Shaer A.,Islamic Azad UniversityFars |
Shaer A.,Shiraz University of Medical Sciences |
Azarpira N.,Shiraz University of Medical Sciences |
Karimi M.H.,Shiraz University of Medical Sciences |
And 2 more authors.
Experimental and Clinical Transplantation | Year: 2016
Objectives: Diabetes results from inadequate insulin production from pancreatic ß-cells. Islet cell replacement is an effective approach for diabetes treatment; however, it is not sufficient for all diabetic patients. Thus, finding a new source with effective maturation of ß-cells is the major goal of many studies. MicroRNAs are a class of small noncoding ribonucleic acid that regulate gene expression through posttranscriptional mechanisms. MicroRNA-7 has high expression level during pancreatic islet development in humans, thereby playing a critical role in pancreatic β-cell function. We study aimed to develop a protocol to differentiate human-induced pluripotent stem cells efficiently into isletlike cell clusters in vitro by using microRNA-7. Materials and Methods: Human-induced pluripotent stem cell colonies were transfected with hsamicroRNA-7 by using siPORT NeoFX transfection agent. Total ribonucleic acid was extracted 24 and 48 hours after transfection. The expression of transcription factors which were important during pancreases development was also performed. On the third day, the potency of the clusters was assessed in response to high glucose levels. Diphenylthiocarbazone was used to identify the existence of the ß-cells. The presence of insulin and Neurogenin-3 proteins was investigated by immunocytochemistry. Results: Morphologic changes were observed on the first day after chemical transfection, and cell clusters were formed on the third day. The expression of pancreatic specific transcription factors was increased on the first day and significantly increased on the second day. The isletlike cell clusters were positive for insulin and Neurogenin-3 proteins in immunocytochemistry. The clusters were stained with Diphenylthiocarbazone and secreted insulin in a glucose challenge test. Conclusions: MicroRNA-7 transcription factor net-work is important in pancreatic endocrine differe-ntiation. Chemical transfection with microRNA-7 can differentiate human induced pluripotent stem cells into functional isletlike cell clusters in a short time. © Başkent University 2016 Printed in Turkey. All Rights Reserved.
Zarezadeh R.,Islamic Azad UniversityFars |
Azarbayjani M.A.,Islamic Azad University at Tehran
Diving and Hyperbaric Medicine | Year: 2014
Introduction: Environmental pressure changes with depth may lead to changes in various hormone levels in the body. Of interest are the so-called stress hormones, such as cortisol. Other factors altering cortisol levels are anxiety, exercise and cold. We investigated serum cortisol changes after air scuba dives in 24–27ºC open water up to a depth of 30 metres. Method: Ten, experienced, male divers participated in the study. Four dives, to depths of 1, 10, 20 and 30 metres’ sea water (msw) for 20 minutes bottom time, at rest, were conducted at about 1000 h on four consecutive days in the Persian Gulf. Before diving and soon after surfacing, approximately 5 ml blood was drawn from a right antecubital vein for serum cortisol assay, using a radioactive immunoassay technique. Repeated measures was used to analyse cortisol changes with depth. Results: There were significant differences in the pre-dive cortisol levels (df = 1, F = 5.978, P < 0.037) and post-dive levels (df = 1, F = 34.567, P < 0.001). Cortisol levels increased with immersion irrespective of depth compared to pre-dive levels, whilst they were further significantly raised after dives to 10 m (mean 312.6 nmol∙L-1), 20 m (mean 299.1 nmol∙L-1) and 30 m (mean 406.7 nmol∙L-1) depth compared to levels after the 1 m dive (mean 189 nmol.L-1). Conclusion: The observed changes in serum cortisol were considered to be the result of the physiological effects of immersion combined with increased environmental pressure, rather than resulting from anxiety, heavy exercise or cold stress. © 2014, South Pacific Underwater Medicine Society. All rights reserved.
Afshari A.,Islamic Azad UniversityFars |
Yaghobi R.,Shiraz University of Medical Sciences |
Karimi M.H.,Shiraz University of Medical Sciences |
Darbouy M.,Islamic Azad UniversityFars |
And 4 more authors.
