News Article | May 10, 2017
WASHINGTON, DC, May 10, 2017 /24-7PressRelease/ -- It's time to give back to the woman that gave you everything. There's something about giving a realm of beauty to the special women in our lives that touches the hearts in all of us. No one said shopping for mom had to be difficult or boring! Enjoy this Saturday afternoon with fine tunes, a glass of bubbly, a taste of culinary delight and a fantastic collaborative of 35 hand-selected brands you would typically only find online, right here in Shaw's very own WonderBread building, home of WeWork, ISL and non-profit, Youth For Understanding. Take this pre-mother's day as a celebration of women, all women! Poppir.com (Women In the Arts) will be hosting this free event and encourage all to bring their friends and family as this weekend kicks off D.C.'s most unique Mother's Day experience! The Goods Park Avenue Pieces - Cashmere & Pearls - Quavaro Bags - Romia's Boutique - LaLaLu Mexican Art - MM Boutique - Niani - Mibbie - CoupCoup - Peacesake Candle - Dennison Lane - Casa Da Intimates - Olivia Rose Skincare - Jsquared Candle Co. - Naava Cosmetics - Chicasual - TEN Clothing - Sybarite Soul - Leyu Ambare - Swoon For - So Me Design - Whistle and Wild - The Pearl Girl - Lahara- Inlustris Creations - Ankara Streets The Mother's Day pop-up ma WonderBread Building is located at 641 S St NW, Washington, DC 20001. Don't miss it! For more information, visit the event page: Mother's Day Pop-up Market Poppir is committed to connecting emerging and established companies to short-term commercial rental space. With a steadily growing inventory of diverse properties, Poppir strives to be the premier centralized marketplace for under-utilized real estate. Through unwavering commitment to accessibility, Poppir makes properties that once felt unattainable a reality for business owners, big and small. To start your own pop-up experience, please visit us online at poppir.com.
News Article | May 22, 2017
Revel Nevada, a new, refined senior community, announces plans to offer innovative dining experiences, best-in-class amenities and unsurpassed wellness services when it opens later this year. Revel is partnering with Sodexo, world leader in Quality of Life services, and acclaimed chef and Food Network star, Beau MacMillan to offer an unparalleled dining program. Residents will be able to choose from innovative seasonal menus in the modern-American restaurant and classic favorites with a twist in the pub. In addition to inspired dishes and exceptional service, Revel will also have a liquor license so residents can unwind with their favorite drink after an active day. Both the restaurant and the pub can be enjoyed as part of an inclusive monthly meal plan. For the ultimate in flexibility, residents can also choose when and where they want to eat. Whether that’s in their apartment, in either of the restaurants, or even in a private dining room. Before or after a good meal, residents can take part in maintaining an active and healthy lifestyle, which is an integral part of being a resident at Revel. The community will offer a dual-zoned fitness center with a Zen Zone designed for low impact exercise and an Active Zone focusing on cardio and strength training. Other wellness-oriented amenities include a full-service salon, a relaxing spa and a heated saltwater pool and spa. Revel is being developed by The Wolff Company and will offer 146 one- and two-bedroom independent living residences. In addition to groundbreaking dining and wellness services, the community will feature strikingly sophisticated amenity spaces include resort-style outdoor living areas, an arts studio, a multi-purpose room and a grand living room for interacting with friends, family and neighbors. Through ISL’s award-winning Vibrant Life® program, residents can experience an expanded active lifestyle with projects and activities that contribute to their overall health and well-being. The Revel pre-leasing office is located at 43 S Stephanie Street, Suite 140 in Henderson and is open 7 days a week from 9 am to 6 pm. For more information, visit revelnevada.com or call (702) 564-6000. About Revel Located in Henderson, NV Revel is scheduled to open later in 2017. It will provide the finest in independent living for residents over 55 and older in the area. It is operated by Integral Senior Living, which manages senior living communities. ISL is founded on a care philosophy that fosters dignity and respect for residents and promotes their independence and individuality. For more information visit http://www.revelnevada.com. About ISL Integral Senior Living headquartered in Carlsbad, CA, manages a progressive selection of senior residences to meet the growing needs of today’s aging population. It currently manages 60 independent, assisted living and memory care properties throughout, Arizona, California, Colorado, Florida, Georgia, Indiana, Kansas, Kentucky, Michigan, Missouri, Nevada, Oklahoma, Oregon, South Carolina, Tennessee, Texas, Utah and Washington. It is ranked the 17th largest senior living provider in the U.S. according to Senior Housing News. ISL is founded on a care philosophy that fosters dignity and respect for residents and promotes their independence and individuality. The dedicated staff at each community is trained to maintain the highest standards of senior care services. About The Wolff Company Wolff has invested in, acquired and developed high-quality multifamily assets for more than six decades. The Company is headquartered in Scottsdale, Arizona and maintains offices in Washington, Massachusetts and California. Contact us at 480.315.9595 or visit us online at http://www.awolff.com. About Sodexo Sodexo delivers more than 100 services across North America that enhance organizational performance, contribute to local communities and improve quality of life. The Fortune Global 500 company is a leader in delivering sustainable, integrated facilities management and foodservice operations.
