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Tiberi F.,Catholic University of the Sacred Heart | Tropea A.,Cattedra di Fisiopatologia della Riproduzione Umana | Apa R.,Cattedra di Fisiopatologia della Riproduzione Umana | Romani F.,Cattedra di Fisiopatologia della Riproduzione Umana | And 2 more authors.
Fertility and Sterility | Year: 2010

Objective: To examine prokineticin 1 (PROK1) mRNA expression in eutopic endometrial glands obtained from patients with or without endometriosis, to investigate the presence of additional endometrial abnormalities in women with endometriosis. Design: Prospective laboratory study. Setting: University hospital. Patients: Twelve control women and 12 patients affected by endometriosis in the secretory phase of the menstrual cycle. Intervention(s): Endometrial specimens were obtained from women affected (cases) or not (control group) by endometriosis. Endometrial glands were freshly isolated from endometrial biopsies. Main Outcome Measure(s): PROK1 mRNA expression levels by real-time polymerase chain reaction analysis. Results: PROK1 mRNA was detectable in 4 of 12 (33%) samples obtained from women affected by endometriosis, whereas 10 of 12 (83%) samples obtained from normal women were positive for PROK1 detection by realtime polymerase chain reaction. Moreover, detectable PROK1 mRNA levels were 10 times lower in samples obtained from women with endometriosis than in samples obtained from control women. Conclusion(s): PROK1 is a newly discovered angiogenic factor implicated in the vascular function of peri-implantation endometrium and early pregnancy. An altered expression of PROK1 could be one of the several biochemical abnormalities characterizing eutopic endometrium in endometriosis. © 2010 by American Society for Reproductive Medicine. Source


Romani F.,Catholic University of the Sacred Heart | Lanzone A.,Istituto di Ricerca Associazione Maria ONLUS | Tropea A.,Catholic University of the Sacred Heart | Familiari A.,Catholic University of the Sacred Heart | And 5 more authors.
Fertility and Sterility | Year: 2012

Objective: To evaluate whether unacylated ghrelin and obestatin were able to influence human luteal cell function. The effect of these two ghrelin-related peptides on progesterone (P4), prostaglandin (PG) F 2α, PGE 2, and vascular endothelial growth factor (VEGF) release and on VEGF expression in isolated human steroidogenic cells has been investigated. Design: Prospective laboratory study. Setting: University hospital. Patient(s): Corpora lutea were obtained from 23 normally menstruating patients in the midluteal phase of the menstrual cycle. Intervention(s): Human luteal cells were isolated from corpora lutea, and primary cultures were established. Main Outcome Measure(s): P4 and PGs release was assayed by enzyme immunoassay, VEGF secretion by ELISA, and VEGF mRNA expression by real-time polymerase chain reaction. Result(s): P4 and VEGF release were significantly reduced by both unacylated ghrelin and obestatin. Moreover, the highest concentration of obestatin was able to reduce the release of PGE 2 and PGF 2α. VEGF mRNA expression was not affected by the incubation with any of these ghrelin-related peptides. As expected, CoCl 2 was able to induce VEGF release and mRNA expression in luteal cells. Conclusion(s): Our results suggest that, similar to ghrelin, both unacylated ghrelin and obestatin might play a role in regulating the luteal cell function that affects both luteal steroidogenesis and luteotrophic/luteolytic imbalance. These results further underline the pivotal correlation between the ghrelin system and reproduction. © 2012 by American Society for Reproductive Medicine. Source


Romani F.,Catholic University of the Sacred Heart | Lanzone A.,Istituto di Ricerca Associazione Maria ONLUS | Tropea A.,Catholic University of the Sacred Heart | Tiberi F.,Istituto Scientifico Internazionale Paolo | And 2 more authors.
Placenta | Year: 2011

