Ishikawa Agriculture and Forestry Research Center

Kanazawa-shi, Japan

Ishikawa Agriculture and Forestry Research Center

Kanazawa-shi, Japan
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Gao-Takai M.,Ishikawa Prefectural University | Katayama-Ikegami A.,Ishikawa Prefectural University | Nakano S.,Ishikawa Agriculture and Forestry Research Center | Matsuda K.,Ishikawa Agriculture and Forestry Research Center | Motosugi H.,Kyoto Prefectural University
Horticulture Journal | Year: 2017

The growth and berry quality of ‘Ruby Roman’ (Vitis labruscana) grapevines grafted on two species of rootstock, ‘Kober 5BB’ [‘5BB(2×)’] (V. berlandieri × V. riparia, a semi-dwarf rootstock) and ‘Hybrid Franc’ [‘HF(2×)’] (V. rupestris × V. vinifera, a vigorous rootstock), and their colchicine-induced autotetraploids [‘5BB(4×)’ and ‘HF(4×)’] were investigated through 2 years of pot cultivation followed by 2 years of ground cultivation. During the nursery stage, the survival rate and root and shoot growth of the grafted cuttings in the two diploid rootstocks were obviously greater than in their corresponding tetraploids. During subsequent cultivation in pots and in the ground, the grapevines grafted on ‘5BB(4×)’ had less shoot growth (weight and length), shorter internode length, and in some cases smaller stem diameter than those grafted on ‘5BB(2×)’. However, in contrast with ‘5BB’, there was no significant difference in total vine growth between ‘HF(2×)’ and ‘HF(4×)’ during pot cultivation, and the total shoot length and weight in the ‘HF(4×)’ grapevine was greater than in ‘HF(2×)’ during ground cultivation in 2014. After 2 years of pot cultivation, the root growth of the ‘HF’ (diploid and tetraploid) rootstocks was more vigorous than that of the ‘5BB’ (diploid and tetraploid) rootstocks. In addition, the proportions of the thin roots (diameters <1 mm) in the two diploid rootstocks [‘5BB(2×)’ and ‘HF(2×)’] were greater than those in the two tetraploid rootstocks [‘5BB(4×)’ and ‘HF(4×)’]. In contrast, the proportions of the thick roots (diameters 1–2 mm, 2–5 mm, and >5 mm) in the two diploid rootstocks were less than those in the two tetraploid rootstocks. Furthermore, the berries of ‘Ruby Roman’ harvested from the grapevines grafted on ‘5BB(4×)’ exhibited a much deeper skin coloration than those harvested from the other grapevines. © 2017 The Japanese Society for Horticultural Science (JSHS), All rights reserved.


Miyawaki O.,Ishikawa Prefectural University | Omote C.,Ishikawa Prefectural University | Koyanagi T.,Ishikawa Prefectural University | Sasaki T.,Industrial Research Institute of Ishikawa | And 5 more authors.
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2017

Pineapple juice was concentrated from 13. 0 to 22.3 Brix by progressive freeze-concentration (PFC). The distributions of organic acids and flavors were measured and no substantial changes were observed in the component profiles before and after PFC concentration. The PFC-concentrated pineapple juice was fermented to produce pineapple wine with an ethanol concentration as high as 12.7 vol-%, indicating that a sufficient alcohol level can be obtained using PFC concentration without chaptalisation. After fermentation, acetic acid and succinic acid increased in the organic acid distribution. In terms of flavors, several of the esters in the original juice disappeared, while fermentation products, such as ethanol, 3-methyl-1-butanol, ethyl octanoate, ethyl decanoate, methyl 3-acetoxyhexanoate and benzene ethanol, appeared after fermentation. Generally, the pineapple wine retained enough amount of flavors present in the original juice. These findings suggest that a new-type pineapple wine, presently not available on the market, can be produced by PFC. Copyright © 2017, Japanese Society for Food Science and Technology.


