IsoLife BV

Wageningen, Netherlands

IsoLife BV

Wageningen, Netherlands
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Fortier-Mcgill B.E.,University of Toronto | Dutta Majumdar R.,University of Toronto | Dutta Majumdar R.,Bruker | Lam L.,University of Toronto | And 10 more authors.
Journal of Agricultural and Food Chemistry | Year: 2017

A relatively recent technique termed comprehensive multiphase (CMP) NMR spectroscopy was used to investigate the growth and associated metabolomic changes of 13C-labeled wheat seeds and germinated seedlings. CMP-NMR enables the study of all phases in intact samples (i.e., liquid, gel-like, semisolid, and solid), by combining all required electronics into a single NMR probe, and can be used for investigating biological processes such as seed germination. All components, from the most liquid-like (i.e., dissolved metabolites) to the most rigid or solid-like (seed coat) were monitored in situ over 4 days. A wide range of metabolites were identified, and after 96 h of germination, the number of metabolites in the mobile phase more than doubled in comparison to 0 h (dry seed). This work represents the first application of CMP-NMR to follow biological processes in plants. © 2017 American Chemical Society.


Gorissen A.,IsoLife BV | Kraut N.U.,University of Groningen | De Visser R.,IsoLife BV | De Vries M.,University of Groningen | And 2 more authors.
Food Chemistry | Year: 2011

The isothiocyanate sulforaphane, present in significant amounts in broccoli (Brassica oleracea L.) seedlings in the form of its precursor glucoraphanin, has been identified as an inducer of quinine reductase, a phase-II detoxification enzyme known for its anticarcinogenic properties. Its concentration in broccoli seedlings usually decreases during the first 7-14 days after germination. No conclusive data on sulforaphane metabolism in seedlings are available in the literature. Here, we unambiguously demonstrate in 12C/ 13C-cross experiments that sulforaphane is not biosynthesised de novo during the first week of seedling development. Both 12C (99 atom% 12C) and 13C (98 atom% 13C) broccoli seeds were produced and subsequently germinated and grown either in a 13CO 2 or a 12CO 2 environment. Afterwards, the labelling degree of sulforaphane in seeds and in seedlings was analysed by HPLC-MS. We conclude that sulforaphane exclusively originates from seed reserves and that de novo biosynthesis is not detectable (<1%) in broccoli seedlings. © 2011 Elsevier Ltd. All rights reserved.


Lam L.,University of Toronto | Soong R.,University of Toronto | Sutrisno A.,University of Toronto | De Visser R.,IsoLife BV | And 15 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

Seeds are complex entities composed of liquids, gels, and solids. NMR spectroscopy is a powerful tool for studying molecular structure but has evolved into two fields, solution and solid state. Comprehensive multiphase (CMP) NMR spectroscopy is capable of liquid-, gel-, and solid-state experiments for studying intact samples where all organic components are studied and differentiated in situ. Herein, intact 13C-labeled seeds were studied by a variety of 1D/2D 1H/13C experiments. In the mobile phase, an assortment of metabolites in a single 13C-labeled wheat seed were identified; the gel phase was dominated by triacylglycerides; the semisolid phase was composed largely of carbohydrate biopolymers, and the solid phase was greatly influenced by starchy endosperm signals. Subsequently, the seeds were compared and relative similarities and differences between seed types discussed. This study represents the first application of CMP-NMR to food chemistry and demonstrates its general utility and feasibility for studying intact heterogeneous samples. © 2013 American Chemical Society.

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