Objective: To examine nicotine (N) and cotinine (C) effects on trophoblast cells (TCs) and human umbilical vein endothelial cells (HUVEC) secretion of soluble fms-like tyrosine kinase (sFlt-1), soluble endoglin (sENG), placental growth factor (PlGF), transforming growth factor-beta (TGF-beta) and vascular endothelial growth factor (VEGF). Study design: Human placentas and umbilical cords were collected from uncomplicated pregnancies at term from a total of 24 non-smoking women with a history of normal blood pressure. TCs and HUVEC were cultured for 24 h with C or N (from 10 -12 to 10 -7 M). Main outcome measures: sFlt-1, sENG, PlGF, TGF-beta and VEGF release and messenger RNA (mRNA) expression were evaluated by ELISA and real-time polymerase chain reaction (PCR), respectively. Results: N and C reduced sFlt-1, sENG and PlGF release by TCs and TGF-beta release by HUVEC. Conversely, N and C increased PlGF secretion, while N alone increased sFlt-1 release by HUVEC. N and C were able to modulate VEGF mRNA expression in HUVEC. Conclusions: Our results suggest that N and C affect the balance of some important vasoactive factors released by TCs and HUVEC. This might be one of the possible mechanism through which smoke reduces the risk of hypertensive disorders during pregnancy as well as contributes to the well known detrimental effects of smoking on fetal development. © 2010 Elsevier Ltd. All rights reserved. Source


Romani F.,Catholic University of the Sacred Heart | Guido M.,Catholic University of the Sacred Heart | Morciano A.,Catholic University of the Sacred Heart | Martinez D.,Catholic University of the Sacred Heart | And 3 more authors.
Archives of Gynecology and Obstetrics | Year: 2013

Purpose: To evaluate the successful rate and patient acceptance of different-sized hysteroscope in office hysteroscopy. Methods: We retrospectively evaluated 900 office hysteroscopy performed in ambulatory setting using three different hysteroscopes: 5 mm Hamou II (n = 300), 5 mm Bettocchi (n = 300) and 4 mm Bettocchi (n = 300). Endpoints of our study were the successful rate of hysteroscopy, the eventual side effects/complication and the pain intensity experience from the patients using visual analog scale (VAS). Results: Use of 4 mm Bettocchi leads to a higher rate of successfully performed hysteroscopy (99 %, n = 297) and statistically significant when compared to the 5 mm Hamou (95 %, n = 285) and to the 5 mm Bettocchi (96 %, n = 288) (4 mm Bettocchi vs. 5 mm Bettocchi p < 0.05; 4 mm Bettocchi vs. 5 mm Hamou II p < 0,001; 5 mm Bettocchi vs. 5 mm Hamou II ns). Moreover, the VAS score was higher using 5 mm Hamou II (5.72 ± 1.99) and statistically significant when compared to the 4 mm Bettocchi (3.06 ± 2.14) and to the 5 mm Bettocchi (4.27 ± 1.88) (A vs. B p < 0.05; A vs. C p < 0.001; B vs. C p < 0.001). Conclusions: Our result suggests that the hysteroscope size plays a pivotal role in the acceptance and for the success of office hysteroscopy. © 2013 Springer-Verlag Berlin Heidelberg. Source


Romani F.,Catholic University of the Sacred Heart | Tropea A.,Catholic University of the Sacred Heart | Scarinci E.,Catholic University of the Sacred Heart | Russo C.D.,Catholic University of the Sacred Heart | And 4 more authors.
Journal of Environmental Science and Health - Part C Environmental Carcinogenesis and Ecotoxicology Reviews | Year: 2013

Endocrine disruptors are well known to impair fertility. The aim of the present study was to investigate the effects of bisphenol A (BPA) and nonylphenol (p-NP) on human luteal function in vitro. In particular, in luteal cells isolated from 21 human corpora lutea progesterone, prostaglandin (PG) F2α, PGE2 and vascular endothelial growth factor (VEGF) release, as well as VEGF expression were evaluated. BPA and p-NP negatively affected both luteal steroidogenesis and luteotrophic/ luteolytic factors balance, without influencing VEGF mRNA expression. Actually, BPA and p-NP impaired human luteal cells function in vitro, underlining the already suggested correlation between phenols and reproductive failure. © 2013 Copyright Taylor and Francis Group, LLC. Source

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