Miyawaki O.,Ishikawa Prefectural University | Gunathilake M.,Sudan University of Science and Technology | Omote C.,Ishikawa Prefectural University | Koyanagi T.,Ishikawa Prefectural University | And 6 more authors.
Journal of Food Engineering | Year: 2016

Progressive freeze-concentration (PFC) by a tubular ice system was successfully applied to concentrate apple juice from 13.7 to 25.5 oBrix under a program controlled operation for the coolant temperature and the circulation pumping speed. The organic acid distribution and the flavor profile analysis revealed that no substantial differences were observed for the juice before and after concentration both in organic acids and flavor components showing the high quality concentration by PFC. This was also confirmed by electronic taste and flavor analyzers. The PFC-concentrated apple juice was fermented to obtain a new type apple wine with alcohol content as high as 13.7 vol-% without chaptalization. The organic acid distribution was slightly changed before and after fermentation while the flavor profile changed drastically. The present technique will be applicable to produce new type of wine from many other fruits. © 2015 Elsevier Ltd.


Morimoto E.,Ishikawa Agriculture and Forestry Research Center | Daikoku M.,Tohoku Agricultural Research Center Shimokuriya gawa Morioka | Ogomi T.,Isekiand Co. | Saiga M.,Isekiand Co. | Aoki K.,Isekiand Co.
Engineering in Agriculture, Environment and Food | Year: 2016

This study observed soil condition in a tsunami-damaged field. An on-the-go soil sensor mounted on rice transplanter was employed for field monitoring. Topsoil depth (TD) and soil fertility value (SFV) were used for field evaluation. A dataset of 17,017 samples for tsunami-damaged and 33,716 samples from on-damaged field were evaluated in this study. Results indicated that standard deviation of TD between tsunami and non-damaged fields showed very little differences. However, average SFV in the tsunami field was greater than three times higher than that of non-damaged field. Especially, high SFV (i.e. 1.2 mS/cm) was observed around the edge of field due to the salt removal operation by using mole drain. © 2016 Published by Elsevier B.V. on behalf of Asian Agricultural and Biological Engineering Association.


Miyawaki O.,Ishikawa Prefectural University | Omote C.,Ishikawa Prefectural University | Gunathilake M.,Sudan University of Science and Technology | Ishisaki K.,Ishikawa Agriculture and Forestry Research Center | And 3 more authors.
Journal of Food Engineering | Year: 2016

A 25L-scale tubular ice system for progressive freeze-concentration was constructed and combined with a 75L-scale partial ice-melting system for yield improvement. From the measurement of freezing point of various fruits juices, nine standard operation programs were prepared in cooling and circulation flow rate for the tubular ice system. The standard programs were successfully tested for progressive freeze-concentration of sucrose solutions with concentration varied from 3 to 30%. By choosing one of the standard program, apple juice was effectively concentrated from 12.8 to 21.0 °Brix with 79.0% yield, which was improved to 90% by recovering the 30% of the initially-melted fractions by using the partial ice-melting system. The tubular ice system can be easily scaled-up more, if necessary, simply by increasing the number of tubes. © 2016 Elsevier Ltd.


Miyashita N.,Ishikawa Prefectural University | Yabu T.,Ishikawa Agriculture and Forestry Research Center | Kurihara T.,Ishikawa Prefectural University | Koga H.,Ishikawa Prefectural University
Journal of Nematology | Year: 2014

