Irrigated Agriculture Research and Extension Center

WA, United States

Irrigated Agriculture Research and Extension Center

WA, United States
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Wu M.,Fujian Agriculture and forestry University | Wu M.,Washington State University | Gotoh H.,Washington State University | Waters T.,Washington State University | And 3 more authors.
Pest Management Science | Year: 2014

BACKGROUND: Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid-resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations. RESULTS: Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda-cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage-gated sodium channel gene. One mutation resulted in the non-synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non-synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda-cyhalothrin resistance. CONCLUSION: We have characterized a known kdr mutation and identified a novel mutation in the voltage-gated sodium channel gene of Thrips tabaci associated with resistance to lambda-cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations. © 2013 Society of Chemical Industry.

Oyarzun R.,University of La Serena | Stockle C.,Washington State University | Whiting M.,Irrigated Agriculture Research and Extension Center
Chilean Journal of Agricultural Research | Year: 2010

As a necessary step towards understanding soil water extraction and plant water relationships, the components of hydraulic conductance (K) of mature sweet cherry (Prunus avium L.) trees were evaluated in situ based on a Ohm ́s law analog method. In June 2004, K was determined for 10-yr-old 'Bing'/'Gisela® 5' trees, from simultaneous measurements of whole canopy gas exchange and leaf (sunlit and shaded) and stem water potentials (Ψ). Leaf water potential of sunlit leaves was lower than shaded leaves, reaching minimum values of ca. -2.3 MPa around 14:00 h (solar time). Average total hydraulic conductance was 60 ± 6 mmol s-1 MPa-1, presenting a slight decreasing trend as the season progressed. The analysis of tree K components showed that it was higher on the stem-leaf pathway (150 ± 50 mmol s-1 MPa-1), compared to the root-stem component (100 ± 20 mmol s-1 MPa-1), which is in agreement with literature reports for other fruit trees. A weak hysteresis pattern in the daily relationship between whole-canopy transpiration (weighted sunlit and shaded leaves) vs. Ψ was observed, suggesting that water storage within the tree is not a significant component of sweet cherry water balance.

Zhou C.,Washington State University | Kandemir I.,Ankara University | Walsh D.B.,Washington State University | Walsh D.B.,Irrigated Agriculture Research and Extension Center | And 2 more authors.
PLoS ONE | Year: 2012

Background: The western tarnished plant bug Lygus hesperus is an economically important pest that belongs to a complex of morphologically similar species that makes identification problematic. The present study provides evidence for the use of DNA barcodes from populations of L. hesperus from the western United States of America for accurate identification. Methodology/Principal Findings: This study reports DNA barcodes for 134 individuals of the western tarnished plant bug from alfalfa and strawberry agricultural fields in the western United States of America. Sequence divergence estimates of <3% reveal that morphologically variable individuals presumed to be L. hesperus were accurately identified. Paired estimates of F st and subsequent estimates of gene flow show that geographically distinct populations of L. hesperus are genetically similar. Therefore, our results support and reinforce the relatively recent (<100 years) migration of the western tarnished plant bug into agricultural habitats across the western United States. Conclusions/Significance: This study reveals that despite wide host plant usage and phenotypically plastic morphological traits, the commonly recognized western tarnished plant bug belongs to a single species, Lygus hesperus. In addition, no significant genetic structure was found for the geographically diverse populations of western tarnished plant bug used in this study. © 2012 Zhou et al.