News Article | May 19, 2017
Integral Senior Living (ISL), a premier senior living management company, created a program that adds vibrancy to resident life. Throughout the 60 communities it manages, residents take part in Vibrant Life®, an innovative program designed to enhance and enrich residents’ lives with well-rounded and meaningful experiences. Recently a resident at ISL community Shavano Park Senior Living took part in a lifelong dream. A component of Shavano Park’s Vibrant Life program is ‘Livin’ the Dream,’ which gives residents an opportunity to try something they’ve always wanted to do. Shavano Park resident Dorothy Williams took to indoor skydiving, as her dream became a reality. “Our mission is ‘to optimize health and well-being by passionately promoting a Vibrant Life® filled with joyful experiences, meaningful endeavors, and abundant opportunities so that our residents connect with family, friends and the local community.' Mrs. Williams certainly helped us accomplish our goal,” said Collette Valentine-Gray, CEO/COO of Integral Senior Living. With the help of her granddaughter, 92-year-old Dorothy Williams was ready to fly. She slipped into her royal blue and red flight suit, zipping it all the way up to her neck before donning a shiny blue helmet – her silver hair peeking out of the bottom. It was going to be a good day to fly. Mrs. Williams was a little scared but also excited. She traded jokes with her instructor as she learned the safety rules and basics of indoor skydiving at iFly. Mrs. Williams has been a resident at Shavano Park Senior Living for about a year where dementia and stage 4 cancer haven’t slowed her down. “She’s always on the go, she has a lot of friends,” said Shavano Park’s memory care nurse, Ashley Shipman. “Almost every day she’s going to lunch with someone. She has been able to still have a very full social life here.” For this special event, Mrs. Williams’ son, Brian attended and her daughter-in-law and granddaughter joined in by taking the class along with her. “I think it eased her fears to have them along,” Ms. Shipman added. The three generations of Williams’s women sat through the general instruction for flying in the vertical air tube. Each dive lasts just a couple of minutes. Mrs. Williams flew twice, having her daughter-in-law and her granddaughter fly with her made it that much better. “I think it was good that her family was doing it with her, she was comfortable, and she had a huge smile on her face the whole time,” adds Van Orman. Mrs. Williams waving at everyone, smiling and joking around while other residents from Shavano Park’s memory care sat along and watched in awe. Today Dorothy can be seen around the community sporting her iFly t-shirt from time to time. About ISL Integral Senior Living headquartered in Carlsbad, CA, manages a progressive selection of senior residences to meet the growing needs of today’s aging population. It currently manages 60 independent, assisted living and memory care properties throughout, Arizona, California, Colorado, Florida, Georgia, Indiana, Kansas, Kentucky, Michigan, Missouri, Nevada, Oklahoma, Oregon, South Carolina, Tennessee, Texas, Utah and Washington. It is ranked the 17th largest senior living provider in the U.S. according to Senior Housing News. ISL is founded on a care philosophy that fosters dignity and respect for residents and promotes their independence and individuality. The dedicated staff at each community is trained to maintain the highest standards of senior care services. For more information about ISL, visit Integral Senior Living’s website, blog and Facebook page.
News Article | May 5, 2017
Stonecrest of Town & Country, a new assisted living and memory care community, has completed construction and is pleased to announce it’s officially welcoming residents into their new home. It is located at 1020 Woods Mill Road, in Town & Country and is committed to providing a warm and nurturing environment combined with technology, services, and amenities that make this new community a stand out in the area. Stonecrest Town & Country is managed by Integral Senior Living (ISL). “We are so pleased to open our doors to the community of Town & Country. Our goal of providing the best senior living care in a beautiful and unique environment is now a reality,” said Linda Liken-Robertson, executive director of Stonecrest of Town & Country. “We welcome everyone to come by and see what makes our community so unique and special.” Inspired by the warmth and prominence of the area, Stonecrest of Town & Country sets a new standard in senior living. Residents enjoy beautifully appointed suites, a calendar full of activities and outings, an award winning dining program and much more all within a vibrant community full of life. The upscale, elegant community features a variety of outdoor living spaces, theater, fitness center, spa, library, and more. Restaurant style “Dining by Design” includes three chef-prepared meals daily and an abundance of delicious snacks, as well as a Bistro and Coffee Bar. Overall Stonecrest of Town & Country offers 86 beautifully finished apartments, featuring 60 assisted living and 26 memory care including studio suites, one and two bedroom suites. About Stonecrest of Town & Country Stonecrest of Town & Country provides the finest in senior living with assisted living and memory care options for residents. Located in Town & Country, Missouri, the expertly trained staff will provide residents with the highest standards of senior care services amidst a beautiful community. It is operated by Integral Senior Living, which manages independent living, assisted living, and memory care properties. ISL is founded on a care philosophy that fosters dignity and respect for residents and promotes their independence and individuality. For more information visit http://www.StonecrestOfTownAndCountry.com or call (636) 527-4444. # # #
News Article | May 1, 2017
