Ishikawa Agricultural Research Center

Ishikawa, Japan

Ishikawa Agricultural Research Center

Ishikawa, Japan

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Ikeshita Y.,Ishikawa Agricultural Research Center | Kanamori Y.,Ishikawa Agricultural Research Center | Fukuoka N.,Ishikawa Agricultural Research Center | Kano Y.,Ishikawa Prefectural University
Scientia Horticulturae | Year: 2010

To investigate the effects of night-time temperature on cell and fruit size, and sugar accumulation in watermelon fruit, fruits were treated with high night-time temperatures in a greenhouse. The minimum night-time ambient temperature of the heating box (18 °C) was approximately 6 °C higher than that of the control. The length, diameter and weight of heat-treated fruit at the end of heating treatment, 16 days after anthesis (DAA), were significantly greater than that of control fruit, but those at harvesting, 42 DAA, were almost the same in both treatments. Mean cell size of the outer portion of heat-treated fruit at 16 DAA was significantly larger than that of the control. Cell size of the fruits at 42 DAA did not differ between heat-treated and control fruits. Sucrose, glucose and fructose content of fruit at 16 DAA did not differ between heat-treated and control fruit. However, sucrose content of the outer portion of heat-treated fruit was 162% of that of control fruit at 42 DAA. Glucose and fructose contents at 42 DAA did not differ between heat-treated and control fruit, except glucose content of outer portion. © 2010 Elsevier B.V.


Morimoto E.,Ishikawa Agricultural Research Center | Hirako S.,Shibuya Machinery Co. | Yamasaki H.,Iseki Co. | Izumi M.,Iseki Co.
Engineering in Agriculture, Environment and Food | Year: 2013

The objective of this paper was to provide the development of on-the-go soil sensor for rice transplanter, particularly from a perspective of precision agriculture applications. Ultrasonic sensor, electrodes and platinum resistance thermometer were employed for topsoil depth (TD) and apparent electrical conductivity (ECa) measurement. Soil fertility value (SFV) defined as new soil parameter, which consisted of ECa / TD. The results of field test revealed that the developed equipments could measure the TD (R 2 = 0.999), and the SFV had a strong relationship with measured EC (R2 = 0.937).


Maruyama T.,Ishikawa Prefectural University | Noto F.,Ishikawa Prefectural University | Murashima K.,Ishikawa Prefectural University | Hashimoto I.,Ishikawa Prefectural University | Kitada K.,Ishikawa Agricultural Research Center
Paddy and Water Environment | Year: 2010

A nitrogen balance study was carried out by setting up a test paddy in order to estimate the nitrogen pollution load potential (NPLP) from farmland in the Tedori River Alluvial Fan Areas and the load from the entire area under consideration was estimated using the cropping record and fertilizer application rate (FAR). The total NPLP was estimated to be 261 tons/year and the load from the paddy 79 tons/year for 5,704 ha, which would translate to an intensity of 14 kg/ha, while the load from the vegetable field was estimated to be 118 tons/year for 215 ha, which would equate to an intensity of 549 kg/ha. The pollution loads for the vegetables were significantly greater than those for the rice. The load for the barley was 57 tons/year for 261 ha (216 kg/ha) and that for the orchards was 23 tons/year for 64 ha (359 kg/ha). The estimation of soybean load was a negative 15 tons/year for 717 ha (-21 kg/ha), which meant that the nitrogen in the yield was greater than the FAR. The results also confirmed the yield absorption ratio in relation to the FAR. The load from the paddy for the entire area was also estimated using the percolation rate and the water quality load underneath the farmland was estimated to be 89 tons/year. © 2010 Springer-Verlag.


Kano Y.,Ishikawa Prefectural University | Ikeshita Y.,Ishikawa Agricultural Research Center | Kanamori Y.,Ishikawa Agricultural Research Center | Fukuoka N.,Ishikawa Agricultural Research Center
Acta Horticulturae | Year: 2013

