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Purnomo Y.,Brawijaya University | Purnomo Y.,Islamic University of Malang | Soeatmadji D.W.,Brawijaya University | Sumitro S.B.,Brawijaya University | Widodo M.A.,Brawijaya University
Asian Pacific Journal of Tropical Biomedicine | Year: 2015

Objective: To evaluate the anti-diabetic potential of leaf extract from Urena lobata (U. lobata) through dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Methods: U. lobata leaf was extracted in hot water and ethanol. The activity of DPP-IV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPP-IV and vildagliptin, as standard reference. A product of the reactions between gly-pro-pnitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results: The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC50 values of 1 654.64 and 6 489.88 mg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions: The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol. © 2015 Hainan Medical University. Source

Crysdian C.,Islamic University of Malang
2010 International Conference on Distributed Frameworks for Multimedia Applications, DFmA 2010 | Year: 2010

The works on developing a Digital Elevation Model (DEM) for small scale spatial objects is presented in this report. The motivation is to visualize a small spatial object as detail as possible. Here, small scale spatial object is defined as a spatial object that has a relatively small size such as a city, a volcano, or a lake. To accomplish this task, the research was conducted in three stages i.e. elevation data retrieval, 3D visualization and data enhancements, and the last is definition of object boundary. Elevation data is obtained from SRTM dataset that has 3 arc-second or approximately 90 meters data resolution. Data obtained from SRTM is then visualized in 3D, in which visualization is enriched with view angle setting. Results of visualization show that data enhancement is necessary to be developed to have better presentation of small-scale spatial object in 3D. This paper proposes several methods namely population and neighbors average, to enhance elevation dataset. Comparison among these methods is held to choose the best method to support 3D visualization. Different view angle setting is used in this stage. After having the best method to enhance elevation data set, next stage of the work is to develop spatial object boundary. This step aims to precisely view the object of interest. To develop object boundary, a set of points in term latitude and longitude coordinate is defined and connected by a set of edges computed using linear equation. © 2010 University Sains Malaysia. Source

Romaidi,Hiroshima University | Romaidi,Islamic University of Malang | Ueki T.,Hiroshima University
Marine Biotechnology | Year: 2016

Isolation of naturally occurring bacterial strains from metal-rich environments has gained popularity due to the growing need for bioremediation technologies. In this study, we found that the vanadium concentration in the intestine of the vanadium-rich ascidian Ascidia sydneiensis samea could reach 0.67 mM, and thus, we isolated vanadium-resistant bacteria from the intestinal contents and determined the ability of each bacterial strain to accumulate vanadium and other heavy metals. Nine strains of vanadium-resistant bacteria were successfully isolated, of which two strains, V-RA-4 and S-RA-6, accumulated vanadium at a higher rate than did the other strains. The maximum vanadium absorption by these bacteria was achieved at pH 3, and intracellular accumulation was the predominant mechanism. Each strain strongly accumulated copper and cobalt ions, but accumulation of nickel and molybdate ions was relatively low. These bacterial strains can be applied to protocols for bioremediation of vanadium and heavy metal toxicity. © 2016, Springer Science+Business Media New York. Source

Crysdian C.,Islamic University of Malang
Journal of Theoretical and Applied Information Technology | Year: 2016

The paper presents an effort to standardize cost effective to support the development of spatial modeling. It is motivated by the increasing role of both cost effective and spatial modeling to contribute on society development worldwide. Therefore the future of spatial modeling would focus on minimizing cost and required time for model generation, in which cost effective become the only reliable approach to achieve these objectives. Considering that spatial modeling commonly consists of data acquisition, digitations process and model development, hence the characteristic of cost effective to minimize any required resources for system development is required to be presented in each stage. It means the method being utilized in each stage must comply with the objective of cost effective, thus method selection takes a vital role in the development process. The work has presented various strategies consisting of different methods being employed for model development in which each carries its own benefits and flaws. Finally it is important to consider available resources to meet with method selection in order to enable wise implementation of spatial modeling. © 2005-2016 JATIT & LLS. All rights reserved. Source

Ueki T.,Hiroshima University | Yamaguchi N.,Hiroshima University | Romaidi,Hiroshima University | Romaidi,Islamic University of Malang | And 2 more authors.
Coordination Chemistry Reviews | Year: 2015

Several families of ascidians accumulate extremely high levels of vanadium in their blood cells. The concentration of vanadium has been determined in each species; the highest concentration, found in Ascidia gemmata, reaches 350mM, corresponding to 107 times that of sea water. How and why ascidians accumulate vanadium in a highly selective manner and at such extremely high levels have yet to be determined. To address these questions, our research group sought to identify the genes and proteins responsible for the accumulation and reduction of vanadium in vanadocytes, a type of blood cell, as well as the process of vanadium transport from sea water to blood cells through the branchial sac, intestine, and blood plasma. Here, we review the accumulation steps as a system, especially those related to the concentration and chemical species of vanadium at each step. A comprehensive analysis on each organ has already revealed several categories of protein families, such as vanadium-binding proteins and vanadium transporters. Herein, we also discuss the mechanisms by which ascidians selectively accumulate vanadium ions from a biochemical viewpoint. © 2014 Elsevier B.V. Source

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