Advanced Software Products Group Inc. (ASPG) (Booth 613) will be showcasing its newest release of ReACT [v5.0] for self-service password reset and synchronization for the enterprise. ASPG will also be introducing OAR, Offline Access Recovery, which provides device access without a network connection, and ProACT, which organizes, centralizes and simplifies user provisioning and reporting. Avatier (Booth 415) will showcase the new features in the latest version of their Avatier Identity Management Suite. This release includes the ability to integrate with MFA vendors such as Duo Security, Symantec VIP, and Google Authenticator. RESTful API's, Mobile Workflow Approver, Proactive Workflow Approver Management, and Entitlement Reconciliation are included to streamline and increase usability. Alemba (Booth 520) will be showcasing Nano, our new, no-plugin interface. Nano is all about user experience and getting the job done easily. Each element, from looks to ergonomics, has been obsessively designed to deliver a slick, effortless experience empowering first-time and occasional users. Atlassian (Booth 421) will be showcasing JIRA Service Desk, a beautifully simple service desk that's easy to use, simple to setup, and has everything you need for IT support and customer service. Stop by the booth to learn why JIRA Service Desk is recognized as a leader in service desk software by G2Crowd, ahead of legacy vendors like BMC and ServiceNow. Axios Systems (Booth 507) will showcase new features including a sleek new interface, robust reporting wizard and improved workflow capabilities that have been introduced in the latest version of their IT Service Management solution, assyst, the world's first ITSM and ITAM solution to be accredited for all 16 ITIL processes. Bomgar (Booth 501) will demonstrate the newest version of Bomgar Vault, its enterprise password management solution. The newest version, which features an integration with Bomgar Remote Support and Bomgar Privileged Access, allows users to inject credentials into end servers and systems with just one click. ComAround (Booth 223), specialist and global leader in knowledge management and self-service, will demonstrate the latest KCS-related features in ComAround Knowledge™, and how to automate and enhance your support delivery by capturing, structuring and sharing knowledge. EasyVista (Booth 403) will preview powerful self-help innovations within its proven ITSM platform, including intelligent self-guided knowledge processes and social collaboration capabilities designed to provide a superior user experience. EasyVista will also unveil new workflow automation capabilities that allow for easy interactions with 750+ software solutions. Freshservice (Booth 318) will announce the launch of project management, machine learning-driven self-service, advanced analytics, and a refreshed mobile app. By enabling deeper collaboration between agents and giving them the required context to provide great service to end-users, this proactive approach to service management will improve service quality and reduce agent workload. ISL Online (Booth 526) will showcase helpdesk tools that raise productivity and allow users to resolve issues efficiently. The customizable, multi-platform software provides unattended access and the ability to remotely troubleshoot mobile devices. ISL integrates with tools from ticketing software to CRMs and options include Saas, Self-Hosted or Private Cloud. Ivanti (Booth 303) brings critical ITSM data in sync through the addition of Xtraction reporting and dashboard connectors for Ivanti Service Manager, Voice and additional third party applications. With these latest developments, Xtraction delivers richer data insights on demand, helping users visualize key information such as call abandon rates, coupled with financial impacts. Lionbridge (Booth 326) will showcase its new solution, GeoFluent for Enterprise Service Management, which enables organizations to provide multilingual helpdesk and service support without hiring bilingual staff. By eliminating language barriers across communications channels, from chat and ticketing to voice, productivity and efficiency are improved and speed of response and resolution is increased. Samanage (Booth 309), will demonstrate the latest in service desk intelligence. Each hour at the Astro Bar within the Samanage Booth, the team will highlight how to create customer automations, establish multi-level approvals, and utilize the enhanced integrations found within Samanage, the smartest service desk. ServiceAide (Booth 428) will reveal its unique architecture to create and manage multi-level SaaS environments enhanced with machine intelligence, taking Service Management efficiency to new levels. Cloud Service Management™ will be showcased, featuring its ITIL-based processes, friction-free onboarding, simplicity of use, and low administrative burden and cost of ownership making it highly affordable. SUMMIT Software Inc. (Booth 229), a leading provider of private and public cloud based IT operations management solutions, will demonstrate Productivity 360, a productivity engine built into its flagship solution, SUMMIT ITOM Platform. The versatile engines provide IT analysts with operational intelligence, gamification, service automation, service assist and skill management. Stefanini (Booth 606), one of the most important global providers of IT outsourcing, applications management, consulting and strategic staffing services, will showcase its portfolio of innovative IT outsourcing practice solutions. These solutions help supplement clients' current help desk operations and assist with migrating the installed base of desktops. StrataCom (Booth 329) will highlight its IT service management professional services and consulting expertise for enterprise-level clients. The services offer a better, faster, more innovative and affordable ITSM solution that fulfills IT help desk needs, as well as the needs of other departments within the organization SunView Software (Booth 414) will showcase its new capabilities to improve end-user experience. These innovative features include a mobile application that gives users an easier and more convenient way to access the ChangeGear platform; and Willow, a Chatbot virtual support assistant powered by artificial intelligence and the award-winning Service Smart Technology. SysAid (Booth 618) will be showcasing the