To investigate the effects of night temperature on sugar accumulation in watermelon fruit, fruits were treated with higher night-time temperature in a greenhouse. The minimum night-time ambient temperature of the heating box (18°C) was approximately 6°C higher than that of the control. The weight of heat-treated fruit at the end of heating treatment, 16 days after anthesis (DAA), was greater than that of control, but fruit weight at harvest, 42 DAA, was almost the same in both treatments. Cells of all portions of the heat-treated fruit at 16 DAA were much larger than those of the control, and cells in the outermost rectangular parallelepipeds (RPs; 15-mm longsamples that were serially collected from a 10-mm thick disk along a 10-mm wide strip removed at the maximum transverse diameter of the fruit) of the heat-treated fruit were 80 μm or more larger than those of the control. However, cells from the outer portions of the heat-treated fruit at 42 DAA were smaller than those of the control. At 16 DAA, the number of RPs with sucrose concentration of 2 g L-1 or greater were 6 and 9 in control and heat treated fruit, respectively. At 42 DAA, the sucrose concentration in the outer RPs of the heat-treated fruit was much greater than that in the outer RPs of the control - the number of RPs with a sucrose concentration of 40 g L-1 or more was 5 in the control, and 11 in heat-treated fruit. Mean sucrose, glucose and fructose concentration in fruit at 16 DAA did not differ between treated and control fruit. However, at 42 DAA the sucrose concentration of heat-treated fruit was 32% greater than that of the control. Glucose and fructose concentrations were lower in heat-treated fruit than in the control. © 2013 ISHS.


Fukuoka N.,Ishikawa Prefectural University | Suzuki T.,Ishikawa Prefectural University | Yamada Y.,Ishikawa Agricultural Research Center
Journal of Horticultural Science and Biotechnology | Year: 2012

The aim of the present study was to determine the effects of infrared irradiation (IRI) on polyphenol biosynthesis and anti-oxidant activity in Gynura bicolor DC. leaves. To achieve this, infrared light lamps were placed 75 cm above the top of the canopy, and the plants were covered with a black nylon mesh to exclude the influence of natural light. Higher activities of ascorbate peroxidase (APX), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PDH), L-phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO) were detected in leaves when plants were subjected to IRI. Although IRI resulted in a significant decrease in anthocyanin content, HPLC analysis of the phenolic components showed a higher content of chlorogenic acid that was accompanied by a higher 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) radical-scavenging activity in IRI-treated leaves. These observations suggest that IRI causes oxidative stress and stimulates the activities of enzymes that regulate non-flavonoid polyphenol biosynthesis in G. bicolor leaves.


Sakamoto T.,Ishikawa Prefectural University | Masuda D.,Ishikawa Agricultural Research Center | Nishimura K.,Ishikawa Agricultural Research Center | Ikeshita Y.,Ishikawa Agricultural Research Center
Journal of Horticultural Science and Biotechnology | Year: 2014

Low sweetness in freshly-harvested tuberous roots is an undesirable characteristic of the sweet potato (Ipomoea batatas L. Lam.) cultivar, 'Kokei 14'. In the present study, we sought to characterise the biochemical and molecular aspects of sucrose accumulation in the tuberous roots of 'Kokei 14' during cold storage in comparison with another sweet potato cultivar, 'Beniazuma'.The tuberous roots of both cultivars were harvested and stored at 4°C for 30 d.The sucrose, fructose, and glucose concentrations, the activities of two sugar-metabolising enzymes, and the expression of six sucrose-cleaving acid invertase (AI) genes were monitored. Exposure to chilling temperatures (4°C) for up to 30 d increased the sucrose concentration in the tuberous roots of 'Kokei 14', but not in those of 'Beniazuma'. In line with this phenomenon, we found that the activity of AI was suppressed to approx. 0.4-times the pre-treatment value in 'Kokei 14', but increased to 2.7-times the pre-treatment value in 'Beniazuma' during cold storage for 30 d.The patterns and levels of expression of six genes encoding AIs indicated that the protein encoded by IbAINV1 was the main AI that degraded sucrose in the tuberous roots of both cultivars. IbAINV1 gene expression increased transiently and reached a maximum (2.3-times the pre-treatment value) at 20 d in 'Beniazuma', whereas it decreased to approx. 0.14- times the pre-treatment value by 10 d in 'Kokei 14' and remained low for the duration of the experiment. Sucrose concentrations did not change in the tuberous roots of 'Kokei 14' stored at 13°C for 20 d.Therefore, we conclude that the accumulation of sucrose in the tuberous roots of 'Kokei 14' was caused mainly by suppression of IbAINV1 gene expression in response to chilling.


Fukuoka N.,Ishikawa Prefectural University | Suzuki T.,Ishikawa Prefectural University | Minamide K.,Ishikawa Prefectural University | Minamide K.,Ishikawa Agricultural Research Center | Hamada T.,Ishikawa Prefectural University
HortScience | Year: 2014