Meloidogyne incognita is a parasitic root-knot nematode that causes considerable yield loss in a wide range of plants. In this study we documented the movement of adult female nematodes for more than 2 hr in micro-slices of infected tomato (Solanum lycopersicum) and rose balsam (Impatiens balsamina) plants using light and video microscopy. Stylet thrusting was followed by short pumping actions of the esophagus, dorsal esophageal gland ampulla, and metacorpal bulb. Regular thrusting was normally accompanied by head turning and always preceded continuous stylet thrusting aimed at a single point (for 20 to 90 sec). Females often held the stylet in a protruded position, while pulsating the metacorpus bulb, for about 30 sec. Subsequently, the stylet was paused in a retracted position for 5 to 40 sec. This sequence of behavior took 290 to 380 sec to complete. The procedure developed in this study provides a useful cytological technique to investigate the interaction between root-knot nematodes and the giant cells formed by infected plants. Scanning electron microscopy revealed that the head of the adult nematode was located in the narrow intercellular spaces among several giant cells. The anterior part of the head of the adult was folded like a concertina, whereas that of the secondstage juvenile was not. The labial disc and medial lips of second-stage juveniles seemed expanded and sturdy, whereas those of the adult were star-shaped, appeared to be contracted, and softer. These morphological differences in the heads of adult and secondstage juveniles are discussed with respect to their movement. © The Society of Nematologists 2014.


Uwagaki Y.,Ishikawa Agriculture and Forestry Research Center | Uwagaki Y.,Ishikawa Prefectural University | Matsuda E.,Ishikawa Agriculture and Forestry Research Center | Komaki M.,Ishikawa Agriculture and Forestry Research Center | And 4 more authors.
Plant Biotechnology | Year: 2015

A method for Agrobacterium-mediated transformation of Freesia×hybrida is described. Cormlet-derived calli of two cultivars, ‘Mosera’ and ‘Ishikawa f3’ were co-cultivated withAgrobacterium tumefaciens strain EHA105 harboring the binary vector pIG121-Hm, which included hygromycin phosphotransferase gene and an intron-containing β-glucuronidase gene in the T-DNA region. Callus pieces were co-cultivated with A. tumefaciens on the callus proliferation medium [Murashige and Skoog (MS) medium containing 1 mg l−1thidiazuron, 1 mg l−1 dicamba, 20 mg l−1 3′,5′-dimethoxy-4′-hydroxyacetophenone, 1% (w/v) glucose, 3% (w/v) sucrose, and 0.2% (w/v) Gelrite]. Then, they were cultured on the callus proliferation medium containing 300 mg l−1 cefatoxime and 10 mg l−1 hygromycin B. Hygromycin-resistant lines of both cultivars regenerated into plantlets after transfer onto MS medium containing 2 mg l−1 3-indoleacetic acid and 3 mg l−1 6-benzyl aminopurine and/or plant growth regulator-free MS medium. Transgenic plants were identified by β-glucuronidase assay and verified by Southern blot analysis. Two transgenic plant lines were obtained from 475 callus pieces of ‘Mosera’, and one transgenic plant line was obtained from 290 callus pieces of ‘Ishikawa f3’. This is the first report of the genetic transformation of Freesia. This method will allow the genetic improvement of this horticulturally important flower. © 2015 The Japanese Society for Plant Cell and Molecular Biology.


Kodani J.,Ishikawa Agriculture and Forestry Research Center | Ogura A.,Ishikawa Agriculture and Forestry Research Center
Nihon Ringakkai Shi/Journal of the Japanese Forestry Society | Year: 2014

We investigated the recovery of vegetation on the spur road established in several Cryptomeria japonica plantations from one to seven years after thinning. From GLM analysis, the year after establishment was considered the primary factor for the recovery of vegetation in most models. The scattering of branches had no effect. The mean number of species at the third year after establishment was 12. 4 per m2 and it was 10. 9 per m2 at the fifth to seventh year. The mean vegetation coverage was 58% at the third year and 86% at the fifth to seventh year. The number of species and vegetative coverage of herbaceous plants increased until the third year and decreased thereafter whereas these of woody plants increased by the fifth year and did not change thereafter. Meanwhile, vegetative coverage of Sasa palmate increased with the year. From these results, vegetation recovery of the spur road is considered to increase irrespective of the scattering of branches with a change of dominance from herbaceous to woody plants or bamboo grass at the third to fifth year after the establishment, and to contribute to the stabilization of road surface.

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