Sant Carles de la Ràpita, Spain
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Mateus A.P.,University of Algarve | Costa R.A.,University of Algarve | Cardoso J.C.R.,University of Algarve | Andree K.B.,IRTA SCR | And 3 more authors.
Journal of Endocrinology | Year: 2017

The impact of thermal imprinting on the plasticity of the hypothalamic-pituitary- interrenal (HPI) axis and stress response in an adult ectotherm, the gilthead sea bream (Sparus aurata, L.), during its development was assessed. Fish were reared under 4 thermal regimes, and the resulting adults exposed to acute confinement stress and plasma cortisol levels and genes of the HPI axis were monitored. Changes in immune function, a common result of stress, were also evaluated using histomorphometric measurements of melanomacrophages centers (MMCs) in the head kidney and by monitoring macrophage-related transcripts. Thermal history significantly modified the HPI responsiveness in adult sea bream when eggs and larvae were reared at a higher than optimal temperature (HT, 22°C), and they had a reduced amplitude in their cortisol response and significantly upregulated pituitary pomc and head kidney star transcripts. Additionally, after an acute stress challenge, immune function was modified and the head kidney of adult fish reared during development at high temperatures (HT and LHT, 18-22°C) had a decreased number of MMCs and a significant downregulation of dopachrome tautomerase. Thermal imprinting during development influenced adult sea bream physiology and increased plasma levels of glucose and sodium even in the absence of an acute stress in fish reared under a high-low thermal regime (HLT, 22-18°C). Overall, the results demonstrate that temperature during early development influences the adult HPI axis and immune function in a teleost fish. © 2017 The authors.

Boglione C.,University of Rome Tor Vergata | Gisbert E.,IRTA SCR | Gavaia P.,University of Algarve | Witten P.E.,Ghent University | And 3 more authors.
Reviews in Aquaculture | Year: 2013

The presence of skeletal anomalies in farmed teleost fish is currently a major problem in aquaculture, entailing economical, biological and ethical issues. The common occurrence of skeletal abnormalities in farmed fish and the absence of effective solutions for avoiding their onset or definitely culling out the affected individuals as early as possible from the productive cycle, highlight the need to improve our knowledge on the basic processes regulating fish skeletogenesis and skeletal tissues differentiation, modelling and remodelling. Severe skeletal anomalies may actually occur throughout the entire life cycle of fish, but their development often begins with slight aberrations of the internal elements. Comprehensive investigation efforts conducted on reared larvae and juveniles could provide a great contribution in filling the gap in knowledge, as skeletogenesis and skeletal tissue differentiation occur during these early life stages. The aim of this review is to provide a synthetic but comprehensive picture of the actual knowledge on the ontogeny, typologies and occurrence of skeletal anomalies, and on the proposed causative factors for their onset in larvae and juveniles of European farmed fish. The state-of-art of knowledge of these issues is analysed critically intending to individualize the main gaps of knowledge that require to be filled, in order to optimize the morphological quality of farmed juveniles. © 2013 Wiley Publishing Asia Pty Ltd.

Boglione C.,University of Rome Tor Vergata | Gavaia P.,University of Algarve | Koumoundouros G.,University of Crete | Gisbert E.,IRTA SCR | And 3 more authors.
Reviews in Aquaculture | Year: 2013

This critical review summarizes the knowledge about fish skeletal tissues and inherent normal and anomalous development. Particular emphasis is given to existing literature on reared European fishes. The aim was to identify the main gaps of knowledge that require to be filled, in order to precociously identify anomalous developmental patterns that lead to skeletal anomalies in reared finfish larvae and juveniles. The review also aims to extend our knowledge about the factors that are possibly involved in the onset of skeletal anomalies. The final goal is the optimization of the morphological quality of farmed juvenile fish. © 2013 Wiley Publishing Asia Pty Ltd.

