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Puzelli S.,Istituto Superiore di Sanita | Rossini G.,University of Bologna | Facchini M.,Istituto Superiore di Sanita | Vaccari G.,Istituto Superiore di Sanita | And 14 more authors.
Emerging Infectious Diseases | Year: 2014

During an influenza A(H7N7) virus outbreak among poultry in Italy during August–September 2013, infection with a highly pathogenic A(H7N7) avian influenza virus was diagnosed for 3 poultry workers with conjunctivitis. Genetic analyses revealed that the viruses from the humans were closely related to those from chickens on affected farms. © 2014, Emerging Infectious Diseases. All rights reserved. Source

Labella A.,University of Malaga | Gennari M.,University of Verona | Ghidini V.,University of Verona | Trento I.,University of Verona | And 3 more authors.
Marine Pollution Bulletin | Year: 2013

Marine bacteria exposed to antibiotics in fish farms can acquire antimicrobial resistance by mobile genetic elements and horizontal gene transfer. A total of 872 autochthonous marine bacterial strains was isolated from samples collected from four different fish farms located at northern and southern Italian Adriatic Sea. Resistance to only tetracycline (17%) and to trimethoprim-sulfadiazine (7%) were the most frequent patterns obtained, while flumequine resistance has recorded in only 0.3% of the strains. Comparing strains isolated from coastal areas and fish farms, a significant higher incidence (4% versus 10%) of multi-resistant strains in aquaculture centers was found. Significant differences in antibiotic resistance incidence were also detected among the four fish farms due probably to different approaches in farm management and the more or less frequent use of antibiotics. Antibiotic-resistant and multi-resistant strains isolated constitute an environmental reservoir directly involved in the seafood chain and might represent a public health concern. © 2013 Elsevier Ltd. Source

Dalipi R.,University of Brescia | Borgese L.,University of Brescia | Zacco A.,University of Brescia | Tsuji K.,Osaka City University | And 4 more authors.
International Journal of Environmental Analytical Chemistry | Year: 2015

This work was performed to highlight the advantages of total reflection X-ray fluorescence spectroscopy (TXRF) for multi-elemental qualitative and quantitative analysis of wine. Indeed the International Organization of Vine and Wine (OIV) selected some potentially toxic elements and proposed limit values for their concentration in wines. Direct TXRF analysis of nine wine samples from Emilia Romagna region of Italy was performed in two different laboratories: Italy and Japan. Wine dehydration was also evaluated as sample conservation mean. Traces of Fe, Cu, Zn and Pb are present in all the analysed samples, with concentrations lower than the limits established by the OIV. The target hazard quotients (THQs) were also calculated for seven elements (Cl, Mn, Fe, Ni, Cu, Zn and Sr) to determine their potential detrimental effects. The results show that TXRF is a fast, simple and accurate analytical technique for trace element analysis of wine. Moreover, dehydration is an effective way to store wine samples for further elemental analysis. © 2015 Taylor & Francis. Source

Di Cesare A.,University of Teramo | Veronesi F.,University of Perugia | Grillotti E.,University of Teramo | Grillotti E.,Ambulatorio Veterinario Centro Italia | And 8 more authors.
Parasitology Research | Year: 2015

The occurrence of common respiratory parasites of domestic cats (the metastrongyloid “cat lungworm” Aelurostrongylus abstrusus and the trichuroid Capillaria aerophila) and of neglected respiratory nematodes of felids (Troglostrongylus brevior, Angiostrongylus chabaudi and Oslerus rostratus) was here evaluated in two and three geographical sites of Northern and Central Italy, respectively. In 2014–2015, individual fecal samples of 868 domestic cats were examined microscopically and genetically, and epidemiological data related to parasitic infections were evaluated as possible risk factors by binary logistic regression models. The most common parasite was A. abstrusus in both mono- and poli-specific infections, followed by T. brevior and C. aerophila, while cats scored negative for other parasites. Cats positive for A. abstrusus (1.9–17 % infection rate) and C. aerophila (0.9–4.8 % infection rate) were found in all examined sites, while cats scored positive for T. brevior (1–14.3 % infection rate) in four sites. Also, T. brevior was here found for the first time in a domestic cat from a mountainous area of Northern Italy. The occurrence of lungworms was statistically related to the presence of respiratory signs and more significant in cats with mixed infection by other lungworms and/or intestinal parasites. Cats living in site C of Central Italy resulted statistically more at risk of infection for lungworms than cats living in the other study sites, while animals ageing less than 1 year were at more risk for troglostrongylosis. Finally, the presence of lungworms was more significant in cats with mixed infection by other lungworms and/or intestinal parasites. These results are discussed under epidemiological and clinical points of views. © 2015, Springer-Verlag Berlin Heidelberg. Source

De Liguoro M.,University of Padua | Bona M.D.,University of Padua | Gallina G.,University of Padua | Capolongo F.,University of Padua | And 3 more authors.
Environmental Science and Pollution Research | Year: 2014

In this study, 50 livestock watering sources (ground water) and 50 field irrigation sources (surface water) from various industrialised areas of the Veneto region were monitored for chemical contaminants. From each site, four water samples (one in each season) were collected during the period from summer 2009 through to spring 2010. Surface water samples and ground water samples were first screened for toxicity using the growth inhibition test on Pseudokirchneriella subcapitata and the immobilisation test on Daphnia magna, respectively. Then, based on the results of these toxicity tests, 28 ground water samples and 26 surface water samples were submitted to chemical analysis for various contaminants (insecticides/acaricides, fungicides, herbicides, metals and anions) by means of UPLC-MSn HPLC-MSn, AAS and IEC. With the exception of one surface water sample where the total pesticides concentration was greater than 4 μg L-1, positive samples (51.9 %) showed only traces (nanograms per liter) of pesticides. Metals were generally under the detection limit. High concentrations of chlorines (up to 692 mg L-1) were found in some ground water samples while some surface water samples showed an excess of nitrites (up to 336 mg L-1). Detected levels of contamination were generally too low to justify the toxicity recorded in bioassays, especially in the case of surface water samples, and analytical results painted quite a reassuring picture, while tests on P. subcapitata showed a strong growth inhibition activity. It was concluded that, from an ecotoxicological point of view, surface waters used for field irrigation in the Veneto region cannot be considered safe. © 2013 Springer-Verlag Berlin Heidelberg. Source

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