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Caporossi L.,ISPESL National Institute of Occupational Safety and Prevention | Santoro A.,University of Rome La Sapienza | Papaleo B.,ISPESL National Institute of Occupational Safety and Prevention
Biomarkers | Year: 2010

Analytical tests to measure chemicals in saliva can be employed for numerous analytes, endogenous compounds or xenobiotics. The objective was to determine which chemicals can be analysed with this matrix, which analytical methods are applicable, and what application is possible for biomonitoring. We reviewed the literature using three databases, MEDLINE, PubMed and Scopus, collecting articles on different kinds of analysis in saliva. Studies were principally about molecules of clinical interest, xenobiotics, especially drugs of abuse, and chemicals used at workplaces; some substances show no relevant correlation with exposure data while others seems to be of particular interest for systematic use for biomonitoring. Currently, saliva is used far less than other biological fluids but its use for biomonitoring of exposure to chemicals might open up new areas for research and would certainly simplify the collection of biological samples. © 2010 Informa UK, Ltd. Source


Caporossi L.,ISPESL National Institute of Occupational Safety and Prevention | De Rosa M.,ISPESL National Institute of Occupational Safety and Prevention | Papaleo B.,ISPESL National Institute of Occupational Safety and Prevention
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2010

To determine the biomarkers of exposure to xylene, urinary 2-, 3- and 4-methyl-hippuric acids, a new HPLC/DAD analytical method has been developed, which uses β-cyclodextrin as an additive for elution; its complexing abilities are exploited to achieve complete chromatographic separation of the three isomers. The mobile phase was a 3% aqueous solution of β-cyclodextrin, pH 3, and methanol, 80:20, in isocratic conditions, with a flow rate of 1 mL/min. To optimize quantitative analysis three wavelengths were employed for detection: λ= 198 nm, λ= 200 nm, and λ= 202 nm. SPE was applied for the extraction from urine samples of analytes. Validation parameters show recoveries always above 82%; LOD was set at 1 μg/mL with an LOQ of 3 μg/mL. The linear dynamic range (from 4 to 100 μg/mL) showed excellent correspondence. This method is rapid and inexpensive and can be applied to several samples simultaneously using a manifold for SPE extraction. The analytes were separated completely and could be fully quantified. The method was used for the analysis of urine samples from 54 workers exposed to xylene in hospital laboratories and showed a good applicability while allowing quantification even at low doses. © 2009 Elsevier B.V. Source


Caporossi L.,ISPESL National Institute of Occupational Safety and Prevention | Tranfo G.,ISPESL National Institute of Occupational Safety and Prevention | Paci E.,ISPESL National Institute of Occupational Safety and Prevention | De Rosa M.,ISPESL National Institute of Occupational Safety and Prevention | And 3 more authors.
Chromatographia | Year: 2010

An LC-DAD method was developed to determine residues of oxamyl on greenhouse workers' skin. The wipe test was used to obtain samples from hands, forearms, neck and gloves of workers taking cactus cuttings 48 h after pesticide treatment. It was validated in a concentration range 50-5,000 ng mL -1. The results on six workers and ten office employees indicated significant differences particularly in the hand samples. To avoid the overestimation owing to an interfering substance we proceeded to confirm these findings with LC-MS-MS, which found the pesticide only on the surface of the workers' gloves, proving that there was indeed some UV interference. © 2010 Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH. Source

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