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Vaniyambadi, India

Exposure to pyrethroid pesticides has been associated with adverse neurodevelopmental outcomes, like neurodegenerative disorder, low IQ, pervasive developmental disorder, attention problems. Thus, we investigated the relationship between pyrethroid deltamethrin exposure to acetylcholine esterase, ATPase, oxidative stress biomarkers, and impaired behavior performance, and the possible ameliorating mechanism of dietary flavonoid naringin in male Wistar rats. Adult male wistar rats were divided into four different groups. Group I: control group; group II received DLM dissolved in corn oil 12.8mg/kg BW orally (1/10 LD50) for three weeks; group III received DLM as group II and naringin (100mg/kg BW for 21days) orally. Group IV: naringin alone. DLM exposure leads to reduction in the levels of acetylcholinesterase, Na+/K+, Ca2+, Mg2+ ATPase, enzymic and non-enzymic antioxidants activities in cortex and hippocampus region and increase the activities of TBARS. DLM-induced neuronal alterations was evidenced by impairment behavioral performance, like memory, anxiety, locomotor, and emotionality behaviors. This is also supported by histopathological findings of cortex and hippocampus region of rats. However, naringin treatment modulates the abnormalities of DLM-induced alterations in oxidative stress biomarkers, acetylcholine esterase, ATPase, and behavioral performance of rats. These findings highlight the efficacy of naringin as neuroprotectant. In conclusion, our results demonstrated that DLM cause neurobehavioral and biochemical alterations. Oxidative stress, free radical mechanism play major role on DLM-induced neurotoxicity. Naringin could be a suitable agent for preventing the toxicity of DLM by its potent antioxidant, free radical scavenging and neuroprotective activity. © 2014.


Ahmed N.S.,Islamiah College Autonomous | Jaffar Ali H.A.,Islamiah College Autonomous
Mitochondrial DNA | Year: 2015

The mitochondrial cytochrome c oxidase subunit I (COI) gene, a widely accepted molecular marker for species identification and classification, has been questioned because of the presence of Numts. In this study we found the presence of Numts in the COI chromatogram of two tunicates, Polyclinum indicum and Polyclinum madrasensis belonging to the genus Polyclinum. Numts were also present in our sequence (Accession Number: KJ944391) and in other sequences belonging to genus Polyclinum in the GenBank record. The GeneBank database of genus Polyclinum contains COI-like sequences and COI pseudogenes, but no record of COI gene from Polyclinids. The prevalence of Numts in Polyclinids belonging to Tunicata, is an impediment to DNA barcoding studies of Polyclinum species. © 2015 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted


Magendira Mani V.,Islamiah College Autonomous | Gokulakrishnan A.,Islamiah College Autonomous
Biomedicine and Preventive Nutrition | Year: 2014

The protective effect of naringin on deltamethrin poisoning in human erythrocyte was studied using an in vitro model. Hemolysis, percentage met-hemoglobin, lipid peroxidation, glutathione, antioxidant enzymes and erythrocyte ghost protein pattern were assessed to investigate the effect of naringin. Erythrocytes at a hematocrit of 10% were incubated with 500 ppm of deltamethrin and/or 0.1. M naringin under physiological conditions of temperature and pH for 2 h. Deltamethrin significantly increased the percentage of hemolysis and met-hemoglobin in human erythrocytes as compared to the control erythrocytes and naringin significantly (P< 0.05) inhibited the percentage of hemolysis and met-hemoglobin. The levels of lipid peroxides and conjugated diene increased whereas the level of glutathione decreased significantly (P< 0.05) by deltamethrin-incubated erythrocytes. Naringin significantly inhibited the formation of lipid peroxides and conjugated diene while increased the glutathione level in erythrocytes incubated with deltamethrin. The activity of antioxidant enzymes and non-enzymic antioxidants were decreased in erythrocytes incubated with deltamethrin whereas naringin improved the activities of these antioxidant and non-enzymic antioxidants. SDS-PAGE of erythrocyte ghost protein pattern showed an alteration in the protein bands by deltamethrin poisoning but naringin significantly inhibited the alteration in protein profile. The present study divulges that naringin can reduce the abnormalities of deltamethrin poisoning by ameliorating oxidative stress. This finding raises the possibility that naringin may provide protection from pesticide poisoning. © 2013.


Tariq N.P.M.M.,Islamiah College Autonomous | Ifham S.M.R.,Islamiah College Autonomous
Research Journal of Pharmacy and Technology | Year: 2013

An ethno botanical survey was conducted in Yelagiri hills, located in Vellore district, Tamil Nadu (State), India. The purpose of this study is to collect data about medicinal plants available in yelagiri hills in order to preserve its rich bio resources through documentation since there is scarce information about the existence of medicinal plants in this region. This survey was conducted based on semi structured interviews, field visits and participatory observations. A total of 28 medicinal plants were identified and confirmed in herbarium of university of madras, Chennai. Plant species belonging to diverse families like Lilaceae, Solanaceae, Euphorbiaceae followed by Lamiaceae, apocyanaceae were found. These plants have medicinal properties including anticancer, antidiabetic, antidote, antibacterial, anthelminthic, antispasmodic, antianalgesic, cure for epilepsy, diarrhea, weaknesses etc. However, certain medicinal plants were found to be in vulnerable condition like Ocimum sanctum and Leucas aspera. This could be due to growth of herbicide parthenium species. The presence of this herbicide has led to the decline of certain medicinal plants in this area. Further investigation is required to preserve the bioresources that is slowly declining in this region. © RJPT All right reserved.


Magendira Mani V.,Islamiah College Autonomous | Asha S.,DKM College for Womens Autonomous
Biomedicine and Aging Pathology | Year: 2014

Deltamethrin (DEL) is a type II α - cyano group containing synthetic pyrethroid insecticide that is used extensively for controlling flies, mosquitoes, insects, pests worldwide. DEL exposure leads to pathophysiology of a broad spectrum of cerebrovascular & neurodegenerative disorders like Parkinson's, Lou Gehrig's disease, Alzheimer's disease, developmental deficits, birth defects, and learning disabilities. In these studies, we have demonstrated that the deltamethrin-induced neurotoxicity and ameliorating effect of dietary flavonoid naringin by its antioxidant and neuro-protective ability in male wistar rat. Adult male wistar rats were divided into four different groups. Group I vehicle treated control group; group II received deltamethrin dissolved in corn oil 12.8 mg/kg BW orally (1/10 LD 50) for three weeks (21 days) to induce neurotoxicity; group III received naringin (100 mg/kg BW for 21 days) orally. Group IV naringin alone treated. DEL-induced neurotoxicity was evidenced by increased activities of creatine phosphokinase, lactate dehydrogenase, TBARS in DEL administered rat brain tissue homogenate, and decrease activity of acetylcholinesterase (AChE) activities, however it was reversed by naringin treatment. DEL administered rats showed reduction in the levels of enzymic (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase) and non-enzymic antioxidants (glutathione, vitamin C and vitamin E) levels. However, normalized antioxidant and non-enzymic antioxidant defenses were reported in the naringin treated rats. These findings highlight the efficacy of naringin as a neuro-protectant in DEL-induced neurotoxicity which is also supported by native gel electrophoresis, agarose gel electrophoresis, histopathological studies of rat brain tissue. © 2013 Published by Elsevier Masson SAS.

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