latest in self-service within its help desk and ITSM solution – which will include finger authentication capabilities for the end users. Demonstrations will also be hosted at the booth and will address SysAid's growing marketplace offering pre-built integrations with leading software applications and services. TEKsystems Global Services (Booth 315) will discuss how to effectively manage the complexities of service management transformation as technology continues to evolve at an exponential pace. At the booth, their dedicated team will provide insight into IT service management, service desk, desktop support and engineering, and technology deployment solutions. TOPdesk (Booth 321) will announce its partnership with OneLogin to provide single sign-on, and multi-factor authentication for TOPdesk's clients. This partnership formalizes a joint commitment to providing secure experiences for users and administrators. TOPdesk will also demonstrate its integration with RES Software that helps resolve service times, reduce cost, improve security and deliver predictable service. About HDI Founded in 1989, HDI is the first membership association and certification body created for the technical support industry. Since then, HDI has remained the source for professional development by offering the resources needed to promote organization-wide success through exceptional customer service. In other words, we help professionals in service management better serve customers. We do this by facilitating collaboration and networking, hosting acclaimed conferences and events, producing renowned publications and research, certifying and training thousands of professionals each year, and connecting solution providers with practitioners. Learn more at www.ThinkHDI.com. HDI is organized by UBM plc. UBM is the largest pure-play B2B Events organizer in the world. Our 3,750+ people, based in more than 20 countries, serve more than 50 different sectors. Our deep knowledge and passion for these sectors allow us to create valuable experiences which enable our customers to succeed. Please visit www.ubm.com for the latest news and information about UBM. To view the original version on PR Newswire, visit:http://www.prnewswire.com/news-releases/more-than-20-vendors-announce-new-products-and-services-at-hdi-2017-300448362.html
News Article | May 1, 2017
« DOE: 58% of BEV sales in US in 2016 were large cars and standard SUVs; PHEVS mainly compact and midsize | Main | Propel Fuels promoting E85 in California with “105 Octane Tour”; $1.05/gallon » Cummins Westport (CWI) announced its model year 2018 dedicated natural gas engines for regional haul truck / tractor, vocational and transit, school bus, and refuse applications at the Alternative Clean Transportation (ACT) Expo in Long Beach, California. The new lineup comes with a change in names, following Cummins tradition of using B, L, and X series letters, followed by engine displacement. The letter “N” denotes engines that are fueled by natural gas. The new B6.7N, L9N, and ISX12N engines feature Environmental Protection Agency (EPA) and California Air Resources Board (ARB) Optional Low NO certification, On-Board Diagnostics (OBD), Closed Crankcase Ventilation (CCV) systems, and performance and reliability improvements. (Earlier post.) The new ISX12N features a redesigned fuel system with fewer parts and improved performance. Our 2018 product line demonstrates an important milestone in product development for Cummins Westport, creating a move to zero emissions strategy for our customers and industry. We are particularly pleased that the ISX12N will join the L9N in offering our on-highway customers the benefits of performance and reliability at an ultra-low emissions level described by California’s South Coast Air Quality Management District as equivalent to an electric vehicle. This move to zero emissions strategy means our customers can choose the most affordable path to zero-equivalent emissions with no commercial constraints on supply or technology readiness. Like the L9N that replaces the ISL G Near Zero, the 2018 ISX12N heavy-duty natural gas engine for regional haul truck / tractor, vocational, and refuse applications will also be certified to EPA and California ARB optional low NO Emissions standards of 0.02 g/bhp-hr. The new ISX12N and L9N will be the lowest certified NO emission engines available in North America. NO exhaust emissions are 90% lower than the current EPA NOx limit of 0.2 g/bhp-hr, and the engines also meet or exceed the 2017 EPA greenhouse gas (GHG) emission requirements. CWI natural gas engines have met the 2010 EPA standard for particulate matter (0.01 g/bhp-hr) since 2001. All CWI engines offer customers the choice of using compressed natural gas (CNG), liquefied natural gas (LNG) or renewable natural gas (RNG) as a fuel. Using low carbon intensity RNG fuel provides significant well-to-wheel GHG reductions and is an important aspect of a move to zero emissions strategy. According to California ARB Low Carbon Fuel Standard (LCFS) studies, RNG can reach subzero GHG carbon intensity levels. Cummins Westport engines utilize proprietary spark-ignited, stoichiometric combustion with cooled exhaust gas recirculation technology, and three-way catalyst aftertreatment (TWC). The TWC is packaged as a muffler and is maintenance-free. No diesel particulate filter or selective catalytic reduction aftertreatment is required. CWI 6.7 to 12 liter engines are designed for truck and bus applications up to 80,000 pounds. Available from leading truck and bus OEMs, vehicles can be tailored to perform to meet customer requirements with enough range to offer route flexibility without in-route refueling. For example, on highway natural gas trucks can have over 700 mile range capability. The ISX12N will be manufactured in Cummins’ heavy-duty engine plant in Jamestown, New York. The L9N and the B6.7N are manufactured in Cummins midrange engine plant in Rocky Mount, North Carolina. Partial funding in support of the ISX12N engine development has been received from South Coast Air Quality Management District, the California Energy Commission, Southern California Gas and Clean Energy.