Lopez-Joven C.,IRTA SCR | Lopez-Joven C.,University of Zaragoza | Ruiz-Zarzuela I.,University of Zaragoza | de Blas I.,University of Zaragoza | And 2 more authors.
Food Microbiology | Year: 2011

Effective post-harvest treatment to eliminate toxigenic vibrios is an important measure to reduce risk associated with seafood consumption. In the present study, we examined whether natural populations of sucrose nonfermenting (SNFV) and sucrose fermenting vibrio. s (SFV) persisted in Ruditapes philippinarum, after depuration at two different temperatures. Two experiments (one in Spring and one in Summer) were performed. Clams were depurated in duplicate tanks in an open-circuit seawater system using filtration and ultraviolet treatment. Clams were sampled daily (9 days), individually processed and inoculated on thiosulphate-citrate-bile salts-sucrose (TCBS) agar plates to estimate the density of SNFV and SFV. Results showed that depuration was less effective in Summer than in Spring, for SNFV and SFV. Despite an initial lower presence in the Summer experiment, clearance of SNFV was less efficient than in Spring, with 22.2% of clams presenting SNFV on day 8 and without significant changes on day 9. When compared, daily proportion of presence of SNFV and SFV in Spring, it was observed that SFV were more frequent than SNFV on days 8 and 9. In Summer these differences were significant on days 7-9. Present study demonstrated that SNFV (involved in food poisoning) are purged, at any temperature, faster than SFV. © 2011 Elsevier Ltd.

Suffredini E.,Instituto Superiore Of Sanita | Lopez-Joven C.,IRTA SCR | Lopez-Joven C.,University of Zaragoza | Maddalena L.,Instituto Superiore Of Sanita | And 2 more authors.
Applied and Environmental Microbiology | Year: 2011

The present study used pulsed-field gel electrophoresis (PFGE) characterization to examine the intraspecies variability and genetic relationships among environmental isolates of Vibrio parahaemolyticus from different European countries. This is first study performed on environmental V. parahaemolyticus that included more than one European country. © 2011, American Society for Microbiology.

Boglino A.,IRTA SCR | Darias M.J.,IRD Montpellier | Andree K.B.,IRTA SCR | Estevez A.,IRTA SCR | Gisbert E.,IRTA SCR
Journal of Applied Ichthyology | Year: 2014

Flatfish can provide a reliable model to study developmental disorders in bone tissues occurring during morphogenesis in response to nutritional imbalances. To date, most studies dealing with the effect of dietary essential fatty acids (EFA) on skeletogenesis in fish have focused their investigation on the role of docohexanoic (22:6n-3, DHA) and eicosapentaenoic (20:5n-3, EPA) acids, but only a few have focused on investigating the effects of arachidonic acid (20:4n-6, ARA) on bone during fish larval development. Bone development and composition at larval stage have been demonstrated to be highly sensitive to dietary levels of EFA, in particular the EPA and ARA acids, both precursors for highly bioactive eicosanoids presenting opposite effects on bone metabolism. Since fish are not able to synthesize EFA, they need to obtain them from the diet. However, dietary imbalances in EPA and ARA in flatfish larvae may disrupt bone formation and osteoblast differentiation in skeletal tissues, leading to the incidence of skeletal deformities, reduced mineralization and problems of bone remodelling in the cranial region associated with impaired eye migration. These anomalies in skeletal structures are one of the most important factors that affect flatfish larval quality and hamper their production. Thus, we have reviewed the current state of knowledge about the effects of dietary ARA contents on skeletogenesis in Senegalese sole (Solea senegalensis), one of the main flatfish species cultured in Europe. Their larval quality still suffers for a high incidence of skeletal anomalies induced by dietary imbalances during metamorphosis. © 2014 Blackwell Verlag GmbH.

Boglino A.,IRTA SCR | Darias M.J.,IRTA SCR | Darias M.J.,IRD Montpellier | Estevez A.,IRTA SCR | And 2 more authors.
Journal of Applied Ichthyology | Year: 2012

To improve the understanding of the incidence of skeletal deformities in Senegalese sole (Solea senegalensis), a feeding trial was carried out to evaluate the effect of increasing dietary arachidonic acid (ARA) levels during the Artemia feeding period on larval growth and skeletogenesis. Larvae were fed from 8 to 50days post hatching (dph) with Artemia nauplii enriched with three different levels of ARA: ARA-Low, ARA-Medium and ARA-High (1.0, 4.5 and 7.0% ARA of total fatty acids, respectively). Increasing levels of dietary ARA did not affect significantly the survival. However, it influenced growth; larvae from the ARA-M group presented the highest values of final standard length and dry weight (11.36±0.47mm and 5.86±1.06mg at 50dph, respectively) and larvae from the ARA-H group the lowest ones (9.53±0.27mm and 2.47±0.26mg at 50dph). The skeleton of larvae fed the ARA-M diet tended to be more calcified at 15dph than that of larvae fed ARA-L and ARA-H diets. Larvae from the ARA-M group tended to show a higher incidence of fusion of hypurals 3 and 4 (34.4±3.1%) at 50dph than the larvae from the other groups (18.7±1.6%, in average). The latter results reflected a trend to a higher degree of skeletal development rather than a deformity, this being in agreement with the higher larval growth of this group. Besides, ARA levels did not affect significantly the incidence of total skeletal deformities (41.4±1.5% in average). Larvae from all dietary treatments mostly displayed fusions of the vertebra 43 and 44 (32.2±1.3% in average). The amount of 4.5% of total fatty acid of dietary ARA during Artemia feeding period promoted the best growth and proper skeletogenesis in Senegalese sole larvae. © 2012 Blackwell Verlag, Berlin.