Experimental and Clinical Transplantation | Year: 2015
Objectives: Cytomegalovirus (CMV) establishes a lifelong, asymptomatic infection in immunocompetent hosts. Interleukin-17 producing CD4+ T-cells (Th-17) are a subtype of CD4+ T-cells. The precise role of Th-17 responses during cytomegalovirus replication has not been elucidated, although recent studies suggest that infections such as murine cytomegalovirus induce a Th-17 response. Th-17 cells also have been associated with allograft rejection and autoimmune diseases. In this study, we tried to find the relation of cytomegalovirus infection and interleukin 17 (IL-17) cytokine in liver-transplanted patients. Materials and Methods: Two groups of patients were evaluated in this study. The first group consisted of 54 cytomegalovirus uninfected livertransplanted patients, and the second group consisted of 15 cytomegalovirus-infected patients. Three ethylenediaminetetraacetic acid-treated blood samples were collected from each patient on days 1, 4 and 7 post liver transplant. For diagnosing cytomegalovirus infection antigenemia and Taq- Man real-time polymerase chain reaction protocols were used. Also, to determine the expression level of IL-17 gene, an in-house SYBR green real-time polymerase chain reaction technique was used. Results: Using antigenemia and also Taq-Man realtime polymerase chain reaction helps find active cytomegalovirus infection, and the load of cytomegalovirus in each patient. The first group of patients showed that IL-17 expression level was down-regulated after day 4 of sampling. But in cytomegalovirus-infected patients, IL-17 expression level was increased significantly. The results between IL-17 gene expression level between the 2 groups of patients showed that IL-17 expression level significantly increased in second group during day 4 (P = .038) and 7 (P = .009) postliver transplant. Conclusions: Significant increase of IL-17 mRNA levels in cytomegalovirus-infected group compared with the uninfected one reinforced the role of IL-17 as a proinflammatory cytokine dealing with cytomegalovirus infection in liver transplanted patients. © Başkent University 2015 Printed in Turkey. All Rights Reserved.
Valipour Chahardahcharic S.,Islamic Azad UniversityFars |
Hoseini S.E.,Islamic Azad UniversityFars |
Vahdati A.,Islamic Azad UniversityFars |
Kesmati M.,Shahid Chamran University
Advances in Environmental Biology | Year: 2014
Background and Objectives: Despite the wide spread use of zinc oxide nanoparticles (Nano zinc oxide) in the medical and industry, a comprehensive study has been done on the effects of neuropsychological and cognitive its related processes. Considering the oxidative effects of nanoparticles, there has been a great challenge on the way to their medicinal use. The purpose of this study was to investigate the effect of acute application of Nanozinc oxide on memory processes in the presence and absence of vitamin C as an antioxidant in the adult male rats.Methods: Adult male wistar rats (200-250g) were divided into groups with 7 in each including saline, three groups received Nano zinc oxide (1.25, 2.5 and 5mg/kg), three groups received vitamin C (30, 60, 120mg/kg) alone and three groups received vitamin C and Nanozinc oxide in combination. Memory was assessed by Step Down device after teaching using passive avoidance learning.Results: It was revealed that in all three doses of Nano zinc oxide there was a significant loss of memory (P <0.001and P <0.01) while no change in locomotor activity. Vitamin C as antioxidants, at doses of 60 and 120 mg impaired memory and in 30 mg was ineffective. Ineffective dose of vitamin C could not improve memory deficit resulting from the Nano zinc oxide.Conclusion: Considering the results of this study, acute application of Nano zinc oxide causes significant impairment in long-term memory, and this effect is probably not just due to its oxidative effect. This action mechanism may be effect through change in receptors activity or neurotransmitters releasing which is associated with memory that needs to be investigate further. © 2014 AENSI Publisher All rights reserved.
Nekoueizadeh M.,Islamic Azad UniversityFars |
Motamedi A.,Shiraz University of Medical Sciences
International Journal of Applied Linguistics and English Literature | Year: 2013
Academicians are identified with their papers and expertise in writing scholarly articles, either for promotion or for satisfying their prestige. Iranian academic members are expected to win a justifiable stance by the quality and quantity of their publications and presentations. Regrettably through pervious studying about second language writing, any studies haven’t been dedicated to the style of writing articles, which are used by academic members. Former studies on second language writing indicate that style in academic paper writing is most likely ignored. The purpose of this study is to explore the role of mind translation strategy among Iranian academic members for expressing their own opinion through writing second language academic papers. The present paper has based its hypothesis on three levels of strategies, effective in writing academic papers, namely: 1-Do Iranian academicians follow specific strategies in writing their academic papers? 2-What role does translation play as a strategy in their writing academic papers? 3-Do they feel a need for a strategy shift in their academic paper writing? Data elicited based on survey and corpora analysis in form of CBDTS- on micro and macro levels, are put into matrices and their analyses are supportive of academicians’ reliance on different types of mental translation use and their shift toward authentic writing after receiving feedback from their reviewers. © Australian International Academic Centre, Australia.