News Article | May 3, 2017
« Constellium providing lightweight aluminium solutions for new BMW 5 Series | Main | US Hybrid unveils new Class 8 fuel cell port drayage truck for San Pedro Ports » Kenworth continues its advancements on low/zero emission projects focused on Kenworth T680 day cabs for drayage tractor operation in Southern California ports, which are backed by $9 million in government grants awarded last August. Kenworth is developing a prototype Class 8 hydrogen fuel cell tractor, using the Ballard Power Systems fuel cell to recharge the battery pack. The hydrogen fuel cell series hybrid T680 day cab tractor uses lithium-ion batteries to power a dual-rotor electric motor, driving the rear tandem axle through a 4-speed automated transmission. Kenworth’s hydrogen truck is expected to be ready for initial track and on-road testing in the fourth quarter of this year. Kenworth has also begun building a second prototype series hybrid-electric T680 day cab designed to produce near-zero-emissions. The truck will use the currently available Cummins Westport ISL G Near Zero NOx engine fueled by compressed natural gas (CNG) to generate electrical power. Initial track and road testing is expected to start in the fourth quarter. These two T680 tractors will be identical, with the exception of their power generation systems. Each truck will have an electric-only range of approximately 30 miles (48 km), and the on-board fuel—hydrogen or natural gas—will provide sufficient range for a full day in regional haul applications. Kenworth’s work on these programs is supported by grants of $2.1 million for each project from the U.S. Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy (EERE), with Southern California’s South Coast Air Quality Management District (SCAQMD) as the prime applicant. Kenworth T680 Hybrid-Electric Tractor with CNG. A third project received $4.8 million in funding from the California Air Resources Board (CARB), again with the SCAQMD as the prime applicant. Kenworth will build four additional, hybrid-electric T680 day cabs equipped with the Cummins Westport ISL G Near Zero NOx engine operating on compressed natural gas, and will also support customer field tests of these units in Southern California drayage operations. Kenworth will build its first unit for this project in 2018. Kenworth engineers will be able to make design and system refinements to this 2018 unit based on data collected from this year’s real-world testing of the initial, 2017 hybrid-electric T680. All six prototype T680 day cab drayage tractors, produced as a result of these Kenworth programs, will transport freight from the Ports of Los Angeles and Long Beach to warehouses and railyards in the Los Angeles basin.
News Article | February 15, 2017
The spliceosome converts from branching to exon ligation through the ATP-dependent activity of Prp16, a DEAH box helicase8. Prp16 action destabilizes the branching factors Cwc25 and Yju2 (refs 4,9) and creates strong binding sites for the exon ligation factors Slu7 and Prp18 (refs 10,11), while promoting 3′ exon docking7. These factors are essential for splicing of pre-mRNAs with long distances between the branch point and the 3′ splice site12 and for correct 3′ splice site selection13, but their precise roles are unknown. To understand the mechanism of Prp16-mediated spliceosomal remodelling, we assembled spliceosomes in S. cerevisiae extracts on a pre-mRNA substrate containing a deoxy-guanosine at the 3′ splice site UAG sequence and purified them via an affinity-tag on Slu7 (Methods). With this substrate the spliceosomes stall after Prp16-dependent remodelling and before exon ligation, forming the C* complex14; thus purified spliceosomes contain predominantly lariat intermediates (Extended Data Fig. 1). We obtained a cryoEM reconstruction of C* complex at a resolution of 3.8 Å (Extended Data Figs 1, 2), into which we modelled 40 components (Extended Data Tables 1, 2, Extended Data Figs 3, 4 and Supplementary Information). Prp8, Snu114 and the U5 Sm core domain form the foot domain in both C* and C complexes (Fig. 1), which functions as an assembly platform for most of the Prp19 complex (NTC) and Prp19-related (NTR) components including Cwc2, Bud31 and Ecm2. The N-terminal end of Clf1 is anchored by Cef1, Syf2 and the U2/U6 snRNAs exiting the active site (Fig. 1b). The HAT repeats of Clf1 and Syf1 together form a large arch which is rotated considerably in C* relative to the C complex. The N-terminal end of Syf1 interacts with the U2 small nuclear ribonucleoprotein (snRNP), therefore this rotation disrupts the interface between the Prp8 RNase H-like (RH) domain and the U2 snRNP observed in C complex. Consequently, the Prp8 RH domain rotates inward (Extended Data Fig. 5) and the Prp17 WD40 domain moves into the body of the complex. U2 stem IIc swings outwards and no longer interacts with Cwc2/Ecm2 (Extended Data Fig. 5). Unlike in the C complex, no density is visible for Brr2. The RNA structure in the core of the spliceosome remains remarkably unchanged during the C to C* transition, with the exception of the branch helix (Fig. 2a, b). The U2/U6 catalytic triplex adopts the same configuration as in the C complex, consistent with biochemical and genetic evidence15. Density consistent with the presence of Mg2+ ions is observed adjacent to the phosphate oxygen ligands for catalytic metal ions M1 and M2 identified by metal rescue studies (Extended Data Fig. 6), providing further evidence for a single active site for both catalytic steps2. As in the C complex, the 5′ exon is base-paired with loop 1 of U5 snRNA in agreement with genetic analysis and crosslinking experiments16, 17, and the 3′-OH of the last 5′-exon nucleotide (G(−1)) lies close to the M1 site and is ready to act as a nucleophile for the incoming 3′ splice site (Fig. 2c, Extended Data Fig. 6). Prp16-induced remodelling results in a dramatic rotation of the branch helix by approximately 75° around the hinge at A30 of U2 snRNA (Fig. 2b). The branch point moves away from the catalytic centre (approximately 20 Å), creating sufficient space for the 3′ splice site to dock at the catalytic Mg2+ site (Fig. 2a, c). This movement disrupts the non-Watson–Crick interactions of the branch point adenosine (A70) with the branch helix4 and reorganizes the interactions of the 5′ splice site with the U6 snRNA ACAGAGA sequence (Fig. 2d, e). The A70 base is packed against the ribose of the first intron nucleotide G(+1), while the G(+1) base stacks with the base of U(+2). In the C complex, U(+2) forms a base triple with G37 of U2 snRNA and C67 of the intron4, 5 whereas in the C* complex