The effect of shading in midsummer on anthocyanin and non-flavonoid polyphenol biosynthesis of Gynura bicolor DC leaves was examined using a control (full solar radiation) and a shade treatment (50% shading of full solar radiation). Leaf temperature in the shade plot remained ∼40 °C in the daytime, ∼6 °C lower than in the control. Plants in the shade plot grew better than the control. The content of chlorogenic acid (CGA) in leaves decreased with leaf maturation for both treatments, and a larger amount of CGA was detected in leaves from the control than the shade treatment. The profiles of reactive oxygen species (ROS) scavenging activity exhibited an identical pattern to the content of CGA. Although there was an abrupt increase in the content of anthocyanin in the early stage of leaf expansion, the content decreased rapidly as the leaves matured. The increase in anthocyanin early during leaf expansion was much more limited in control leaves than shaded leaves. There were no correlation between the profiles of anthocyanin and gene expression such as GbPAL, GbC4H, Gb4CL, GbCHS, GbCHI, GbF3H, and GbUFGT. However, the profiles of expression of genes such as GbMYB2, GbF3#H, GbDFR, and GbANS were similar to the anthocyanin profiles. These results suggest that artificial shading in midsummer is an effective method to promote anthocyanin accumulation but reduces ROS scavenging capacity as a result of lowered CGA production. © 2014, HORTSCIENCE. All rights reserved.


Hatakeyama K.,National Institute of Vegetable and Tea Science NIVTS | Horisaki A.,Tohoku Seed Co. | Horisaki A.,Ishikawa Agricultural Research Center | Niikura S.,Tohoku Seed Co. | And 6 more authors.
Genome | Year: 2010

The level of self-incompatibility (SI) is important to the purity of F1 seeds produced using the SI system of Brassica vegetables. To analyze the genetic basis of the level of SI, we generated an F2 population derived from a cross between a turnip inbred line showing a high level of SI and a Chinese cabbage inbred line showing a low level, and evaluated the level of SI under insect pollination in two years. We constructed a detailed linkage map of Brassica rapa from the F2 progeny, consisting of SSR, SNP, indel, and CAPS loci segregating into 10 linkage groups covering approximately 700 cM. Five quantitative trait loci (QTL) for high-level SI were identified. The phenotypic variation explained by the QTL ranged between 7.2% and 23.8%. Two QTL were detected in both years. Mapping of SI-related genes revealed that these QTL were co-localized with SLG on R07 and MLPK on R03. This is the first report of QTL for high-level SI evaluated under insect pollination in a Brassica vegetable. Our results could be useful for the marker-assisted selection of parental lines with a stable SI.


Adachi N.,Ishikawa Agricultural Research Center | Tsukamoto S.,Ishikawa Agricultural Research Center | Inoue Y.,Japan National Agricultural Research Center | Azegami K.,Japan National Agricultural Research Center
Plant Disease | Year: 2012

In Japan, rice seed are immersed in pesticide solutions to prevent seedborne diseases that attack greenhouse seedlings. However, disposal of large quantities of waste pesticide solutions after treatment is costly. As an alternative treatment, bacteriophages (phages) that are highly specific to the target bacteria are considered as potential biocontrol agents. Here, we isolated three phage strains that lyse Burkholderia glumae and B. plantarii, the causative pathogens of seedling rot and seedling blight, respectively. Two phages could lyse both bacteria and clearly suppress these diseases. One of these phages (BGPP-Ar) suppressed these diseases more effectively than existing pesticides: the ratio of seedlings exhibiting disease to the total number of seedlings examined after treatment with BGPP-Ar 1.0 × 108 plaque-forming units (PFU)/ml was 0.0 for seedling rot and 2.0 for seedling blight; after treatment with ipconazole/copper (II) hydroxide, the ratios were 14.3 and 15.0, respectively. BGPP-Ar was highly effective in suppressing seedling rot of rice, even at the low concentration of 1.0 × 105 PFU/ml. The best phage treatment effect for sterilizing seed is achieved indoors to avoid phage inactivation by UV irradiation. Treatment effect was demonstrated on seed infected with pathogens. Therefore, we consider that phage treatment was effective in this study. © 2012 The American Phytopathological Society.


PubMed | Ishikawa Agricultural Research Center
Type: Journal Article | Journal: Bioscience, biotechnology, and biochemistry | Year: 2011

A starch ingredient with antioxidative activity, as measured by the DPPH method, was produced by baking corn starch with an organic acid; it has been named ANOX sugar (antioxidative sugar). The baking temperature and time were fixed at 170 C and 60 min, and the organic acid used was selected from preliminary trials of various kinds of acid. The phytic acid ANOX sugar preparation showed the highest antioxidative activity, but the color of the preparation was almost black; we therefore selected L-tartaric acid which had the second highest antioxidative activity. The antioxidative activity of the L-tartaric acid ANOX sugar preparation was stable against temperature, light, and enzyme treatments (-amylase and glucoamylase). However, the activity was not stable against variations in water content and pH value. The antioxidative activity of ANOX sugar was stabilized by treating with boiled water or nitrogen gas, or by pH adjustment.

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