Fallahi A.A.,Shiraz University |
Shekarfroush S.,Islamic Azad UniversityFars |
Rahimi M.,University of Kashan |
Jalali A.,Isfahan University of Technology |
Khoshbaten A.,Tehran University of Medical Sciences
Iranian Journal of Basic Medical Sciences | Year: 2016
Objective(s): High-intensity interval training (HIIT) increases energy expenditure and mechanical energy efficiency. Although both uncoupling proteins (UCPs) and endothelial nitric oxide synthase (eNOS) affect the mechanical efficiency and antioxidant capacity, their effects are inverse. The aim of this study was to determine whether the alterations of cardiac UCP2, UCP3, and eNOS mRNA expression following HIIT are in favor of increased mechanical efficiency or decreased oxidative stress. Materials and Methods: Wistar rats were divided into five groups: control group (n=12), HIIT for an acute bout (AT1), short term HIIT for 3 and 5 sessions (ST3 and ST5), long-term training for 8 weeks (LT) (6 in each group). The rats of the training groups were made to run on a treadmill for 60 min in three stages: 6 min running for warm-up, 7 intervals of 7 min running on treadmill with a slope of 5° to 20° (4 min with an intensity of 80-110% VO2max and 3 min at 50-60% VO2max), and 5-min running for cool-down. The control group did not participate in any exercise program. Rats were sacrificed and the hearts were extracted to analyze the levels of UCP2, UCP3 and eNOS mRNA by RT-PCR. Results: UCP3 expression was increased significantly following an acute training bout. Repeated HIIT for 8 weeks resulted in a significant decrease in UCPs mRNA and a significant increase in eNOS expression in cardiac muscle. Conclusion: This study indicates that Long term HIIT through decreasing UCPs mRNA and increasing eNOS mRNA expression may enhance energy efficiency and physical performance. © 2016, Mashhad University of Medical Sciences. All rights reserved.
Ghaedi A.M.,Islamic Azad University at Gachsaran |
Ghaedi M.,Yasouj University |
Pouranfard A.R.,Yasouj University |
Ansari A.,Islamic Azad UniversityFars |
And 7 more authors.
Journal of Molecular Liquids | Year: 2016
Rapid adsorption of Triamterene using multi-walled carbon nanotube (MWCNT) and single-walled carbon nanotube (SWCNT) was well investigated and elucidated. The impact of influential variables such as temperature, amount of adsorbent, initial drug concentration, contact time was modeled using multiple linear regression (MLR) and artificial neural network (ANN) and the influential variables were optimized using genetic algorithm (GA). The adsorption equilibrium and kinetic data was well fitted and found to be in good agreement with the Langmuir monolayer isotherm model and pseudo second order kinetics mechanism. The maximum adsorption capacity of SWCNT and MWCNTs for the removal of Triamterene was found to be 25.77 mg g- 1 and 33.14 mg g- 1 respectively. The negative value of adsorption enthalpy (ΔH°) reveals towards the exothermic nature of the adsorption process. Based on the results comparison of the proposed models, results revealed that the applicability of the ANN model is more appropriate in comparison to the MLR model for predicting the adsorption efficiency of the process. The coefficient of determination (R2) of 0.980 and mean squared error (MSE) of 0.002 for adsorption on SWCNT 0.986 and 5.4e-04 on MWCNT were obtained, respectively using the optimal ANN model. © 2016 Elsevier B.V. All rights reserved.
Shayganmehr F.-S.,Shahid Beheshti University of Medical Sciences |
Shayganmehr F.-S.,Islamic Azad UniversityFars |
Alebouyeh M.,Shahid Beheshti University of Medical Sciences |
Azimirad M.,Shahid Beheshti University of Medical Sciences |
And 3 more authors.
Iranian Biomedical Journal | Year: 2015
Background: Reduced susceptibility of Clostridium difficile to antibiotics is problematic in clinical settings. There is new evidence indicating the cotransfer of toxin-encoding genes and conjugative transposons encoding resistance to antibiotics among different C. difficile strains. To analyze this association, in the current study, we evaluated the frequency of toxigenic C. difficile among the strains with different multidrug-resistant (MDR) profiles in Iran. Methods: Antimicrobial susceptibility patterns and minimal inhibitory concentrations (MIC) of the isolates were determined against metronidazole, imipenem, ceftazidime, amikacin, and ciprofloxacin by agar dilution method. The association of the resistance profiles and toxigenicity of the strains were studied by PCR targeting tcdA and tcdB genes. Results: Among 86 characterized strains, the highest and lowest resistance rates were related to ciprofloxacin (97%) and metronidazole (5%), respectively. The frequency of resistance to other antibiotics was as follow: imipenem (48%), ceftazidime (76%), and amikacin (76.5%). Among the resistant strains, double drug resistance and MDR phenotypes were detected in the frequencies of 10.4% and 66.2%, respectively. All of the metronidazole-resistant strains belonged to tcdA +/tcdB + genotype with triple or quintuple drug resistance phenotypes. MIC50 and MIC90 for this antibiotic was equally ≤ 8 μg/ml. Conclusion: These results proposed the association of tcdA +/tcdB + genotype of C. difficile and the emergence of resistance strains to broad-spectrum antibiotics and metronidazole. © 2015, Pasteur Institute of Iran. All rights reserved.