U(+2) forms a non-canonical base-pair with A51 of U6 snRNA (Fig. 2d, e), consistent with crosslinking in human spliceosomes17. Notably, mutations at both U(+2) and A51 impair exon ligation18, 19. Indeed, in group II introns the nucleotide equivalent to A51 (adjacent to the two nucleotides involved in triplex formation) base-pairs with the last nucleotide of the intron (γ–γ′ interaction)20. Thus A51 and intron U(+2) may interact with the last nucleotide G(−1) of the intron. It is noteworthy that all three positions (A70, G(+1), U(+2)/A51), whose mutations lead to second step defects, are aligned towards the active site, strongly suggesting a path for the 3′ splice site (Fig. 2c). Finally, the Hoogsteen base-pair between A(+3) of the intron and G50 of U6 snRNA (Fig. 2e) no longer forms in C* (Fig. 2d), consistent with genetic evidence that this interaction must be disrupted during the Prp16 rearrangement21. The proteins common to both C and C* restrain the catalytic RNA core (U6 snRNA ISL and helices Ia and Ib) onto Prp8, whereas the branch helix rotates substantially between the two states (Fig. 2b). In the C complex, the branch helix is locked into the branching conformation predominantly by Cwc25, Yju2 and Isy1, such that A70 is inserted into the catalytic centre4, 5 (Fig. 3a, b). After branching, Prp16 promotes dissociation of Cwc25, Yju2 and Isy1 and binding of Prp18, Slu7 and Prp22. In the C complex the U2 Sm core domain interacts with the RH domain4, 5 but this interaction is disrupted completely in the C* complex when Prp17 wedges between the U2 Sm ring and the Prp8 RH domain (Fig. 3a, Extended Data Fig. 5). In the C* complex, the RH domain of Prp8 is rotated by about 80° with respect to the large domain (Extended Data Fig. 5d, e). In this orientation, the β-finger of the RH domain crosses the minor groove of the branch helix and reaches Cef1 (Fig. 3a, d). Prp17 binds across the interface between the β-finger and Cef1, stabilizing this interaction. A long α-helix bridges the Prp8 RH domain and the Cef1 Myb domain and reaches the C terminus of Syf1 (Fig. 3c). The direction and sequence of this helix are uncertain from the current map. These interactions lock the branch helix in a conformation predisposed for exon ligation. Prp17 and the rotated position of the RH domain observed in C* would clash with Isy1 and Cwc25, explaining how Prp16-dependent dissociation of Isy1 enables the RH domain to rotate, promoting the exon-ligation configuration. Consistently, deletion of Isy1 suppresses a Prp16 mutation that impairs remodelling of the spliceosome22. The helical domain23 of Prp18 is bound to the Prp8 RH domain opposite from the branch helix binding face (Fig. 3a). Slu7 meanders from the binding site of its predicted globular region towards the foot of the complex, interacts with Prp18, and latches the RH domain onto the endonuclease domain of Prp8, thus stabilizing the rotated conformation of the RH domain and the binding of Prp18 in C* (Extended Data Fig. 7). Indeed, the region of Slu7 that binds the RH domain is essential for yeast viability24. Our C* complex structure provides important insight into the organization of the active site during exon ligation even though the 3′ exon is not yet docked. As discussed above the rotation of the branch helix not only creates a space for the 3′ exon at the catalytic metal binding site but also reorganizes the interaction between the U6 snRNA ACAGAGA sequence and the 5′ end of the intron. An important outcome of Prp16 action is repositioning A51 of U6 snRNA so that it could position the 3′ splice site by interacting with the last nucleotide of the intron like the equivalent nucleotide in group II introns20 (Fig. 2c). If this is the case, it is possible that the penultimate nucleotide A(−2) and its preceding nucleotide Y(−3) may also interact with the first intron nucleotide G(+1) and the branch point adenosine (A70). An interaction between the 3′ splice site UAG sequence, the ACAGAGA sequence and the 5′ splice site intron sequence for exon-ligation has been suggested previously19. However, mutational studies of the 5′ splice site and 3′ splice site have not led to a clear base-pairing scheme19, 25 suggesting that these interactions may involve non-canonical base pairing. The base pairing between the 5′ exon and loop 1 of U5 snRNA (Fig. 2c) places the 3′-OH group of the 5′ exon close to M1 such that it can act as a nucleophile when the phosphate group at the 3′ splice site is bound to M1 and M2 (Extended Data Fig. 6; refs 2,26). Slu7 and Prp18 are dispensable for exon ligation when the distance between the branch point and the 3′ splice site is less than nine nucleotides11. Notably, in our structure, three nucleotides of the intron downstream of the branch point are visible and an additional six nucleotides would be sufficient to fold back and reach the catalytic Mg2+ site (Extended Data Fig. 8; ref. 12). When the distance to the 3′ splice site is less than nine nucleotides, the 3′ splice site could easily reach the catalytic centre and allow the 3′ exon to dock. When the distance is much greater, the entropic cost of docking the 3′ exon would be greater and Slu7 and Prp18 could become indispensable in guiding the path of the intron (Extended Data Fig. 8). Indeed, mutation of Slu7 impairs splicing at distal 3′ splice sites without affecting proximal 3′ splice sites, when two competing sites are present13, 27. The DEAH ATPase Prp22, which promotes exon ligation7, 28, binds on top of the Prp8 large domain (Fig. 4a) near where Prp16 binds in the C complex, consistent with a mutually exclusive interaction with the spliceosome10. Compared to the crystal structure of the homologous Prp43 ATPase in the ADP–Mg-bound form29, Prp22 in our C* complex is in an open conformation with a wider separation of the RecA1 and RecA2 domains (Fig. 4b). Density attributable to RNA is present in the Prp22 active site (Fig. 4b) and Prp22 crosslinks 17 nucleotides downstream of the exon–exon junction6. Indeed, the distance between the catalytic centre of the spliceosome and Prp22 in our structure can be spanned with 16–17 nucleotides. Thus Prp22 could bind to the 3′ exon emerging from the core to promote mRNA release and dissociation of Slu7, Prp18 and Cwc22 after exon ligation. Our C* complex structure elucidates the structural consequences of Prp16 activity (Fig. 4c), reveals a large rotation of the branch helix which creates a space for 3′ exon docking at the catalytic centre in the exon ligation conformation of the spliceosome, and provides a structural framework for investigating 3′ splice site selection and ligated exon release.