PubMed | IRTA SCR, Research Center en Alimentacion y Desarrollo, 73 rue de la Fee au Bois, Virginia Institute of Marine Science and Universidad Michoacana de San Nicolás de Hidalgo
Type: | Journal: Journal of invertebrate pathology | Year: 2015

The world population is growing quickly and there is a need to make sustainable protein available through an integrated approach that includes marine aquaculture. Seafood is already a highly traded commodity but the production from capture fisheries is rarely sustainable, which makes mollusc culture more important. However, an important constraint to its continued expansion is the potential for trade movements to disseminate pathogens that can cause disease problems and loss of production. Therefore, this review considers legislative and regulatory aspects of molluscan health management that have historically attempted to control the spread of mollusc pathogens. It is argued that the legislation has been slow to react to emerging diseases and the appearance of exotic pathogens in new areas. In addition, illegal trade movements are taken into account and possible future developments related to improvements in areas such as data collection and diagnostic techniques, as well as epidemiology, traceability and risk analysis, are outlined.

PubMed | IRTA SCR and Russian Academy of Sciences
Type: Journal Article | Journal: FEMS microbiology ecology | Year: 2016

In this investigation, we examined the influence of different DNA extraction protocols on results obtained for intestinal microbiota of Prussian carp. We showed that significant differences were observed in numbers of reads, OTUs, Shannon index and taxonomic composition between two different DNA extraction protocols for intestine of Prussian carp (Carassius gibelio), and differences were also evident between microbial communities in the intestinal mucosa and intestinal content. Statistical analyses of 25 published articles also revealed a significant relationship between methods of DNA extraction and bacterial diversity in fish intestine of freshwater species. Microbial diversity, community structure, proportions of read numbers derived from each OTU and the total number of OTUs obtained by different DNA extraction protocols could lead to a bias in results obtained in some cases, and therefore researchers should be conservative in conclusions about community structures.

Padros F.,Autonomous University of Barcelona | Villalta M.,IRTA SCR | Gisbert E.,IRTA SCR | Estevez A.,IRTA SCR
Journal of Fish Biology | Year: 2011

This study provides a comprehensive description of the main morphological and histological events that take place during larval and post-larval development of Senegal sole Solea senegalensis in order to establish a reference for its normal developmental organogenesis. Five stages have been described. Before gill development at the onset of metamorphosis (eye migration process, stage 4c), the skin was the main site of gas and ion exchange, whereas during stages 3 and 4, the skin begins differentiating into the definitive juvenile structure. The timing of development of the endocrine system depends on each organ, the endocrine pancreas and thyroid gland being the first to differentiate (stages 2 and 3, respectively), followed by the interrenal tissue and stannius corpuscles that develop at metamorphosis (stages 4 and 4c, respectively). The differentiation and maturation of the lymphohaematopoietic organs was coupled with the increase in complexity of the cardiovascular system and the presence of mature erythrocytes (stage 4b), which might be attributed to the change in respiration and the development of fully functional gills. In the differentiation of sensory structures, the development of eyes, inner ear, neuromasts and olfactory organs was rapid, with most of these organs becoming fully developed soon after hatching (stage 1). Vision, chemo- and mechano-reception developed very early in ontogeny, in parallel with the development of the central nervous system and changes in feeding habits. Although the general pattern of development in S. senegalensis appeared similar to most marine fish larvae already described, there were species-specific ontogenetic characteristics probably derived from the species' particular environment (subtropical waters) and behaviour (nocturnal, benthic, omnivorous feeding habits). These results on the organogenesis of larvae are a useful tool for establishing the functional systemic capabilities and physiological requirements of larvae to ensure optimal welfare and growth under aquaculture conditions. © 2011 The Authors. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles.

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