Hassanzadeh Y.,Islamic Azad UniversityFars |
Bahador N.,Islamic Azad UniversityFars |
Baseri-Salehi M.,Islamic Azad University
Iranian Journal of Microbiology | Year: 2015
Background and Objective: Photobacterium damselae subsp. damselae is a marine pathogenic bacterium which causes disease in marine animals and human. This bacterium mostly found in coastal shallow seawater. So, the aim of this study was isolation and characterization of Photobacterium damselae subsp. damselae from edible fish of Persian Gulf, Bandar Abbas. Material and Methods: Totally 100 fish from different species were evaluated and out of that 5 different types of fish with external symptoms including: Caranx sexfasciatus, Lethrinus olivaceus, Scomberoid tol, Auxis thazard and Liza macrolepis, were collected from Bandar Abbas local fish market in September 2013. The samples were cultured on Marin Agar 2216 and Thiosulfate Citrate Bile salts Sucrose Agar media and incubated at 25°C for 48 hrs. Then the isolates were characterized using biochemical (API 20 NE system) and molecular techniques. In addition, antibiotic susceptibility, presence of poly ß hydroxy butyrate and hemolysis activity of isolates were evaluated. Results and Conclusion: Entirely, 30 Gram negative bacterial colonies were isolated from the selected fish. Among the isolates, two suspected colonies were identified as Photobacterium damselae from Caranx sexfasciatus with API 20NE biochemical test. This results confirmed by 16s rRNA sequencing method. Both isolates showed a hemolytic with existence of ß hydroxyl butyrate. Furthermore, the isolates were susceptible to ciprofloxacin, chloramphenicol and nalidixic acid. Conclusion: Overall, the study indicated first time isolation of this bacterium from one type of fish caught from Persian Gulf, which warns us to pay more attention to fishery in this geographical area. © 2015, Tehran University of Medical Science. All rights reserved.
Ghaedi M.,Yasouj University |
Ansari A.,Islamic Azad UniversityFars |
Nejad P.A.,Islamic Azad UniversityFars |
Ghaedi A.,Islamic Azad University at Gachsaran |
And 2 more authors.
Environmental Progress and Sustainable Energy | Year: 2015
The objective of this work is the study of adsorption of Eosin B by cobalt oxide nanoparticle loaded on activated carbon (Co2O3-NP-AC). This new material with high efficiency in a routine manner was synthesized in our laboratory, and its surface properties such as surface area, pore volume, and functional groups were characterized with different techniques such X-ray diffraction, Brunauer, Emmett, and Teller, and scanning electron microscopy analysis. The effect of solution pH, adsorbent dosage (0.005-0.02 g), contact time (0.5-30 min), and initial concentration of dye (30-80 mg L-1) on the adsorption process was investigated. Thus, Langmuir, Freundlich, Tempkin, and D-R isothermal models are applied for fitting the experimental data, and the data well presented by Langmuir model with a maximum adsorption capacity of 588.2 mg g-1 at 25°C. Kinetic studies at various adsorbent dosage and initial Eosin B concentration show that maximum Eosin B removal was achieved within 18 min of the start of every experiment at most conditions. The combination of pseudo-second-order rate equation and intraparticle diffusion model (with removal more than 99%) is usable to explain the experimental data of adsorption process at all conditions. The influences of parameters including initial dye concentration, adsorbent dosage (g), and contact time on Eosin B adsorption onto cobalt oxide nanoparticles loaded on AC were investigated by multiple linear regression (MLR) and artificial neural network (ANN), and the influences of variables were optimized using Bees Algorithm. Comparison of the results obtained using introduced models showed the ANN model is better than the MLR model for prediction of Eosin B removal using cobalt oxide nanoparticles loaded on AC. Using the optimal ANN model, the coefficients of determination (R2) were 0.9965 and 0.9936; mean squared error values were 0.00015 and 0.00029 for training and testing data, respectively. © 2014 American Institute of Chemical Engineers.