News Article | February 15, 2017
The Wise Marketer Group, which provides loyalty marketing news, research and education to marketing professionals worldwide, announced today a sponsorship and content marketing agreement with Reward Paths, a customer loyalty solutions enterprise serving the U.S., Canadian and Caribbean rewards, loyalty and incentive solutions marketplace. Reward Paths and its parent company, Incentive Solutions Ltd (ISL), Auckland, New Zealand, designs, operates and technically enables loyalty and incentive programs in both the consumer and B2B marketing arenas. The combined entities currently operate in the US, Canada, Jamaica, New Zealand and Australia markets. The marketing agreement will enable both Reward Paths and ISL to become a primary sponsor of the B2B loyalty “channel” at The Wise Marketer and deliver loyalty marketing educational courses through The Loyalty Academy. Reward Paths offers clients proven program design, operations and rewards expertise, enabled by affordable, best-in-class technology that has been previously unavailable to mid-market clients, especially in the B2B sector. According to David Harwood, CEO of Reward Paths, the partnership will enhance collaboration with marketing executives from companies and brands worldwide on research, intelligence and best practices to improve the results of B2B loyalty programs. “At Reward Paths and ISL, we intend to play a lead role in creating and shaping new directions that B2B loyalty programs and strategies will be taking in the next few years, change that is demanded by swift moving market, technology and cultural forces. The new Wise Marketer's focus on re-invigorating loyalty thought leadership and practitioner excellence makes them a natural partner for us in that effort," Harwood said. "We're excited to see the Wise Marketer bring a focus that has been missing from the market in terms of developing loyalty expertise as a professional goal for new marketers. Beyond the value of the Wise Marketer as a go-to loyalty publication, we strongly support the value of their newly launched Loyalty Academy as a training source and annual event to help grow the next generation of loyalty professionals," he said. Wise Marketer Group Chief Executive and Editor-in-Chief Rick Ferguson is leading the creation of a forward-looking vision for the group and stated, “The brands that sponsor loyalty programs and the organizations providing thought leadership, technology and creative support to enable program success share common interests. We are proud to announce Reward Paths as a founding sponsor and look forward to working together to develop an industry resource that every executive in this industry will consider a must-have tool to stay abreast of this dynamic industry." Added Harwood, “We have significant experience in New Zealand and Australia about what works and why, especially in B2B and consumer coalition programs. We have engineered our North American business around similar concepts. While some cultural and market adjustments are always required, the reward and recognition foundation we have established means that all mid-market industries with any interest in a loyalty or rewards solution will benefit greatly.” About Reward Paths, LLC Reward Paths is a full service Marketing firm specializing in helping mid-market companies design, enable and operate reward, loyalty and incentive programs for their customers and associates. Reward Paths offers affordable, best of breed technology especially well suited to the business-to-business marketplace and operates the world’s first web resource devoted to B2B loyalty information at http://www.b2bloyaltyguide.com/. The company also provides reward program services to mid-market clients and coalitions serving consumer markets. Reward Paths LLC is majority owned by a subsidiary of Incentive Solutions Limited (ISL) of Auckland, New Zealand. For more information visit [http://www.rewardpaths.com. About The Wise Marketer Group The Wise Marketer Group delivers timely and unbiased publishing, research, and educational products to a global audience of marketing professionals. In addition to publishing the Wise Marketer, the Wise Marketer Group also operates the Loyalty Academy (http://www.loyaltyacademy.org), the premiere global education and membership organization for loyalty marketing practitioners, and publishes the Loyalty Guide (http://www.loyaltyguide.com), now in its 7th edition, which offers over 1,400 pages of unrivaled customer loyalty and marketing intelligence for marketing leaders. The Wise Marketer also hosts the annual Loyalty Academy Conference, scheduled for March 2, 2017 at the Marriott Harbor Beach Resort and Spa in Ft. Lauderdale, FL.
