to the hygienic characteristics of DPs. Significance and Impact of the Study: This study highlights that the anaerobic co-digestion of bovine manure and agricultural by-products in a field-scale biogas plant does not increase human health risk with respect to the use of animal manure for agricultural fertilization. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology. Source

Ottaviani D.,Laboratorio Nazionale Of Riferimento Contaminazioni Batteriologiche Dei Molluschi Bivalvi | Leoni F.,Laboratorio Nazionale Of Riferimento Contaminazioni Batteriologiche Dei Molluschi Bivalvi | Serra R.,Azienda Ospedaliera San Giovanni Battista di Turin | Serracca L.,Istituto Zooprofilattico Sperimentale del Piemonte | And 6 more authors.
Journal of Clinical Microbiology

We investigated the virulence properties of four Vibrio parahaemolyticus strains causing acute gastroenteritis following consumption of indigenous mussels in Italy. The isolated strains were cytotoxic and adhesive but, surprisingly, lacked tdh, trh, and type three secretion system 2 (T3SS2) genes. We emphasize that nontoxigenic V. parahaemolyticus can induce acute gastroenteritis, highlighting the need for more investigation of the pathogenicity of this microorganism. Copyright © 2012, American Society for Microbiology. All Rights Reserved. Source

Mazzariol S.,University of Padua | Peletto S.,Istituto Zooprofilattico Sperimentale del Piemonte | Mondin A.,University of Padua | Centelleghe C.,University of Padua | And 4 more authors.
Journal of Clinical Microbiology

During the second morbillivirus epidemic (2007 to 2011) in cetaceans along the Italian coastline, dolphin morbillivirus (DMV) was detected by molecular analyses in a captive harbor seal (Phoca vitulina), with pathological findings consistent with morbillivirus infection. This report confirms interspecies DMV transmission from cetaceans to pinnipeds. Copyright © 2013, American Society for Microbiology. Source

Capra P.,Istituto Zooprofilattico Sperimentale del Piemonte | Ciccotelli V.,Istituto Zooprofilattico Sperimentale del Piemonte | Vincenti M.,Centro Regionale Antidoping E Of Tossicologia Alessandro Bertinaria | Vincenti M.,University of Turin
Analytica Chimica Acta

An analytical, pharmacokinetic and histopathologic investigation was conducted by two experimental trials on beef cattle in order to determine fate and effects of dexamethasone and prednisolone, administered to distinct cattle groups at low dosage for long periods of time. In trial 1, eighteen Charolaise beef cattle, male, 17-22-months-old, were divided in three groups: to group A (n=6) dexamethasone-21-sodium-phosphate 0.7mgday-1 per os for 40 days was administered; group B (n=6) was orally treated with prednisolone 15mgday-1 for 30 days, while group C (n=6) served as negative control. Urine was collected at days 0, 7, 15, 25 and 47 from groups A and C, and at days 0, 8, 18 and 42 from group B. In trial 2, sixteen Friesian cattle, male, 10-17-months-old, were randomly divided into two groups: group D (n=8) was administered prednisolone 30mgday-1 per os for 35 days, while group K (n=8) served as control. In both trials, the animals were slaughtered after a 6-days drug withdrawal and thymus and livers were collected and properly stored until the analysis was performed. Quantitative determinations of dexamethasone, prednisolone and its main metabolite, prednisone, in urine and liver samples were conducted by HPLC-MS/MS, after the analytical procedure was optimized and fully validated. The method validation included the assessment of specificity, linearity, precision, trueness, robustness, CCα and CCβ values. By a morphological point of view, severe atrophy of thymus parenchyma was observed in group A, together with a significant (P< 0.005) reduction of the mean thymus weight (217 ± 94. g), while group B (646 ± 215. g) presented normal thymus features and weights (group C, 415 ± 116. g). Accordingly, no differences were found in trial 2 for groups D (727 ± 275. g) and K (642 ± 173. g).Average dexamethasone concentrations in group A urine samples ranged from 1.4 to 3.0μgL-1 during the treatment, while no residue was detected in the urine samples collected 6-7 days after the end of the treatment. Low amounts of dexamethasone (<1μgL-1) were detected in liver samples of group A. All average prednisolone concentrations in group B urine samples (sum of conjugate and free form) turned out to be below 1.0μgL-1 during the treatment, despite the much higher concentration administered (15-30mgday-1) with respect to dexamethasone in group A (0.7mgday-1). No prednisolone residues were found in the urine and liver samples taken at the slaughterhouse. The absence of any prednisolone residue in the urine samples of control group animals supports the theory that the origin of this molecule is fundamentally exogenous, at least for this cattle category maintained under unstressing conditions. Remarkable findings are represented by the absence of thymus atrophy in the prednisolone treated animals and the extremely low residue concentrations found in urine during the treatment. Both findings reveal that the detection of illegal growth-promoting treatments with this drug is difficult. © 2010 Elsevier B.V. Source

Leporati M.,Centro Regionale Antidoping E Of Tossicologia Alessandro Bertinaria | Capra P.,Istituto Zooprofilattico Sperimentale del Piemonte | Brizio P.,Istituto Zooprofilattico Sperimentale del Piemonte | Ciccotelli V.,Istituto Zooprofilattico Sperimentale del Piemonte | And 3 more authors.
Journal of Separation Science

A selective and sensitive method for screening 31 analytes (nine corticosteroids, eight β-agonists, seven anabolic steroids, six promazines and zeranol) in bovine urine was validated according to 2002/657/EC guidelines. Upon optimization of sample treatment conditions, the extraction was performed by diethylether at pH 9, after deconjugation. Extraction yields (R%) proved higher than 70% for 19 analytes, 50 Source

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