Kimura E.,Texas AgriLife Research Center | Fransen S.C.,Irrigated Agriculture Research and Extension Center | Collins H.P.,U.S. Department of Agriculture | Guy S.O.,Washington State University | Johnston W.J.,Washington State University
Biomass and Bioenergy | Year: 2015

Switchgrass (Panicum virgatum L.) is a perennial warm-season grass identified as a model species for bioenergy feedstock production. Established switchgrass stands are very resilient to the environmental fluctuations; however, seed dormancy and weak seedling vigor make establishment difficult. Breaking seed dormancy of switchgrass is a first step to reduce risk of establishment failure and the costs associated with reseeding. Many studies have reported a myriad of methods to break seed dormancy of switchgrass, including chemical, mechanical, thermal, and hormonal seed treatments. Length of seed storage, storage conditions (e.g., temperature and humidity), and prior soil conditions (e.g., soil salinity and fertilizer rates) affect switchgrass seed dormancy. Strong interactions exist among germination, seedling emergence, and soil conditions; therefore, treated seeds tested in soil media will generate more accurate results following dormancy breaking techniques. This paper reviews the current methods used to break switchgrass seed dormancy. © 2015 Elsevier Ltd.

Felix J.,Oregon State University | Boydston R.,Irrigated Agriculture Research and Extension Center | Burke I.C.,Washington State University
Weed Technology | Year: 2012

Field studies were conducted in 2011 at the Malheur Experiment Station, Ontario, OR and Prosser, WA to evaluate the effect of simulated glyphosate drift on direct-seeded dry bulb onion. Glyphosate was applied at 8.6, 25.8, 86, 290, 434, and 860 g ae ha-1 when onion plants were at the flag-, two-, four-, and six-leaf stages. Onion foliar injury was directly related to the glyphosate dose and varied with application timing. Foliar injury at 7 d after treatment (DAT) ranged from 0 to 12% for glyphosate ≤25.8 g ha-1. Foliar injury increased at 21 DAT when glyphosate was applied ≥25.8 g ha -1 to plants at the flag- and four-leaf stage, and ranged from 24 to 99%. The 50%-injury glyphosate dose at 21 DAT was lowest when onion was treated at the four-leaf and flag stages and was estimated to be 76.8 and 81 g ha -1, respectively. Onion injury severity increased when glyphosate was applied at ≥86 g ha-1 and eventually resulted in plant death at 860 g ha-1. Foliar injury was inversely correlated to U.S. no. 1 onion yield. Onions displayed sensitivity to very low glyphosate doses especially at the four-leaf stage. Shikimic acid accumulation increased with the increase in glyphosate dose and was positively correlated with foliar injury and negatively correlated with plant height and onion yield. Nomenclature: Glyphosate; onion, Allium cepa L. 'Vaquero'.

Boydston R.A.,Irrigated Agriculture Research and Extension Center | Felix J.,Oregon State University | Al-Khatib K.,University of California at Davis
Weed Technology | Year: 2012

Field trials were conducted in 2009 and 2010 near Paterson, WA and Ontario, OR to evaluate weed control and potato tolerance to PRE-applied pyroxasulfone, saflufenacil, and KSU12800 herbicides. Pyroxasulfone at 0.09 to 0.15 kg ai ha-1 and saflufenacil at 0.05 to 0.07 kg ai ha-1 applied PRE alone or in tank mixes with several currently labeled herbicides did not injure potatoes at either site in both years. KSU12800 at 0.15 kg ai ha -1 injured potatoes from 18 to 26% for a period of about 4 wk after emergence at Ontario both years. In addition, KSU12800 at 0.29 and 0.45 kg ha-1 injured potatoes from 17 to 38% at 17 d after treatment (DAT) at Paterson in 2009. Pyroxasulfone at 0.15 kg ha-1 controlled barnyardgrass, hairy nightshade, and redroot pigweed 96% or greater, but control of common lambsquarters was variable. Saflufenacil at 0.07 kg ha-1 provided greater than 93% control of common lambsquarters, hairy nightshade, and redroot pigweed at both sites in 2010. KSU12800 at 0.15 kg ha-1 controlled common lambsquarters, hairy nightshade, and redroot pigweed 99% or more at Ontario, but only 87 to 93% at Paterson in 2010. These herbicides did not reduce yield of U.S. no. 1 tubers or total tuber yields compared to standard labeled herbicide treatments when weed control was adequate.

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