Göttingen, Germany
Göttingen, Germany
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Leinemann L.,University of Gottingen | Hosius B.,ISOGEN | Kahlert K.,Thuringen Forst Anstalt Offentlichen Rechts | Kuchma O.,ISOGEN | And 3 more authors.
Allgemeine Forst- und Jagdzeitung | Year: 2013

Title of the paper: Gene marker analysis concerning hybrid formation and natural regeneration of Sorbus latifolia agg. in Thuringia.

Kleinschmit J.R.G.,Nordwestdeutsche Forstliche Versuchsanstalt | Hosius B.,ISOGEN | Leinemann L.,University of Gottingen
Forstarchiv | Year: 2012

Malus sylvestris is an endangered species and conservation measures are necessary. The main precondition to make conservation of M. sylvestris feasible is the differentiation between the wild types of M. sylvestris and the numerous cultivated forms of M. domestica. This problem is of particular relevance for the establishment of conservation seed orchards. Since morphological traits are often unsecure, due to genotype environment interaction, a set of six nuclear SSR-markers was applied to analyse the resolution between M. sylvestris and different cultivated apple trees. The results support a strong genetic differentiation between the two groups with a genetic distance of 83.5%. The assignment of single individuals with the software Structure resulted in high "membership coefficients". Genotyping 963 in situ selected putative M. sylvestris resulted in an estimate of 15% misclassificated genotypes, which represent an undesirable pollen source. The gene flow estimates into a seed orchard give values of 56% minimum of pollen contamination. Thus future seed production will be done in a seed orchard where flowering takes place under containment. © DLV GmbH.

Leinemann L.,University of Gottingen | Kahlert K.,Thuringer Landesanstalt fur Wald | Arenhovel W.,Thuringer Landesanstalt fur Wald | Voth W.,Landesforst Mecklenburg Vorpommern | Hosius B.,ISOGEN
Allgemeine Forst- und Jagdzeitung | Year: 2010

The main objective of the survey was the characterization of Hybrid-Species of the Sorbus latifolia aggr. with universal (ccmp) cpDNA markers. In total, 141 samples of 12 Sorbus species including the two ancestral species Sorbus aria and Sorbus torminalis were investigated. Beside eight botanically characterized HybridSpecies {Sorbus acutisecta, Sorbus decipiens, Sorbus heilingensis, Sorbus isenacensis, Sorbus latifolia, Sorbus multicrenata, Sorbus parumlobata, Sorbus subcordata) the species Sorbus pinnatifida and Sorbus intermedia were analysed (see Table I and Figure 1). Isozyme gene markers were applied to investigate apomictic regeneration in one species {Sorbus heilingensis) with overall 68 progenies of ten seed parents. The cpDNA analyses displayed 10 haplotypes (see Table 2). In Table 3 the haplotype frequencies within the single species are shown. Two out of a total of 10 haplotypes (S-9 and S-14) were characteristic for the ancestral species Sorbus aria and Sorbus torminals, respectively, with frequencies of about 70% each. Other haplotypes are rare in these two species. With the exception of one Sorbus latifolia tree all hybrids showed solely haplotypes of the two potential ancestral species. One of the rare haplotypes (S-22) of the ancestral species displayed frequencies higher than 50% in some of the hybrid species. Thus, a third unidentified ancestral species is possibly involved in the formation of this hybrid aggregate. The distribution of haplotypes within and between the single hybrid species indicates that hybridisation between Sorbus aria and Sorbus torminalis is possible in both directions. Each of the taxonomically distant species Sorbus pinnatifida and Sorbus intermedia showed unique haplotypes. The investigations concerning apomictic regeneration in Sorbus heilingensis revealed unexpectedly high estimates of sexual regeneration of 96% since only 4% of the progenies showed identical patterns to their particular seed parents.

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