News Article | February 24, 2017
VANCOUVER, BRITISH COLUMBIA--(Marketwired - Feb. 24, 2017) - Copper Fox Metals Inc. ("Copper Fox" or the "Company") (TSX VENTURE:CUU)(OTC PINK:CPFXF) is pleased to announce that its audited consolidated October 31, 2016 financial statements have been filed on SEDAR. All of the Company's material subsidiaries are wholly owned, except for Carmax Mining Corp. ("Carmax") (TSX VENTURE:CXM), of which the Company owns 65.4% of the outstanding common shares. These audited consolidated financial statements include 100% of the assets and liabilities related to Carmax and include a non-controlling interest portion, representing 34.6% of Carmax's assets and liabilities that are not owned by the Company. For the year ended October 31, 2016, Copper Fox had a comprehensive loss of $784,363 (October 31, 2015 - $20,215) which equated to $0.00 loss per share (October 31, 2015 - $0.00). During the fiscal year ended, the Company incurred $477,945 in expenditures toward furthering the development of its Van Dyke, Sombrero Butte, and Mineral Mountain copper projects in Arizona. Copies of the financial statements, notes, and related management discussion and analysis may be obtained on SEDAR at www.sedar.com, the Company's web site at www.copperfoxmetals.com or by contacting the Company directly. All references to planned activities and technical information contained in this news release have been previously announced by way of news releases. All amounts are expressed in Canadian dollars unless otherwise stated. Elmer B. Stewart, President and CEO of Copper Fox stated, "In 2016, Copper Fox achieved positive technical results on all five of its projects, while considerably reducing its general and administrative expenses. At Schaft Creek, the joint venture completed a geological model and commenced updating the Schaft Creek deposit resource model, in addition to other project related activities. The Preliminary Economic Assessment ("PEA") of the Van Dyke project indicated that the anticipated costs to produce a pound of copper is in the first quartile for global copper costs, identified a number of economic enhancements, and recommended the completion of a pre-feasibility study. The Mineral Mountain, Sombrero Butte, and Eaglehead (Carmax) projects were all advanced technically during the fiscal year ended 2016, and all yielded positive results. One potential issue that arose during the fiscal year ended 2016 was Carmax's ownership of its Eaglehead mineral tenures. This issue has been heard by the courts and the Company is now awaiting a decision. Until said decision is rendered, Carmax's ownership of the Eaglehead mineral claim tenures remains in doubt. Going into 2017, Copper Fox will continue its strategy adopted in 2016, which is to focus on advancing its projects through exploration and development." Elmer B. Stewart, MSc. P. Geol., President of Copper Fox, is the Company's non-independent, nominated Qualified Person pursuant to National Instrument 43-101, Standards for Disclosure for Mineral Projects, and has reviewed and approves the scientific and technical information disclosed in this news release. As at October 31, 2016, the Company had $847,505 in cash (October 31, 2015 - $1,529,138). Copper Fox is a Tier 1 Canadian resource company listed on the TSX Venture Exchange (TSX VENTURE:CUU) focused on copper exploration and development in Canada and the United States. Copper Fox and its wholly owned Canadian and United States subsidiaries, being Desert Fox Copper Inc. and Northern Fox Copper Inc., hold the five primary assets listed below: On behalf of the Board of Directors, Neither TSX Venture Exchange nor its Regulation Services Provider (as that term is defined in the policies of the TSX Venture Exchange) accepts responsibility for the adequacy or accuracy of this release. This news release contains forward-looking statements within the meaning of the Section 27A of the Securities Act of 1933 and Section 21E of the Securities Exchange Act of 1934, and forward-looking information within the meaning of the Canadian securities laws (collectively, "forward-looking information"). Forward-looking information in this news release include statements about the anticipated costs to produce a pound of copper at the Van Dyke project; focusing on advancing the Company's projects through exploration and development; a $0.9 million budget for the 2017 Schaft Creek Joint Venture program, including completion of the resource remodeling, desktop engineering and trade-off studies, collection of baseline environmental data, and an application for a MYAB permit; approval of the MYAB permit and the underlying activities; projections for the Van Dyke In-Situ Leach ("ISL") copper project to have low cash costs (first quartile) per pound of copper produced, strong cash flows and a pre-tax Net Present Value ("NPV") of US $213.1 million, coupled with an Internal Rate of Return ("IRR") of 35.5%; and a cost of approximately US $425,000 to obtain the main permits for the ISL test. In connection with the forward-looking information contained in this news release, Copper Fox and its subsidiaries have made numerous assumptions regarding, among other things: the geological, financial and economic advice that Copper Fox has received is reliable and is based upon practices and methodologies which are consistent with industry standards; and the stability of economic and market conditions. While Copper Fox considers these assumptions to be reasonable, these assumptions are inherently subject to significant uncertainties and contingencies. Additionally, there are known and unknown risk factors which could cause Copper Fox's actual results, performance or achievements to be materially different from any future results, performance or achievements expressed or implied by the forward-looking information contained herein. Known risk factors include, among others: exploration of the projects may not find copper mineralization in significant quantities or at all, or at the expected cost; the overall economy may deteriorate; uncertainty as to the availability and terms of future financing; copper prices and demand may fluctuate; currency exchange rates may fluctuate; conditions in the financial markets may deteriorate; and uncertainty as to timely availability of permits and other governmental approvals. A more complete discussion of the risks and uncertainties facing Copper Fox is disclosed in Copper Fox's continuous disclosure filings with Canadian securities regulatory authorities at www.sedar.com. All forward-looking information herein is qualified in its entirety by this cautionary statement, and Copper Fox disclaims any obligation to revise or update any such forward-looking information or to publicly announce the result of any revisions to any of the forward-looking information contained herein to reflect future results, events or developments, except as required by law.